AT5G46960

invertase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002424
Physical Location & Seq
Reverse (-)
19062146 .. 19062814
669 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46960.1

Sequence Viewer

Length: 525 bp
ATGAAGTTCTTGGTTTCATTGGTTATCTTCTCTCTTTTCTTAAACGGTTTCGCAACCGCTCAAACTCTCATTCAAGATTCTTGCAAGAAAGCTTTTGCGAAAGACCCGCAATTGTCATACGATTTCTGCGTCAATTCTCTTACACAAGATCCACAAAGCAAAGCCGCGACTACTCTCGAAAGTTTGGTCCTAGCATCGACGAAGACCGCTGCGGCAAAAATCACGAACTTGAAAGGAATCGTTGCACAGGATCTCAAAGACCAGAGATATCAGGATATTGTGGAAGACTTAAAACTTTGCCTCGGATTTTATAACGATGCTAATGATGATTTAACAACTGCTTTAGCGAACATTAAATCGCGTGATTATCAAGGCGCTAACATTAATCTGAGTGCTGCTTTGGATGTACCAGGCAATTGCGAGGATGATTTCAAGGAAGCAAAAAAGACGTCTCCGATTACCAACGAGAACAGTATTTTGTTTAAGACGATTTTGATTCCTTTGGCTTTTACTAATATGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

174

Amino Acids

19.07

Weight (kDa)

4.93

Isoelectric Point (pI)

28.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 23 - 169 2.5e-21 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 312
AatII GACGTC 1 cut(s) 452
AccBSI CCGCTC 1 cut(s) 59
AccII CGCG 2 cut(s) 167, 361
AciI CCGC 5 cut(s) 57, 107, 165, 207, 212
AclWI GGATC 2 cut(s) 143, 258
AcyI GRCGYC 1 cut(s) 449
AfaI GTAC 1 cut(s) 408
AgsI TTSAA 3 cut(s) 74, 232, 433
AjnI CCWGG 1 cut(s) 409
AluBI AGCT 1 cut(s) 92
AluI AGCT 1 cut(s) 92
Alw26I GTCTC 1 cut(s) 456
AlwI GGATC 2 cut(s) 143, 258
ApeKI GCWGC 2 cut(s) 209, 395
ArsI GACNNNNNNTTYG 4 cut(s) 171, 203, 434, 466
AseI ATTAAT 1 cut(s) 384
AspLEI GCGC 1 cut(s) 377
AspS9I GGNCC 1 cut(s) 187
AvaII GGWCC 1 cut(s) 187
BbsI GAAGAC 2 cut(s) 209, 291
BbvI GCAGC 2 cut(s) 196, 382
BciT130I CCWGG 1 cut(s) 411
BcoDI GTCTC 1 cut(s) 456
BfaI CTAG 1 cut(s) 191
BfoI RGCGCY 1 cut(s) 378
BisI GCNGC 4 cut(s) 165, 210, 213, 396
BlsI GCNGC 4 cut(s) 166, 211, 214, 397
Bme1390I CCNGG 1 cut(s) 411
Bme18I GGWCC 1 cut(s) 187
BmgT120I GGNCC 1 cut(s) 187
BmrFI CCNGG 1 cut(s) 411
BmsI GCATC 2 cut(s) 203, 307
BpiI GAAGAC 2 cut(s) 209, 291
BsaHI GRCGYC 1 cut(s) 449
BsaJI CCNNGG 1 cut(s) 301
BseBI CCWGG 1 cut(s) 411
BseDI CCNNGG 1 cut(s) 301
BseGI GGATG 2 cut(s) 409, 430
BseMII CTCAG 1 cut(s) 380
BseXI GCAGC 2 cut(s) 196, 382
Bsh1236I CGCG 2 cut(s) 167, 361
BsmAI GTCTC 1 cut(s) 456
BsmBI CGTCTC 1 cut(s) 456
Bsp143I GATC 2 cut(s) 148, 250
BspACI CCGC 5 cut(s) 57, 107, 165, 207, 212
BspCNI CTCAG 1 cut(s) 381
BspFNI CGCG 2 cut(s) 167, 361
BspPI GGATC 2 cut(s) 143, 258
BsrBI CCGCTC 1 cut(s) 59
BssECI CCNNGG 1 cut(s) 301
BssMI GATC 2 cut(s) 148, 250
BssNI GRCGYC 1 cut(s) 449
Bst2UI CCWGG 1 cut(s) 411
Bst4CI ACNGT 2 cut(s) 47, 473
BstACI GRCGYC 1 cut(s) 449
BstDEI CTNAG 1 cut(s) 389
BstF5I GGATG 2 cut(s) 409, 430
BstFNI CGCG 2 cut(s) 167, 361
BstH2I RGCGCY 1 cut(s) 378
BstHHI GCGC 1 cut(s) 377
BstKTI GATC 2 cut(s) 151, 253
BstMAI GTCTC 1 cut(s) 456
BstMBI GATC 2 cut(s) 148, 250
BstNI CCWGG 1 cut(s) 411
BstSCI CCNGG 1 cut(s) 409
BstUI CGCG 2 cut(s) 167, 361
BstV1I GCAGC 2 cut(s) 196, 382
BstV2I GAAGAC 2 cut(s) 209, 291
BstX2I RGATCY 2 cut(s) 148, 250
BstYI RGATCY 2 cut(s) 148, 250
BtsCI GGATG 2 cut(s) 409, 430
CfoI GCGC 1 cut(s) 377
Cfr13I GGNCC 1 cut(s) 187
CseI GACGC 1 cut(s) 118
Csp6I GTAC 1 cut(s) 407
CviJI RGCY 3 cut(s) 92, 164, 506
CviKI_1 RGCY 3 cut(s) 92, 164, 506
CviQI GTAC 1 cut(s) 407
DdeI CTNAG 1 cut(s) 389
DpnI GATC 2 cut(s) 150, 252
DpnII GATC 2 cut(s) 148, 250
Eco32I GATATC 1 cut(s) 269
Eco47I GGWCC 1 cut(s) 187
EcoRII CCWGG 1 cut(s) 409
EcoRV GATATC 1 cut(s) 269
Esp3I CGTCTC 1 cut(s) 456
FaiI YATR 3 cut(s) 118, 312, 518
FauI CCCGC 1 cut(s) 114
Fnu4HI GCNGC 4 cut(s) 165, 210, 213, 396
FokI GGATG 2 cut(s) 416, 437
Fsp4HI GCNGC 4 cut(s) 165, 210, 213, 396
FspBI CTAG 1 cut(s) 191
GlaI GCGC 1 cut(s) 376
GluI GCNGC 4 cut(s) 165, 210, 213, 396
HaeII RGCGCY 1 cut(s) 378
HgaI GACGC 1 cut(s) 118
HhaI GCGC 1 cut(s) 377
Hin1I GRCGYC 1 cut(s) 449
Hin6I GCGC 1 cut(s) 375
HinP1I GCGC 1 cut(s) 375
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 3 cut(s) 77, 237, 496
Hpy188I TCNGA 3 cut(s) 305, 390, 456
Hpy188III TCNNGA 4 cut(s) 74, 176, 223, 272
Hpy99I CGWCG 1 cut(s) 202
HpyCH4III ACNGT 2 cut(s) 47, 473
HpyCH4IV ACGT 1 cut(s) 449
HpyCH4V TGCA 2 cut(s) 84, 245
HpyF3I CTNAG 1 cut(s) 389
HpySE526I ACGT 1 cut(s) 449
Hsp92I GRCGYC 1 cut(s) 449
HspAI GCGC 1 cut(s) 375
Kzo9I GATC 2 cut(s) 148, 250
LpnPI CCDG 5 cut(s) 233, 257, 275, 396, 423
Lsp1109I GCAGC 2 cut(s) 196, 382
LweI GCATC 2 cut(s) 203, 307
MaeI CTAG 1 cut(s) 191
MaeII ACGT 1 cut(s) 449
MalI GATC 2 cut(s) 150, 252
MbiI CCGCTC 1 cut(s) 59
MboI GATC 2 cut(s) 148, 250
MboII GAAGA 3 cut(s) 19, 214, 296
MfeI CAATTG 2 cut(s) 110, 415
MflI RGATCY 2 cut(s) 148, 250
MluCI AATT 3 cut(s) 110, 133, 415
MnlI CCTC 2 cut(s) 311, 415
MseI TTAA 6 cut(s) 41, 290, 332, 354, 384, 483
MspA1I CMGCKG 1 cut(s) 209
MspR9I CCNGG 1 cut(s) 411
MunI CAATTG 2 cut(s) 110, 415
MvaI CCWGG 1 cut(s) 411
MvnI CGCG 2 cut(s) 167, 361
NdeII GATC 2 cut(s) 148, 250
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PfeI GAWTC 3 cut(s) 77, 237, 496
PkrI GCNGC 4 cut(s) 166, 211, 214, 397
PshBI ATTAAT 1 cut(s) 384
PsiI TTATAA 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 409
PspGI CCWGG 1 cut(s) 409
PspPI GGNCC 1 cut(s) 187
PsuI RGATCY 2 cut(s) 148, 250
RsaI GTAC 1 cut(s) 408
RsaNI GTAC 1 cut(s) 407
SaqAI TTAA 6 cut(s) 41, 290, 332, 354, 384, 483
SatI GCNGC 4 cut(s) 165, 210, 213, 396
Sau3AI GATC 2 cut(s) 148, 250
Sau96I GGNCC 1 cut(s) 187
ScrFI CCNGG 1 cut(s) 411
SetI ASST 2 cut(s) 94, 452
SfaNI GCATC 2 cut(s) 203, 307
SinI GGWCC 1 cut(s) 187
Sse9I AATT 3 cut(s) 110, 133, 415
SsiI CCGC 5 cut(s) 57, 107, 165, 207, 212
SspMI CTAG 1 cut(s) 191
StyD4I CCNGG 1 cut(s) 409
TaaI ACNGT 2 cut(s) 47, 473
TaiI ACGT 1 cut(s) 452
TaqI TCGA 2 cut(s) 177, 197
TasI AATT 3 cut(s) 110, 133, 415
TauI GCSGC 2 cut(s) 167, 215
TfiI GAWTC 3 cut(s) 77, 237, 496
Tru1I TTAA 6 cut(s) 41, 290, 332, 354, 384, 483
Tru9I TTAA 6 cut(s) 41, 290, 332, 354, 384, 483
TseI GCWGC 2 cut(s) 209, 395
TspDTI ATGAA 2 cut(s) 6, 17
VpaK11BI GGWCC 1 cut(s) 187
VspI ATTAAT 1 cut(s) 384
XspI CTAG 1 cut(s) 191
ZraI GACGTC 1 cut(s) 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.