Rroxscaffold_2G00105540

HAUS augmin-like complex subunit 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002315
Physical Location & Seq
Reverse (-)
28955997 .. 28960273
4277 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00105540.1

Sequence Viewer

Length: 1299 bp
ATGGTGAAGGGAGTTCAACAAGGCCAAAACTTGCCGGCCGATTTGACCCAAGTCATTGATCAGTTGGAGCGCCACTGCTTGGCTCCCGATGGTTCCCTCGTCTCTAAATCCGCCTTCTACGACCTCCAACTGGCAAGGGAGGAAATGTCCAAGGAAAGACTGCGATACTTGGAAGCAATGGCACTGTATAGCGAGGCAATGGCAATGGTGGAAGAGTACCAGCAAGCGGTTTCGATGGCTAACCTTGGAGGAAGTCGAGATGTGCAGGGTGTGTATCAGCAATTGGGCTTGAAGAACTCTCCACAGCTGTATGAGACATTAGAGCATCGCATGATCGTTGCAGAAGCAGCTCAAAGGTTGAGGCTTCCTCTGATCTCTAAAGATGGTGAAATCCATGAGGAAGAAATTGAGAAATGCAGTGTTTTGTCACGGACTTCCCTTGATAGTACTAGCACTGGTACAATCAGCTCAAGTTCCAACTCGACAACCTACACTACTGCAACTGGCATCAGTAGTGCAGCAAACAACAATCTTTCACTTGGTGCTTCTGATGTTGTGGAACCTGGAGTTGGTGGTGTTCCCAATTGTTTTCTTGGAATTACACCTGCTTATCTATGGCAAACACAGCTGCAGCAAACACCATTCTCCCTGGATATGACAGAATATCAGGTGTCTCTTTCACGTGAGATGGAGGCTCGTTTGCAAGCTAAATGTGACAAGCTAGCTGATGCAGTTATAATGGATGATACTGACTCATCATCTGGACATCAAAATCTAAATGCTCGTCTTCCAGAGAGGGTAAAGTTAATAATTGAGGAGATGGAAAGGGATGAAGCAGCTCTTCAAGACGATCTATATTCTGCAGATAGAAAATTTGCTGAATATTATAACGTCTTAGAACAGATTCTGGCAGTGCTTATTAAGCTGGTCAAAGATTTGAAGTTGCAGCATCAACATAAATATGAAGATCTGCAAAAAACATGGCTGTGCAAAAGGTGTGAGACCATGAGTGCAAAATTGAGGGTTCTGGAGAATGTTCTCCTTCTTGAAACTTATACTAATGAGTCAATACCAGCCCTTCATAAAATAAGGAAATATCTTCTTGAAGCTACAGAAGAAGCTTCCATGGCTTATAATAAAGCGGTTACTCGTCTTCGAGAGTATCAGGGTGTTGATCCTCACTTCGATACAATTGCAAGGCAGTACCATGATATTGTAAAGAAATTGGAAAATATGCAATGGACAATCCACCAGGTTGAAATGGACCTAAAACGTCTCCCTGATCATGCAAATGCATAG

Protein Analysis

432

Amino Acids

48.84

Weight (kDa)

5.14

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS4 PF14735 186 - 421 3.6e-81 HAUS augmin-like complex subunit 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 737, 888, 1134
AarI CACCTGC 1 cut(s) 613
Acc36I ACCTGC 1 cut(s) 613
AccB7I CCANNNNNTGG 1 cut(s) 79
AciI CCGC 3 cut(s) 111, 227, 1142
AclWI GGATC 1 cut(s) 1169
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 1 cut(s) 872
AcvI CACGTG 1 cut(s) 683
AdeI CACNNNGTG 1 cut(s) 542
AfaI GTAC 4 cut(s) 218, 448, 460, 1205
AfiI CCNNNNNNNGG 4 cut(s) 79, 130, 226, 569
AgsI TTSAA 7 cut(s) 17, 292, 845, 940, 1049, 1106, 1259
AjnI CCWGG 3 cut(s) 562, 648, 1251
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
Alw26I GTCTC 5 cut(s) 106, 308, 678, 995, 1280
AlwI GGATC 1 cut(s) 1169
AlwNI CAGNNNCTG 1 cut(s) 907
AoxI GGCC 2 cut(s) 22, 36
ApeKI GCWGC 6 cut(s) 347, 518, 628, 631, 836, 946
ApoI RAATTY 1 cut(s) 872
Asp700I GAANNNNTTC 1 cut(s) 903
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 1 cut(s) 1264
AsuHPI GGTGA 2 cut(s) 16, 398
AsuNHI GCTAGC 1 cut(s) 721
AvaII GGWCC 1 cut(s) 1264
BbrPI CACGTG 1 cut(s) 683
BbsI GAAGAC 2 cut(s) 779, 1145
BbvI GCAGC 6 cut(s) 359, 530, 615, 643, 848, 958
BccI CCATC 5 cut(s) 83, 229, 377, 682, 814
BcgI CGANNNNNNTGC 2 cut(s) 1175, 1209
BciT130I CCWGG 3 cut(s) 564, 650, 1253
BclI TGATCA 2 cut(s) 58, 1282
BcoDI GTCTC 5 cut(s) 106, 308, 678, 995, 1280
BfaI CTAG 2 cut(s) 450, 722
BfmI CTRYAG 3 cut(s) 629, 861, 1110
BfoI RGCGCY 1 cut(s) 73
BfuAI ACCTGC 1 cut(s) 613
BglII AGATCT 1 cut(s) 967
BisI GCNGC 6 cut(s) 348, 519, 629, 632, 837, 947
BlsI GCNGC 6 cut(s) 349, 520, 630, 633, 838, 948
BmcAI AGTACT 1 cut(s) 448
Bme1390I CCNGG 3 cut(s) 564, 650, 1253
Bme18I GGWCC 1 cut(s) 1264
BmgT120I GGNCC 1 cut(s) 1264
BmiI GGNNCC 3 cut(s) 84, 94, 561
BmrFI CCNGG 3 cut(s) 564, 650, 1253
BmsI GCATC 4 cut(s) 334, 516, 718, 958
BmtI GCTAGC 1 cut(s) 725
BoxI GACNNNNGTC 1 cut(s) 50
BpiI GAAGAC 2 cut(s) 779, 1145
BplI GAGNNNNNCTC 2 cut(s) 352, 384
BpmI CTGGAG 2 cut(s) 585, 1049
BpuEI CTTGAG 1 cut(s) 454
BsaAI YACGTR 1 cut(s) 683
BsaI GGTCTC 1 cut(s) 995
BsaJI CCNNGG 4 cut(s) 150, 244, 648, 1125
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 4 cut(s) 79, 130, 226, 569
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 3 cut(s) 135, 460, 508
Bse3DI GCAATG 4 cut(s) 183, 204, 210, 1244
BseBI CCWGG 3 cut(s) 564, 650, 1253
BseDI CCNNGG 4 cut(s) 150, 244, 648, 1125
BseGI GGATG 2 cut(s) 748, 835
BseLI CCNNNNNNNGG 4 cut(s) 79, 130, 226, 569
BseMI GCAATG 4 cut(s) 183, 204, 210, 1244
BseNI ACTGG 3 cut(s) 135, 460, 508
BseRI GAGGAG 1 cut(s) 830
BseX3I CGGCCG 1 cut(s) 36
BseXI GCAGC 6 cut(s) 359, 530, 615, 643, 848, 958
BsgI GTGCAG 2 cut(s) 284, 537
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 2 cut(s) 24, 38
BsiEI CGRYCG 1 cut(s) 39
BsiSI CCGG 1 cut(s) 35
BslI CCNNNNNNNGG 4 cut(s) 79, 130, 226, 569
BsmAI GTCTC 5 cut(s) 106, 308, 678, 995, 1280
BsmBI CGTCTC 2 cut(s) 106, 1280
BsnI GGCC 2 cut(s) 24, 38
Bso31I GGTCTC 1 cut(s) 995
Bsp143I GATC 7 cut(s) 58, 333, 372, 850, 967, 1174, 1282
Bsp19I CCATGG 1 cut(s) 1125
BspACI CCGC 3 cut(s) 111, 227, 1142
BspANI GGCC 2 cut(s) 24, 38
BspLI GGNNCC 3 cut(s) 84, 94, 561
BspMAI CTGCAG 2 cut(s) 633, 865
BspMI ACCTGC 1 cut(s) 613
BspOI GCTAGC 1 cut(s) 725
BspPI GGATC 1 cut(s) 1169
BspQI GCTCTTC 1 cut(s) 846
BspTNI GGTCTC 1 cut(s) 995
BsrDI GCAATG 4 cut(s) 183, 204, 210, 1244
BsrFI RCCGGY 1 cut(s) 34
BsrI ACTGG 3 cut(s) 135, 460, 508
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 4 cut(s) 150, 244, 648, 1125
BssMI GATC 7 cut(s) 58, 333, 372, 850, 967, 1174, 1282
BssT1I CCWWGG 3 cut(s) 150, 244, 1125
Bst2UI CCWGG 3 cut(s) 564, 650, 1253
Bst4CI ACNGT 1 cut(s) 186
Bst6I CTCTTC 2 cut(s) 207, 846
BstBAI YACGTR 1 cut(s) 683
BstC8I GCNNGC 4 cut(s) 36, 225, 705, 723
BstDEI CTNAG 1 cut(s) 895
BstDSI CCRYGG 1 cut(s) 1125
BstF5I GGATG 2 cut(s) 748, 835
BstH2I RGCGCY 1 cut(s) 73
BstHHI GCGC 1 cut(s) 72
BstKTI GATC 7 cut(s) 61, 336, 375, 853, 970, 1177, 1285
BstMAI GTCTC 5 cut(s) 106, 308, 678, 995, 1280
BstMBI GATC 7 cut(s) 58, 333, 372, 850, 967, 1174, 1282
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 4 cut(s) 347, 625, 922, 1127
BstNI CCWGG 3 cut(s) 564, 650, 1253
BstPAI GACNNNNGTC 1 cut(s) 50
BstSCI CCNGG 3 cut(s) 562, 648, 1251
BstSFI CTRYAG 3 cut(s) 629, 861, 1110
BstV1I GCAGC 6 cut(s) 359, 530, 615, 643, 848, 958
BstV2I GAAGAC 2 cut(s) 779, 1145
BstX2I RGATCY 1 cut(s) 967
BstYI RGATCY 1 cut(s) 967
BstZI CGGCCG 1 cut(s) 36
BsuRI GGCC 2 cut(s) 24, 38
BtgI CCRYGG 1 cut(s) 1125
BtgZI GCGATG 1 cut(s) 311
BtsCI GGATG 2 cut(s) 748, 835
BtsI GCAGTG 3 cut(s) 73, 424, 918
BtsIMutI CAGTG 5 cut(s) 73, 182, 424, 453, 918
BveI ACCTGC 1 cut(s) 613
Cac8I GCNNGC 4 cut(s) 36, 225, 705, 723
CaiI CAGNNNCTG 1 cut(s) 907
CfoI GCGC 1 cut(s) 72
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 1 cut(s) 1264
CsiI ACCWGGT 1 cut(s) 1251
Csp6I GTAC 4 cut(s) 217, 447, 459, 1204
CviAII CATG 7 cut(s) 331, 395, 981, 1006, 1126, 1208, 1286
CviQI GTAC 4 cut(s) 217, 447, 459, 1204
DdeI CTNAG 1 cut(s) 895
DpnI GATC 7 cut(s) 60, 335, 374, 852, 969, 1176, 1284
DpnII GATC 7 cut(s) 58, 333, 372, 850, 967, 1174, 1282
DraIII CACNNNGTG 1 cut(s) 542
EaeI YGGCCR 1 cut(s) 36
EagI CGGCCG 1 cut(s) 36
Eam1104I CTCTTC 2 cut(s) 207, 846
EarI CTCTTC 2 cut(s) 207, 846
EciI GGCGGA 1 cut(s) 100
EclXI CGGCCG 1 cut(s) 36
Eco130I CCWWGG 3 cut(s) 150, 244, 1125
Eco31I GGTCTC 1 cut(s) 995
Eco47I GGWCC 1 cut(s) 1264
Eco52I CGGCCG 1 cut(s) 36
Eco72I CACGTG 1 cut(s) 683
EcoRII CCWGG 3 cut(s) 562, 648, 1251
EcoT14I CCWWGG 3 cut(s) 150, 244, 1125
EcoT22I ATGCAT 1 cut(s) 1297
ErhI CCWWGG 3 cut(s) 150, 244, 1125
Esp3I CGTCTC 2 cut(s) 106, 1280
FaeI CATG 7 cut(s) 334, 398, 984, 1009, 1129, 1211, 1289
FalI AAGNNNNNCTT 2 cut(s) 825, 857
FatI CATG 7 cut(s) 330, 394, 980, 1005, 1125, 1207, 1285
FbaI TGATCA 2 cut(s) 58, 1282
Fnu4HI GCNGC 6 cut(s) 348, 519, 629, 632, 837, 947
FokI GGATG 2 cut(s) 755, 842
Fsp4HI GCNGC 6 cut(s) 348, 519, 629, 632, 837, 947
FspBI CTAG 2 cut(s) 450, 722
GlaI GCGC 1 cut(s) 71
GluI GCNGC 6 cut(s) 348, 519, 629, 632, 837, 947
GsuI CTGGAG 2 cut(s) 585, 1049
HaeII RGCGCY 1 cut(s) 73
HaeIII GGCC 2 cut(s) 24, 38
HapII CCGG 1 cut(s) 35
HhaI GCGC 1 cut(s) 72
Hin1II CATG 7 cut(s) 334, 398, 984, 1009, 1129, 1211, 1289
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HindIII AAGCTT 1 cut(s) 1119
HinfI GANTC 3 cut(s) 752, 904, 1064
HpaII CCGG 1 cut(s) 35
HphI GGTGA 2 cut(s) 16, 398
Hpy188I TCNGA 2 cut(s) 372, 550
Hpy188III TCNNGA 9 cut(s) 86, 257, 762, 791, 845, 1028, 1046, 1103, 1157
HpyAV CCTTC 3 cut(s) 124, 1052, 1088
HpyCH4III ACNGT 1 cut(s) 186
HpyCH4IV ACGT 3 cut(s) 682, 891, 1273
HpyF10VI GCNNNNNNNGC 4 cut(s) 347, 625, 922, 1127
HpyF3I CTNAG 1 cut(s) 895
HpySE526I ACGT 3 cut(s) 682, 891, 1273
Hsp92II CATG 7 cut(s) 334, 398, 984, 1009, 1129, 1211, 1289
HspAI GCGC 1 cut(s) 70
KroI GCCGGC 1 cut(s) 34
KroNI GCCGGC 1 cut(s) 36
Ksp22I TGATCA 2 cut(s) 58, 1282
Kzo9I GATC 7 cut(s) 58, 333, 372, 850, 967, 1174, 1282
LguI GCTCTTC 1 cut(s) 846
LmnI GCTCC 2 cut(s) 67, 88
Lsp1109I GCAGC 6 cut(s) 359, 530, 615, 643, 848, 958
LweI GCATC 4 cut(s) 334, 516, 718, 958
MabI ACCWGGT 1 cut(s) 1251
MaeI CTAG 2 cut(s) 450, 722
MaeII ACGT 3 cut(s) 682, 891, 1273
MaeIII GTNAC 3 cut(s) 426, 713, 1144
MalI GATC 7 cut(s) 60, 335, 374, 852, 969, 1176, 1284
MboI GATC 7 cut(s) 58, 333, 372, 850, 967, 1174, 1282
MboII GAAGA 9 cut(s) 224, 304, 413, 779, 833, 977, 1091, 1127, 1145
MfeI CAATTG 3 cut(s) 281, 583, 1191
MflI RGATCY 1 cut(s) 967
MluCI AATT 9 cut(s) 281, 405, 583, 597, 810, 872, 1016, 1191, 1223
MlyI GAGTC 2 cut(s) 746, 1073
MmeI TCCRAC 3 cut(s) 45, 151, 501
Mph1103I ATGCAT 1 cut(s) 1297
MroNI GCCGGC 1 cut(s) 34
MroXI GAANNNNTTC 1 cut(s) 903
MseI TTAA 2 cut(s) 806, 921
MslI CAYNNNNRTG 3 cut(s) 960, 985, 1290
MspA1I CMGCKG 2 cut(s) 307, 628
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 3 cut(s) 564, 650, 1253
MunI CAATTG 3 cut(s) 281, 583, 1191
MvaI CCWGG 3 cut(s) 564, 650, 1253
MwoI GCNNNNNNNGC 4 cut(s) 347, 625, 922, 1127
NaeI GCCGGC 1 cut(s) 36
NcoI CCATGG 1 cut(s) 1125
NdeII GATC 7 cut(s) 58, 333, 372, 850, 967, 1174, 1282
NgoMIV GCCGGC 1 cut(s) 34
NheI GCTAGC 1 cut(s) 721
NlaIII CATG 7 cut(s) 334, 398, 984, 1009, 1129, 1211, 1289
NlaIV GGNNCC 3 cut(s) 84, 94, 561
NmuCI GTSAC 2 cut(s) 426, 713
NsiI ATGCAT 1 cut(s) 1297
PaqCI CACCTGC 1 cut(s) 613
PciSI GCTCTTC 1 cut(s) 846
PdiI GCCGGC 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 903
PfeI GAWTC 1 cut(s) 904
PflMI CCANNNNNTGG 1 cut(s) 79
PkrI GCNGC 6 cut(s) 349, 520, 630, 633, 838, 948
PleI GAGTC 2 cut(s) 746, 1072
PmaCI CACGTG 1 cut(s) 683
PmlI CACGTG 1 cut(s) 683
PpsI GAGTC 2 cut(s) 746, 1072
Ppu21I YACGTR 1 cut(s) 683
PshAI GACNNNNGTC 1 cut(s) 50
PsiI TTATAA 3 cut(s) 737, 888, 1134
Psp6I CCWGG 3 cut(s) 562, 648, 1251
PspCI CACGTG 1 cut(s) 683
PspGI CCWGG 3 cut(s) 562, 648, 1251
PspN4I GGNNCC 3 cut(s) 84, 94, 561
PspPI GGNCC 1 cut(s) 1264
PstI CTGCAG 2 cut(s) 633, 865
PstNI CAGNNNCTG 1 cut(s) 907
PsuI RGATCY 1 cut(s) 967
PvuII CAGCTG 2 cut(s) 307, 628
RsaI GTAC 4 cut(s) 218, 448, 460, 1205
RsaNI GTAC 4 cut(s) 217, 447, 459, 1204
RseI CAYNNNNRTG 3 cut(s) 960, 985, 1290
SapI GCTCTTC 1 cut(s) 846
SaqAI TTAA 2 cut(s) 806, 921
SatI GCNGC 6 cut(s) 348, 519, 629, 632, 837, 947
Sau3AI GATC 7 cut(s) 58, 333, 372, 850, 967, 1174, 1282
Sau96I GGNCC 1 cut(s) 1264
ScaI AGTACT 1 cut(s) 448
SchI GAGTC 2 cut(s) 746, 1073
ScrFI CCNGG 3 cut(s) 564, 650, 1253
SexAI ACCWGGT 1 cut(s) 1251
SfaNI GCATC 4 cut(s) 334, 516, 718, 958
SfcI CTRYAG 3 cut(s) 629, 861, 1110
SinI GGWCC 1 cut(s) 1264
SmiMI CAYNNNNRTG 3 cut(s) 960, 985, 1290
SmlI CTYRAG 1 cut(s) 469
SmoI CTYRAG 1 cut(s) 469
Sse9I AATT 9 cut(s) 281, 405, 583, 597, 810, 872, 1016, 1191, 1223
SsiI CCGC 3 cut(s) 111, 227, 1142
SspI AATATT 1 cut(s) 884
SspMI CTAG 2 cut(s) 450, 722
StyD4I CCNGG 3 cut(s) 562, 648, 1251
StyI CCWWGG 3 cut(s) 150, 244, 1125
TaaI ACNGT 1 cut(s) 186
TaiI ACGT 3 cut(s) 685, 894, 1276
TaqI TCGA 5 cut(s) 233, 256, 482, 1156, 1185
TasI AATT 9 cut(s) 281, 405, 583, 597, 810, 872, 1016, 1191, 1223
TatI WGTACW 1 cut(s) 446
TfiI GAWTC 1 cut(s) 904
Tru1I TTAA 2 cut(s) 806, 921
Tru9I TTAA 2 cut(s) 806, 921
TscAI CASTG 5 cut(s) 80, 189, 424, 460, 918
TseFI GTSAC 2 cut(s) 426, 713
TseI GCWGC 6 cut(s) 347, 518, 628, 631, 836, 946
Tsp45I GTSAC 2 cut(s) 426, 713
TspDTI ATGAA 3 cut(s) 846, 978, 1070
TspGWI ACGGA 1 cut(s) 445
TspRI CASTG 5 cut(s) 80, 189, 424, 460, 918
Van91I CCANNNNNTGG 1 cut(s) 79
VpaK11BI GGWCC 1 cut(s) 1264
XapI RAATTY 1 cut(s) 872
XmnI GAANNNNTTC 1 cut(s) 903
XspI CTAG 2 cut(s) 450, 722
ZrmI AGTACT 1 cut(s) 448
Zsp2I ATGCAT 1 cut(s) 1297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.