FvH4_5g20940

invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002424
Physical Location & Seq
Forward (+)
12592701 .. 12593591
891 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20940.t1

Sequence Viewer

Length: 555 bp
ATGTTTGTGGAAAGAATGAGGTACTTCTTCTTTATCACTTTTCTCGTGCTCTTTCGCAACACAATGGGTTCCAACGATCTCATCCGACTCTCCTGCAAGAAAGCTGCAGCTAGTGATCCCAATTTGCGCTACAAGTTCTGTGTTTCAAGCCTTGAGGCCATCCCCCAAAGCCATGGAGCAGACCTTGAGCAACTTGTAGCCATTACACTTAACCTCACCATATCCAACGCGACCAGCATCACTTCCACCATTTCGAAGCTTTTGGTCGAGAAACAATTCGACAAGTATGCAAAGGAGTGCTTGGAAGACTGTTTAGAGCTGTATTCGGATGCCGATCTTACCCTACAGGACGCTGTGGGTGCCTTGAAGGCCAAGGATTATGGTAAAGCTAACATAGAAATCAGTTCTGCCATGGATGCATCTAGCACTTGTGAAGATGGGTTCTTGGAGAAGAAAGGTGAAGTTTATCCATTGAAAAGGGAGAATGATCACTTCTGCCAATTGAACATCATCTCTCTTGCTTTTATCAACACATTAGCTCAGGATCATAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

185

Amino Acids

20.64

Weight (kDa)

5.24

Isoelectric Point (pI)

36.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 26 - 174 2.1e-26 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 359
AccII CGCG 1 cut(s) 230
AclWI GGATC 2 cut(s) 110, 552
AfaI GTAC 1 cut(s) 23
AgsI TTSAA 4 cut(s) 147, 367, 475, 505
AluBI AGCT 6 cut(s) 104, 110, 259, 319, 389, 539
AluI AGCT 6 cut(s) 104, 110, 259, 319, 389, 539
Alw21I GWGCWC 1 cut(s) 51
AlwI GGATC 2 cut(s) 110, 552
AoxI GGCC 2 cut(s) 156, 369
ApeKI GCWGC 2 cut(s) 104, 107
Asp700I GAANNNNTTC 1 cut(s) 275
AspLEI GCGC 1 cut(s) 129
AsuHPI GGTGA 2 cut(s) 208, 470
AsuII TTCGAA 1 cut(s) 254
BanI GGYRCC 1 cut(s) 359
BauI CACGAG 1 cut(s) 44
BbsI GAAGAC 1 cut(s) 312
Bbv12I GWGCWC 1 cut(s) 51
BbvI GCAGC 2 cut(s) 91, 119
BccI CCATC 2 cut(s) 167, 431
BcgI CGANNNNNNTGC 4 cut(s) 75, 109, 269, 303
BclI TGATCA 1 cut(s) 487
BfaI CTAG 2 cut(s) 111, 423
BfmI CTRYAG 2 cut(s) 105, 344
BglI GCCNNNNNGGC 1 cut(s) 368
BisI GCNGC 2 cut(s) 105, 108
BlsI GCNGC 2 cut(s) 106, 109
BmiI GGNNCC 2 cut(s) 70, 361
BmsI GCATC 4 cut(s) 246, 319, 406, 428
BpiI GAAGAC 1 cut(s) 312
Bpu10I CCTNAGC 1 cut(s) 540
Bpu14I TTCGAA 1 cut(s) 254
BpuEI CTTGAG 2 cut(s) 173, 206
BsaBI GATNNNNATC 1 cut(s) 333
BsaJI CCNNGG 3 cut(s) 172, 372, 411
Bse8I GATNNNNATC 1 cut(s) 333
BseDI CCNNGG 3 cut(s) 172, 372, 411
BseGI GGATG 4 cut(s) 81, 159, 334, 421
BseJI GATNNNNATC 1 cut(s) 333
BseMII CTCAG 1 cut(s) 554
BseXI GCAGC 2 cut(s) 91, 119
Bsh1236I CGCG 1 cut(s) 230
BshFI GGCC 2 cut(s) 158, 371
BshNI GGYRCC 1 cut(s) 359
BsiHKAI GWGCWC 1 cut(s) 51
BsnI GGCC 2 cut(s) 158, 371
Bsp119I TTCGAA 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 51
Bsp143I GATC 5 cut(s) 76, 115, 334, 487, 544
Bsp19I CCATGG 2 cut(s) 172, 411
BspANI GGCC 2 cut(s) 158, 371
BspCNI CTCAG 1 cut(s) 553
BspFNI CGCG 1 cut(s) 230
BspLI GGNNCC 2 cut(s) 70, 361
BspMAI CTGCAG 1 cut(s) 109
BspPI GGATC 2 cut(s) 110, 552
BspT104I TTCGAA 1 cut(s) 254
BspT107I GGYRCC 1 cut(s) 359
BssECI CCNNGG 3 cut(s) 172, 372, 411
BssMI GATC 5 cut(s) 76, 115, 334, 487, 544
BssSI CACGAG 1 cut(s) 44
BssT1I CCWWGG 3 cut(s) 172, 372, 411
Bst2BI CACGAG 1 cut(s) 44
Bst4CI ACNGT 1 cut(s) 311
BstBI TTCGAA 1 cut(s) 254
BstDEI CTNAG 1 cut(s) 540
BstDSI CCRYGG 2 cut(s) 172, 411
BstF5I GGATG 4 cut(s) 81, 159, 334, 421
BstFNI CGCG 1 cut(s) 230
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 5 cut(s) 79, 118, 337, 490, 547
BstMBI GATC 5 cut(s) 76, 115, 334, 487, 544
BstMWI GCNNNNNNNGC 3 cut(s) 359, 368, 416
BstSFI CTRYAG 2 cut(s) 105, 344
BstUI CGCG 1 cut(s) 230
BstV1I GCAGC 2 cut(s) 91, 119
BstV2I GAAGAC 1 cut(s) 312
BstXI CCANNNNNNTGG 1 cut(s) 173
BsuRI GGCC 2 cut(s) 158, 371
BtgI CCRYGG 2 cut(s) 172, 411
BtsCI GGATG 4 cut(s) 81, 159, 334, 421
CfoI GCGC 1 cut(s) 129
CseI GACGC 1 cut(s) 359
Csp6I GTAC 1 cut(s) 22
CviAII CATG 2 cut(s) 173, 412
CviQI GTAC 1 cut(s) 22
DdeI CTNAG 1 cut(s) 540
DpnI GATC 5 cut(s) 78, 117, 336, 489, 546
DpnII GATC 5 cut(s) 76, 115, 334, 487, 544
Eco130I CCWWGG 3 cut(s) 172, 372, 411
EcoT14I CCWWGG 3 cut(s) 172, 372, 411
EcoT22I ATGCAT 1 cut(s) 421
ErhI CCWWGG 3 cut(s) 172, 372, 411
FaeI CATG 2 cut(s) 176, 415
FaiI YATR 7 cut(s) 174, 221, 288, 381, 395, 413, 549
FalI AAGNNNNNCTT 2 cut(s) 284, 316
FatI CATG 2 cut(s) 172, 411
FbaI TGATCA 1 cut(s) 487
Fnu4HI GCNGC 2 cut(s) 105, 108
FokI GGATG 4 cut(s) 68, 146, 341, 428
Fsp4HI GCNGC 2 cut(s) 105, 108
FspBI CTAG 2 cut(s) 111, 423
GlaI GCGC 1 cut(s) 128
GluI GCNGC 2 cut(s) 105, 108
HaeIII GGCC 2 cut(s) 158, 371
HgaI GACGC 1 cut(s) 359
HhaI GCGC 1 cut(s) 129
Hin1II CATG 2 cut(s) 176, 415
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 1 cut(s) 87
HphI GGTGA 2 cut(s) 208, 470
Hpy188I TCNGA 2 cut(s) 86, 328
Hpy188III TCNNGA 2 cut(s) 268, 542
HpyAV CCTTC 1 cut(s) 361
HpyCH4III ACNGT 1 cut(s) 311
HpyCH4V TGCA 4 cut(s) 96, 107, 290, 419
HpyF10VI GCNNNNNNNGC 3 cut(s) 359, 368, 416
HpyF3I CTNAG 1 cut(s) 540
Hsp92II CATG 2 cut(s) 176, 415
HspAI GCGC 1 cut(s) 127
Ksp22I TGATCA 1 cut(s) 487
Kzo9I GATC 5 cut(s) 76, 115, 334, 487, 544
LmnI GCTCC 1 cut(s) 176
LpnPI CCDG 4 cut(s) 106, 247, 332, 527
Lsp1109I GCAGC 2 cut(s) 91, 119
LweI GCATC 4 cut(s) 246, 319, 406, 428
MaeI CTAG 2 cut(s) 111, 423
MalI GATC 5 cut(s) 78, 117, 336, 489, 546
MboI GATC 5 cut(s) 76, 115, 334, 487, 544
MboII GAAGA 4 cut(s) 19, 317, 446, 463
MfeI CAATTG 1 cut(s) 500
MhlI GDGCHC 1 cut(s) 51
MluCI AATT 3 cut(s) 121, 275, 500
MlyI GAGTC 1 cut(s) 81
MmeI TCCRAC 3 cut(s) 96, 109, 249
MnlI CCTC 3 cut(s) 12, 148, 224
Mph1103I ATGCAT 1 cut(s) 421
MroXI GAANNNNTTC 1 cut(s) 275
MseI TTAA 1 cut(s) 210
MunI CAATTG 1 cut(s) 500
MvnI CGCG 1 cut(s) 230
MwoI GCNNNNNNNGC 3 cut(s) 359, 368, 416
NcoI CCATGG 2 cut(s) 172, 411
NdeII GATC 5 cut(s) 76, 115, 334, 487, 544
NlaIII CATG 2 cut(s) 176, 415
NlaIV GGNNCC 2 cut(s) 70, 361
NsiI ATGCAT 1 cut(s) 421
NspV TTCGAA 1 cut(s) 254
PdmI GAANNNNTTC 1 cut(s) 275
PkrI GCNGC 2 cut(s) 106, 109
PleI GAGTC 1 cut(s) 81
PpsI GAGTC 1 cut(s) 81
PspN4I GGNNCC 2 cut(s) 70, 361
PstI CTGCAG 1 cut(s) 109
RsaI GTAC 1 cut(s) 23
RsaNI GTAC 1 cut(s) 22
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 2 cut(s) 105, 108
Sau3AI GATC 5 cut(s) 76, 115, 334, 487, 544
SchI GAGTC 1 cut(s) 81
SduI GDGCHC 1 cut(s) 51
SfaNI GCATC 4 cut(s) 246, 319, 406, 428
SfcI CTRYAG 2 cut(s) 105, 344
SfuI TTCGAA 1 cut(s) 254
SmlI CTYRAG 2 cut(s) 152, 185
SmoI CTYRAG 2 cut(s) 152, 185
Sse9I AATT 3 cut(s) 121, 275, 500
SspMI CTAG 2 cut(s) 111, 423
StyI CCWWGG 3 cut(s) 172, 372, 411
TaaI ACNGT 1 cut(s) 311
TaqI TCGA 3 cut(s) 254, 267, 279
TasI AATT 3 cut(s) 121, 275, 500
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TseI GCWGC 2 cut(s) 104, 107
XmnI GAANNNNTTC 1 cut(s) 275
XspI CTAG 2 cut(s) 111, 423
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.