Rroxscaffold_3G00250910

invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002424
Physical Location & Seq
Reverse (-)
44734931 .. 44735404
474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00250910.1

Sequence Viewer

Length: 474 bp
ATGGGTTGCAACGATCTCATCCATCTCTCCTGCAAGAAAGCTGCAGCTAGTGATCCCAATTTGAGCTACAACTTCTGTGTTTCAAGCCTTGAGGCCATCCCCAAAAGCCATGGAGCAGACCTCGAGCAACTTGTGGCCCTTACACTTAACCTCACCATATCCAACGCAACCAGCATCAATTCCACCATTTCGAAGCTTTTGCTCGATAGAAAATTCGACAAGTATGCAAGGGAGTGCTTGCAAGACTGCTTAGAGCTGTATTCGGATGCCGTTCCTACCCTACAAGAAGCTGTTGGTGCCTTTAAGGCCAAGGATTATGGTAAAGCTAACATAGAAATCAGTGCTGCCATGGATGCATCTAGCACTTGTGAAGACGGGTTCATGGAGAAGAAAGGTGAAGTTTATCCATTAAAAAGGGAGAATGATAACTTCAGCCAATTGAACGTCATCTCCTTGCTTTTATCAACACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

157

Amino Acids

17.14

Weight (kDa)

5.01

Isoelectric Point (pI)

32.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 5 - 152 1.5e-28 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 296
AclWI GGATC 1 cut(s) 47
AcsI RAATTY 1 cut(s) 212
AcuI CTGAAG 1 cut(s) 415
AgsI TTSAA 2 cut(s) 84, 442
AloI GAACNNNNNNTCC 2 cut(s) 434, 466
AluBI AGCT 7 cut(s) 41, 47, 66, 196, 256, 290, 326
AluI AGCT 7 cut(s) 41, 47, 66, 196, 256, 290, 326
AlwI GGATC 1 cut(s) 47
Ama87I CYCGRG 1 cut(s) 122
AoxI GGCC 3 cut(s) 93, 135, 306
ApeKI GCWGC 3 cut(s) 41, 44, 344
ApoI RAATTY 1 cut(s) 212
AspS9I GGNCC 1 cut(s) 136
AsuHPI GGTGA 2 cut(s) 145, 407
AsuII TTCGAA 1 cut(s) 191
AvaI CYCGRG 1 cut(s) 122
BanI GGYRCC 1 cut(s) 296
BbsI GAAGAC 1 cut(s) 378
BbvI GCAGC 3 cut(s) 28, 56, 331
BccI CCATC 2 cut(s) 30, 104
BceAI ACGGC 1 cut(s) 254
BcgI CGANNNNNNTGC 4 cut(s) 181, 206, 215, 240
BfaI CTAG 2 cut(s) 48, 360
BfmI CTRYAG 1 cut(s) 42
BglI GCCNNNNNGGC 1 cut(s) 305
BisI GCNGC 3 cut(s) 42, 45, 345
BlsI GCNGC 3 cut(s) 43, 46, 346
BmeT110I CYCGRG 1 cut(s) 122
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 1 cut(s) 298
BmsI GCATC 4 cut(s) 183, 256, 343, 365
BpiI GAAGAC 1 cut(s) 378
BplI GAGNNNNNCTC 2 cut(s) 105, 137
Bpu14I TTCGAA 1 cut(s) 191
BpuEI CTTGAG 1 cut(s) 110
BsaJI CCNNGG 3 cut(s) 109, 309, 348
BsaXI ACNNNNNCTCC 2 cut(s) 434, 464
BseDI CCNNGG 3 cut(s) 109, 309, 348
BseGI GGATG 4 cut(s) 18, 96, 271, 358
BseXI GCAGC 3 cut(s) 28, 56, 331
BshFI GGCC 3 cut(s) 95, 137, 308
BshNI GGYRCC 1 cut(s) 296
BsiHKCI CYCGRG 1 cut(s) 122
BsnI GGCC 3 cut(s) 95, 137, 308
BsoBI CYCGRG 1 cut(s) 122
Bsp119I TTCGAA 1 cut(s) 191
Bsp143I GATC 2 cut(s) 13, 52
Bsp19I CCATGG 2 cut(s) 109, 348
BspANI GGCC 3 cut(s) 95, 137, 308
BspLI GGNNCC 1 cut(s) 298
BspMAI CTGCAG 1 cut(s) 46
BspPI GGATC 1 cut(s) 47
BspT104I TTCGAA 1 cut(s) 191
BspT107I GGYRCC 1 cut(s) 296
BssECI CCNNGG 3 cut(s) 109, 309, 348
BssMI GATC 2 cut(s) 13, 52
BssT1I CCWWGG 3 cut(s) 109, 309, 348
BstBI TTCGAA 1 cut(s) 191
BstC8I GCNNGC 1 cut(s) 239
BstDEI CTNAG 1 cut(s) 250
BstDSI CCRYGG 2 cut(s) 109, 348
BstF5I GGATG 4 cut(s) 18, 96, 271, 358
BstKTI GATC 2 cut(s) 16, 55
BstMBI GATC 2 cut(s) 13, 52
BstMWI GCNNNNNNNGC 3 cut(s) 296, 305, 353
BstSFI CTRYAG 1 cut(s) 42
BstV1I GCAGC 3 cut(s) 28, 56, 331
BstV2I GAAGAC 1 cut(s) 378
BsuRI GGCC 3 cut(s) 95, 137, 308
BtgI CCRYGG 2 cut(s) 109, 348
BtsCI GGATG 4 cut(s) 18, 96, 271, 358
BtsIMutI CAGTG 1 cut(s) 346
Cac8I GCNNGC 1 cut(s) 239
Cfr13I GGNCC 1 cut(s) 136
CviAII CATG 3 cut(s) 110, 349, 382
DdeI CTNAG 1 cut(s) 250
DpnI GATC 2 cut(s) 15, 54
DpnII GATC 2 cut(s) 13, 52
Eco130I CCWWGG 3 cut(s) 109, 309, 348
Eco57I CTGAAG 1 cut(s) 415
Eco88I CYCGRG 1 cut(s) 122
EcoT14I CCWWGG 3 cut(s) 109, 309, 348
EcoT22I ATGCAT 1 cut(s) 358
ErhI CCWWGG 3 cut(s) 109, 309, 348
FaeI CATG 3 cut(s) 113, 352, 385
FaiI YATR 7 cut(s) 111, 158, 225, 318, 332, 350, 383
FatI CATG 3 cut(s) 109, 348, 381
Fnu4HI GCNGC 3 cut(s) 42, 45, 345
FokI GGATG 4 cut(s) 5, 83, 278, 365
Fsp4HI GCNGC 3 cut(s) 42, 45, 345
FspBI CTAG 2 cut(s) 48, 360
GluI GCNGC 3 cut(s) 42, 45, 345
HaeIII GGCC 3 cut(s) 95, 137, 308
Hin1II CATG 3 cut(s) 113, 352, 385
HindIII AAGCTT 1 cut(s) 194
HphI GGTGA 2 cut(s) 145, 407
Hpy188I TCNGA 1 cut(s) 265
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 6 cut(s) 9, 33, 44, 227, 241, 356
HpyF10VI GCNNNNNNNGC 3 cut(s) 296, 305, 353
HpyF3I CTNAG 1 cut(s) 250
HpySE526I ACGT 1 cut(s) 444
Hsp92II CATG 3 cut(s) 113, 352, 385
Kzo9I GATC 2 cut(s) 13, 52
LmnI GCTCC 1 cut(s) 113
LpnPI CCDG 2 cut(s) 43, 184
Lsp1109I GCAGC 3 cut(s) 28, 56, 331
LweI GCATC 4 cut(s) 183, 256, 343, 365
MaeI CTAG 2 cut(s) 48, 360
MaeII ACGT 1 cut(s) 444
MalI GATC 2 cut(s) 15, 54
MboI GATC 2 cut(s) 13, 52
MboII GAAGA 2 cut(s) 383, 400
MfeI CAATTG 1 cut(s) 437
MluCI AATT 4 cut(s) 58, 178, 212, 437
MmeI TCCRAC 1 cut(s) 186
MnlI CCTC 3 cut(s) 85, 131, 161
Mph1103I ATGCAT 1 cut(s) 358
MseI TTAA 3 cut(s) 147, 303, 410
MunI CAATTG 1 cut(s) 437
MwoI GCNNNNNNNGC 3 cut(s) 296, 305, 353
NcoI CCATGG 2 cut(s) 109, 348
NdeII GATC 2 cut(s) 13, 52
NlaIII CATG 3 cut(s) 113, 352, 385
NlaIV GGNNCC 1 cut(s) 298
NsiI ATGCAT 1 cut(s) 358
NspV TTCGAA 1 cut(s) 191
PaeR7I CTCGAG 1 cut(s) 122
PkrI GCNGC 3 cut(s) 43, 46, 346
PspN4I GGNNCC 1 cut(s) 298
PspPI GGNCC 1 cut(s) 136
PspXI VCTCGAGB 1 cut(s) 122
PstI CTGCAG 1 cut(s) 46
SaqAI TTAA 3 cut(s) 147, 303, 410
SatI GCNGC 3 cut(s) 42, 45, 345
Sau3AI GATC 2 cut(s) 13, 52
Sau96I GGNCC 1 cut(s) 136
SfaNI GCATC 4 cut(s) 183, 256, 343, 365
SfcI CTRYAG 1 cut(s) 42
Sfr274I CTCGAG 1 cut(s) 122
SfuI TTCGAA 1 cut(s) 191
SlaI CTCGAG 1 cut(s) 122
SmlI CTYRAG 2 cut(s) 89, 122
SmoI CTYRAG 2 cut(s) 89, 122
Sse9I AATT 4 cut(s) 58, 178, 212, 437
SspMI CTAG 2 cut(s) 48, 360
StyI CCWWGG 3 cut(s) 109, 309, 348
TaiI ACGT 1 cut(s) 447
TaqI TCGA 4 cut(s) 123, 191, 204, 216
TasI AATT 4 cut(s) 58, 178, 212, 437
Tru1I TTAA 3 cut(s) 147, 303, 410
Tru9I TTAA 3 cut(s) 147, 303, 410
TscAI CASTG 1 cut(s) 346
TseI GCWGC 3 cut(s) 41, 44, 344
TspDTI ATGAA 1 cut(s) 370
TspRI CASTG 1 cut(s) 346
XapI RAATTY 1 cut(s) 212
XhoI CTCGAG 1 cut(s) 122
XspI CTAG 2 cut(s) 48, 360
Zsp2I ATGCAT 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.