MD17G1190200.v1.1

HAUS augmin-like complex subunit 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002315
Physical Location & Seq
Reverse (-)
22668377 .. 22673481
5105 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1190200.v1.1.491

Sequence Viewer

Length: 1293 bp
ATGGCGAAGGGACTGCAACAACAACAAGGGCAAAACCTGCCATCGGATTTGACCCAAGTCATTGATCAGCTGGAGCGCCACTGCTTGGCTCCCGATGGTTCTCTCGTCTCCAAATCTGCCTACTTCGACCTCCAACTCGCAAGGGAGGAAATGTCCCGGGAAAGATTGCGATACTTGGAAGCCATGGCATTGTATAGTGAGGCAATTGCAATGGTGGAGGAGTATCAACAAGCGGTTTCTGTGGCTAACCTTGGAGGCATTCGGGATCTACAATTGGGCTTGAAGAACCCTCCTCAGTTGTATGAGACATTAGAGCATCGTATGATCGTTTCAGAAGCAGCTCAAAGACTGAGGCTTCCTCTTATCTCCAAAGATGGTGAAATTCACGAGGAAGAAATTGAAAAGTGTAGTACCATGTCACGCAGTTCTCTAGAAAGTACCAGTACCAGTGTTACGGTCAGCTCAAGCTCCAACTCAACAAATTATAATGCTGTGACTAGCACTGCTAGTGGCGCAAACAACAATATTTCTCTTAGTGCAACTGAAACTGTGGAATCTGGGGTTGGTGGTATTCCCAATTGCTTTCTTGGAATAACACCTGCATATTTATGGCAAACACAGCTCCTACAAACACCGTTGTCAATGGATATGACAGAATACCAGTTGTGTCTTTCCCAAGAGATTGAAGCTCGTTTAAAAGCTAAATGTGATAAGCTAGCTGATGCCTTTATAATGGATGACATTGACTCGTCATCTGGGCATCAAAATTCAAGTTCTCGGCTTCCAGAAAGGGTAAAGTTGATAATTGAGGAGATGGAAAGGGAAGAAACAGCTCTGCGGGAAGATCTATATTCCGCAGATAGAAAATTTGCTGAATATTATAATGTCCTGGAGCAGATACTGGGAGTGCTTATCAAGCTGGTCAGAGATTTGAAGTTGCAACATCAACATAAATATGACGGACTGCAAAAAACATGGCTGTGCAAAAGGTGTGAGACCATGAGTGCAAAATTGAGGGTCCTAGAGCATGTTCTCCTTCTGGAAACTTATACTAAGGAATCAATACCGGCCCTACATAAAATAAGGAAGTATCTCCTTGAAGCTACAGAGGAAGCTTCTATTGCATATAATAAAGCGGTTACACGTCTTCGGGAGTATCAGGGTGTTGATCCTCACTTTGATACAATTGCAAGGCAGTACCACGATATTATAAAGAAATTGGAAAATATGCAGTGGACAATCCACCAAGTTGAAATGGACTTGAAGCGACTCCCAGATCACGCAAATGCATAG

Protein Analysis

431

Amino Acids

48.82

Weight (kDa)

5.37

Isoelectric Point (pI)

48.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS4 PF14735 184 - 419 2.5e-82 HAUS augmin-like complex subunit 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 486, 731, 882, 1211
AarI CACCTGC 1 cut(s) 607
Acc36I ACCTGC 2 cut(s) 45, 607
AccB7I CCANNNNNTGG 1 cut(s) 85
AciI CCGC 4 cut(s) 233, 838, 855, 1136
AclWI GGATC 2 cut(s) 273, 1163
AcsI RAATTY 3 cut(s) 381, 766, 866
AfaI GTAC 4 cut(s) 412, 439, 445, 1199
AfiI CCNNNNNNNGG 2 cut(s) 43, 85
AflIII ACRYGT 1 cut(s) 1142
AgsI TTSAA 8 cut(s) 283, 401, 686, 771, 934, 1100, 1253, 1264
AjiI CACGTC 1 cut(s) 1145
AjnI CCWGG 1 cut(s) 888
AjuI GAANNNNNNNTTGG 2 cut(s) 546, 578
Alw26I GTCTC 3 cut(s) 112, 299, 989
AlwI GGATC 2 cut(s) 273, 1163
AlwNI CAGNNNCTG 1 cut(s) 901
Ama87I CYCGRG 1 cut(s) 156
AoxI GGCC 1 cut(s) 1068
ApeKI GCWGC 1 cut(s) 338
ApoI RAATTY 3 cut(s) 381, 766, 866
AspLEI GCGC 2 cut(s) 78, 515
AspS9I GGNCC 2 cut(s) 1018, 1069
AsuC2I CCSGG 2 cut(s) 157, 158
AsuHPI GGTGA 1 cut(s) 389
AsuNHI GCTAGC 1 cut(s) 715
AvaI CYCGRG 1 cut(s) 156
AvaII GGWCC 1 cut(s) 1018
BauI CACGAG 1 cut(s) 386
BbsI GAAGAC 1 cut(s) 1139
BbvI GCAGC 1 cut(s) 350
BccI CCATC 4 cut(s) 49, 89, 368, 808
BcgI CGANNNNNNTGC 1 cut(s) 29
BciT130I CCWGG 1 cut(s) 890
BclI TGATCA 1 cut(s) 64
BcnI CCSGG 2 cut(s) 157, 158
BcoDI GTCTC 3 cut(s) 112, 299, 989
BfaI CTAG 5 cut(s) 431, 498, 507, 716, 1022
BfmI CTRYAG 1 cut(s) 1104
BfoI RGCGCY 1 cut(s) 79
BfuAI ACCTGC 2 cut(s) 45, 607
BglII AGATCT 1 cut(s) 844
BisI GCNGC 1 cut(s) 339
BlsI GCNGC 1 cut(s) 340
Bme1390I CCNGG 3 cut(s) 157, 158, 890
Bme18I GGWCC 1 cut(s) 1018
BmeT110I CYCGRG 1 cut(s) 156
BmgBI CACGTC 1 cut(s) 1145
BmgT120I GGNCC 2 cut(s) 1018, 1069
BmiI GGNNCC 2 cut(s) 90, 1019
BmrFI CCNGG 3 cut(s) 157, 158, 890
BmrI ACTGGG 1 cut(s) 911
BmsI GCATC 3 cut(s) 325, 712, 769
BmtI GCTAGC 1 cut(s) 719
BmuI ACTGGG 1 cut(s) 911
BoxI GACNNNNGTC 1 cut(s) 56
BpiI GAAGAC 1 cut(s) 1139
BplI GAGNNNNNCTC 2 cut(s) 343, 375
BpmI CTGGAG 2 cut(s) 92, 911
BpuEI CTTGAG 1 cut(s) 448
BpuMI CCSGG 2 cut(s) 157, 158
BsaI GGTCTC 1 cut(s) 989
BsaJI CCNNGG 3 cut(s) 156, 183, 250
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 85
Bse118I RCCGGY 1 cut(s) 1066
Bse1I ACTGG 4 cut(s) 441, 447, 661, 906
Bse3DI GCAATG 1 cut(s) 216
BseBI CCWGG 1 cut(s) 890
BseDI CCNNGG 3 cut(s) 156, 183, 250
BseGI GGATG 1 cut(s) 742
BseLI CCNNNNNNNGG 2 cut(s) 43, 85
BseMI GCAATG 1 cut(s) 216
BseMII CTCAG 2 cut(s) 308, 341
BseNI ACTGG 4 cut(s) 441, 447, 661, 906
BseRI GAGGAG 3 cut(s) 233, 282, 824
BseXI GCAGC 1 cut(s) 350
BshFI GGCC 1 cut(s) 1070
BsiHKCI CYCGRG 1 cut(s) 156
BsiSI CCGG 2 cut(s) 157, 1067
BslFI GGGAC 2 cut(s) 24, 139
BslI CCNNNNNNNGG 2 cut(s) 43, 85
BsmAI GTCTC 3 cut(s) 112, 299, 989
BsmBI CGTCTC 1 cut(s) 112
BsmFI GGGAC 2 cut(s) 24, 139
BsmI GAATGC 1 cut(s) 258
BsnI GGCC 1 cut(s) 1070
Bso31I GGTCTC 1 cut(s) 989
BsoBI CYCGRG 1 cut(s) 156
Bsp143I GATC 6 cut(s) 64, 265, 324, 844, 1168, 1276
Bsp19I CCATGG 1 cut(s) 183
BspACI CCGC 4 cut(s) 233, 838, 855, 1136
BspANI GGCC 1 cut(s) 1070
BspCNI CTCAG 2 cut(s) 307, 342
BspLI GGNNCC 2 cut(s) 90, 1019
BspMI ACCTGC 2 cut(s) 45, 607
BspOI GCTAGC 1 cut(s) 719
BspPI GGATC 2 cut(s) 273, 1163
BspTNI GGTCTC 1 cut(s) 989
BsrDI GCAATG 1 cut(s) 216
BsrFI RCCGGY 1 cut(s) 1066
BsrI ACTGG 4 cut(s) 441, 447, 661, 906
BssAI RCCGGY 1 cut(s) 1066
BssECI CCNNGG 3 cut(s) 156, 183, 250
BssMI GATC 6 cut(s) 64, 265, 324, 844, 1168, 1276
BssSI CACGAG 1 cut(s) 386
BssT1I CCWWGG 2 cut(s) 183, 250
Bst2BI CACGAG 1 cut(s) 386
Bst2UI CCWGG 1 cut(s) 890
Bst4CI ACNGT 3 cut(s) 457, 550, 636
BstAPI GCANNNNNTGC 1 cut(s) 37
BstC8I GCNNGC 1 cut(s) 717
BstDEI CTNAG 4 cut(s) 294, 350, 533, 1053
BstDSI CCRYGG 1 cut(s) 183
BstF5I GGATG 1 cut(s) 742
BstH2I RGCGCY 1 cut(s) 79
BstHHI GCGC 2 cut(s) 78, 515
BstKTI GATC 6 cut(s) 67, 268, 327, 847, 1171, 1279
BstMAI GTCTC 3 cut(s) 112, 299, 989
BstMBI GATC 6 cut(s) 64, 265, 324, 844, 1168, 1276
BstMWI GCNNNNNNNGC 5 cut(s) 37, 512, 619, 916, 1121
BstNI CCWGG 1 cut(s) 890
BstNSI RCATGY 1 cut(s) 1031
BstPAI GACNNNNGTC 1 cut(s) 56
BstSCI CCNGG 3 cut(s) 155, 156, 888
BstSFI CTRYAG 1 cut(s) 1104
BstV1I GCAGC 1 cut(s) 350
BstV2I GAAGAC 1 cut(s) 1139
BstX2I RGATCY 2 cut(s) 265, 844
BstYI RGATCY 2 cut(s) 265, 844
BsuRI GGCC 1 cut(s) 1070
BtgI CCRYGG 1 cut(s) 183
BtrI CACGTC 1 cut(s) 1145
BtsCI GGATG 1 cut(s) 742
BtsI GCAGTG 3 cut(s) 79, 501, 1238
BtsIMutI CAGTG 4 cut(s) 79, 454, 501, 1238
BveI ACCTGC 2 cut(s) 45, 607
Cac8I GCNNGC 1 cut(s) 717
CaiI CAGNNNCTG 1 cut(s) 901
CfoI GCGC 2 cut(s) 78, 515
Cfr10I RCCGGY 1 cut(s) 1066
Cfr13I GGNCC 2 cut(s) 1018, 1069
Cfr9I CCCGGG 1 cut(s) 156
Csp6I GTAC 4 cut(s) 411, 438, 444, 1198
CviAII CATG 5 cut(s) 184, 415, 975, 1000, 1028
CviQI GTAC 4 cut(s) 411, 438, 444, 1198
DdeI CTNAG 4 cut(s) 294, 350, 533, 1053
DpnI GATC 6 cut(s) 66, 267, 326, 846, 1170, 1278
DpnII GATC 6 cut(s) 64, 265, 324, 844, 1168, 1276
DraI TTTAAA 1 cut(s) 696
Eco130I CCWWGG 2 cut(s) 183, 250
Eco31I GGTCTC 1 cut(s) 989
Eco47I GGWCC 1 cut(s) 1018
Eco88I CYCGRG 1 cut(s) 156
EcoO109I RGGNCCY 1 cut(s) 1018
EcoRII CCWGG 1 cut(s) 888
EcoT14I CCWWGG 2 cut(s) 183, 250
EcoT22I ATGCAT 1 cut(s) 1291
ErhI CCWWGG 2 cut(s) 183, 250
Esp3I CGTCTC 1 cut(s) 112
FaeI CATG 5 cut(s) 187, 418, 978, 1003, 1031
FaqI GGGAC 2 cut(s) 24, 139
FatI CATG 5 cut(s) 183, 414, 974, 999, 1027
FauI CCCGC 1 cut(s) 831
FbaI TGATCA 1 cut(s) 64
Fnu4HI GCNGC 1 cut(s) 339
FokI GGATG 1 cut(s) 749
Fsp4HI GCNGC 1 cut(s) 339
FspBI CTAG 5 cut(s) 431, 498, 507, 716, 1022
GlaI GCGC 2 cut(s) 77, 514
GluI GCNGC 1 cut(s) 339
GsuI CTGGAG 2 cut(s) 92, 911
HaeII RGCGCY 1 cut(s) 79
HaeIII GGCC 1 cut(s) 1070
HapII CCGG 2 cut(s) 157, 1067
HhaI GCGC 2 cut(s) 78, 515
Hin1II CATG 5 cut(s) 187, 418, 978, 1003, 1031
Hin6I GCGC 2 cut(s) 76, 513
HinP1I GCGC 2 cut(s) 76, 513
HindIII AAGCTT 1 cut(s) 1113
HinfI GANTC 4 cut(s) 554, 746, 1058, 1269
HpaII CCGG 2 cut(s) 157, 1067
HphI GGTGA 1 cut(s) 389
Hpy166II GTNNAC 1 cut(s) 1236
Hpy188I TCNGA 3 cut(s) 46, 334, 926
Hpy188III TCNNGA 7 cut(s) 92, 263, 386, 431, 785, 1040, 1151
Hpy8I GTNNAC 1 cut(s) 1236
HpyAV CCTTC 1 cut(s) 1046
HpyCH4III ACNGT 3 cut(s) 457, 550, 636
HpyCH4IV ACGT 1 cut(s) 1144
HpyF10VI GCNNNNNNNGC 5 cut(s) 37, 512, 619, 916, 1121
HpyF3I CTNAG 4 cut(s) 294, 350, 533, 1053
HpySE526I ACGT 1 cut(s) 1144
Hsp92II CATG 5 cut(s) 187, 418, 978, 1003, 1031
HspAI GCGC 2 cut(s) 76, 513
Ksp22I TGATCA 1 cut(s) 64
Kzo9I GATC 6 cut(s) 64, 265, 324, 844, 1168, 1276
LmnI GCTCC 5 cut(s) 73, 94, 473, 627, 892
Lsp1109I GCAGC 1 cut(s) 350
LweI GCATC 3 cut(s) 325, 712, 769
MaeI CTAG 5 cut(s) 431, 498, 507, 716, 1022
MaeII ACGT 1 cut(s) 1144
MaeIII GTNAC 4 cut(s) 417, 451, 493, 1138
MalI GATC 6 cut(s) 66, 267, 326, 846, 1170, 1278
MboI GATC 6 cut(s) 64, 265, 324, 844, 1168, 1276
MboII GAAGA 5 cut(s) 295, 404, 836, 854, 1139
MfeI CAATTG 4 cut(s) 204, 272, 577, 1185
MflI RGATCY 2 cut(s) 265, 844
MlyI GAGTC 2 cut(s) 740, 1263
MmeI TCCRAC 2 cut(s) 157, 495
Mph1103I ATGCAT 1 cut(s) 1291
MseI TTAA 1 cut(s) 695
MslI CAYNNNNRTG 4 cut(s) 607, 954, 979, 1284
MspA1I CMGCKG 1 cut(s) 70
MspI CCGG 2 cut(s) 157, 1067
MspR9I CCNGG 3 cut(s) 157, 158, 890
MunI CAATTG 4 cut(s) 204, 272, 577, 1185
Mva1269I GAATGC 1 cut(s) 258
MvaI CCWGG 1 cut(s) 890
MwoI GCNNNNNNNGC 5 cut(s) 37, 512, 619, 916, 1121
NciI CCSGG 2 cut(s) 157, 158
NcoI CCATGG 1 cut(s) 183
NdeII GATC 6 cut(s) 64, 265, 324, 844, 1168, 1276
NheI GCTAGC 1 cut(s) 715
NlaIII CATG 5 cut(s) 187, 418, 978, 1003, 1031
NlaIV GGNNCC 2 cut(s) 90, 1019
NmeAIII GCCGAG 1 cut(s) 757
NmuCI GTSAC 2 cut(s) 417, 493
NsiI ATGCAT 1 cut(s) 1291
NspI RCATGY 1 cut(s) 1031
PaqCI CACCTGC 1 cut(s) 607
PctI GAATGC 1 cut(s) 258
PfeI GAWTC 2 cut(s) 554, 1058
PflMI CCANNNNNTGG 1 cut(s) 85
PfoI TCCNGGA 1 cut(s) 888
PkrI GCNGC 1 cut(s) 340
PleI GAGTC 2 cut(s) 740, 1263
PpsI GAGTC 2 cut(s) 740, 1263
PpuMI RGGWCCY 1 cut(s) 1018
PshAI GACNNNNGTC 1 cut(s) 56
PsiI TTATAA 4 cut(s) 486, 731, 882, 1211
Psp5II RGGWCCY 1 cut(s) 1018
Psp6I CCWGG 1 cut(s) 888
PspGI CCWGG 1 cut(s) 888
PspN4I GGNNCC 2 cut(s) 90, 1019
PspPI GGNCC 2 cut(s) 1018, 1069
PspPPI RGGWCCY 1 cut(s) 1018
PstNI CAGNNNCTG 1 cut(s) 901
PsuI RGATCY 2 cut(s) 265, 844
PvuII CAGCTG 1 cut(s) 70
RsaI GTAC 4 cut(s) 412, 439, 445, 1199
RsaNI GTAC 4 cut(s) 411, 438, 444, 1198
RseI CAYNNNNRTG 4 cut(s) 607, 954, 979, 1284
SaqAI TTAA 1 cut(s) 695
SatI GCNGC 1 cut(s) 339
Sau3AI GATC 6 cut(s) 64, 265, 324, 844, 1168, 1276
Sau96I GGNCC 2 cut(s) 1018, 1069
SchI GAGTC 2 cut(s) 740, 1263
ScrFI CCNGG 3 cut(s) 157, 158, 890
SfaNI GCATC 3 cut(s) 325, 712, 769
SfcI CTRYAG 1 cut(s) 1104
SinI GGWCC 1 cut(s) 1018
SmaI CCCGGG 1 cut(s) 158
SmiMI CAYNNNNRTG 4 cut(s) 607, 954, 979, 1284
SmlI CTYRAG 1 cut(s) 463
SmoI CTYRAG 1 cut(s) 463
SsiI CCGC 4 cut(s) 233, 838, 855, 1136
SspI AATATT 2 cut(s) 526, 878
SspMI CTAG 5 cut(s) 431, 498, 507, 716, 1022
StyD4I CCNGG 3 cut(s) 155, 156, 888
StyI CCWWGG 2 cut(s) 183, 250
TaaI ACNGT 3 cut(s) 457, 550, 636
TaiI ACGT 1 cut(s) 1147
TaqI TCGA 1 cut(s) 126
TfiI GAWTC 2 cut(s) 554, 1058
Tru1I TTAA 1 cut(s) 695
Tru9I TTAA 1 cut(s) 695
TscAI CASTG 4 cut(s) 86, 454, 508, 1238
TseFI GTSAC 2 cut(s) 417, 493
TseI GCWGC 1 cut(s) 338
Tsp45I GTSAC 2 cut(s) 417, 493
TspGWI ACGGA 1 cut(s) 975
TspMI CCCGGG 1 cut(s) 156
TspRI CASTG 4 cut(s) 86, 454, 508, 1238
Van91I CCANNNNNTGG 1 cut(s) 85
VpaK11BI GGWCC 1 cut(s) 1018
XapI RAATTY 3 cut(s) 381, 766, 866
XbaI TCTAGA 1 cut(s) 430
XceI RCATGY 1 cut(s) 1031
XmaI CCCGGG 1 cut(s) 156
XspI CTAG 5 cut(s) 431, 498, 507, 716, 1022
Zsp2I ATGCAT 1 cut(s) 1291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.