AT5G46940

invertase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002424
Physical Location & Seq
Reverse (-)
19058031 .. 19058814
784 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46940.1

Sequence Viewer

Length: 531 bp
ATGAAATTTTTGCTTTACCTTGTTACGTTCTTTGTTCTCTCAAACGGTTTGGCAAACGGACAAACTTTGATTCGAAATTCTTGCAAGAAAGCTACAGCTACAAGCCCGAAGTTCAAATACAATCTATGTGTCACGTCTCTCGAAACCAATCCGCAGGCCAAAACCGCAAAAGATCTCGCCGGATTAGTCATGGCATCGACGAAAAACGCCGTGACCAAAGCGACGACCTTGAAAGGAACTGTTGACAAGATTATCAAGGGGAAGAAAGTTAACAAGATGACTGCAATGCCGTTACGTGATTGCCTTCAGCTTTATACCGATGCTATTGGTTCTTTAAACGAAGCTTTAGCGGGCGTTAAATCGCGTAATTACCCAACCGTTAAAACGGTTCTGAGTGCTGCAATGGATACACCAAGTACTTGTGAAACTGGATTCAAAGAAAGAAAAGCGCCATCTCCGGTTACGAAAGAGAACGATAATTTGTATCAGATGATTCTGATTCCTCTAGCTTTTACAAATATGTTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

176

Amino Acids

19.16

Weight (kDa)

9.76

Isoelectric Point (pI)

21.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 23 - 170 1.9e-29 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 364
AciI CCGC 3 cut(s) 152, 165, 350
AcsI RAATTY 2 cut(s) 5, 76
AcuI CTGAAG 1 cut(s) 290
AfaI GTAC 1 cut(s) 418
AgsI TTSAA 4 cut(s) 115, 232, 436, 526
AjiI CACGTC 1 cut(s) 135
AluBI AGCT 5 cut(s) 92, 98, 310, 344, 509
AluI AGCT 5 cut(s) 92, 98, 310, 344, 509
Alw26I GTCTC 1 cut(s) 141
AoxI GGCC 1 cut(s) 156
ApeKI GCWGC 1 cut(s) 398
ApoI RAATTY 2 cut(s) 5, 76
AspLEI GCGC 1 cut(s) 451
AsuII TTCGAA 1 cut(s) 73
BarI GAAGNNNNNNTAC 2 cut(s) 101, 133
BbvI GCAGC 1 cut(s) 385
BccI CCATC 1 cut(s) 460
BceAI ACGGC 2 cut(s) 194, 274
BcgI CGANNNNNNTGC 2 cut(s) 63, 97
BciVI GTATCC 1 cut(s) 400
BcoDI GTCTC 1 cut(s) 141
BfaI CTAG 1 cut(s) 506
BfmI CTRYAG 1 cut(s) 93
BfoI RGCGCY 1 cut(s) 452
BfuI GTATCC 1 cut(s) 400
BglII AGATCT 1 cut(s) 172
BisI GCNGC 1 cut(s) 399
BlsI GCNGC 1 cut(s) 400
BmcAI AGTACT 1 cut(s) 418
BmgBI CACGTC 1 cut(s) 135
BmsI GCATC 2 cut(s) 203, 310
Bpu14I TTCGAA 1 cut(s) 73
BsaAI YACGTR 1 cut(s) 296
BsaWI WCCGGW 1 cut(s) 457
Bse1I ACTGG 1 cut(s) 433
Bse3DI GCAATG 2 cut(s) 291, 408
BseMI GCAATG 2 cut(s) 291, 408
BseMII CTCAG 1 cut(s) 383
BseNI ACTGG 1 cut(s) 433
BseXI GCAGC 1 cut(s) 385
Bsh1236I CGCG 1 cut(s) 364
BshFI GGCC 1 cut(s) 158
BsiSI CCGG 2 cut(s) 180, 458
BsmAI GTCTC 1 cut(s) 141
BsmBI CGTCTC 1 cut(s) 141
BsnI GGCC 1 cut(s) 158
Bsp119I TTCGAA 1 cut(s) 73
Bsp143I GATC 1 cut(s) 172
BspACI CCGC 3 cut(s) 152, 165, 350
BspANI GGCC 1 cut(s) 158
BspCNI CTCAG 1 cut(s) 384
BspFNI CGCG 1 cut(s) 364
BspT104I TTCGAA 1 cut(s) 73
BsrDI GCAATG 2 cut(s) 291, 408
BsrI ACTGG 1 cut(s) 433
BssMI GATC 1 cut(s) 172
Bst4CI ACNGT 4 cut(s) 47, 241, 379, 388
BstBAI YACGTR 1 cut(s) 296
BstBI TTCGAA 1 cut(s) 73
BstC8I GCNNGC 2 cut(s) 156, 352
BstDEI CTNAG 1 cut(s) 392
BstFNI CGCG 1 cut(s) 364
BstH2I RGCGCY 1 cut(s) 452
BstHHI GCGC 1 cut(s) 451
BstKTI GATC 1 cut(s) 175
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 1 cut(s) 172
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstSFI CTRYAG 1 cut(s) 93
BstUI CGCG 1 cut(s) 364
BstV1I GCAGC 1 cut(s) 385
BstX2I RGATCY 1 cut(s) 172
BstYI RGATCY 1 cut(s) 172
BsuI GTATCC 1 cut(s) 400
BsuRI GGCC 1 cut(s) 158
BtrI CACGTC 1 cut(s) 135
Cac8I GCNNGC 2 cut(s) 156, 352
CfoI GCGC 1 cut(s) 451
Csp6I GTAC 1 cut(s) 417
CviAII CATG 1 cut(s) 190
CviJI RGCY 7 cut(s) 92, 98, 105, 158, 310, 344, 509
CviKI_1 RGCY 7 cut(s) 92, 98, 105, 158, 310, 344, 509
CviQI GTAC 1 cut(s) 417
DdeI CTNAG 1 cut(s) 392
DpnI GATC 1 cut(s) 174
DpnII GATC 1 cut(s) 172
DraI TTTAAA 1 cut(s) 336
Eco57I CTGAAG 1 cut(s) 290
Esp3I CGTCTC 1 cut(s) 141
FaeI CATG 1 cut(s) 193
FaiI YATR 4 cut(s) 127, 191, 315, 521
FatI CATG 1 cut(s) 189
FauI CCCGC 1 cut(s) 343
Fnu4HI GCNGC 1 cut(s) 399
Fsp4HI GCNGC 1 cut(s) 399
FspBI CTAG 1 cut(s) 506
GlaI GCGC 1 cut(s) 450
GluI GCNGC 1 cut(s) 399
HaeII RGCGCY 1 cut(s) 452
HaeIII GGCC 1 cut(s) 158
HapII CCGG 2 cut(s) 180, 458
HhaI GCGC 1 cut(s) 451
Hin1II CATG 1 cut(s) 193
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HincII GTYRAC 2 cut(s) 244, 271
HindII GTYRAC 2 cut(s) 244, 271
HindIII AAGCTT 1 cut(s) 342
HinfI GANTC 4 cut(s) 70, 432, 493, 499
HpaI GTTAAC 1 cut(s) 271
HpaII CCGG 2 cut(s) 180, 458
Hpy166II GTNNAC 2 cut(s) 244, 271
Hpy188I TCNGA 3 cut(s) 393, 489, 498
Hpy188III TCNNGA 1 cut(s) 140
Hpy8I GTNNAC 2 cut(s) 244, 271
Hpy99I CGWCG 2 cut(s) 202, 226
HpyAV CCTTC 1 cut(s) 314
HpyCH4III ACNGT 4 cut(s) 47, 241, 379, 388
HpyCH4IV ACGT 3 cut(s) 26, 134, 295
HpyCH4V TGCA 3 cut(s) 84, 284, 401
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpyF3I CTNAG 1 cut(s) 392
HpySE526I ACGT 3 cut(s) 26, 134, 295
Hsp92II CATG 1 cut(s) 193
HspAI GCGC 1 cut(s) 449
KspAI GTTAAC 1 cut(s) 271
Kzo9I GATC 1 cut(s) 172
LpnPI CCDG 4 cut(s) 140, 193, 414, 471
Lsp1109I GCAGC 1 cut(s) 385
LweI GCATC 2 cut(s) 203, 310
MaeI CTAG 1 cut(s) 506
MaeII ACGT 3 cut(s) 26, 134, 295
MaeIII GTNAC 5 cut(s) 22, 130, 211, 291, 460
MalI GATC 1 cut(s) 174
MboI GATC 1 cut(s) 172
MboII GAAGA 1 cut(s) 274
MflI RGATCY 1 cut(s) 172
MluCI AATT 4 cut(s) 5, 76, 367, 478
MnlI CCTC 1 cut(s) 513
MseI TTAA 4 cut(s) 270, 335, 357, 381
MspI CCGG 2 cut(s) 180, 458
MvnI CGCG 1 cut(s) 364
MwoI GCNNNNNNNGC 1 cut(s) 164
NdeII GATC 1 cut(s) 172
NlaIII CATG 1 cut(s) 193
NmuCI GTSAC 2 cut(s) 130, 211
NspV TTCGAA 1 cut(s) 73
PfeI GAWTC 4 cut(s) 70, 432, 493, 499
PkrI GCNGC 1 cut(s) 400
Ppu21I YACGTR 1 cut(s) 296
PsuI RGATCY 1 cut(s) 172
RsaI GTAC 1 cut(s) 418
RsaNI GTAC 1 cut(s) 417
SaqAI TTAA 4 cut(s) 270, 335, 357, 381
SatI GCNGC 1 cut(s) 399
Sau3AI GATC 1 cut(s) 172
ScaI AGTACT 1 cut(s) 418
SfaNI GCATC 2 cut(s) 203, 310
SfcI CTRYAG 1 cut(s) 93
SfuI TTCGAA 1 cut(s) 73
Sse9I AATT 4 cut(s) 5, 76, 367, 478
SsiI CCGC 3 cut(s) 152, 165, 350
SspMI CTAG 1 cut(s) 506
TaaI ACNGT 4 cut(s) 47, 241, 379, 388
TaiI ACGT 3 cut(s) 29, 137, 298
TaqI TCGA 3 cut(s) 73, 141, 197
TasI AATT 4 cut(s) 5, 76, 367, 478
TatI WGTACW 1 cut(s) 416
TfiI GAWTC 4 cut(s) 70, 432, 493, 499
Tru1I TTAA 4 cut(s) 270, 335, 357, 381
Tru9I TTAA 4 cut(s) 270, 335, 357, 381
TseFI GTSAC 2 cut(s) 130, 211
TseI GCWGC 1 cut(s) 398
Tsp45I GTSAC 2 cut(s) 130, 211
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 72
XapI RAATTY 2 cut(s) 5, 76
XspI CTAG 1 cut(s) 506
ZrmI AGTACT 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.