AT5G46990

invertase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002424
Physical Location & Seq
Reverse (-)
19067455 .. 19068292
838 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46990.1

Sequence Viewer

Length: 564 bp
ATGAAGCTCTTGTTTTACCTTGTTGTGTTCTTTCTCCTTTTAAATGGTTTCACAGCCAACAGAGTTGCAAATTCCCTGATTCGAGATTCTTGCAAGAAAGCTTCAAAAATGTCGGAACCACATTACTACAAGTTCTGCATTGCATCTATCAGCGAAAACCCGGAGAGTCAAAAAGTAAGAAATATCGATGAATTGATCGGAGTAGGAGTGAAAAACGCTATTTCGAACATGACGAACGTGAAAGGAATTGTGGAGAGGATTCTGAAGGACAGAAAGTATACGAGTAAAATGAGTGAGAAGATGTTGCGCGTTTGCGTTAAGCTTTATTCTGAGGGCACTAACTTGTTAACCAAATGTTTAGAGTACATTAAATTGCGGGATTTCGATAAAGTCCACAATACTATTCGTCATGCAAGAGTTGTTCCGAGAGAGTGTGAAATGGGATTCAATGACGATAATAAACAGAAATCTCCGGTGACGAAAGAGAACGATGTTCTATTCGACACCGTCGACATTGCTCAGTCTTTTAATTACTATGCTCATATAAGGCCAGACATCGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

187

Amino Acids

21.62

Weight (kDa)

9.22

Isoelectric Point (pI)

48.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 25 - 173 1.6e-18 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 278, 510
AccII CGCG 1 cut(s) 309
AciI CCGC 1 cut(s) 376
AcsI RAATTY 1 cut(s) 70
AcuI CTGAAG 1 cut(s) 284
AfaI GTAC 1 cut(s) 365
AgsI TTSAA 2 cut(s) 105, 448
AluBI AGCT 3 cut(s) 7, 101, 322
AluI AGCT 3 cut(s) 7, 101, 322
AoxI GGCC 1 cut(s) 548
ApoI RAATTY 1 cut(s) 70
AspLEI GCGC 1 cut(s) 309
AsuC2I CCSGG 1 cut(s) 161
AsuHPI GGTGA 1 cut(s) 487
AsuII TTCGAA 1 cut(s) 224
BaeGI GKGCMC 1 cut(s) 338
BcgI CGANNNNNNTGC 2 cut(s) 72, 106
BcnI CCSGG 1 cut(s) 161
Bme1390I CCNGG 1 cut(s) 161
BmiI GGNNCC 1 cut(s) 117
BmrFI CCNGG 1 cut(s) 161
BmsI GCATC 1 cut(s) 152
Bpu14I TTCGAA 1 cut(s) 224
BpuMI CCSGG 1 cut(s) 161
Bsa29I ATCGAT 1 cut(s) 186
BsaWI WCCGGW 1 cut(s) 472
BsaXI ACNNNNNCTCC 2 cut(s) 192, 222
Bse3DI GCAATG 2 cut(s) 138, 513
BseCI ATCGAT 1 cut(s) 186
BseMI GCAATG 2 cut(s) 138, 513
BseMII CTCAG 2 cut(s) 321, 533
BseSI GKGCMC 1 cut(s) 338
Bsh1236I CGCG 1 cut(s) 309
BshFI GGCC 1 cut(s) 550
BshVI ATCGAT 1 cut(s) 186
BsiSI CCGG 2 cut(s) 161, 473
BsnI GGCC 1 cut(s) 550
Bsp119I TTCGAA 1 cut(s) 224
Bsp1286I GDGCHC 1 cut(s) 338
Bsp143I GATC 1 cut(s) 195
BspACI CCGC 1 cut(s) 376
BspANI GGCC 1 cut(s) 550
BspCNI CTCAG 2 cut(s) 322, 532
BspDI ATCGAT 1 cut(s) 186
BspFNI CGCG 1 cut(s) 309
BspLI GGNNCC 1 cut(s) 117
BspT104I TTCGAA 1 cut(s) 224
BsrDI GCAATG 2 cut(s) 138, 513
BssMI GATC 1 cut(s) 195
BssNAI GTATAC 1 cut(s) 279
Bst1107I GTATAC 1 cut(s) 279
Bst4CI ACNGT 1 cut(s) 508
BstBI TTCGAA 1 cut(s) 224
BstDEI CTNAG 2 cut(s) 330, 519
BstFNI CGCG 1 cut(s) 309
BstHHI GCGC 1 cut(s) 309
BstKTI GATC 1 cut(s) 198
BstMBI GATC 1 cut(s) 195
BstSCI CCNGG 1 cut(s) 159
BstSLI GKGCMC 1 cut(s) 338
BstUI CGCG 1 cut(s) 309
BstZ17I GTATAC 1 cut(s) 279
Bsu15I ATCGAT 1 cut(s) 186
BsuRI GGCC 1 cut(s) 550
BsuTUI ATCGAT 1 cut(s) 186
CfoI GCGC 1 cut(s) 309
ClaI ATCGAT 1 cut(s) 186
Csp6I GTAC 1 cut(s) 364
CviAII CATG 2 cut(s) 229, 410
CviJI RGCY 5 cut(s) 7, 56, 101, 322, 550
CviKI_1 RGCY 5 cut(s) 7, 56, 101, 322, 550
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 2 cut(s) 330, 519
DpnI GATC 1 cut(s) 197
DpnII GATC 1 cut(s) 195
DraI TTTAAA 1 cut(s) 42
Eco57I CTGAAG 1 cut(s) 284
FaeI CATG 2 cut(s) 232, 413
FaiI YATR 7 cut(s) 230, 279, 411, 537, 543, 545, 562
FatI CATG 2 cut(s) 228, 409
FauI CCCGC 1 cut(s) 369
FblI GTMKAC 2 cut(s) 278, 510
GlaI GCGC 1 cut(s) 308
HaeIII GGCC 1 cut(s) 550
HapII CCGG 2 cut(s) 161, 473
HhaI GCGC 1 cut(s) 309
Hin1II CATG 2 cut(s) 232, 413
Hin6I GCGC 1 cut(s) 307
HinP1I GCGC 1 cut(s) 307
HincII GTYRAC 2 cut(s) 348, 511
HindII GTYRAC 2 cut(s) 348, 511
HindIII AAGCTT 2 cut(s) 99, 320
HinfI GANTC 5 cut(s) 79, 86, 166, 259, 444
HpaI GTTAAC 1 cut(s) 348
HpaII CCGG 2 cut(s) 161, 473
HphI GGTGA 1 cut(s) 487
Hpy166II GTNNAC 4 cut(s) 279, 348, 394, 511
Hpy188I TCNGA 5 cut(s) 115, 200, 264, 331, 426
Hpy188III TCNNGA 1 cut(s) 83
Hpy8I GTNNAC 4 cut(s) 279, 348, 394, 511
Hpy99I CGWCG 1 cut(s) 512
HpyAV CCTTC 1 cut(s) 259
HpyCH4III ACNGT 1 cut(s) 508
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 5 cut(s) 68, 93, 138, 143, 413
HpyF3I CTNAG 2 cut(s) 330, 519
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 2 cut(s) 232, 413
HspAI GCGC 1 cut(s) 307
KspAI GTTAAC 1 cut(s) 348
Kzo9I GATC 1 cut(s) 195
LpnPI CCDG 3 cut(s) 89, 174, 486
LweI GCATC 1 cut(s) 152
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 1 cut(s) 475
MalI GATC 1 cut(s) 197
MboI GATC 1 cut(s) 195
MboII GAAGA 1 cut(s) 310
MhlI GDGCHC 1 cut(s) 338
MluCI AATT 5 cut(s) 70, 191, 246, 371, 529
MlyI GAGTC 1 cut(s) 175
MmeI TCCRAC 1 cut(s) 93
MnlI CCTC 2 cut(s) 249, 325
MseI TTAA 5 cut(s) 41, 318, 347, 369, 528
MspI CCGG 2 cut(s) 161, 473
MspR9I CCNGG 1 cut(s) 161
MvnI CGCG 1 cut(s) 309
NciI CCSGG 1 cut(s) 161
NdeII GATC 1 cut(s) 195
NlaIII CATG 2 cut(s) 232, 413
NlaIV GGNNCC 1 cut(s) 117
NmuCI GTSAC 1 cut(s) 475
NspV TTCGAA 1 cut(s) 224
PcsI WCGNNNNNNNCGW 2 cut(s) 230, 507
PfeI GAWTC 4 cut(s) 79, 86, 259, 444
PflFI GACNNNGTC 1 cut(s) 506
PleI GAGTC 1 cut(s) 174
PpsI GAGTC 1 cut(s) 174
PspN4I GGNNCC 1 cut(s) 117
PsyI GACNNNGTC 1 cut(s) 506
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
SalI GTCGAC 1 cut(s) 509
SaqAI TTAA 5 cut(s) 41, 318, 347, 369, 528
Sau3AI GATC 1 cut(s) 195
SchI GAGTC 1 cut(s) 175
ScrFI CCNGG 1 cut(s) 161
SduI GDGCHC 1 cut(s) 338
SetI ASST 5 cut(s) 9, 21, 103, 240, 324
SfaNI GCATC 1 cut(s) 152
SfuI TTCGAA 1 cut(s) 224
Sse9I AATT 5 cut(s) 70, 191, 246, 371, 529
SsiI CCGC 1 cut(s) 376
StyD4I CCNGG 1 cut(s) 159
TaaI ACNGT 1 cut(s) 508
TaiI ACGT 1 cut(s) 240
TaqI TCGA 6 cut(s) 82, 186, 224, 384, 501, 510
TasI AATT 5 cut(s) 70, 191, 246, 371, 529
TatI WGTACW 1 cut(s) 363
TfiI GAWTC 4 cut(s) 79, 86, 259, 444
Tru1I TTAA 5 cut(s) 41, 318, 347, 369, 528
Tru9I TTAA 5 cut(s) 41, 318, 347, 369, 528
TseFI GTSAC 1 cut(s) 475
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 2 cut(s) 17, 204
Tth111I GACNNNGTC 1 cut(s) 506
XapI RAATTY 1 cut(s) 70
XmiI GTMKAC 2 cut(s) 278, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.