RLG00000019711

HAUS augmin-like complex subunit 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002315
Physical Location & Seq
Reverse (-)
55820817 .. 55825039
4223 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019711

Sequence Viewer

Length: 1296 bp
ATGGTGAAGGGAGTGCAACAAGGCCAAAATTTGCCGCCCGATTTGACCCAAGTCATTGATCAGTTGGAGCGCCACTGCTTGGCTCCGGATGGTTCCCTCGTCTCTAAATCCGCCTTCTACGACCTCCAACTGGCAAGAGAGGAAATGTCCAAGGAAAGACTGCGTTACTTGGAAGCAATGGCAATGGCAATGGTGGAAGAGTACCAGCAAGCGGTTTCGATGGCTAACCTTGGAGGAAGTCGAGATGTGCAGGGTGTCTATCAGCAATTGGGCTTGAACAACTCTCCACAGCATCGCATGATCATTGCAGAAGCAGCTCAAAGGTTGAGGCTTCCTCTGATCTCTAAAGATGGTGAAATTCATGAGGAAGAAATTGAGAAATGCAGTGTGTTGTCACGGACTTCCCTTTATAGTACTAGCACTGGTACAATCAGCTCAAGTTCCAACTCGACAAGCTATACTACTGCAACTGGCACCAGTAGTGCAGCAAACAACCATCTTTCACTTGGTGCTTCTGATGTTGTGGAACCTGGAGTTGGTGGTGTTCCCAATTGTTTTCTAGGAATCGCACCTGCTTTATTTATGGCAAACAAAGCTGCAGCAAACACCATTTCTCCCTGGTACAATGACATGACAGAATATCACGTGTCTCTTTCACGTGAGATTGAGGCTCGTTTGCAAGCTAAATGTGACAAGCTAGCTGATGCAGTTATAATGGATGACACTGGTACAAGTCTCTCTCTCTCTATTCCTTTCTCCCAATTAATAATTGAGGAGATGAAAAGGGATGAAGCAGCTTTTCAAGACGATCTATATTCTGCAGATAGAAAATTTGCTGAATATTATAACCAGATACTGGCAGTGCTTATTAAGCTGGTCAAAGATTTGAAGTTGCAGCATCAACATAAATATGAAGATCTGCAAAAAACATGGCTGTGCAAAAGGTGTGAGACCATGAGTGCAAAATTGAGGGTTCTGGAGAATGTTCTCCTTCTTGAAACTTATACTAACGAGTCAGTACCAGCCCTTCATAAAATAAGGAAATATCTTCTTGAAGCTACAGAAGAAGCTTCTATGGCTTATAATAAAGCGGTTACTCGTCTTCGAGAGTATCAGGGTGTTGATCCTCACTTCGATACAATTGCAAGGCAGTGCCACGATATTGTAAAGGGATGGAAATTGGCTTCCAATCTGACCAATCATGTGTACAATTCCGTCTGTACCAAGAACTTCATTTTACCACGTAAAATAGTTGCAGGCACACATTGTGTAATGACTTGTGAATGCATCTGTTGA

Protein Analysis

432

Amino Acids

48.12

Weight (kDa)

5.88

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS4 PF14735 175 - 390 1.5e-56 HAUS augmin-like complex subunit 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 713, 846, 1083
AarI CACCTGC 1 cut(s) 580
Acc36I ACCTGC 1 cut(s) 580
AccB1I GGYRCC 1 cut(s) 473
AccB7I CCANNNNNTGG 2 cut(s) 79, 856
AccIII TCCGGA 1 cut(s) 85
AciI CCGC 4 cut(s) 35, 111, 212, 1091
AclWI GGATC 1 cut(s) 1118
AcsI RAATTY 3 cut(s) 28, 357, 830
AcvI CACGTG 2 cut(s) 646, 659
AdeI CACNNNGTG 2 cut(s) 509, 1268
AfaI GTAC 8 cut(s) 203, 415, 427, 623, 730, 1020, 1208, 1222
AfiI CCNNNNNNNGG 5 cut(s) 79, 130, 211, 536, 856
AflIII ACRYGT 1 cut(s) 645
AgsI TTSAA 5 cut(s) 277, 803, 889, 998, 1055
AjnI CCWGG 2 cut(s) 529, 617
AjuI GAANNNNNNNTTGG 2 cut(s) 519, 551
Alw26I GTCTC 4 cut(s) 106, 654, 740, 944
AlwI GGATC 1 cut(s) 1118
AlwNI CAGNNNCTG 1 cut(s) 856
Aor13HI TCCGGA 1 cut(s) 85
AoxI GGCC 1 cut(s) 22
ApeKI GCWGC 6 cut(s) 314, 485, 596, 599, 794, 895
ApoI RAATTY 3 cut(s) 28, 357, 830
AseI ATTAAT 1 cut(s) 764
AspLEI GCGC 1 cut(s) 72
AsuHPI GGTGA 2 cut(s) 16, 365
AsuNHI GCTAGC 1 cut(s) 697
BanI GGYRCC 1 cut(s) 473
BarI GAAGNNNNNNTAC 2 cut(s) 1011, 1043
BbrPI CACGTG 2 cut(s) 646, 659
BbsI GAAGAC 1 cut(s) 1094
BbvI GCAGC 6 cut(s) 326, 497, 583, 611, 806, 907
BccI CCATC 5 cut(s) 83, 214, 344, 504, 1167
BcgI CGANNNNNNTGC 2 cut(s) 1124, 1158
BciT130I CCWGG 2 cut(s) 531, 619
BclI TGATCA 2 cut(s) 58, 300
BcoDI GTCTC 4 cut(s) 106, 654, 740, 944
BfaI CTAG 3 cut(s) 417, 560, 698
BfmI CTRYAG 3 cut(s) 597, 819, 1059
BfoI RGCGCY 1 cut(s) 73
BfuAI ACCTGC 1 cut(s) 580
BglII AGATCT 1 cut(s) 916
BisI GCNGC 7 cut(s) 35, 315, 486, 597, 600, 795, 896
BlsI GCNGC 7 cut(s) 36, 316, 487, 598, 601, 796, 897
BmcAI AGTACT 1 cut(s) 415
Bme1390I CCNGG 2 cut(s) 531, 619
BmiI GGNNCC 4 cut(s) 84, 94, 475, 528
BmrFI CCNGG 2 cut(s) 531, 619
BmsI GCATC 3 cut(s) 301, 694, 907
BmtI GCTAGC 1 cut(s) 701
BoxI GACNNNNGTC 1 cut(s) 50
BpiI GAAGAC 1 cut(s) 1094
BplI GAGNNNNNCTC 2 cut(s) 319, 351
BpmI CTGGAG 2 cut(s) 552, 998
BpuEI CTTGAG 1 cut(s) 421
BsaAI YACGTR 3 cut(s) 646, 659, 1244
BsaI GGTCTC 1 cut(s) 944
BsaJI CCNNGG 3 cut(s) 150, 229, 617
BsaWI WCCGGW 1 cut(s) 85
BsaXI ACNNNNNCTCC 2 cut(s) 598, 628
Bsc4I CCNNNNNNNGG 5 cut(s) 79, 130, 211, 536, 856
Bse1I ACTGG 6 cut(s) 135, 427, 475, 477, 730, 861
Bse3DI GCAATG 4 cut(s) 183, 189, 195, 303
BseAI TCCGGA 1 cut(s) 85
BseBI CCWGG 2 cut(s) 531, 619
BseDI CCNNGG 3 cut(s) 150, 229, 617
BseGI GGATG 4 cut(s) 94, 724, 793, 1178
BseLI CCNNNNNNNGG 5 cut(s) 79, 130, 211, 536, 856
BseMI GCAATG 4 cut(s) 183, 189, 195, 303
BseNI ACTGG 6 cut(s) 135, 427, 475, 477, 730, 861
BseRI GAGGAG 1 cut(s) 788
BseXI GCAGC 6 cut(s) 326, 497, 583, 611, 806, 907
BsgI GTGCAG 2 cut(s) 269, 504
BshFI GGCC 1 cut(s) 24
BshNI GGYRCC 1 cut(s) 473
BsiSI CCGG 1 cut(s) 86
BslI CCNNNNNNNGG 5 cut(s) 79, 130, 211, 536, 856
BsmAI GTCTC 4 cut(s) 106, 654, 740, 944
BsmBI CGTCTC 1 cut(s) 106
BsmI GAATGC 1 cut(s) 1289
BsnI GGCC 1 cut(s) 24
Bso31I GGTCTC 1 cut(s) 944
Bsp13I TCCGGA 1 cut(s) 85
Bsp1407I TGTACA 1 cut(s) 1206
Bsp143I GATC 6 cut(s) 58, 300, 339, 808, 916, 1123
BspACI CCGC 4 cut(s) 35, 111, 212, 1091
BspANI GGCC 1 cut(s) 24
BspEI TCCGGA 1 cut(s) 85
BspHI TCATGA 1 cut(s) 361
BspLI GGNNCC 4 cut(s) 84, 94, 475, 528
BspMAI CTGCAG 2 cut(s) 601, 823
BspMI ACCTGC 1 cut(s) 580
BspOI GCTAGC 1 cut(s) 701
BspPI GGATC 1 cut(s) 1118
BspT107I GGYRCC 1 cut(s) 473
BspTNI GGTCTC 1 cut(s) 944
BsrDI GCAATG 4 cut(s) 183, 189, 195, 303
BsrGI TGTACA 1 cut(s) 1206
BsrI ACTGG 6 cut(s) 135, 427, 475, 477, 730, 861
BssECI CCNNGG 3 cut(s) 150, 229, 617
BssMI GATC 6 cut(s) 58, 300, 339, 808, 916, 1123
BssT1I CCWWGG 2 cut(s) 150, 229
Bst2UI CCWGG 2 cut(s) 531, 619
Bst6I CTCTTC 1 cut(s) 192
BstAUI TGTACA 1 cut(s) 1206
BstBAI YACGTR 3 cut(s) 646, 659, 1244
BstC8I GCNNGC 4 cut(s) 210, 681, 699, 1258
BstF5I GGATG 4 cut(s) 94, 724, 793, 1178
BstH2I RGCGCY 1 cut(s) 73
BstHHI GCGC 1 cut(s) 72
BstKTI GATC 6 cut(s) 61, 303, 342, 811, 919, 1126
BstMAI GTCTC 4 cut(s) 106, 654, 740, 944
BstMBI GATC 6 cut(s) 58, 300, 339, 808, 916, 1123
BstMWI GCNNNNNNNGC 4 cut(s) 314, 593, 871, 1076
BstNI CCWGG 2 cut(s) 531, 619
BstPAI GACNNNNGTC 1 cut(s) 50
BstSCI CCNGG 2 cut(s) 529, 617
BstSFI CTRYAG 3 cut(s) 597, 819, 1059
BstV1I GCAGC 6 cut(s) 326, 497, 583, 611, 806, 907
BstV2I GAAGAC 1 cut(s) 1094
BstX2I RGATCY 1 cut(s) 916
BstYI RGATCY 1 cut(s) 916
BsuRI GGCC 1 cut(s) 24
BtgZI GCGATG 1 cut(s) 278
BtsCI GGATG 4 cut(s) 94, 724, 793, 1178
BtsI GCAGTG 4 cut(s) 73, 391, 867, 1157
BtsIMutI CAGTG 6 cut(s) 73, 391, 420, 723, 867, 1157
BveI ACCTGC 1 cut(s) 580
Cac8I GCNNGC 4 cut(s) 210, 681, 699, 1258
CaiI CAGNNNCTG 1 cut(s) 856
CciI TCATGA 1 cut(s) 361
CfoI GCGC 1 cut(s) 72
Csp6I GTAC 8 cut(s) 202, 414, 426, 622, 729, 1019, 1207, 1221
CviAII CATG 6 cut(s) 298, 362, 631, 930, 955, 1202
CviQI GTAC 8 cut(s) 202, 414, 426, 622, 729, 1019, 1207, 1221
DpnI GATC 6 cut(s) 60, 302, 341, 810, 918, 1125
DpnII GATC 6 cut(s) 58, 300, 339, 808, 916, 1123
DraIII CACNNNGTG 2 cut(s) 509, 1268
Eam1104I CTCTTC 1 cut(s) 192
EarI CTCTTC 1 cut(s) 192
EciI GGCGGA 1 cut(s) 100
Eco130I CCWWGG 2 cut(s) 150, 229
Eco31I GGTCTC 1 cut(s) 944
Eco72I CACGTG 2 cut(s) 646, 659
EcoRII CCWGG 2 cut(s) 529, 617
EcoT14I CCWWGG 2 cut(s) 150, 229
EcoT22I ATGCAT 1 cut(s) 1289
ErhI CCWWGG 2 cut(s) 150, 229
Esp3I CGTCTC 1 cut(s) 106
FaeI CATG 6 cut(s) 301, 365, 634, 933, 958, 1205
FatI CATG 6 cut(s) 297, 361, 630, 929, 954, 1201
FbaI TGATCA 2 cut(s) 58, 300
Fnu4HI GCNGC 7 cut(s) 35, 315, 486, 597, 600, 795, 896
FokI GGATG 4 cut(s) 101, 731, 800, 1185
Fsp4HI GCNGC 7 cut(s) 35, 315, 486, 597, 600, 795, 896
FspBI CTAG 3 cut(s) 417, 560, 698
GlaI GCGC 1 cut(s) 71
GluI GCNGC 7 cut(s) 35, 315, 486, 597, 600, 795, 896
GsuI CTGGAG 2 cut(s) 552, 998
HaeII RGCGCY 1 cut(s) 73
HaeIII GGCC 1 cut(s) 24
HapII CCGG 1 cut(s) 86
HhaI GCGC 1 cut(s) 72
Hin1II CATG 6 cut(s) 301, 365, 634, 933, 958, 1205
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HindIII AAGCTT 1 cut(s) 1068
HinfI GANTC 2 cut(s) 564, 1013
HpaII CCGG 1 cut(s) 86
HphI GGTGA 2 cut(s) 16, 365
Hpy166II GTNNAC 1 cut(s) 1207
Hpy188I TCNGA 3 cut(s) 339, 517, 1194
Hpy188III TCNNGA 8 cut(s) 86, 242, 362, 803, 977, 995, 1052, 1106
Hpy8I GTNNAC 1 cut(s) 1207
HpyAV CCTTC 3 cut(s) 124, 1001, 1037
HpyCH4IV ACGT 3 cut(s) 645, 658, 1243
HpyF10VI GCNNNNNNNGC 4 cut(s) 314, 593, 871, 1076
HpySE526I ACGT 3 cut(s) 645, 658, 1243
Hsp92II CATG 6 cut(s) 301, 365, 634, 933, 958, 1205
HspAI GCGC 1 cut(s) 70
Kpn2I TCCGGA 1 cut(s) 85
Ksp22I TGATCA 2 cut(s) 58, 300
Kzo9I GATC 6 cut(s) 58, 300, 339, 808, 916, 1123
LmnI GCTCC 2 cut(s) 67, 88
Lsp1109I GCAGC 6 cut(s) 326, 497, 583, 611, 806, 907
LweI GCATC 3 cut(s) 301, 694, 907
MaeI CTAG 3 cut(s) 417, 560, 698
MaeII ACGT 3 cut(s) 645, 658, 1243
MaeIII GTNAC 4 cut(s) 164, 393, 689, 1093
MalI GATC 6 cut(s) 60, 302, 341, 810, 918, 1125
MboI GATC 6 cut(s) 58, 300, 339, 808, 916, 1123
MboII GAAGA 6 cut(s) 209, 380, 926, 1040, 1076, 1094
MfeI CAATTG 3 cut(s) 266, 550, 1140
MflI RGATCY 1 cut(s) 916
MlyI GAGTC 1 cut(s) 1022
MmeI TCCRAC 3 cut(s) 45, 151, 468
Mph1103I ATGCAT 1 cut(s) 1289
MroI TCCGGA 1 cut(s) 85
MseI TTAA 2 cut(s) 764, 870
MslI CAYNNNNRTG 2 cut(s) 909, 934
MspI CCGG 1 cut(s) 86
MspR9I CCNGG 2 cut(s) 531, 619
MunI CAATTG 3 cut(s) 266, 550, 1140
Mva1269I GAATGC 1 cut(s) 1289
MvaI CCWGG 2 cut(s) 531, 619
MwoI GCNNNNNNNGC 4 cut(s) 314, 593, 871, 1076
NdeII GATC 6 cut(s) 58, 300, 339, 808, 916, 1123
NheI GCTAGC 1 cut(s) 697
NlaIII CATG 6 cut(s) 301, 365, 634, 933, 958, 1205
NlaIV GGNNCC 4 cut(s) 84, 94, 475, 528
NmuCI GTSAC 2 cut(s) 393, 689
NsiI ATGCAT 1 cut(s) 1289
PagI TCATGA 1 cut(s) 361
PaqCI CACCTGC 1 cut(s) 580
PctI GAATGC 1 cut(s) 1289
PfeI GAWTC 1 cut(s) 564
PflMI CCANNNNNTGG 2 cut(s) 79, 856
PkrI GCNGC 7 cut(s) 36, 316, 487, 598, 601, 796, 897
PleI GAGTC 1 cut(s) 1021
PmaCI CACGTG 2 cut(s) 646, 659
PmlI CACGTG 2 cut(s) 646, 659
PpsI GAGTC 1 cut(s) 1021
Ppu21I YACGTR 3 cut(s) 646, 659, 1244
PshAI GACNNNNGTC 1 cut(s) 50
PshBI ATTAAT 1 cut(s) 764
PsiI TTATAA 3 cut(s) 713, 846, 1083
Psp6I CCWGG 2 cut(s) 529, 617
PspCI CACGTG 2 cut(s) 646, 659
PspGI CCWGG 2 cut(s) 529, 617
PspN4I GGNNCC 4 cut(s) 84, 94, 475, 528
PstI CTGCAG 2 cut(s) 601, 823
PstNI CAGNNNCTG 1 cut(s) 856
PsuI RGATCY 1 cut(s) 916
RsaI GTAC 8 cut(s) 203, 415, 427, 623, 730, 1020, 1208, 1222
RsaNI GTAC 8 cut(s) 202, 414, 426, 622, 729, 1019, 1207, 1221
RseI CAYNNNNRTG 2 cut(s) 909, 934
SaqAI TTAA 2 cut(s) 764, 870
SatI GCNGC 7 cut(s) 35, 315, 486, 597, 600, 795, 896
Sau3AI GATC 6 cut(s) 58, 300, 339, 808, 916, 1123
ScaI AGTACT 1 cut(s) 415
SchI GAGTC 1 cut(s) 1022
ScrFI CCNGG 2 cut(s) 531, 619
SfaNI GCATC 3 cut(s) 301, 694, 907
SfcI CTRYAG 3 cut(s) 597, 819, 1059
SmiMI CAYNNNNRTG 2 cut(s) 909, 934
SmlI CTYRAG 1 cut(s) 436
SmoI CTYRAG 1 cut(s) 436
SsiI CCGC 4 cut(s) 35, 111, 212, 1091
SspI AATATT 1 cut(s) 842
SspMI CTAG 3 cut(s) 417, 560, 698
StyD4I CCNGG 2 cut(s) 529, 617
StyI CCWWGG 2 cut(s) 150, 229
TaiI ACGT 3 cut(s) 648, 661, 1246
TaqI TCGA 5 cut(s) 218, 241, 449, 1105, 1134
TatI WGTACW 2 cut(s) 413, 1206
TauI GCSGC 1 cut(s) 37
TfiI GAWTC 1 cut(s) 564
Tru1I TTAA 2 cut(s) 764, 870
Tru9I TTAA 2 cut(s) 764, 870
TscAI CASTG 6 cut(s) 80, 391, 427, 730, 867, 1157
TseFI GTSAC 2 cut(s) 393, 689
TseI GCWGC 6 cut(s) 314, 485, 596, 599, 794, 895
Tsp45I GTSAC 2 cut(s) 393, 689
TspDTI ATGAA 6 cut(s) 350, 794, 804, 927, 1019, 1222
TspGWI ACGGA 2 cut(s) 412, 1204
TspRI CASTG 6 cut(s) 80, 391, 427, 730, 867, 1157
Van91I CCANNNNNTGG 2 cut(s) 79, 856
VspI ATTAAT 1 cut(s) 764
XapI RAATTY 3 cut(s) 28, 357, 830
XcmI CCANNNNNNNNNTGG 1 cut(s) 503
XspI CTAG 3 cut(s) 417, 560, 698
ZrmI AGTACT 1 cut(s) 415
Zsp2I ATGCAT 1 cut(s) 1289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.