FvH4_5g20930

invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002424
Physical Location & Seq
Forward (+)
12578790 .. 12579866
1077 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20930.t1

Sequence Viewer

Length: 522 bp
ATGAGGTCTCTCTTCCTTACCATCCTTTTTGTCGTGCTATGTCACAGCACAATGGGCGATGATCTCGTCCAACAAGCTTGCAAGAAAGCCGCAGACGGTGACAAAGCCTTGAACTACAACTTCTGTGTCTCAACCCTCGAGGCCAGCCCGGGAAGCAAGGGTGCAGATCTTGCAGGACTAGTCGGAATCGCACTTGACCTAACCACAAAGAAGGCCACGAGCATCAAGTCCACCATAACAAAGCTTCAGAGCGATCCAAAAGTTGACAAGTCTGCGCTACAAGACTGCGCAGAACTTTTTTCGGATGCCCTTGATAGTCTGGGACAAGCGACCAGTGCATTCAAGTCCAAAGATTATGGCTCAGCTCTTACACAGCTCAGCGCCTCCTTGGATGCACCTGATACTTGTGAAACTGGGTTCAAAGAGAAGAAAGCAGTGTCACCTTTGAGCAAAGACGACGCTGACTGCACTCAGTTGAACATCATATGTCTTGCTTTTATCAACCAGTTGCGCGGGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

174

Amino Acids

18.24

Weight (kDa)

5.1

Isoelectric Point (pI)

25.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 21 - 165 7.2e-28 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 137
Acc16I TGCGCA 1 cut(s) 289
AccII CGCG 1 cut(s) 513
AciI CCGC 2 cut(s) 90, 513
AclWI GGATC 1 cut(s) 248
AcuI CTGAAG 1 cut(s) 230
AgsI TTSAA 4 cut(s) 112, 343, 421, 478
AhlI ACTAGT 1 cut(s) 178
AluBI AGCT 4 cut(s) 77, 244, 365, 376
AluI AGCT 4 cut(s) 77, 244, 365, 376
Alw26I GTCTC 2 cut(s) 12, 133
AlwI GGATC 1 cut(s) 248
Ama87I CYCGRG 2 cut(s) 137, 148
AoxI GGCC 2 cut(s) 141, 213
AspLEI GCGC 4 cut(s) 277, 290, 383, 513
AsuC2I CCSGG 2 cut(s) 149, 150
AsuHPI GGTGA 2 cut(s) 110, 432
AvaI CYCGRG 2 cut(s) 137, 148
BauI CACGAG 1 cut(s) 217
BccI CCATC 1 cut(s) 29
BcnI CCSGG 2 cut(s) 149, 150
BcoDI GTCTC 2 cut(s) 12, 133
BcuI ACTAGT 1 cut(s) 178
BfaI CTAG 1 cut(s) 179
BfoI RGCGCY 1 cut(s) 384
BglII AGATCT 1 cut(s) 166
BisI GCNGC 1 cut(s) 90
BlpI GCTNAGC 2 cut(s) 361, 377
BlsI GCNGC 1 cut(s) 91
Bme1390I CCNGG 2 cut(s) 149, 150
BmeT110I CYCGRG 2 cut(s) 137, 148
BmrFI CCNGG 2 cut(s) 149, 150
BmrI ACTGGG 1 cut(s) 423
BmsI GCATC 3 cut(s) 231, 295, 382
BmuI ACTGGG 1 cut(s) 423
Bpu1102I GCTNAGC 2 cut(s) 361, 377
BpuMI CCSGG 2 cut(s) 149, 150
BsaI GGTCTC 1 cut(s) 12
BsaJI CCNNGG 2 cut(s) 148, 387
Bse1I ACTGG 3 cut(s) 333, 418, 505
BseDI CCNNGG 2 cut(s) 148, 387
BseGI GGATG 3 cut(s) 21, 310, 397
BseMII CTCAG 3 cut(s) 375, 391, 485
BseNI ACTGG 3 cut(s) 333, 418, 505
BsgI GTGCAG 2 cut(s) 183, 451
Bsh1236I CGCG 1 cut(s) 513
BshFI GGCC 2 cut(s) 143, 215
BsiHKCI CYCGRG 2 cut(s) 137, 148
BsiSI CCGG 1 cut(s) 149
BslFI GGGAC 1 cut(s) 336
BsmAI GTCTC 2 cut(s) 12, 133
BsmFI GGGAC 1 cut(s) 336
BsmI GAATGC 1 cut(s) 338
BsnI GGCC 2 cut(s) 143, 215
Bso31I GGTCTC 1 cut(s) 12
BsoBI CYCGRG 2 cut(s) 137, 148
Bsp143I GATC 3 cut(s) 61, 166, 253
Bsp1720I GCTNAGC 2 cut(s) 361, 377
BspACI CCGC 2 cut(s) 90, 513
BspANI GGCC 2 cut(s) 143, 215
BspCNI CTCAG 3 cut(s) 374, 390, 484
BspFNI CGCG 1 cut(s) 513
BspPI GGATC 1 cut(s) 248
BspTNI GGTCTC 1 cut(s) 12
BsrI ACTGG 3 cut(s) 333, 418, 505
BssECI CCNNGG 2 cut(s) 148, 387
BssMI GATC 3 cut(s) 61, 166, 253
BssSI CACGAG 1 cut(s) 217
BssT1I CCWWGG 1 cut(s) 387
Bst2BI CACGAG 1 cut(s) 217
Bst4CI ACNGT 1 cut(s) 98
Bst6I CTCTTC 1 cut(s) 17
BstAPI GCANNNNNTGC 1 cut(s) 170
BstC8I GCNNGC 2 cut(s) 79, 145
BstDEI CTNAG 3 cut(s) 361, 377, 471
BstF5I GGATG 3 cut(s) 21, 310, 397
BstFNI CGCG 1 cut(s) 513
BstH2I RGCGCY 1 cut(s) 384
BstHHI GCGC 4 cut(s) 277, 290, 383, 513
BstKTI GATC 3 cut(s) 64, 169, 256
BstMAI GTCTC 2 cut(s) 12, 133
BstMBI GATC 3 cut(s) 61, 166, 253
BstMWI GCNNNNNNNGC 4 cut(s) 54, 153, 170, 335
BstSCI CCNGG 2 cut(s) 147, 148
BstUI CGCG 1 cut(s) 513
BstX2I RGATCY 1 cut(s) 166
BstYI RGATCY 1 cut(s) 166
BsuRI GGCC 2 cut(s) 143, 215
BtgZI GCGATG 1 cut(s) 72
BtsCI GGATG 3 cut(s) 21, 310, 397
BtsI GCAGTG 1 cut(s) 441
BtsIMutI CAGTG 2 cut(s) 340, 441
Cac8I GCNNGC 2 cut(s) 79, 145
CfoI GCGC 4 cut(s) 277, 290, 383, 513
Cfr9I CCCGGG 1 cut(s) 148
CseI GACGC 1 cut(s) 467
DdeI CTNAG 3 cut(s) 361, 377, 471
DpnI GATC 3 cut(s) 63, 168, 255
DpnII GATC 3 cut(s) 61, 166, 253
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Eco130I CCWWGG 1 cut(s) 387
Eco31I GGTCTC 1 cut(s) 12
Eco57I CTGAAG 1 cut(s) 230
Eco88I CYCGRG 2 cut(s) 137, 148
EcoT14I CCWWGG 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 387
FaiI YATR 6 cut(s) 40, 236, 357, 485, 487, 520
FaqI GGGAC 1 cut(s) 336
FauI CCCGC 1 cut(s) 506
FauNDI CATATG 1 cut(s) 485
Fnu4HI GCNGC 1 cut(s) 90
FokI GGATG 3 cut(s) 8, 317, 404
Fsp4HI GCNGC 1 cut(s) 90
FspBI CTAG 1 cut(s) 179
FspI TGCGCA 1 cut(s) 289
GlaI GCGC 4 cut(s) 276, 289, 382, 512
GluI GCNGC 1 cut(s) 90
HaeII RGCGCY 1 cut(s) 384
HaeIII GGCC 2 cut(s) 143, 215
HapII CCGG 1 cut(s) 149
HgaI GACGC 1 cut(s) 467
HhaI GCGC 4 cut(s) 277, 290, 383, 513
Hin6I GCGC 4 cut(s) 275, 288, 381, 511
HinP1I GCGC 4 cut(s) 275, 288, 381, 511
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HindIII AAGCTT 2 cut(s) 75, 242
HinfI GANTC 1 cut(s) 186
HpaII CCGG 1 cut(s) 149
HphI GGTGA 2 cut(s) 110, 432
Hpy166II GTNNAC 2 cut(s) 231, 265
Hpy188I TCNGA 3 cut(s) 185, 249, 304
Hpy8I GTNNAC 2 cut(s) 231, 265
Hpy99I CGWCG 1 cut(s) 461
HpyAV CCTTC 1 cut(s) 205
HpyCH4III ACNGT 1 cut(s) 98
HpyCH4V TGCA 6 cut(s) 81, 164, 173, 338, 395, 468
HpyF10VI GCNNNNNNNGC 4 cut(s) 54, 153, 170, 335
HpyF3I CTNAG 3 cut(s) 361, 377, 471
HspAI GCGC 4 cut(s) 275, 288, 381, 511
Kzo9I GATC 3 cut(s) 61, 166, 253
LpnPI CCDG 8 cut(s) 157, 159, 162, 305, 346, 399, 411, 518
LweI GCATC 3 cut(s) 231, 295, 382
MaeI CTAG 1 cut(s) 179
MaeIII GTNAC 3 cut(s) 41, 98, 438
MalI GATC 3 cut(s) 63, 168, 255
MboI GATC 3 cut(s) 61, 166, 253
MboII GAAGA 2 cut(s) 4, 439
MflI RGATCY 1 cut(s) 166
MmeI TCCRAC 2 cut(s) 94, 163
MnlI CCTC 3 cut(s) 133, 146, 394
MspI CCGG 1 cut(s) 149
MspR9I CCNGG 2 cut(s) 149, 150
Mva1269I GAATGC 1 cut(s) 338
MvnI CGCG 1 cut(s) 513
MwoI GCNNNNNNNGC 4 cut(s) 54, 153, 170, 335
NciI CCSGG 2 cut(s) 149, 150
NdeI CATATG 1 cut(s) 485
NdeII GATC 3 cut(s) 61, 166, 253
NmuCI GTSAC 3 cut(s) 41, 98, 438
NsbI TGCGCA 1 cut(s) 289
PaeR7I CTCGAG 1 cut(s) 137
PctI GAATGC 1 cut(s) 338
PfeI GAWTC 1 cut(s) 186
PkrI GCNGC 1 cut(s) 91
PspXI VCTCGAGB 1 cut(s) 137
PsuI RGATCY 1 cut(s) 166
SatI GCNGC 1 cut(s) 90
Sau3AI GATC 3 cut(s) 61, 166, 253
ScrFI CCNGG 2 cut(s) 149, 150
SetI ASST 8 cut(s) 8, 79, 201, 246, 367, 378, 400, 445
SfaNI GCATC 3 cut(s) 231, 295, 382
Sfr274I CTCGAG 1 cut(s) 137
SlaI CTCGAG 1 cut(s) 137
SmaI CCCGGG 1 cut(s) 150
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
SpeI ACTAGT 1 cut(s) 178
SsiI CCGC 2 cut(s) 90, 513
SspMI CTAG 1 cut(s) 179
StyD4I CCNGG 2 cut(s) 147, 148
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 1 cut(s) 98
TaqI TCGA 1 cut(s) 138
TauI GCSGC 1 cut(s) 92
TfiI GAWTC 1 cut(s) 186
TscAI CASTG 2 cut(s) 340, 441
TseFI GTSAC 3 cut(s) 41, 98, 438
Tsp45I GTSAC 3 cut(s) 41, 98, 438
TspMI CCCGGG 1 cut(s) 148
TspRI CASTG 2 cut(s) 340, 441
XhoI CTCGAG 1 cut(s) 137
XmaI CCCGGG 1 cut(s) 148
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.