Rh7DG246700

Invertase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0002424
Physical Location & Seq
Reverse (-)
25438461 .. 25439054
594 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG246700.1

Sequence Viewer

Length: 594 bp
ATGACCCCAGTGACGACGTCAAAAAAGATTCCATTCAAGGAGGAAGGTATTTGCCAGAATTCCATACCCTATTGTAGTGAACCTCCAACCCTAACAGAGCCGCTCCACTATATGCTTCTCAAAATGAGGTGTTCCTTCCTTGTCATTTTTCTCATGCTCTTTCACAACACAATCGATGATGATTTGATCCAACAATATTGCAAGAAAGCCGCAGATCACGAACCATCTGTGAACTACGACTTTTGTGTCTCAACCATTGAAGCCCACCCTAAAAGCCATAGTGCACACCTTGCAACACTTATAATGACTGTACTTGACCTAGCCGGAGCCAAGGTGACCAACTTCAATTCACACATTCAAGAGCTAAAGAAGGATCCAAAAGTTGACCAACCTACATTACAAGACTACGAAGAAGTATACTTAGATGCCACTAGTAGCATGCAGGAAGCTACCAATTCCTTCAAGGCCAAGAATTATGAGTCGACTCTTAATACTCTTAGTGCTGCTATGAATGCACCTGATACTTGTGAAACTGGATTTCAAGAGAGGAAAGCAGTGTCTCCCTTGAAGAAGGAGGACGATGACTTCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

197

Amino Acids

22.22

Weight (kDa)

5.09

Isoelectric Point (pI)

31.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 61 - 191 5.2e-21 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 302
AasI GACNNNNNNGTC 1 cut(s) 245
AatII GACGTC 1 cut(s) 20
AccBSI CCGCTC 1 cut(s) 103
AccI GTMKAC 2 cut(s) 417, 482
AciI CCGC 2 cut(s) 101, 210
AclWI GGATC 3 cut(s) 181, 368, 381
AcsI RAATTY 1 cut(s) 58
AcyI GRCGYC 1 cut(s) 17
AfaI GTAC 1 cut(s) 312
AgsI TTSAA 7 cut(s) 37, 260, 346, 359, 463, 542, 568
AhlI ACTAGT 1 cut(s) 431
AluBI AGCT 2 cut(s) 364, 449
AluI AGCT 2 cut(s) 364, 449
Alw21I GWGCWC 1 cut(s) 286
Alw26I GTCTC 2 cut(s) 253, 564
Alw44I GTGCAC 1 cut(s) 282
AlwI GGATC 3 cut(s) 181, 368, 381
AoxI GGCC 1 cut(s) 465
ApaLI GTGCAC 1 cut(s) 282
ApeKI GCWGC 1 cut(s) 503
ApoI RAATTY 1 cut(s) 58
AsuHPI GGTGA 1 cut(s) 346
BaeGI GKGCMC 1 cut(s) 286
BamHI GGATCC 1 cut(s) 373
Bbv12I GWGCWC 1 cut(s) 286
BbvI GCAGC 1 cut(s) 490
BccI CCATC 1 cut(s) 232
BcoDI GTCTC 2 cut(s) 253, 564
BcuI ACTAGT 1 cut(s) 431
BfaI CTAG 2 cut(s) 320, 432
BisI GCNGC 3 cut(s) 101, 210, 504
BlsI GCNGC 3 cut(s) 102, 211, 505
BmiI GGNNCC 2 cut(s) 328, 375
BmsI GCATC 1 cut(s) 415
Bsa29I ATCGAT 1 cut(s) 174
BsaHI GRCGYC 1 cut(s) 17
BsaJI CCNNGG 1 cut(s) 330
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bse1I ACTGG 2 cut(s) 8, 538
BseCI ATCGAT 1 cut(s) 174
BseDI CCNNGG 1 cut(s) 330
BseNI ACTGG 2 cut(s) 8, 538
BseSI GKGCMC 1 cut(s) 286
BseXI GCAGC 1 cut(s) 490
BshFI GGCC 1 cut(s) 467
BshVI ATCGAT 1 cut(s) 174
BsiHKAI GWGCWC 1 cut(s) 286
BsiSI CCGG 1 cut(s) 324
BsmAI GTCTC 2 cut(s) 253, 564
BsmI GAATGC 1 cut(s) 517
BsnI GGCC 1 cut(s) 467
Bsp1286I GDGCHC 1 cut(s) 286
Bsp143I GATC 3 cut(s) 186, 214, 373
BspACI CCGC 2 cut(s) 101, 210
BspANI GGCC 1 cut(s) 467
BspDI ATCGAT 1 cut(s) 174
BspLI GGNNCC 2 cut(s) 328, 375
BspPI GGATC 3 cut(s) 181, 368, 381
BsrBI CCGCTC 1 cut(s) 103
BsrI ACTGG 2 cut(s) 8, 538
BssECI CCNNGG 1 cut(s) 330
BssMI GATC 3 cut(s) 186, 214, 373
BssNAI GTATAC 1 cut(s) 418
BssNI GRCGYC 1 cut(s) 17
BssT1I CCWWGG 1 cut(s) 330
Bst1107I GTATAC 1 cut(s) 418
Bst4CI ACNGT 1 cut(s) 310
BstACI GRCGYC 1 cut(s) 17
BstAPI GCANNNNNTGC 1 cut(s) 290
BstC8I GCNNGC 1 cut(s) 440
BstDEI CTNAG 2 cut(s) 421, 497
BstEII GGTNACC 1 cut(s) 334
BstKTI GATC 3 cut(s) 189, 217, 376
BstMAI GTCTC 2 cut(s) 253, 564
BstMBI GATC 3 cut(s) 186, 214, 373
BstMWI GCNNNNNNNGC 2 cut(s) 290, 512
BstNSI RCATGY 1 cut(s) 442
BstPI GGTNACC 1 cut(s) 334
BstSLI GKGCMC 1 cut(s) 286
BstV1I GCAGC 1 cut(s) 490
BstX2I RGATCY 1 cut(s) 373
BstYI RGATCY 1 cut(s) 373
BstZ17I GTATAC 1 cut(s) 418
Bsu15I ATCGAT 1 cut(s) 174
BsuRI GGCC 1 cut(s) 467
BsuTUI ATCGAT 1 cut(s) 174
BtsI GCAGTG 1 cut(s) 561
BtsIMutI CAGTG 2 cut(s) 15, 561
Cac8I GCNNGC 1 cut(s) 440
ClaI ATCGAT 1 cut(s) 174
Csp6I GTAC 1 cut(s) 311
CviAII CATG 2 cut(s) 154, 439
CviJI RGCY 9 cut(s) 100, 209, 263, 276, 323, 329, 364, 449, 467
CviKI_1 RGCY 9 cut(s) 100, 209, 263, 276, 323, 329, 364, 449, 467
CviQI GTAC 1 cut(s) 311
DdeI CTNAG 2 cut(s) 421, 497
DpnI GATC 3 cut(s) 188, 216, 375
DpnII GATC 3 cut(s) 186, 214, 373
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
Eco130I CCWWGG 1 cut(s) 330
Eco91I GGTNACC 1 cut(s) 334
EcoO65I GGTNACC 1 cut(s) 334
EcoRI GAATTC 1 cut(s) 58
EcoT14I CCWWGG 1 cut(s) 330
ErhI CCWWGG 1 cut(s) 330
FaeI CATG 2 cut(s) 157, 442
FatI CATG 2 cut(s) 153, 438
FblI GTMKAC 2 cut(s) 417, 482
Fnu4HI GCNGC 3 cut(s) 101, 210, 504
Fsp4HI GCNGC 3 cut(s) 101, 210, 504
FspBI CTAG 2 cut(s) 320, 432
GluI GCNGC 3 cut(s) 101, 210, 504
HaeIII GGCC 1 cut(s) 467
HapII CCGG 1 cut(s) 324
Hin1I GRCGYC 1 cut(s) 17
Hin1II CATG 2 cut(s) 157, 442
HincII GTYRAC 2 cut(s) 385, 483
HindII GTYRAC 2 cut(s) 385, 483
HinfI GANTC 3 cut(s) 28, 479, 484
HpaII CCGG 1 cut(s) 324
HphI GGTGA 1 cut(s) 346
Hpy166II GTNNAC 6 cut(s) 80, 232, 284, 385, 418, 483
Hpy188III TCNNGA 3 cut(s) 218, 359, 542
Hpy8I GTNNAC 6 cut(s) 80, 232, 284, 385, 418, 483
Hpy99I CGWCG 1 cut(s) 19
HpyAV CCTTC 5 cut(s) 38, 145, 364, 469, 565
HpyCH4III ACNGT 1 cut(s) 310
HpyCH4IV ACGT 1 cut(s) 17
HpyCH4V TGCA 5 cut(s) 201, 284, 293, 442, 515
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 512
HpyF3I CTNAG 2 cut(s) 421, 497
HpySE526I ACGT 1 cut(s) 17
Hsp92I GRCGYC 1 cut(s) 17
Hsp92II CATG 2 cut(s) 157, 442
Kzo9I GATC 3 cut(s) 186, 214, 373
LmnI GCTCC 2 cut(s) 108, 326
LpnPI CCDG 6 cut(s) 21, 68, 337, 428, 519, 531
Lsp1109I GCAGC 1 cut(s) 490
LweI GCATC 1 cut(s) 415
MaeI CTAG 2 cut(s) 320, 432
MaeII ACGT 1 cut(s) 17
MaeIII GTNAC 2 cut(s) 10, 334
MalI GATC 3 cut(s) 188, 216, 375
MbiI CCGCTC 1 cut(s) 103
MboI GATC 3 cut(s) 186, 214, 373
MboII GAAGA 2 cut(s) 422, 580
MflI RGATCY 1 cut(s) 373
MhlI GDGCHC 1 cut(s) 286
MluCI AATT 4 cut(s) 58, 346, 454, 472
MlyI GAGTC 2 cut(s) 478, 488
MmeI TCCRAC 2 cut(s) 110, 214
MnlI CCTC 5 cut(s) 34, 93, 120, 540, 568
MseI TTAA 2 cut(s) 489, 592
MspI CCGG 1 cut(s) 324
Mva1269I GAATGC 1 cut(s) 517
MwoI GCNNNNNNNGC 2 cut(s) 290, 512
NdeII GATC 3 cut(s) 186, 214, 373
NlaIII CATG 2 cut(s) 157, 442
NlaIV GGNNCC 2 cut(s) 328, 375
NmuCI GTSAC 2 cut(s) 10, 334
NspI RCATGY 1 cut(s) 442
PaeI GCATGC 1 cut(s) 442
PctI GAATGC 1 cut(s) 517
PfeI GAWTC 1 cut(s) 28
PflFI GACNNNGTC 1 cut(s) 16
PkrI GCNGC 3 cut(s) 102, 211, 505
PleI GAGTC 2 cut(s) 478, 487
PpsI GAGTC 2 cut(s) 478, 487
PsiI TTATAA 1 cut(s) 302
PspEI GGTNACC 1 cut(s) 334
PspN4I GGNNCC 2 cut(s) 328, 375
PsuI RGATCY 1 cut(s) 373
PsyI GACNNNGTC 1 cut(s) 16
RsaI GTAC 1 cut(s) 312
RsaNI GTAC 1 cut(s) 311
SalI GTCGAC 1 cut(s) 481
SaqAI TTAA 2 cut(s) 489, 592
SatI GCNGC 3 cut(s) 101, 210, 504
Sau3AI GATC 3 cut(s) 186, 214, 373
SchI GAGTC 2 cut(s) 478, 488
SduI GDGCHC 1 cut(s) 286
SfaNI GCATC 1 cut(s) 415
SpeI ACTAGT 1 cut(s) 431
SphI GCATGC 1 cut(s) 442
Sse9I AATT 4 cut(s) 58, 346, 454, 472
SsiI CCGC 2 cut(s) 101, 210
SspI AATATT 1 cut(s) 197
SspMI CTAG 2 cut(s) 320, 432
StyI CCWWGG 1 cut(s) 330
TaaI ACNGT 1 cut(s) 310
TaiI ACGT 1 cut(s) 20
TaqI TCGA 2 cut(s) 174, 482
TasI AATT 4 cut(s) 58, 346, 454, 472
TatI WGTACW 1 cut(s) 310
TauI GCSGC 2 cut(s) 103, 212
TfiI GAWTC 1 cut(s) 28
Tru1I TTAA 2 cut(s) 489, 592
Tru9I TTAA 2 cut(s) 489, 592
TscAI CASTG 2 cut(s) 15, 561
TseFI GTSAC 2 cut(s) 10, 334
TseI GCWGC 1 cut(s) 503
Tsp45I GTSAC 2 cut(s) 10, 334
TspDTI ATGAA 1 cut(s) 524
TspRI CASTG 2 cut(s) 15, 561
Tth111I GACNNNGTC 1 cut(s) 16
VneI GTGCAC 1 cut(s) 282
XapI RAATTY 1 cut(s) 58
XceI RCATGY 1 cut(s) 442
XmiI GTMKAC 2 cut(s) 417, 482
XspI CTAG 2 cut(s) 320, 432
ZraI GACGTC 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.