MD03G1200100.v1.1

HAUS augmin-like complex subunit 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002315
Physical Location & Seq
Forward (+)
27384577 .. 27388598
4022 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1200100.v1.1.491

Sequence Viewer

Length: 1230 bp
ATGGCGAAGGGCCTGCAACAACTACAGGGACAAAACCTGCCACCGGATGTGGCCCATGTTGTCGATCAGCTGGAACGCCACTGCTTGGCTCCCGATGGTTCCCTCGGCTCCAAATCCGCATATTACGACCTCCAACTCGCCAGGGAGGCAATGTGCGGGGAAAGAATCCGTTACTTGGAAGCCATGGCAATTTATGGCGAGGCAATTGCAATGGTGGAGGAGTATCAACAGGGATTGAAGCATTCTTCTGAGGCTGAGCTGTACGAGACATTAGAGCATCGTATGATTGTTGCAGAAGCAGCCCAAAGGTCTAGGCTTCCTCTTGTCTCCAAAGATGGTGAAGTTCATGAGGAAGAGATTGAAAAATGCAGTATAATGTCACGGAGTTCCCTTGACAGTACTTGTACCGGTGTTACAATCAGCTCAAGTTCCAACTCCACAAATTATACAGCAAGCAACAGTCTTTCTTGTAGTGCTACTGATATTGTGGAACCTGGACTTGGTGGTGTACCCAATCGCTTTCTTGGAATTACACCTGCTTATTTATGGCAAACACAGCTCCAGCAATCTCCCTTTTGTTTGGATATGAAAGAATACCAGTTGTGTCTTTCCCATGAGATTGAGGCTCGGTTGGAAGAAAAATGTGACAAGTTAGCTGATGCATTAGTAATTGATGACATTGATTCATCACCCCTGCATGAAACTACAAGTTCTCAGCTTCCAGAAAGGGTAAAGTTGATAACTGAGGAGATAGAAAGGGAGGAAGAAGCTTTGCAGGAAGATCTATATTCCGCAGATAGAAAATTTTCTGAATACTATAACGTCTTAGAGCAGATACTGGGAGTTCTCGTCAAGTTGATCAAAGATTTGAAATTGCACCATCAACACAATTATGATGAACTACAAAAAACATGGCTGTGCAAAAGGTGTGTGACCATGAGCGCAAAACTGAGGAAACTGGAGTATGCTATCGTTGGTCAAACGTATACTAAGGAATCTGTACCAGCCCTGCATAAAATTAGGAAGTATCTTACTGATGCTACGGAAGAAGCTTCGGTTACATATAATAAAGCGGTTACGCGCCTTCATGAGTATCAGGGTGTCGATCCTCACTTCGATCAAATTGCAAGGCGGTACCGTGATATTGTAAAGAAACTGGAAAACATGAATTGGACGATCCACCAAGTTGAAATGGACCTGAAACGCCTCCCGGATCATCCGAACGCCTAG

Protein Analysis

410

Amino Acids

46.58

Weight (kDa)

5.24

Isoelectric Point (pI)

52.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS4 PF14735 164 - 398 2.8e-77 HAUS augmin-like complex subunit 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 544
Acc36I ACCTGC 2 cut(s) 45, 544
Acc65I GGTACC 1 cut(s) 1134
AccB1I GGYRCC 1 cut(s) 1134
AccB7I CCANNNNNTGG 1 cut(s) 85
AccI GTMKAC 1 cut(s) 986
AccII CGCG 1 cut(s) 1081
AciI CCGC 5 cut(s) 117, 156, 792, 1073, 1132
AclWI GGATC 3 cut(s) 1100, 1171, 1221
AcsI RAATTY 1 cut(s) 803
AfaI GTAC 6 cut(s) 263, 400, 406, 510, 1002, 1136
AfiI CCNNNNNNNGG 4 cut(s) 43, 85, 175, 500
AgeI ACCGGT 1 cut(s) 407
AgsI TTSAA 4 cut(s) 238, 362, 871, 1190
AjnI CCWGG 2 cut(s) 140, 493
AjuI GAANNNNNNNTTGG 2 cut(s) 483, 515
AluBI AGCT 8 cut(s) 70, 259, 423, 559, 656, 718, 770, 1052
AluI AGCT 8 cut(s) 70, 259, 423, 559, 656, 718, 770, 1052
Alw26I GTCTC 2 cut(s) 260, 331
AlwI GGATC 3 cut(s) 1100, 1171, 1221
AlwNI CAGNNNCTG 1 cut(s) 838
AoxI GGCC 2 cut(s) 10, 51
ApeKI GCWGC 1 cut(s) 299
ApoI RAATTY 1 cut(s) 803
AsiGI ACCGGT 1 cut(s) 407
Asp700I GAANNNNTTC 1 cut(s) 805
Asp718I GGTACC 1 cut(s) 1134
AspLEI GCGC 2 cut(s) 944, 1083
AspS9I GGNCC 3 cut(s) 10, 52, 1195
AsuC2I CCSGG 1 cut(s) 1211
AsuHPI GGTGA 2 cut(s) 350, 681
AvaII GGWCC 1 cut(s) 1195
BanI GGYRCC 1 cut(s) 1134
BbvI GCAGC 1 cut(s) 311
BccI CCATC 3 cut(s) 89, 329, 888
BcgI CGANNNNNNTGC 5 cut(s) 29, 188, 222, 1106, 1140
BciT130I CCWGG 2 cut(s) 142, 495
BclI TGATCA 1 cut(s) 858
BcnI CCSGG 1 cut(s) 1211
BcoDI GTCTC 2 cut(s) 260, 331
BfaI CTAG 2 cut(s) 312, 1228
BfmI CTRYAG 1 cut(s) 23
BfuAI ACCTGC 2 cut(s) 45, 544
BglI GCCNNNNNGGC 1 cut(s) 146
BglII AGATCT 1 cut(s) 781
BisI GCNGC 1 cut(s) 300
BlpI GCTNAGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 301
BmcAI AGTACT 1 cut(s) 400
Bme1390I CCNGG 3 cut(s) 142, 495, 1211
Bme18I GGWCC 1 cut(s) 1195
BmgT120I GGNCC 3 cut(s) 10, 52, 1195
BmiI GGNNCC 5 cut(s) 90, 100, 109, 492, 1136
BmrFI CCNGG 3 cut(s) 142, 495, 1211
BmrI ACTGGG 1 cut(s) 848
BmsI GCATC 3 cut(s) 286, 649, 1027
BmuI ACTGGG 1 cut(s) 848
BpmI CTGGAG 2 cut(s) 545, 980
Bpu1102I GCTNAGC 1 cut(s) 255
BpuEI CTTGAG 1 cut(s) 409
BpuMI CCSGG 1 cut(s) 1211
BsaJI CCNNGG 3 cut(s) 103, 141, 183
BsaWI WCCGGW 2 cut(s) 43, 407
BsaXI ACNNNNNCTCC 4 cut(s) 137, 167, 834, 864
Bsc4I CCNNNNNNNGG 4 cut(s) 43, 85, 175, 500
Bse118I RCCGGY 1 cut(s) 407
Bse1I ACTGG 4 cut(s) 598, 843, 963, 1161
Bse3DI GCAATG 2 cut(s) 156, 216
BseBI CCWGG 2 cut(s) 142, 495
BseDI CCNNGG 3 cut(s) 103, 141, 183
BseGI GGATG 2 cut(s) 52, 1216
BseLI CCNNNNNNNGG 4 cut(s) 43, 85, 175, 500
BseMI GCAATG 2 cut(s) 156, 216
BseMII CTCAG 5 cut(s) 240, 246, 728, 735, 941
BseNI ACTGG 4 cut(s) 598, 843, 963, 1161
BseRI GAGGAG 2 cut(s) 233, 761
BseXI GCAGC 1 cut(s) 311
Bsh1236I CGCG 1 cut(s) 1081
BshFI GGCC 2 cut(s) 12, 53
BshNI GGYRCC 1 cut(s) 1134
BshTI ACCGGT 1 cut(s) 407
BsiSI CCGG 3 cut(s) 44, 408, 1211
BslFI GGGAC 1 cut(s) 42
BslI CCNNNNNNNGG 4 cut(s) 43, 85, 175, 500
BsmAI GTCTC 2 cut(s) 260, 331
BsmFI GGGAC 1 cut(s) 42
BsmI GAATGC 1 cut(s) 241
BsnI GGCC 2 cut(s) 12, 53
Bsp143I GATC 7 cut(s) 64, 781, 858, 1105, 1117, 1176, 1213
Bsp1720I GCTNAGC 1 cut(s) 255
Bsp19I CCATGG 1 cut(s) 183
BspACI CCGC 5 cut(s) 117, 156, 792, 1073, 1132
BspANI GGCC 2 cut(s) 12, 53
BspCNI CTCAG 5 cut(s) 241, 247, 727, 736, 942
BspFNI CGCG 1 cut(s) 1081
BspHI TCATGA 2 cut(s) 346, 1087
BspLI GGNNCC 5 cut(s) 90, 100, 109, 492, 1136
BspMI ACCTGC 2 cut(s) 45, 544
BspPI GGATC 3 cut(s) 1100, 1171, 1221
BspT107I GGYRCC 1 cut(s) 1134
BsrDI GCAATG 2 cut(s) 156, 216
BsrFI RCCGGY 1 cut(s) 407
BsrI ACTGG 4 cut(s) 598, 843, 963, 1161
BssAI RCCGGY 1 cut(s) 407
BssECI CCNNGG 3 cut(s) 103, 141, 183
BssMI GATC 7 cut(s) 64, 781, 858, 1105, 1117, 1176, 1213
BssNAI GTATAC 1 cut(s) 987
BssT1I CCWWGG 1 cut(s) 183
Bst1107I GTATAC 1 cut(s) 987
Bst2UI CCWGG 2 cut(s) 142, 495
Bst4CI ACNGT 3 cut(s) 398, 461, 1139
Bst6I CTCTTC 1 cut(s) 348
BstC8I GCNNGC 2 cut(s) 14, 454
BstDEI CTNAG 7 cut(s) 249, 255, 714, 744, 826, 950, 990
BstDSI CCRYGG 1 cut(s) 183
BstF5I GGATG 2 cut(s) 52, 1216
BstFNI CGCG 1 cut(s) 1081
BstHHI GCGC 2 cut(s) 944, 1083
BstKTI GATC 7 cut(s) 67, 784, 861, 1108, 1120, 1179, 1216
BstMAI GTCTC 2 cut(s) 260, 331
BstMBI GATC 7 cut(s) 64, 781, 858, 1105, 1117, 1176, 1213
BstMWI GCNNNNNNNGC 3 cut(s) 146, 299, 556
BstNI CCWGG 2 cut(s) 142, 495
BstSCI CCNGG 3 cut(s) 140, 493, 1209
BstSFI CTRYAG 1 cut(s) 23
BstUI CGCG 1 cut(s) 1081
BstV1I GCAGC 1 cut(s) 311
BstX2I RGATCY 1 cut(s) 781
BstYI RGATCY 1 cut(s) 781
BstZ17I GTATAC 1 cut(s) 987
BsuRI GGCC 2 cut(s) 12, 53
BtgI CCRYGG 1 cut(s) 183
BtsCI GGATG 2 cut(s) 52, 1216
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 1 cut(s) 79
BveI ACCTGC 2 cut(s) 45, 544
Cac8I GCNNGC 2 cut(s) 14, 454
CaiI CAGNNNCTG 1 cut(s) 838
CciI TCATGA 2 cut(s) 346, 1087
CfoI GCGC 2 cut(s) 944, 1083
Cfr10I RCCGGY 1 cut(s) 407
Cfr13I GGNCC 3 cut(s) 10, 52, 1195
Csp6I GTAC 6 cut(s) 262, 399, 405, 509, 1001, 1135
CspAI ACCGGT 1 cut(s) 407
CviAII CATG 9 cut(s) 56, 184, 347, 614, 698, 912, 937, 1088, 1165
CviQI GTAC 6 cut(s) 262, 399, 405, 509, 1001, 1135
DdeI CTNAG 7 cut(s) 249, 255, 714, 744, 826, 950, 990
DpnI GATC 7 cut(s) 66, 783, 860, 1107, 1119, 1178, 1215
DpnII GATC 7 cut(s) 64, 781, 858, 1105, 1117, 1176, 1213
Eam1104I CTCTTC 1 cut(s) 348
EarI CTCTTC 1 cut(s) 348
Eco130I CCWWGG 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 1195
EcoO109I RGGNCCY 1 cut(s) 10
EcoRII CCWGG 2 cut(s) 140, 493
EcoT14I CCWWGG 1 cut(s) 183
EcoT22I ATGCAT 1 cut(s) 664
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 9 cut(s) 59, 187, 350, 617, 701, 915, 940, 1091, 1168
FaqI GGGAC 1 cut(s) 42
FatI CATG 9 cut(s) 55, 183, 346, 613, 697, 911, 936, 1087, 1164
FauI CCCGC 1 cut(s) 149
FbaI TGATCA 1 cut(s) 858
FblI GTMKAC 1 cut(s) 986
Fnu4HI GCNGC 1 cut(s) 300
FokI GGATG 2 cut(s) 59, 1203
Fsp4HI GCNGC 1 cut(s) 300
FspBI CTAG 2 cut(s) 312, 1228
GlaI GCGC 2 cut(s) 943, 1082
GluI GCNGC 1 cut(s) 300
GsuI CTGGAG 2 cut(s) 545, 980
HaeIII GGCC 2 cut(s) 12, 53
HapII CCGG 3 cut(s) 44, 408, 1211
HhaI GCGC 2 cut(s) 944, 1083
Hin1II CATG 9 cut(s) 59, 187, 350, 617, 701, 915, 940, 1091, 1168
Hin6I GCGC 2 cut(s) 942, 1081
HinP1I GCGC 2 cut(s) 942, 1081
HindIII AAGCTT 2 cut(s) 768, 1050
HinfI GANTC 3 cut(s) 165, 683, 995
HpaII CCGG 3 cut(s) 44, 408, 1211
HphI GGTGA 2 cut(s) 350, 681
Hpy166II GTNNAC 2 cut(s) 509, 987
Hpy188I TCNGA 3 cut(s) 250, 811, 1221
Hpy188III TCNNGA 4 cut(s) 92, 347, 722, 1088
Hpy8I GTNNAC 2 cut(s) 509, 987
HpyAV CCTTC 1 cut(s) 1094
HpyCH4III ACNGT 3 cut(s) 398, 461, 1139
HpyCH4IV ACGT 2 cut(s) 822, 983
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 299, 556
HpyF3I CTNAG 7 cut(s) 249, 255, 714, 744, 826, 950, 990
HpySE526I ACGT 2 cut(s) 822, 983
Hsp92II CATG 9 cut(s) 59, 187, 350, 617, 701, 915, 940, 1091, 1168
HspAI GCGC 2 cut(s) 942, 1081
KpnI GGTACC 1 cut(s) 1138
Ksp22I TGATCA 1 cut(s) 858
Kzo9I GATC 7 cut(s) 64, 781, 858, 1105, 1117, 1176, 1213
LmnI GCTCC 3 cut(s) 94, 113, 564
Lsp1109I GCAGC 1 cut(s) 311
LweI GCATC 3 cut(s) 286, 649, 1027
MaeI CTAG 2 cut(s) 312, 1228
MaeII ACGT 2 cut(s) 822, 983
MaeIII GTNAC 7 cut(s) 170, 378, 412, 644, 931, 1057, 1075
MalI GATC 7 cut(s) 66, 783, 860, 1107, 1119, 1178, 1215
MboI GATC 7 cut(s) 64, 781, 858, 1105, 1117, 1176, 1213
MboII GAAGA 6 cut(s) 237, 365, 647, 776, 791, 1058
MfeI CAATTG 1 cut(s) 204
MflI RGATCY 1 cut(s) 781
MmeI TCCRAC 3 cut(s) 157, 456, 612
Mph1103I ATGCAT 1 cut(s) 664
MroXI GAANNNNTTC 1 cut(s) 805
MslI CAYNNNNRTG 2 cut(s) 891, 916
MspA1I CMGCKG 1 cut(s) 70
MspI CCGG 3 cut(s) 44, 408, 1211
MspR9I CCNGG 3 cut(s) 142, 495, 1211
MunI CAATTG 1 cut(s) 204
Mva1269I GAATGC 1 cut(s) 241
MvaI CCWGG 2 cut(s) 142, 495
MvnI CGCG 1 cut(s) 1081
MwoI GCNNNNNNNGC 3 cut(s) 146, 299, 556
NciI CCSGG 1 cut(s) 1211
NcoI CCATGG 1 cut(s) 183
NdeII GATC 7 cut(s) 64, 781, 858, 1105, 1117, 1176, 1213
NlaIII CATG 9 cut(s) 59, 187, 350, 617, 701, 915, 940, 1091, 1168
NlaIV GGNNCC 5 cut(s) 90, 100, 109, 492, 1136
NmeAIII GCCGAG 1 cut(s) 84
NmuCI GTSAC 3 cut(s) 378, 644, 931
NsiI ATGCAT 1 cut(s) 664
PagI TCATGA 2 cut(s) 346, 1087
PaqCI CACCTGC 1 cut(s) 544
PctI GAATGC 1 cut(s) 241
PdmI GAANNNNTTC 1 cut(s) 805
PfeI GAWTC 3 cut(s) 165, 683, 995
PflMI CCANNNNNTGG 1 cut(s) 85
PfoI TCCNGGA 1 cut(s) 1209
PinAI ACCGGT 1 cut(s) 407
PkrI GCNGC 1 cut(s) 301
Psp6I CCWGG 2 cut(s) 140, 493
PspGI CCWGG 2 cut(s) 140, 493
PspN4I GGNNCC 5 cut(s) 90, 100, 109, 492, 1136
PspPI GGNCC 3 cut(s) 10, 52, 1195
PstNI CAGNNNCTG 1 cut(s) 838
PsuI RGATCY 1 cut(s) 781
PvuII CAGCTG 1 cut(s) 70
RsaI GTAC 6 cut(s) 263, 400, 406, 510, 1002, 1136
RsaNI GTAC 6 cut(s) 262, 399, 405, 509, 1001, 1135
RseI CAYNNNNRTG 2 cut(s) 891, 916
SatI GCNGC 1 cut(s) 300
Sau3AI GATC 7 cut(s) 64, 781, 858, 1105, 1117, 1176, 1213
Sau96I GGNCC 3 cut(s) 10, 52, 1195
ScaI AGTACT 1 cut(s) 400
ScrFI CCNGG 3 cut(s) 142, 495, 1211
SfaNI GCATC 3 cut(s) 286, 649, 1027
SfcI CTRYAG 1 cut(s) 23
SinI GGWCC 1 cut(s) 1195
SmiMI CAYNNNNRTG 2 cut(s) 891, 916
SmlI CTYRAG 1 cut(s) 424
SmoI CTYRAG 1 cut(s) 424
SsiI CCGC 5 cut(s) 117, 156, 792, 1073, 1132
SspMI CTAG 2 cut(s) 312, 1228
StyD4I CCNGG 3 cut(s) 140, 493, 1209
StyI CCWWGG 1 cut(s) 183
TaaI ACNGT 3 cut(s) 398, 461, 1139
TaiI ACGT 2 cut(s) 825, 986
TaqI TCGA 3 cut(s) 63, 1104, 1116
TatI WGTACW 1 cut(s) 398
TfiI GAWTC 3 cut(s) 165, 683, 995
TscAI CASTG 1 cut(s) 86
TseFI GTSAC 3 cut(s) 378, 644, 931
TseI GCWGC 1 cut(s) 299
Tsp45I GTSAC 3 cut(s) 378, 644, 931
TspDTI ATGAA 7 cut(s) 335, 602, 675, 714, 912, 1076, 1181
TspGWI ACGGA 3 cut(s) 158, 397, 1058
TspRI CASTG 1 cut(s) 86
Van91I CCANNNNNTGG 1 cut(s) 85
VpaK11BI GGWCC 1 cut(s) 1195
XapI RAATTY 1 cut(s) 803
XmiI GTMKAC 1 cut(s) 986
XmnI GAANNNNTTC 1 cut(s) 805
XspI CTAG 2 cut(s) 312, 1228
ZrmI AGTACT 1 cut(s) 400
Zsp2I ATGCAT 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.