Rh7CG249600

invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0002424
Physical Location & Seq
Forward (+)
23534869 .. 23535336
468 bp
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UTR
Exon/CDS
Intron
Rh7CG249600.1

Sequence Viewer

Length: 468 bp
ATGGGCGATGATCTCGTCCAACAAACTTGCAAGAAAGCCTCAGATGCTGATAAAAACTTGAACTACAACTTTTGTGTCTCAGCCCTTGAGGCCAGCCCGAAAAGCAAGGGTGCAGACCTCGCAGGACTAGCTGGCATTGCACTTGACCTTACCACAGCGAAGGCGACAAGCATCAAGTCTACCATTTCAAAGCTCTTGAACGATCCAAAAGTTGACAAGCCTGCGCTACAAGACTGCGAAGAACTCTACTCAGGTGCCACTGATGACCTGCAAGCAGCCACCAATGCTTTCAAGTCCAGTGATTATGGTACAGCCCTTACACATCTGAGTGCTACCTTGAATGCACCTGATACTTGTGAAACCGGGTTCCAAGAGAGGAAAGCAGCGTCTCCTTTGAGCAAGGACGACGGTGACTTCGCTCAGTTGAACATCATATCTCTTGCCTTTATCAACCAATTGCGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

155

Amino Acids

16.32

Weight (kDa)

4.67

Isoelectric Point (pI)

17.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 4 - 148 3.7e-28 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 276
AccB1I GGYRCC 1 cut(s) 254
AccI GTMKAC 1 cut(s) 179
AclWI GGATC 1 cut(s) 197
AfaI GTAC 1 cut(s) 310
AgsI TTSAA 6 cut(s) 61, 189, 199, 292, 340, 427
AluBI AGCT 2 cut(s) 131, 193
AluI AGCT 2 cut(s) 131, 193
Alw26I GTCTC 2 cut(s) 82, 393
AlwI GGATC 1 cut(s) 197
AlwNI CAGNNNCTG 1 cut(s) 47
AoxI GGCC 1 cut(s) 90
ApeKI GCWGC 2 cut(s) 275, 383
ArsI GACNNNNNNTTYG 2 cut(s) 398, 430
AspLEI GCGC 1 cut(s) 226
AsuC2I CCSGG 1 cut(s) 364
AsuHPI GGTGA 1 cut(s) 422
BanI GGYRCC 1 cut(s) 254
BbvI GCAGC 2 cut(s) 287, 395
BcnI CCSGG 1 cut(s) 364
BcoDI GTCTC 2 cut(s) 82, 393
BfaI CTAG 1 cut(s) 128
BfuAI ACCTGC 1 cut(s) 276
BglI GCCNNNNNGGC 1 cut(s) 89
BisI GCNGC 2 cut(s) 276, 384
BlsI GCNGC 2 cut(s) 277, 385
Bme1390I CCNGG 1 cut(s) 364
BmiI GGNNCC 2 cut(s) 256, 368
BmrFI CCNGG 1 cut(s) 364
BmsI GCATC 2 cut(s) 34, 180
BpuEI CTTGAG 1 cut(s) 107
BpuMI CCSGG 1 cut(s) 364
Bse1I ACTGG 1 cut(s) 297
Bse3DI GCAATG 1 cut(s) 135
BseMI GCAATG 1 cut(s) 135
BseMII CTCAG 5 cut(s) 54, 93, 264, 317, 434
BseNI ACTGG 1 cut(s) 297
BseXI GCAGC 2 cut(s) 287, 395
BsgI GTGCAG 1 cut(s) 132
BshFI GGCC 1 cut(s) 92
BshNI GGYRCC 1 cut(s) 254
BsiSI CCGG 1 cut(s) 363
BsmAI GTCTC 2 cut(s) 82, 393
BsmBI CGTCTC 1 cut(s) 393
BsmI GAATGC 1 cut(s) 346
BsnI GGCC 1 cut(s) 92
Bsp143I GATC 2 cut(s) 10, 202
BspANI GGCC 1 cut(s) 92
BspCNI CTCAG 5 cut(s) 53, 92, 263, 318, 433
BspLI GGNNCC 2 cut(s) 256, 368
BspMI ACCTGC 1 cut(s) 276
BspPI GGATC 1 cut(s) 197
BspT107I GGYRCC 1 cut(s) 254
BsrDI GCAATG 1 cut(s) 135
BsrI ACTGG 1 cut(s) 297
BssMI GATC 2 cut(s) 10, 202
Bst4CI ACNGT 1 cut(s) 410
BstC8I GCNNGC 4 cut(s) 94, 133, 222, 273
BstDEI CTNAG 5 cut(s) 40, 79, 250, 326, 420
BstHHI GCGC 1 cut(s) 226
BstKTI GATC 2 cut(s) 13, 205
BstMAI GTCTC 2 cut(s) 82, 393
BstMBI GATC 2 cut(s) 10, 202
BstMWI GCNNNNNNNGC 7 cut(s) 44, 89, 102, 119, 128, 137, 284
BstSCI CCNGG 1 cut(s) 362
BstV1I GCAGC 2 cut(s) 287, 395
BstXI CCANNNNNNTGG 1 cut(s) 461
BsuRI GGCC 1 cut(s) 92
BtgZI GCGATG 1 cut(s) 21
BtsIMutI CAGTG 2 cut(s) 258, 304
BveI ACCTGC 1 cut(s) 276
Cac8I GCNNGC 4 cut(s) 94, 133, 222, 273
CaiI CAGNNNCTG 1 cut(s) 47
CfoI GCGC 1 cut(s) 226
CseI GACGC 1 cut(s) 375
Csp6I GTAC 1 cut(s) 309
CviJI RGCY 9 cut(s) 38, 83, 92, 96, 131, 193, 220, 278, 314
CviKI_1 RGCY 9 cut(s) 38, 83, 92, 96, 131, 193, 220, 278, 314
CviQI GTAC 1 cut(s) 309
DdeI CTNAG 5 cut(s) 40, 79, 250, 326, 420
DpnI GATC 2 cut(s) 12, 204
DpnII GATC 2 cut(s) 10, 202
Esp3I CGTCTC 1 cut(s) 393
FaiI YATR 2 cut(s) 306, 434
FblI GTMKAC 1 cut(s) 179
Fnu4HI GCNGC 2 cut(s) 276, 384
Fsp4HI GCNGC 2 cut(s) 276, 384
FspBI CTAG 1 cut(s) 128
GlaI GCGC 1 cut(s) 225
GluI GCNGC 2 cut(s) 276, 384
HaeIII GGCC 1 cut(s) 92
HapII CCGG 1 cut(s) 363
HgaI GACGC 1 cut(s) 375
HhaI GCGC 1 cut(s) 226
Hin6I GCGC 1 cut(s) 224
HinP1I GCGC 1 cut(s) 224
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HpaII CCGG 1 cut(s) 363
HphI GGTGA 1 cut(s) 422
Hpy166II GTNNAC 2 cut(s) 180, 214
Hpy188I TCNGA 2 cut(s) 43, 327
Hpy188III TCNNGA 1 cut(s) 196
Hpy8I GTNNAC 2 cut(s) 180, 214
Hpy99I CGWCG 1 cut(s) 410
HpyAV CCTTC 1 cut(s) 154
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4V TGCA 5 cut(s) 30, 113, 140, 271, 344
HpyF10VI GCNNNNNNNGC 7 cut(s) 44, 89, 102, 119, 128, 137, 284
HpyF3I CTNAG 5 cut(s) 40, 79, 250, 326, 420
HspAI GCGC 1 cut(s) 224
Kzo9I GATC 2 cut(s) 10, 202
LpnPI CCDG 9 cut(s) 106, 108, 117, 234, 237, 281, 310, 360, 376
Lsp1109I GCAGC 2 cut(s) 287, 395
LweI GCATC 2 cut(s) 34, 180
MaeI CTAG 1 cut(s) 128
MaeIII GTNAC 1 cut(s) 410
MalI GATC 2 cut(s) 12, 204
MboI GATC 2 cut(s) 10, 202
MboII GAAGA 1 cut(s) 251
MfeI CAATTG 1 cut(s) 455
MluCI AATT 1 cut(s) 455
MmeI TCCRAC 1 cut(s) 43
MnlI CCTC 4 cut(s) 49, 82, 128, 369
MslI CAYNNNNRTG 1 cut(s) 327
MspI CCGG 1 cut(s) 363
MspR9I CCNGG 1 cut(s) 364
MunI CAATTG 1 cut(s) 455
Mva1269I GAATGC 1 cut(s) 346
MwoI GCNNNNNNNGC 7 cut(s) 44, 89, 102, 119, 128, 137, 284
NciI CCSGG 1 cut(s) 364
NdeII GATC 2 cut(s) 10, 202
NlaIV GGNNCC 2 cut(s) 256, 368
NmuCI GTSAC 1 cut(s) 410
PctI GAATGC 1 cut(s) 346
PkrI GCNGC 2 cut(s) 277, 385
PspN4I GGNNCC 2 cut(s) 256, 368
PstNI CAGNNNCTG 1 cut(s) 47
RsaI GTAC 1 cut(s) 310
RsaNI GTAC 1 cut(s) 309
RseI CAYNNNNRTG 1 cut(s) 327
SatI GCNGC 2 cut(s) 276, 384
Sau3AI GATC 2 cut(s) 10, 202
ScrFI CCNGG 1 cut(s) 364
SetI ASST 8 cut(s) 120, 133, 150, 195, 256, 270, 338, 349
SfaNI GCATC 2 cut(s) 34, 180
SmiMI CAYNNNNRTG 1 cut(s) 327
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
Sse9I AATT 1 cut(s) 455
SspMI CTAG 1 cut(s) 128
StyD4I CCNGG 1 cut(s) 362
TaaI ACNGT 1 cut(s) 410
TasI AATT 1 cut(s) 455
TscAI CASTG 2 cut(s) 265, 304
TseFI GTSAC 1 cut(s) 410
TseI GCWGC 2 cut(s) 275, 383
Tsp45I GTSAC 1 cut(s) 410
TspRI CASTG 2 cut(s) 265, 304
XmiI GTMKAC 1 cut(s) 179
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.