Rh5BG272800

HAUS augmin-like complex subunit 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002315
Physical Location & Seq
Reverse (-)
34237733 .. 34241411
3679 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG272800.1

Sequence Viewer

Length: 1242 bp
ATGTTGAAGAGACTGCAATTACAAGAACAATGCCAGCTACAGGATTTGAGCCAAGTCATTGATCATCTGGGGCGCCACTGCTTCGCTCCCGATGATTCTCTCGTCTCCAAATCCGCATACTACGACCTCCAACTCGCTAGGGAGGAAATGCGCAGGGAAAGACTGCGTTACTTGGAAGCCATGGCAATTTATAGTGAGGCAATTGCAATGGTGGAAGATTACCACCAAGGCTGGGGGAACCCTCCTCAGCTGTTTGAGACGTTGGAGCATCTTATGATCACTGCAGAAGCCGCTCAAAGGTTGAGGCTTCCTCTGCTCTCCAAAGACGGTGATATTCATGAGGAAGAAATTGAAAGATGCAGCATAGTGTCACAAAGTTCCCTCGATAGTACTTGTACCAGTTTCACAGTCGGGTCAAGTTCTAACTCTCCAAATTATAGTGGTGCAAATAGCACAGTTAGTGCAGCAAACAACAATCTTTGTCTCAGTGCTACTTCAGTCGAGGAACCTGGACTTGGAGGCGTTCCCAATCGCTTTCTTGGAATTACACCTGCATTTTTATGGAAAACACAGCTTCAACAAATCCCTTTCGACATGGATCTGGCAGAATACCGGCTGTGTCTTTTCGATGAGATTGAGGCTCGTTTGCAAGCTAAATGTGATAAGTTAGCCGACCCCTTTGTAATGGAAGACATAGACTGGTCGTCATCCGGACAGGAAAATACAAGTGATCAGTTACCAGAAAGGGTAAAGTCAATAACTGAGGAGCTGGATAGGGAGGAAGCAGCTCTGAAGGAAGATCTGTATTCAGCAGAAAGAGGATTTACTGAATACTACAATGTTTTAGAGCAGATATTGGGAGTTCTTATAAAGCTGGTCAGAAATTTGAAGTTGCAACATCAACACAAATATGATGAACTGAAAAAGATATTCTTGTGCAAAAGGTGCGAGACCATAGATGCAAAATTGAGGAGCCTGGAGTATGGTCTCCTTCGGGACACTTATTCCAAGGATTCAGTACCTGCCCTGCATAAAATAAGGAAATATCTTCTTGAAGCTACAAAAGAAGCTTCAATCACACATGATAAAGCAGTTACTCGCCTTCATGAGTATCAGAGTGTTGATGCTCACTTCAATAAAATTGCAGGGCAGTACCGTGATATTTTAAAGAAGTTGGAAAATATGCAATGGACGATCCAGCAAGTAGAAATGGACCTGAAACGCGTCCCAGAACTACCCTAA

Protein Analysis

413

Amino Acids

47.42

Weight (kDa)

5.15

Isoelectric Point (pI)

50.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS4 PF14735 169 - 404 3.2e-72 HAUS augmin-like complex subunit 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 869
AarI CACCTGC 1 cut(s) 559
Acc16I TGCGCA 1 cut(s) 152
Acc36I ACCTGC 2 cut(s) 559, 1030
AccB1I GGYRCC 1 cut(s) 72
AccBSI CCGCTC 1 cut(s) 293
AccII CGCG 1 cut(s) 1224
AccIII TCCGGA 1 cut(s) 710
AciI CCGC 2 cut(s) 114, 291
AclWI GGATC 2 cut(s) 606, 1189
AcsI RAATTY 1 cut(s) 883
AcuI CTGAAG 2 cut(s) 480, 812
AcyI GRCGYC 1 cut(s) 73
AfaI GTAC 4 cut(s) 391, 397, 1020, 1154
AfiI CCNNNNNNNGG 4 cut(s) 40, 232, 297, 515
AflIII ACRYGT 1 cut(s) 1222
AgsI TTSAA 7 cut(s) 7, 353, 578, 889, 1055, 1074, 1135
AhdI GACNNNNNGTC 1 cut(s) 703
AjnI CCWGG 2 cut(s) 508, 975
AjuI GAANNNNNNNTTGG 2 cut(s) 498, 530
AluBI AGCT 9 cut(s) 37, 250, 574, 653, 769, 788, 874, 1058, 1070
AluI AGCT 9 cut(s) 37, 250, 574, 653, 769, 788, 874, 1058, 1070
Alw26I GTCTC 6 cut(s) 4, 109, 251, 488, 944, 992
AlwI GGATC 2 cut(s) 606, 1189
AlwNI CAGNNNCTG 1 cut(s) 1022
Aor13HI TCCGGA 1 cut(s) 710
ApeKI GCWGC 3 cut(s) 360, 464, 785
ApoI RAATTY 1 cut(s) 883
AspLEI GCGC 2 cut(s) 75, 153
AspS9I GGNCC 1 cut(s) 1213
AsuHPI GGTGA 1 cut(s) 341
AvaII GGWCC 1 cut(s) 1213
BanI GGYRCC 1 cut(s) 72
BbsI GAAGAC 1 cut(s) 696
BbvCI CCTCAGC 1 cut(s) 246
BbvI GCAGC 3 cut(s) 372, 476, 797
BciT130I CCWGG 2 cut(s) 510, 977
BclI TGATCA 3 cut(s) 61, 276, 730
BcoDI GTCTC 6 cut(s) 4, 109, 251, 488, 944, 992
BfaI CTAG 1 cut(s) 138
BfmI CTRYAG 2 cut(s) 38, 282
BfoI RGCGCY 1 cut(s) 76
BfuAI ACCTGC 2 cut(s) 559, 1030
BglII AGATCT 1 cut(s) 799
BisI GCNGC 4 cut(s) 291, 361, 465, 786
BlsI GCNGC 4 cut(s) 292, 362, 466, 787
BmcAI AGTACT 1 cut(s) 391
Bme1390I CCNGG 2 cut(s) 510, 977
Bme18I GGWCC 1 cut(s) 1213
BmeRI GACNNNNNGTC 1 cut(s) 703
BmgT120I GGNCC 1 cut(s) 1213
BmiI GGNNCC 4 cut(s) 74, 239, 507, 974
BmrFI CCNGG 2 cut(s) 510, 977
BmsI GCATC 4 cut(s) 277, 347, 949, 1114
BpiI GAAGAC 1 cut(s) 696
BplI GAGNNNNNCTC 2 cut(s) 295, 327
BpmI CTGGAG 1 cut(s) 998
Bpu10I CCTNAGC 1 cut(s) 246
BsaHI GRCGYC 1 cut(s) 73
BsaI GGTCTC 2 cut(s) 944, 992
BsaJI CCNNGG 3 cut(s) 180, 226, 1008
BsaWI WCCGGW 1 cut(s) 710
Bsc4I CCNNNNNNNGG 4 cut(s) 40, 232, 297, 515
Bse118I RCCGGY 1 cut(s) 612
Bse1I ACTGG 2 cut(s) 399, 704
Bse3DI GCAATG 2 cut(s) 213, 1193
BseAI TCCGGA 1 cut(s) 710
BseBI CCWGG 2 cut(s) 510, 977
BseDI CCNNGG 3 cut(s) 180, 226, 1008
BseGI GGATG 1 cut(s) 707
BseLI CCNNNNNNNGG 4 cut(s) 40, 232, 297, 515
BseMI GCAATG 2 cut(s) 213, 1193
BseMII CTCAG 3 cut(s) 260, 499, 753
BseNI ACTGG 2 cut(s) 399, 704
BseRI GAGGAG 3 cut(s) 234, 779, 985
BseXI GCAGC 3 cut(s) 372, 476, 797
BseYI CCCAGC 1 cut(s) 231
BsgI GTGCAG 1 cut(s) 483
Bsh1236I CGCG 1 cut(s) 1224
BshNI GGYRCC 1 cut(s) 72
BsiSI CCGG 2 cut(s) 613, 711
BslFI GGGAC 2 cut(s) 1010, 1211
BslI CCNNNNNNNGG 4 cut(s) 40, 232, 297, 515
BsmAI GTCTC 6 cut(s) 4, 109, 251, 488, 944, 992
BsmBI CGTCTC 2 cut(s) 109, 251
BsmFI GGGAC 2 cut(s) 1010, 1211
Bso31I GGTCTC 2 cut(s) 944, 992
Bsp13I TCCGGA 1 cut(s) 710
Bsp143I GATC 6 cut(s) 61, 276, 598, 730, 799, 1194
Bsp19I CCATGG 1 cut(s) 180
BspACI CCGC 2 cut(s) 114, 291
BspCNI CTCAG 3 cut(s) 259, 498, 754
BspEI TCCGGA 1 cut(s) 710
BspFNI CGCG 1 cut(s) 1224
BspHI TCATGA 2 cut(s) 337, 1105
BspLI GGNNCC 4 cut(s) 74, 239, 507, 974
BspMAI CTGCAG 1 cut(s) 286
BspMI ACCTGC 2 cut(s) 559, 1030
BspPI GGATC 2 cut(s) 606, 1189
BspT107I GGYRCC 1 cut(s) 72
BspTNI GGTCTC 2 cut(s) 944, 992
BsrBI CCGCTC 1 cut(s) 293
BsrDI GCAATG 2 cut(s) 213, 1193
BsrFI RCCGGY 1 cut(s) 612
BsrI ACTGG 2 cut(s) 399, 704
BssAI RCCGGY 1 cut(s) 612
BssECI CCNNGG 3 cut(s) 180, 226, 1008
BssMI GATC 6 cut(s) 61, 276, 598, 730, 799, 1194
BssNI GRCGYC 1 cut(s) 73
BssT1I CCWWGG 3 cut(s) 180, 226, 1008
Bst2UI CCWGG 2 cut(s) 510, 977
Bst4CI ACNGT 4 cut(s) 329, 409, 457, 1157
Bst6I CTCTTC 1 cut(s) 2
BstACI GRCGYC 1 cut(s) 73
BstAPI GCANNNNNTGC 1 cut(s) 945
BstC8I GCNNGC 2 cut(s) 35, 651
BstDEI CTNAG 3 cut(s) 246, 485, 762
BstDSI CCRYGG 1 cut(s) 180
BstF5I GGATG 1 cut(s) 707
BstFNI CGCG 1 cut(s) 1224
BstH2I RGCGCY 1 cut(s) 76
BstHHI GCGC 2 cut(s) 75, 153
BstKTI GATC 6 cut(s) 64, 279, 601, 733, 802, 1197
BstMAI GTCTC 6 cut(s) 4, 109, 251, 488, 944, 992
BstMBI GATC 6 cut(s) 61, 276, 598, 730, 799, 1194
BstMWI GCNNNNNNNGC 3 cut(s) 290, 313, 945
BstNI CCWGG 2 cut(s) 510, 977
BstSCI CCNGG 2 cut(s) 508, 975
BstSFI CTRYAG 2 cut(s) 38, 282
BstUI CGCG 1 cut(s) 1224
BstV1I GCAGC 3 cut(s) 372, 476, 797
BstV2I GAAGAC 1 cut(s) 696
BstX2I RGATCY 2 cut(s) 598, 799
BstYI RGATCY 2 cut(s) 598, 799
BtgI CCRYGG 1 cut(s) 180
BtsCI GGATG 1 cut(s) 707
BtsI GCAGTG 2 cut(s) 76, 279
BtsIMutI CAGTG 3 cut(s) 76, 279, 493
BveI ACCTGC 2 cut(s) 559, 1030
Cac8I GCNNGC 2 cut(s) 35, 651
CaiI CAGNNNCTG 1 cut(s) 1022
CciI TCATGA 2 cut(s) 337, 1105
CfoI GCGC 2 cut(s) 75, 153
Cfr10I RCCGGY 1 cut(s) 612
Cfr13I GGNCC 1 cut(s) 1213
CseI GACGC 1 cut(s) 1213
Csp6I GTAC 4 cut(s) 390, 396, 1019, 1153
CviAII CATG 5 cut(s) 181, 338, 595, 1082, 1106
CviQI GTAC 4 cut(s) 390, 396, 1019, 1153
DdeI CTNAG 3 cut(s) 246, 485, 762
DinI GGCGCC 1 cut(s) 74
DpnI GATC 6 cut(s) 63, 278, 600, 732, 801, 1196
DpnII GATC 6 cut(s) 61, 276, 598, 730, 799, 1194
DraI TTTAAA 1 cut(s) 1167
DriI GACNNNNNGTC 1 cut(s) 703
Eam1104I CTCTTC 1 cut(s) 2
Eam1105I GACNNNNNGTC 1 cut(s) 703
EarI CTCTTC 1 cut(s) 2
Eco130I CCWWGG 3 cut(s) 180, 226, 1008
Eco31I GGTCTC 2 cut(s) 944, 992
Eco47I GGWCC 1 cut(s) 1213
Eco57I CTGAAG 2 cut(s) 480, 812
EcoRII CCWGG 2 cut(s) 508, 975
EcoT14I CCWWGG 3 cut(s) 180, 226, 1008
EgeI GGCGCC 1 cut(s) 74
EheI GGCGCC 1 cut(s) 74
ErhI CCWWGG 3 cut(s) 180, 226, 1008
Esp3I CGTCTC 2 cut(s) 109, 251
FaeI CATG 5 cut(s) 184, 341, 598, 1085, 1109
FalI AAGNNNNNCTT 2 cut(s) 917, 949
FaqI GGGAC 2 cut(s) 1010, 1211
FatI CATG 5 cut(s) 180, 337, 594, 1081, 1105
FbaI TGATCA 3 cut(s) 61, 276, 730
Fnu4HI GCNGC 4 cut(s) 291, 361, 465, 786
FokI GGATG 1 cut(s) 694
Fsp4HI GCNGC 4 cut(s) 291, 361, 465, 786
FspBI CTAG 1 cut(s) 138
FspI TGCGCA 1 cut(s) 152
GlaI GCGC 2 cut(s) 74, 152
GluI GCNGC 4 cut(s) 291, 361, 465, 786
GsaI CCCAGC 1 cut(s) 235
GsuI CTGGAG 1 cut(s) 998
HaeII RGCGCY 1 cut(s) 76
HapII CCGG 2 cut(s) 613, 711
HgaI GACGC 1 cut(s) 1213
HhaI GCGC 2 cut(s) 75, 153
Hin1I GRCGYC 1 cut(s) 73
Hin1II CATG 5 cut(s) 184, 341, 598, 1085, 1109
Hin6I GCGC 2 cut(s) 73, 151
HinP1I GCGC 2 cut(s) 73, 151
HindIII AAGCTT 1 cut(s) 1068
HinfI GANTC 2 cut(s) 95, 1013
HpaII CCGG 2 cut(s) 613, 711
HphI GGTGA 1 cut(s) 341
Hpy188I TCNGA 3 cut(s) 792, 881, 1116
Hpy188III TCNNGA 6 cut(s) 89, 338, 711, 995, 1052, 1106
HpyAV CCTTC 3 cut(s) 787, 1001, 1112
HpyCH4III ACNGT 4 cut(s) 329, 409, 457, 1157
HpyCH4IV ACGT 1 cut(s) 260
HpyF10VI GCNNNNNNNGC 3 cut(s) 290, 313, 945
HpyF3I CTNAG 3 cut(s) 246, 485, 762
HpySE526I ACGT 1 cut(s) 260
Hsp92I GRCGYC 1 cut(s) 73
Hsp92II CATG 5 cut(s) 184, 341, 598, 1085, 1109
HspAI GCGC 2 cut(s) 73, 151
KasI GGCGCC 1 cut(s) 72
Kpn2I TCCGGA 1 cut(s) 710
Ksp22I TGATCA 3 cut(s) 61, 276, 730
Kzo9I GATC 6 cut(s) 61, 276, 598, 730, 799, 1194
LmnI GCTCC 4 cut(s) 91, 265, 766, 972
Lsp1109I GCAGC 3 cut(s) 372, 476, 797
LweI GCATC 4 cut(s) 277, 347, 949, 1114
MaeI CTAG 1 cut(s) 138
MaeII ACGT 1 cut(s) 260
MaeIII GTNAC 4 cut(s) 167, 369, 735, 1093
MalI GATC 6 cut(s) 63, 278, 600, 732, 801, 1196
MbiI CCGCTC 1 cut(s) 293
MboI GATC 6 cut(s) 61, 276, 598, 730, 799, 1194
MboII GAAGA 6 cut(s) 19, 227, 356, 701, 809, 1040
MfeI CAATTG 1 cut(s) 201
MflI RGATCY 2 cut(s) 598, 799
MluCI AATT 9 cut(s) 17, 186, 201, 348, 433, 543, 883, 965, 1140
MluI ACGCGT 1 cut(s) 1222
Mly113I GGCGCC 1 cut(s) 73
MmeI TCCRAC 3 cut(s) 154, 243, 1155
MroI TCCGGA 1 cut(s) 710
MseI TTAA 1 cut(s) 1166
MslI CAYNNNNRTG 2 cut(s) 559, 909
MspA1I CMGCKG 1 cut(s) 250
MspI CCGG 2 cut(s) 613, 711
MspR9I CCNGG 2 cut(s) 510, 977
MunI CAATTG 1 cut(s) 201
MvaI CCWGG 2 cut(s) 510, 977
MvnI CGCG 1 cut(s) 1224
MwoI GCNNNNNNNGC 3 cut(s) 290, 313, 945
NarI GGCGCC 1 cut(s) 73
NcoI CCATGG 1 cut(s) 180
NdeII GATC 6 cut(s) 61, 276, 598, 730, 799, 1194
NlaIII CATG 5 cut(s) 184, 341, 598, 1085, 1109
NlaIV GGNNCC 4 cut(s) 74, 239, 507, 974
NmuCI GTSAC 1 cut(s) 369
NsbI TGCGCA 1 cut(s) 152
PagI TCATGA 2 cut(s) 337, 1105
PaqCI CACCTGC 1 cut(s) 559
PfeI GAWTC 2 cut(s) 95, 1013
PkrI GCNGC 4 cut(s) 292, 362, 466, 787
PluTI GGCGCC 1 cut(s) 76
PsiI TTATAA 1 cut(s) 869
Psp6I CCWGG 2 cut(s) 508, 975
PspFI CCCAGC 1 cut(s) 231
PspGI CCWGG 2 cut(s) 508, 975
PspN4I GGNNCC 4 cut(s) 74, 239, 507, 974
PspPI GGNCC 1 cut(s) 1213
PstI CTGCAG 1 cut(s) 286
PstNI CAGNNNCTG 1 cut(s) 1022
PsuI RGATCY 2 cut(s) 598, 799
PvuII CAGCTG 1 cut(s) 250
RsaI GTAC 4 cut(s) 391, 397, 1020, 1154
RsaNI GTAC 4 cut(s) 390, 396, 1019, 1153
RseI CAYNNNNRTG 2 cut(s) 559, 909
SaqAI TTAA 1 cut(s) 1166
SatI GCNGC 4 cut(s) 291, 361, 465, 786
Sau3AI GATC 6 cut(s) 61, 276, 598, 730, 799, 1194
Sau96I GGNCC 1 cut(s) 1213
ScaI AGTACT 1 cut(s) 391
ScrFI CCNGG 2 cut(s) 510, 977
SfaNI GCATC 4 cut(s) 277, 347, 949, 1114
SfcI CTRYAG 2 cut(s) 38, 282
SfoI GGCGCC 1 cut(s) 74
SinI GGWCC 1 cut(s) 1213
SmiMI CAYNNNNRTG 2 cut(s) 559, 909
Sse9I AATT 9 cut(s) 17, 186, 201, 348, 433, 543, 883, 965, 1140
SsiI CCGC 2 cut(s) 114, 291
SspDI GGCGCC 1 cut(s) 72
SspMI CTAG 1 cut(s) 138
StyD4I CCNGG 2 cut(s) 508, 975
StyI CCWWGG 3 cut(s) 180, 226, 1008
TaaI ACNGT 4 cut(s) 329, 409, 457, 1157
TaiI ACGT 1 cut(s) 263
TaqI TCGA 4 cut(s) 384, 501, 591, 627
TasI AATT 9 cut(s) 17, 186, 201, 348, 433, 543, 883, 965, 1140
TatI WGTACW 1 cut(s) 389
TauI GCSGC 1 cut(s) 293
TfiI GAWTC 2 cut(s) 95, 1013
Tru1I TTAA 1 cut(s) 1166
Tru9I TTAA 1 cut(s) 1166
TscAI CASTG 3 cut(s) 83, 286, 493
TseFI GTSAC 1 cut(s) 369
TseI GCWGC 3 cut(s) 360, 464, 785
Tsp45I GTSAC 1 cut(s) 369
TspDTI ATGAA 3 cut(s) 326, 930, 1094
TspRI CASTG 3 cut(s) 83, 286, 493
VpaK11BI GGWCC 1 cut(s) 1213
XapI RAATTY 1 cut(s) 883
XspI CTAG 1 cut(s) 138
ZrmI AGTACT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.