MD06G1042400.v1.1

invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0002424
Physical Location & Seq
Reverse (-)
5570431 .. 5571003
573 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1042400.v1.1.491

Sequence Viewer

Length: 573 bp
ATGAGGAGTCTCTTCTCTTCGTTTTCCAAGATTTTCATCACCATCACCATCACTTTTCTGATGCTCTTTCACTGCACAATTGGTTCTAATAATCTCATCCAAAACTCTTGCAAGAAAGCCTCAGATGGTGATCCAAATTTGAGCTACAATTTCTGTGTTGCAAGCCTTGAGGAGGCCAACTCTAAATCCCACGGCCAAAGTCCAGATCACCTTGAACAACTAGTCCTCATTTCACTTAACCTTACCATATCCAACGCGACGAACATCAACTCCATCATTTCGAAGCTCTTGAAGGACAAACAGTTCGACAAGTACGCGAGGGATTGCTTGAAAGGGTGCTCAGAACTCTACTCGGACGCCATCCCTACGCTGCAAGAAGCTCTTTGCACCTTTCGGTCCAAGGATTTTCCCAAAGCAAATGTGGAAGTCAGCTCTGCCATGGACGCTTCTAGTACTTGTGAAGACGGTTTCAAGGATAAGAAAGGTGAAGTGTCGCCATTGAGAAAGGAGAATGATGTCTTCTTTCAGTTGAACGTCATTTCTCTTGCATTTATCAATACATTATCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

191

Amino Acids

21.06

Weight (kDa)

5.91

Isoelectric Point (pI)

37.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 31 - 183 3.2e-27 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 257, 317
AclWI GGATC 1 cut(s) 125
AcoI YGGCCR 1 cut(s) 193
AcsI RAATTY 1 cut(s) 136
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 2 cut(s) 314, 454
AfiI CCNNNNNNNGG 1 cut(s) 172
AgsI TTSAA 5 cut(s) 215, 292, 331, 472, 532
AhlI ACTAGT 1 cut(s) 220
AloI GAACNNNNNNTCC 6 cut(s) 207, 239, 254, 286, 287, 319
AluBI AGCT 4 cut(s) 144, 286, 380, 432
AluI AGCT 4 cut(s) 144, 286, 380, 432
Alw21I GWGCWC 1 cut(s) 341
Alw26I GTCTC 1 cut(s) 14
AlwI GGATC 1 cut(s) 125
AoxI GGCC 2 cut(s) 174, 193
ApeKI GCWGC 1 cut(s) 370
ApoI RAATTY 1 cut(s) 136
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
AspS9I GGNCC 1 cut(s) 396
AsuHPI GGTGA 5 cut(s) 31, 37, 140, 200, 497
AsuII TTCGAA 1 cut(s) 281
AvaII GGWCC 1 cut(s) 396
BbsI GAAGAC 2 cut(s) 468, 511
Bbv12I GWGCWC 1 cut(s) 341
BbvI GCAGC 1 cut(s) 357
BccI CCATC 5 cut(s) 50, 56, 119, 281, 368
BceAI ACGGC 1 cut(s) 208
BcoDI GTCTC 1 cut(s) 14
BcuI ACTAGT 1 cut(s) 220
BfaI CTAG 2 cut(s) 221, 450
BisI GCNGC 1 cut(s) 371
BlsI GCNGC 1 cut(s) 372
BmcAI AGTACT 1 cut(s) 454
Bme18I GGWCC 1 cut(s) 396
BmgT120I GGNCC 1 cut(s) 396
BmsI GCATC 1 cut(s) 51
BpiI GAAGAC 2 cut(s) 468, 511
BplI GAGNNNNNCTC 2 cut(s) 164, 196
Bpu14I TTCGAA 1 cut(s) 281
BpuEI CTTGAG 1 cut(s) 188
BsaBI GATNNNNATC 2 cut(s) 35, 129
BsaHI GRCGYC 1 cut(s) 357
BsaJI CCNNGG 3 cut(s) 190, 399, 438
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 1 cut(s) 172
Bse8I GATNNNNATC 2 cut(s) 35, 129
BseDI CCNNGG 3 cut(s) 190, 399, 438
BseGI GGATG 2 cut(s) 96, 360
BseJI GATNNNNATC 2 cut(s) 35, 129
BseLI CCNNNNNNNGG 1 cut(s) 172
BseMII CTCAG 2 cut(s) 135, 354
BseRI GAGGAG 2 cut(s) 19, 185
BseXI GCAGC 1 cut(s) 357
BsgI GTGCAG 1 cut(s) 58
Bsh1236I CGCG 2 cut(s) 257, 317
BshFI GGCC 2 cut(s) 176, 195
BsiHKAI GWGCWC 1 cut(s) 341
BslI CCNNNNNNNGG 1 cut(s) 172
BsmAI GTCTC 1 cut(s) 14
BsnI GGCC 2 cut(s) 176, 195
Bsp119I TTCGAA 1 cut(s) 281
Bsp1286I GDGCHC 1 cut(s) 341
Bsp143I GATC 2 cut(s) 130, 205
Bsp19I CCATGG 1 cut(s) 438
BspANI GGCC 2 cut(s) 176, 195
BspCNI CTCAG 2 cut(s) 134, 353
BspFNI CGCG 2 cut(s) 257, 317
BspPI GGATC 1 cut(s) 125
BspT104I TTCGAA 1 cut(s) 281
BssECI CCNNGG 3 cut(s) 190, 399, 438
BssMI GATC 2 cut(s) 130, 205
BssNI GRCGYC 1 cut(s) 357
BssT1I CCWWGG 2 cut(s) 399, 438
Bst4CI ACNGT 2 cut(s) 303, 467
Bst6I CTCTTC 2 cut(s) 17, 22
BstACI GRCGYC 1 cut(s) 357
BstBI TTCGAA 1 cut(s) 281
BstC8I GCNNGC 1 cut(s) 163
BstDEI CTNAG 2 cut(s) 121, 340
BstDSI CCRYGG 2 cut(s) 190, 438
BstENI CCTNNNNNAGG 1 cut(s) 170
BstF5I GGATG 2 cut(s) 96, 360
BstFNI CGCG 2 cut(s) 257, 317
BstKTI GATC 2 cut(s) 133, 208
BstMAI GTCTC 1 cut(s) 14
BstMBI GATC 2 cut(s) 130, 205
BstMWI GCNNNNNNNGC 1 cut(s) 443
BstUI CGCG 2 cut(s) 257, 317
BstV1I GCAGC 1 cut(s) 357
BstV2I GAAGAC 2 cut(s) 468, 511
BsuRI GGCC 2 cut(s) 176, 195
BtgI CCRYGG 2 cut(s) 190, 438
BtsCI GGATG 2 cut(s) 96, 360
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 1 cut(s) 70
Cac8I GCNNGC 1 cut(s) 163
Cfr13I GGNCC 1 cut(s) 396
CseI GACGC 2 cut(s) 365, 452
Csp6I GTAC 2 cut(s) 313, 453
CviAII CATG 1 cut(s) 439
CviJI RGCY 8 cut(s) 119, 144, 165, 176, 195, 286, 380, 432
CviKI_1 RGCY 8 cut(s) 119, 144, 165, 176, 195, 286, 380, 432
CviQI GTAC 2 cut(s) 313, 453
DdeI CTNAG 2 cut(s) 121, 340
DpnI GATC 2 cut(s) 132, 207
DpnII GATC 2 cut(s) 130, 205
EaeI YGGCCR 1 cut(s) 193
Eam1104I CTCTTC 2 cut(s) 17, 22
EarI CTCTTC 2 cut(s) 17, 22
Eco130I CCWWGG 2 cut(s) 399, 438
Eco47I GGWCC 1 cut(s) 396
EcoNI CCTNNNNNAGG 1 cut(s) 170
EcoT14I CCWWGG 2 cut(s) 399, 438
ErhI CCWWGG 2 cut(s) 399, 438
FaeI CATG 1 cut(s) 442
FaiI YATR 2 cut(s) 248, 440
FalI AAGNNNNNCTT 2 cut(s) 366, 398
FatI CATG 1 cut(s) 438
Fnu4HI GCNGC 1 cut(s) 371
FokI GGATG 2 cut(s) 83, 347
Fsp4HI GCNGC 1 cut(s) 371
FspBI CTAG 2 cut(s) 221, 450
GluI GCNGC 1 cut(s) 371
HaeIII GGCC 2 cut(s) 176, 195
HgaI GACGC 2 cut(s) 365, 452
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 1 cut(s) 442
HinfI GANTC 1 cut(s) 7
HphI GGTGA 5 cut(s) 31, 37, 140, 200, 497
Hpy188I TCNGA 4 cut(s) 60, 124, 343, 355
Hpy188III TCNNGA 2 cut(s) 203, 289
Hpy99I CGWCG 1 cut(s) 262
HpyAV CCTTC 1 cut(s) 286
HpyCH4III ACNGT 2 cut(s) 303, 467
HpyCH4IV ACGT 1 cut(s) 534
HpyCH4V TGCA 6 cut(s) 75, 111, 161, 373, 387, 548
HpyF10VI GCNNNNNNNGC 1 cut(s) 443
HpyF3I CTNAG 2 cut(s) 121, 340
HpySE526I ACGT 1 cut(s) 534
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 1 cut(s) 442
Kzo9I GATC 2 cut(s) 130, 205
LpnPI CCDG 1 cut(s) 216
Lsp1109I GCAGC 1 cut(s) 357
LweI GCATC 1 cut(s) 51
MaeI CTAG 2 cut(s) 221, 450
MaeII ACGT 1 cut(s) 534
MalI GATC 2 cut(s) 132, 207
MboI GATC 2 cut(s) 130, 205
MboII GAAGA 4 cut(s) 4, 9, 473, 511
MfeI CAATTG 1 cut(s) 78
MhlI GDGCHC 1 cut(s) 341
MluCI AATT 4 cut(s) 78, 136, 148, 568
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 1 cut(s) 276
MnlI CCTC 5 cut(s) 130, 163, 166, 236, 312
MseI TTAA 1 cut(s) 237
MunI CAATTG 1 cut(s) 78
MvnI CGCG 2 cut(s) 257, 317
MwoI GCNNNNNNNGC 1 cut(s) 443
NcoI CCATGG 1 cut(s) 438
NdeII GATC 2 cut(s) 130, 205
NlaIII CATG 1 cut(s) 442
NspV TTCGAA 1 cut(s) 281
PkrI GCNGC 1 cut(s) 372
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
PspPI GGNCC 1 cut(s) 396
RsaI GTAC 2 cut(s) 314, 454
RsaNI GTAC 2 cut(s) 313, 453
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 1 cut(s) 371
Sau3AI GATC 2 cut(s) 130, 205
Sau96I GGNCC 1 cut(s) 396
ScaI AGTACT 1 cut(s) 454
SchI GAGTC 1 cut(s) 16
SduI GDGCHC 1 cut(s) 341
SetI ASST 9 cut(s) 146, 213, 243, 288, 382, 392, 434, 487, 537
SfaNI GCATC 1 cut(s) 51
SfuI TTCGAA 1 cut(s) 281
SinI GGWCC 1 cut(s) 396
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
SpeI ACTAGT 1 cut(s) 220
Sse9I AATT 4 cut(s) 78, 136, 148, 568
SspMI CTAG 2 cut(s) 221, 450
StyI CCWWGG 2 cut(s) 399, 438
TaaI ACNGT 2 cut(s) 303, 467
TaiI ACGT 1 cut(s) 537
TaqI TCGA 2 cut(s) 281, 306
TaqII GACCGA 1 cut(s) 384
TasI AATT 4 cut(s) 78, 136, 148, 568
TatI WGTACW 1 cut(s) 452
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 1 cut(s) 77
TseI GCWGC 1 cut(s) 370
TspDTI ATGAA 1 cut(s) 25
TspRI CASTG 1 cut(s) 77
VpaK11BI GGWCC 1 cut(s) 396
XagI CCTNNNNNAGG 1 cut(s) 170
XapI RAATTY 1 cut(s) 136
XcmI CCANNNNNNNNNTGG 1 cut(s) 418
XspI CTAG 2 cut(s) 221, 450
ZrmI AGTACT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.