AT1G54620

invertase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002424
Physical Location & Seq
Reverse (-)
20400211 .. 20401025
815 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G54620.1

Sequence Viewer

Length: 579 bp
ATGTTTTGCTTCCAAAGAAAAAAAGAAACAATGAAGTTCTTGATTTACCTCGTTGTGTTTGTTCTTCTTTTCAATGGTTTGACGGCCAACAGAGTGGAAAACTCTCTGGTTCAGAAATATTGCAATCAAAGTTCAAAGTACGCGGAACGATATTTCTATAAGTTTTGCATTGCATCTCTCGAAGAGAATGCGGAAAGTCAGAAAGCAAGGAATAGCAATGATTTAACATTGGTAGGAACGAATAACGCTTTATCAAACCTAACAAACGTGAAAAGAATTGTGGAGAAGATTTTCAACGAGAGAAAATATAAGGGCAGGCTGAGTAAGAAGTTGTTGCAAAAGTGCCTTAAGCTTTATTCTGAAGGCTATGACGCGTTAACCTCAGCTTTGAAGTACATTAAAGTGAGAGATCTGTTTAAAGTTTCGGTTCATTTGCAGAAAGCAAAAGAAGCACCAAGCATTTGCGAAATGGGATTCAACGGCGACAATAAACAAATATCTCCGATGAAGAAAGAAAACGATGTTCTTTTCGACGTGATCAATATTCCTTACAGTTTTAATATGAATCGACATGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

192

Amino Acids

22.32

Weight (kDa)

9.58

Isoelectric Point (pI)

27.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 35 - 182 1.8e-19 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 143, 374
AciI CCGC 2 cut(s) 143, 191
AcoI YGGCCR 1 cut(s) 84
AcuI CTGAAG 1 cut(s) 381
AfaI GTAC 2 cut(s) 140, 395
AflII CTTAAG 1 cut(s) 347
AflIII ACRYGT 1 cut(s) 372
AgsI TTSAA 5 cut(s) 73, 135, 295, 391, 478
AjiI CACGTC 1 cut(s) 535
AluBI AGCT 2 cut(s) 352, 386
AluI AGCT 2 cut(s) 352, 386
AoxI GGCC 1 cut(s) 84
Asp700I GAANNNNTTC 1 cut(s) 290
BbvCI CCTCAGC 1 cut(s) 382
BceAI ACGGC 2 cut(s) 99, 496
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BclI TGATCA 1 cut(s) 537
BfrI CTTAAG 1 cut(s) 347
BglII AGATCT 1 cut(s) 409
BmgBI CACGTC 1 cut(s) 535
BmsI GCATC 1 cut(s) 182
Bpu10I CCTNAGC 1 cut(s) 382
Bse3DI GCAATG 2 cut(s) 168, 223
BseMI GCAATG 2 cut(s) 168, 223
BseMII CTCAG 2 cut(s) 311, 396
Bsh1236I CGCG 2 cut(s) 143, 374
BshFI GGCC 1 cut(s) 86
BsmI GAATGC 1 cut(s) 193
BsnI GGCC 1 cut(s) 86
Bsp143I GATC 2 cut(s) 409, 537
BspACI CCGC 2 cut(s) 143, 191
BspANI GGCC 1 cut(s) 86
BspCNI CTCAG 2 cut(s) 312, 395
BspFNI CGCG 2 cut(s) 143, 374
BspTI CTTAAG 1 cut(s) 347
BsrDI GCAATG 2 cut(s) 168, 223
BssMI GATC 2 cut(s) 409, 537
Bst4CI ACNGT 1 cut(s) 554
Bst6I CTCTTC 1 cut(s) 177
BstAFI CTTAAG 1 cut(s) 347
BstC8I GCNNGC 1 cut(s) 317
BstDEI CTNAG 2 cut(s) 320, 382
BstFNI CGCG 2 cut(s) 143, 374
BstKTI GATC 2 cut(s) 412, 540
BstMBI GATC 2 cut(s) 409, 537
BstMWI GCNNNNNNNGC 1 cut(s) 449
BstUI CGCG 2 cut(s) 143, 374
BstX2I RGATCY 1 cut(s) 409
BstXI CCANNNNNNTGG 1 cut(s) 94
BstYI RGATCY 1 cut(s) 409
BsuRI GGCC 1 cut(s) 86
BtrI CACGTC 1 cut(s) 535
Cac8I GCNNGC 1 cut(s) 317
CseI GACGC 1 cut(s) 380
Csp6I GTAC 2 cut(s) 139, 394
CviAII CATG 1 cut(s) 572
CviJI RGCY 5 cut(s) 86, 319, 352, 366, 386
CviKI_1 RGCY 5 cut(s) 86, 319, 352, 366, 386
CviQI GTAC 2 cut(s) 139, 394
DdeI CTNAG 2 cut(s) 320, 382
DpnI GATC 2 cut(s) 411, 539
DpnII GATC 2 cut(s) 409, 537
DraI TTTAAA 1 cut(s) 418
EaeI YGGCCR 1 cut(s) 84
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Eco57I CTGAAG 1 cut(s) 381
FaeI CATG 1 cut(s) 575
FaiI YATR 5 cut(s) 159, 309, 369, 563, 573
FatI CATG 1 cut(s) 571
FbaI TGATCA 1 cut(s) 537
HaeIII GGCC 1 cut(s) 86
HgaI GACGC 1 cut(s) 380
Hin1II CATG 1 cut(s) 575
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HindIII AAGCTT 1 cut(s) 350
HinfI GANTC 2 cut(s) 474, 565
HpaI GTTAAC 1 cut(s) 378
Hpy166II GTNNAC 1 cut(s) 378
Hpy188I TCNGA 4 cut(s) 114, 201, 361, 504
Hpy188III TCNNGA 2 cut(s) 40, 179
Hpy8I GTNNAC 1 cut(s) 378
Hpy99I CGWCG 1 cut(s) 536
HpyAV CCTTC 1 cut(s) 356
HpyCH4III ACNGT 1 cut(s) 554
HpyCH4IV ACGT 2 cut(s) 267, 534
HpyCH4V TGCA 5 cut(s) 123, 168, 173, 337, 436
HpyF10VI GCNNNNNNNGC 1 cut(s) 449
HpyF3I CTNAG 2 cut(s) 320, 382
HpySE526I ACGT 2 cut(s) 267, 534
Hsp92II CATG 1 cut(s) 575
Ksp22I TGATCA 1 cut(s) 537
KspAI GTTAAC 1 cut(s) 378
Kzo9I GATC 2 cut(s) 409, 537
LpnPI CCDG 2 cut(s) 92, 301
LweI GCATC 1 cut(s) 182
MaeII ACGT 2 cut(s) 267, 534
MalI GATC 2 cut(s) 411, 539
MboI GATC 2 cut(s) 409, 537
MboII GAAGA 4 cut(s) 56, 194, 298, 520
MflI RGATCY 1 cut(s) 409
MluCI AATT 1 cut(s) 276
MluI ACGCGT 1 cut(s) 372
MnlI CCTC 2 cut(s) 59, 391
MroXI GAANNNNTTC 1 cut(s) 290
MseI TTAA 6 cut(s) 224, 348, 377, 399, 417, 558
MslI CAYNNNNRTG 1 cut(s) 401
MspCI CTTAAG 1 cut(s) 347
Mva1269I GAATGC 1 cut(s) 193
MvnI CGCG 2 cut(s) 143, 374
MwoI GCNNNNNNNGC 1 cut(s) 449
NdeII GATC 2 cut(s) 409, 537
NlaIII CATG 1 cut(s) 575
PctI GAATGC 1 cut(s) 193
PdmI GAANNNNTTC 1 cut(s) 290
PfeI GAWTC 2 cut(s) 474, 565
PsuI RGATCY 1 cut(s) 409
RsaI GTAC 2 cut(s) 140, 395
RsaNI GTAC 2 cut(s) 139, 394
RseI CAYNNNNRTG 1 cut(s) 401
SaqAI TTAA 6 cut(s) 224, 348, 377, 399, 417, 558
Sau3AI GATC 2 cut(s) 409, 537
SetI ASST 7 cut(s) 51, 261, 270, 354, 383, 388, 537
SfaNI GCATC 1 cut(s) 182
SmiMI CAYNNNNRTG 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
Sse9I AATT 1 cut(s) 276
SsiI CCGC 2 cut(s) 143, 191
SspI AATATT 2 cut(s) 119, 544
TaaI ACNGT 1 cut(s) 554
TaiI ACGT 2 cut(s) 270, 537
TaqI TCGA 3 cut(s) 180, 531, 568
TasI AATT 1 cut(s) 276
TatI WGTACW 1 cut(s) 393
TfiI GAWTC 2 cut(s) 474, 565
Tru1I TTAA 6 cut(s) 224, 348, 377, 399, 417, 558
Tru9I TTAA 6 cut(s) 224, 348, 377, 399, 417, 558
TspDTI ATGAA 4 cut(s) 47, 419, 521, 578
Vha464I CTTAAG 1 cut(s) 347
XmnI GAANNNNTTC 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.