Rh5CG305400

HAUS augmin-like complex subunit 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0002315
Physical Location & Seq
Reverse (-)
33650042 .. 33653744
3703 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG305400.1

Sequence Viewer

Length: 1308 bp
ATGTTGAAGAGACTGCAATTACAAGAACAATGCCAGCTACAGGATTTGAACCAAGTCATTGATCATCTGGGGCGCCACTGCTTTGCTCCCAACGATTCTCTCGTCTCCAAATCCGCATACTACGACCTCCAACTCGCTAGAGAGGAAATGTGCAGGGAACGACTGCGTTACTTGGAGGCCATGGCAATTTATAGTGAGGCAATTGCAATGGTGGAAGATTATCACCAAGGCTGGGGGAACCCTCCTCAGCTGTTTAAGACGTTGGAGCATCTTATGATCACTGCGGAAGCCTCTCAAAGGTTGAGGCTTCCTCTGCTCTCTAAAGACGGTGATATTCATGAGGAAGAAATTGAAAGATGCAGCATAGTGTCACAAAGTTCCCTCGACAGTACTTGTACCAGTTTCACAGTCGGGTCAAGTTCTAACTCTCCAAATTATAGTGGTGCAAATAGCACAGTTAGTGCAGCAAACAACAATCTTTGTCTCAGTGCTACTTCAGTCGAGGAACCTGGACTTGGAGGCGTTCCCAATCGTTTTCTTGGAATTACACCTGCATATTTATGGAAAACACAGCTTCAACAAATCCCTTTCAACATGGATCTGGCAGAATACCGGCTGTGTCTTTCCGATGAGATTGAGGCTCGTTTGCAAGCTAAATGTGATAAGTTAGCCGACGCCTTTATAATGGAAGACATAGACTGGTCGTCATCCGGACAGGAAAATACTAGTGATCAGTTACCAGAAAGGGTAAAGTCAATAACTGAGGAGCTGGAAAGGGAGGAAGCAGCTCTGAAGGAAGATCTGTATTCAGCAGAAAGAAGATTTACTGAATACTACAATGTTTTAGAGCAGATATTGGGAGTTCTTATAAAGCTGGTCAGAAATTTGAAGTTGCAACATCAACACAAATATGATGAACTGCAAAAGATATTCTTGTGCAAAAGGTGCGAGACCATAGATGCAAAATTGAGGAGCCTGGAGTATGGTCTCCTTCGGGACACTTATTCTAAGGATTCAGTACCTGCCCTGCATAAAATAAGGAAATATCTTCTTGAAGCTACAAAAGAAGCTTCAATCACACATGATAAAGCGGTTACTCGCCTTCATGAGTATCAGAGTGTTGATGCTCACTTCAATAAAATTGCAGGGCAGTACCGTGATATTGTAAAGGTAATAGGAATGCTTACAGTTGTCCATTGGTTTCCTAATGCTAAACTAATCAACATTCCTCCACAGAAGTTGGAAAATATGCAATGGACGATCCAGCAAGTAGAAATGGACCTGAAACGCGTTCCAGAACTACCCTAA

Protein Analysis

435

Amino Acids

49.92

Weight (kDa)

5.43

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS4 PF14735 169 - 392 2.7e-67 HAUS augmin-like complex subunit 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 683, 869
AarI CACCTGC 1 cut(s) 559
Acc36I ACCTGC 2 cut(s) 559, 1030
AccB1I GGYRCC 1 cut(s) 72
AccII CGCG 1 cut(s) 1290
AccIII TCCGGA 1 cut(s) 710
AciI CCGC 3 cut(s) 114, 284, 1091
AclWI GGATC 2 cut(s) 606, 1255
AcsI RAATTY 1 cut(s) 883
AcuI CTGAAG 2 cut(s) 480, 812
AcyI GRCGYC 2 cut(s) 73, 675
AfaI GTAC 4 cut(s) 391, 397, 1020, 1154
AfiI CCNNNNNNNGG 4 cut(s) 40, 232, 297, 515
AflIII ACRYGT 1 cut(s) 1288
AgsI TTSAA 9 cut(s) 7, 49, 353, 578, 592, 889, 1055, 1074, 1135
AhdI GACNNNNNGTC 1 cut(s) 703
AhlI ACTAGT 1 cut(s) 725
AjnI CCWGG 2 cut(s) 508, 975
AjuI GAANNNNNNNTTGG 2 cut(s) 498, 530
AluBI AGCT 9 cut(s) 37, 250, 574, 653, 769, 788, 874, 1058, 1070
AluI AGCT 9 cut(s) 37, 250, 574, 653, 769, 788, 874, 1058, 1070
Alw26I GTCTC 5 cut(s) 4, 109, 488, 944, 992
AlwI GGATC 2 cut(s) 606, 1255
AlwNI CAGNNNCTG 1 cut(s) 1022
Aor13HI TCCGGA 1 cut(s) 710
AoxI GGCC 1 cut(s) 177
ApeKI GCWGC 3 cut(s) 360, 464, 785
ApoI RAATTY 1 cut(s) 883
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 1 cut(s) 1279
AsuHPI GGTGA 2 cut(s) 215, 341
AvaII GGWCC 1 cut(s) 1279
BanI GGYRCC 1 cut(s) 72
BbsI GAAGAC 1 cut(s) 696
BbvCI CCTCAGC 1 cut(s) 246
BbvI GCAGC 3 cut(s) 372, 476, 797
BciT130I CCWGG 2 cut(s) 510, 977
BclI TGATCA 3 cut(s) 61, 276, 730
BcoDI GTCTC 5 cut(s) 4, 109, 488, 944, 992
BcuI ACTAGT 1 cut(s) 725
BfaI CTAG 2 cut(s) 138, 726
BfmI CTRYAG 1 cut(s) 38
BfoI RGCGCY 1 cut(s) 76
BfuAI ACCTGC 2 cut(s) 559, 1030
BglII AGATCT 1 cut(s) 799
BisI GCNGC 3 cut(s) 361, 465, 786
BlsI GCNGC 3 cut(s) 362, 466, 787
BmcAI AGTACT 1 cut(s) 391
Bme1390I CCNGG 2 cut(s) 510, 977
Bme18I GGWCC 1 cut(s) 1279
BmeRI GACNNNNNGTC 1 cut(s) 703
BmgT120I GGNCC 1 cut(s) 1279
BmiI GGNNCC 4 cut(s) 74, 239, 507, 974
BmrFI CCNGG 2 cut(s) 510, 977
BmsI GCATC 4 cut(s) 277, 347, 949, 1114
BpiI GAAGAC 1 cut(s) 696
BplI GAGNNNNNCTC 2 cut(s) 295, 327
BpmI CTGGAG 1 cut(s) 998
Bpu10I CCTNAGC 1 cut(s) 246
BsaHI GRCGYC 2 cut(s) 73, 675
BsaI GGTCTC 2 cut(s) 944, 992
BsaJI CCNNGG 2 cut(s) 180, 226
BsaWI WCCGGW 1 cut(s) 710
Bsc4I CCNNNNNNNGG 4 cut(s) 40, 232, 297, 515
Bse118I RCCGGY 1 cut(s) 612
Bse1I ACTGG 2 cut(s) 399, 704
Bse3DI GCAATG 2 cut(s) 213, 1259
BseAI TCCGGA 1 cut(s) 710
BseBI CCWGG 2 cut(s) 510, 977
BseDI CCNNGG 2 cut(s) 180, 226
BseGI GGATG 1 cut(s) 707
BseLI CCNNNNNNNGG 4 cut(s) 40, 232, 297, 515
BseMI GCAATG 2 cut(s) 213, 1259
BseMII CTCAG 3 cut(s) 260, 499, 753
BseNI ACTGG 2 cut(s) 399, 704
BseRI GAGGAG 3 cut(s) 234, 779, 985
BseXI GCAGC 3 cut(s) 372, 476, 797
BseYI CCCAGC 1 cut(s) 231
BsgI GTGCAG 2 cut(s) 172, 483
Bsh1236I CGCG 1 cut(s) 1290
BshFI GGCC 1 cut(s) 179
BshNI GGYRCC 1 cut(s) 72
BsiSI CCGG 2 cut(s) 613, 711
BslFI GGGAC 1 cut(s) 1010
BslI CCNNNNNNNGG 4 cut(s) 40, 232, 297, 515
BsmAI GTCTC 5 cut(s) 4, 109, 488, 944, 992
BsmBI CGTCTC 1 cut(s) 109
BsmFI GGGAC 1 cut(s) 1010
BsmI GAATGC 1 cut(s) 1185
BsnI GGCC 1 cut(s) 179
Bso31I GGTCTC 2 cut(s) 944, 992
Bsp13I TCCGGA 1 cut(s) 710
Bsp143I GATC 6 cut(s) 61, 276, 598, 730, 799, 1260
Bsp19I CCATGG 1 cut(s) 180
BspACI CCGC 3 cut(s) 114, 284, 1091
BspANI GGCC 1 cut(s) 179
BspCNI CTCAG 3 cut(s) 259, 498, 754
BspEI TCCGGA 1 cut(s) 710
BspFNI CGCG 1 cut(s) 1290
BspHI TCATGA 2 cut(s) 337, 1105
BspLI GGNNCC 4 cut(s) 74, 239, 507, 974
BspMI ACCTGC 2 cut(s) 559, 1030
BspPI GGATC 2 cut(s) 606, 1255
BspT107I GGYRCC 1 cut(s) 72
BspTNI GGTCTC 2 cut(s) 944, 992
BsrDI GCAATG 2 cut(s) 213, 1259
BsrFI RCCGGY 1 cut(s) 612
BsrI ACTGG 2 cut(s) 399, 704
BssAI RCCGGY 1 cut(s) 612
BssECI CCNNGG 2 cut(s) 180, 226
BssMI GATC 6 cut(s) 61, 276, 598, 730, 799, 1260
BssNI GRCGYC 2 cut(s) 73, 675
BssT1I CCWWGG 2 cut(s) 180, 226
Bst2UI CCWGG 2 cut(s) 510, 977
Bst4CI ACNGT 6 cut(s) 329, 389, 409, 457, 1157, 1189
Bst6I CTCTTC 1 cut(s) 2
BstACI GRCGYC 2 cut(s) 73, 675
BstAPI GCANNNNNTGC 1 cut(s) 945
BstC8I GCNNGC 2 cut(s) 35, 651
BstDEI CTNAG 4 cut(s) 246, 485, 762, 1008
BstDSI CCRYGG 1 cut(s) 180
BstENI CCTNNNNNAGG 1 cut(s) 295
BstF5I GGATG 1 cut(s) 707
BstFNI CGCG 1 cut(s) 1290
BstH2I RGCGCY 1 cut(s) 76
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 6 cut(s) 64, 279, 601, 733, 802, 1263
BstMAI GTCTC 5 cut(s) 4, 109, 488, 944, 992
BstMBI GATC 6 cut(s) 61, 276, 598, 730, 799, 1260
BstMWI GCNNNNNNNGC 2 cut(s) 313, 945
BstNI CCWGG 2 cut(s) 510, 977
BstSCI CCNGG 2 cut(s) 508, 975
BstSFI CTRYAG 1 cut(s) 38
BstUI CGCG 1 cut(s) 1290
BstV1I GCAGC 3 cut(s) 372, 476, 797
BstV2I GAAGAC 1 cut(s) 696
BstX2I RGATCY 2 cut(s) 598, 799
BstYI RGATCY 2 cut(s) 598, 799
BsuRI GGCC 1 cut(s) 179
BtgI CCRYGG 1 cut(s) 180
BtsCI GGATG 1 cut(s) 707
BtsI GCAGTG 2 cut(s) 76, 279
BtsIMutI CAGTG 3 cut(s) 76, 279, 493
BveI ACCTGC 2 cut(s) 559, 1030
Cac8I GCNNGC 2 cut(s) 35, 651
CaiI CAGNNNCTG 1 cut(s) 1022
CciI TCATGA 2 cut(s) 337, 1105
CfoI GCGC 1 cut(s) 75
Cfr10I RCCGGY 1 cut(s) 612
Cfr13I GGNCC 1 cut(s) 1279
CseI GACGC 1 cut(s) 683
Csp6I GTAC 4 cut(s) 390, 396, 1019, 1153
CspCI CAANNNNNGTGG 2 cut(s) 1221, 1256
CviAII CATG 5 cut(s) 181, 338, 595, 1082, 1106
CviQI GTAC 4 cut(s) 390, 396, 1019, 1153
DdeI CTNAG 4 cut(s) 246, 485, 762, 1008
DinI GGCGCC 1 cut(s) 74
DpnI GATC 6 cut(s) 63, 278, 600, 732, 801, 1262
DpnII GATC 6 cut(s) 61, 276, 598, 730, 799, 1260
DriI GACNNNNNGTC 1 cut(s) 703
Eam1104I CTCTTC 1 cut(s) 2
Eam1105I GACNNNNNGTC 1 cut(s) 703
EarI CTCTTC 1 cut(s) 2
Eco130I CCWWGG 2 cut(s) 180, 226
Eco31I GGTCTC 2 cut(s) 944, 992
Eco47I GGWCC 1 cut(s) 1279
Eco57I CTGAAG 2 cut(s) 480, 812
EcoNI CCTNNNNNAGG 1 cut(s) 295
EcoRII CCWGG 2 cut(s) 508, 975
EcoT14I CCWWGG 2 cut(s) 180, 226
EgeI GGCGCC 1 cut(s) 74
EheI GGCGCC 1 cut(s) 74
ErhI CCWWGG 2 cut(s) 180, 226
Esp3I CGTCTC 1 cut(s) 109
FaeI CATG 5 cut(s) 184, 341, 598, 1085, 1109
FalI AAGNNNNNCTT 2 cut(s) 917, 949
FaqI GGGAC 1 cut(s) 1010
FatI CATG 5 cut(s) 180, 337, 594, 1081, 1105
FbaI TGATCA 3 cut(s) 61, 276, 730
Fnu4HI GCNGC 3 cut(s) 361, 465, 786
FokI GGATG 1 cut(s) 694
Fsp4HI GCNGC 3 cut(s) 361, 465, 786
FspBI CTAG 2 cut(s) 138, 726
GlaI GCGC 1 cut(s) 74
GluI GCNGC 3 cut(s) 361, 465, 786
GsaI CCCAGC 1 cut(s) 235
GsuI CTGGAG 1 cut(s) 998
HaeII RGCGCY 1 cut(s) 76
HaeIII GGCC 1 cut(s) 179
HapII CCGG 2 cut(s) 613, 711
HgaI GACGC 1 cut(s) 683
HhaI GCGC 1 cut(s) 75
Hin1I GRCGYC 2 cut(s) 73, 675
Hin1II CATG 5 cut(s) 184, 341, 598, 1085, 1109
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HindIII AAGCTT 1 cut(s) 1068
HinfI GANTC 2 cut(s) 95, 1013
HpaII CCGG 2 cut(s) 613, 711
HphI GGTGA 2 cut(s) 215, 341
Hpy188I TCNGA 4 cut(s) 628, 792, 881, 1116
Hpy188III TCNNGA 6 cut(s) 338, 711, 995, 1052, 1106, 1295
Hpy99I CGWCG 1 cut(s) 677
HpyAV CCTTC 3 cut(s) 787, 1001, 1112
HpyCH4III ACNGT 6 cut(s) 329, 389, 409, 457, 1157, 1189
HpyCH4IV ACGT 1 cut(s) 260
HpyF10VI GCNNNNNNNGC 2 cut(s) 313, 945
HpyF3I CTNAG 4 cut(s) 246, 485, 762, 1008
HpySE526I ACGT 1 cut(s) 260
Hsp92I GRCGYC 2 cut(s) 73, 675
Hsp92II CATG 5 cut(s) 184, 341, 598, 1085, 1109
HspAI GCGC 1 cut(s) 73
KasI GGCGCC 1 cut(s) 72
Kpn2I TCCGGA 1 cut(s) 710
Ksp22I TGATCA 3 cut(s) 61, 276, 730
Kzo9I GATC 6 cut(s) 61, 276, 598, 730, 799, 1260
LmnI GCTCC 4 cut(s) 91, 265, 766, 972
Lsp1109I GCAGC 3 cut(s) 372, 476, 797
LweI GCATC 4 cut(s) 277, 347, 949, 1114
MaeI CTAG 2 cut(s) 138, 726
MaeII ACGT 1 cut(s) 260
MaeIII GTNAC 4 cut(s) 167, 369, 735, 1093
MalI GATC 6 cut(s) 63, 278, 600, 732, 801, 1262
MboI GATC 6 cut(s) 61, 276, 598, 730, 799, 1260
MboII GAAGA 7 cut(s) 19, 227, 356, 701, 809, 831, 1040
MfeI CAATTG 1 cut(s) 201
MflI RGATCY 2 cut(s) 598, 799
MluCI AATT 9 cut(s) 17, 186, 201, 348, 433, 543, 883, 965, 1140
MluI ACGCGT 1 cut(s) 1288
Mly113I GGCGCC 1 cut(s) 73
MmeI TCCRAC 3 cut(s) 154, 243, 1221
MroI TCCGGA 1 cut(s) 710
MseI TTAA 1 cut(s) 255
MslI CAYNNNNRTG 2 cut(s) 559, 909
MspA1I CMGCKG 1 cut(s) 250
MspI CCGG 2 cut(s) 613, 711
MspR9I CCNGG 2 cut(s) 510, 977
MunI CAATTG 1 cut(s) 201
Mva1269I GAATGC 1 cut(s) 1185
MvaI CCWGG 2 cut(s) 510, 977
MvnI CGCG 1 cut(s) 1290
MwoI GCNNNNNNNGC 2 cut(s) 313, 945
NarI GGCGCC 1 cut(s) 73
NcoI CCATGG 1 cut(s) 180
NdeII GATC 6 cut(s) 61, 276, 598, 730, 799, 1260
NlaIII CATG 5 cut(s) 184, 341, 598, 1085, 1109
NlaIV GGNNCC 4 cut(s) 74, 239, 507, 974
NmuCI GTSAC 1 cut(s) 369
PagI TCATGA 2 cut(s) 337, 1105
PaqCI CACCTGC 1 cut(s) 559
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PctI GAATGC 1 cut(s) 1185
PfeI GAWTC 2 cut(s) 95, 1013
PkrI GCNGC 3 cut(s) 362, 466, 787
PluTI GGCGCC 1 cut(s) 76
PsiI TTATAA 2 cut(s) 683, 869
Psp6I CCWGG 2 cut(s) 508, 975
PspFI CCCAGC 1 cut(s) 231
PspGI CCWGG 2 cut(s) 508, 975
PspN4I GGNNCC 4 cut(s) 74, 239, 507, 974
PspPI GGNCC 1 cut(s) 1279
PstNI CAGNNNCTG 1 cut(s) 1022
PsuI RGATCY 2 cut(s) 598, 799
PvuII CAGCTG 1 cut(s) 250
RsaI GTAC 4 cut(s) 391, 397, 1020, 1154
RsaNI GTAC 4 cut(s) 390, 396, 1019, 1153
RseI CAYNNNNRTG 2 cut(s) 559, 909
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 3 cut(s) 361, 465, 786
Sau3AI GATC 6 cut(s) 61, 276, 598, 730, 799, 1260
Sau96I GGNCC 1 cut(s) 1279
ScaI AGTACT 1 cut(s) 391
ScrFI CCNGG 2 cut(s) 510, 977
SfaNI GCATC 4 cut(s) 277, 347, 949, 1114
SfcI CTRYAG 1 cut(s) 38
SfoI GGCGCC 1 cut(s) 74
SinI GGWCC 1 cut(s) 1279
SmiMI CAYNNNNRTG 2 cut(s) 559, 909
SpeI ACTAGT 1 cut(s) 725
Sse9I AATT 9 cut(s) 17, 186, 201, 348, 433, 543, 883, 965, 1140
SsiI CCGC 3 cut(s) 114, 284, 1091
SspDI GGCGCC 1 cut(s) 72
SspMI CTAG 2 cut(s) 138, 726
StyD4I CCNGG 2 cut(s) 508, 975
StyI CCWWGG 2 cut(s) 180, 226
TaaI ACNGT 6 cut(s) 329, 389, 409, 457, 1157, 1189
TaiI ACGT 1 cut(s) 263
TaqI TCGA 2 cut(s) 384, 501
TasI AATT 9 cut(s) 17, 186, 201, 348, 433, 543, 883, 965, 1140
TatI WGTACW 1 cut(s) 389
TfiI GAWTC 2 cut(s) 95, 1013
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 3 cut(s) 83, 286, 493
TseFI GTSAC 1 cut(s) 369
TseI GCWGC 3 cut(s) 360, 464, 785
Tsp45I GTSAC 1 cut(s) 369
TspDTI ATGAA 3 cut(s) 326, 930, 1094
TspRI CASTG 3 cut(s) 83, 286, 493
VpaK11BI GGWCC 1 cut(s) 1279
XagI CCTNNNNNAGG 1 cut(s) 295
XapI RAATTY 1 cut(s) 883
XspI CTAG 2 cut(s) 138, 726
ZrmI AGTACT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.