FvH4_5g21030

Invertase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002424
Physical Location & Seq
Forward (+)
12643862 .. 12644326
465 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g21030.t1

Sequence Viewer

Length: 465 bp
ATGAAGTGTTTCTTCCTTATTGTCATCTTTCTCGTGCTCTTTCACACCACATTCGGTGATGACTTAATCCAACAATCTTGCAAGAAAGCTGCAGATCGCGAACGATCTTTGAACCACACCTTCTGTGTCTCAACCCTTGAGGCCGACCCCAAAAGCCGTGGCGCAGACCTTTATGGACTTGCTAATATCACACTTTACCTAGCCGGAACCAAGGTTATTAAGACTGCGTTACAAGACTGCGAAGATGCCCCTGATAGCATGCAGGAAGCTATCTATTCGTTCAAGGAGAAGGATTACGAGGCTGCTCTTAATCCTCTCAGTGCTGCCTTAAACGCACCTGATACTTGCGAAACTGGGTTTCAAGAGAGGAAAGCAGTGTCTCCCTTGAAGAAAGAGGACGATGATTTCACTCAATTGGGTGTCGTATCTCTTACTTTCATTGGCCTGTTAAGTGGGAAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

155

Amino Acids

16.97

Weight (kDa)

4.88

Isoelectric Point (pI)

25.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 21 - 144 7e-13 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 99
AgsI TTSAA 4 cut(s) 112, 283, 362, 388
AluBI AGCT 2 cut(s) 89, 269
AluI AGCT 2 cut(s) 89, 269
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 2 cut(s) 133, 384
AoxI GGCC 2 cut(s) 141, 442
ApeKI GCWGC 3 cut(s) 89, 302, 323
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 1 cut(s) 164
AsuHPI GGTGA 1 cut(s) 68
BauI CACGAG 1 cut(s) 32
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 3 cut(s) 76, 289, 310
BceAI ACGGC 1 cut(s) 141
BcoDI GTCTC 2 cut(s) 133, 384
BfaI CTAG 1 cut(s) 200
BfmI CTRYAG 1 cut(s) 90
BisI GCNGC 3 cut(s) 90, 303, 324
BlsI GCNGC 3 cut(s) 91, 304, 325
BmiI GGNNCC 1 cut(s) 208
BmrI ACTGGG 1 cut(s) 363
BmsI GCATC 1 cut(s) 235
BmuI ACTGGG 1 cut(s) 363
BpuEI CTTGAG 1 cut(s) 158
BsaJI CCNNGG 2 cut(s) 157, 210
Bse1I ACTGG 1 cut(s) 358
BseDI CCNNGG 2 cut(s) 157, 210
BseMII CTCAG 1 cut(s) 331
BseNI ACTGG 1 cut(s) 358
BseXI GCAGC 3 cut(s) 76, 289, 310
Bsh1236I CGCG 1 cut(s) 99
BshFI GGCC 2 cut(s) 143, 444
BsiHKAI GWGCWC 1 cut(s) 39
BsiSI CCGG 1 cut(s) 204
BsmAI GTCTC 2 cut(s) 133, 384
BsnI GGCC 2 cut(s) 143, 444
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 2 cut(s) 94, 104
Bsp68I TCGCGA 1 cut(s) 99
BspANI GGCC 2 cut(s) 143, 444
BspCNI CTCAG 1 cut(s) 330
BspFNI CGCG 1 cut(s) 99
BspLI GGNNCC 1 cut(s) 208
BspMAI CTGCAG 1 cut(s) 94
BsrI ACTGG 1 cut(s) 358
BssECI CCNNGG 2 cut(s) 157, 210
BssMI GATC 2 cut(s) 94, 104
BssSI CACGAG 1 cut(s) 32
BssT1I CCWWGG 1 cut(s) 210
Bst2BI CACGAG 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 260
BstDEI CTNAG 1 cut(s) 317
BstDSI CCRYGG 1 cut(s) 157
BstFNI CGCG 1 cut(s) 99
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 2 cut(s) 97, 107
BstMAI GTCTC 2 cut(s) 133, 384
BstMBI GATC 2 cut(s) 94, 104
BstMWI GCNNNNNNNGC 1 cut(s) 332
BstNSI RCATGY 1 cut(s) 262
BstSFI CTRYAG 1 cut(s) 90
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 3 cut(s) 76, 289, 310
BsuRI GGCC 2 cut(s) 143, 444
BtgI CCRYGG 1 cut(s) 157
BtsI GCAGTG 1 cut(s) 381
BtsIMutI CAGTG 2 cut(s) 325, 381
BtuMI TCGCGA 1 cut(s) 99
Cac8I GCNNGC 1 cut(s) 260
CfoI GCGC 1 cut(s) 164
CspCI CAANNNNNGTGG 2 cut(s) 139, 174
CviAII CATG 2 cut(s) 259, 462
CviJI RGCY 7 cut(s) 89, 143, 156, 203, 269, 302, 444
CviKI_1 RGCY 7 cut(s) 89, 143, 156, 203, 269, 302, 444
DdeI CTNAG 1 cut(s) 317
DpnI GATC 2 cut(s) 96, 106
DpnII GATC 2 cut(s) 94, 104
Eco130I CCWWGG 1 cut(s) 210
EcoT14I CCWWGG 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 210
FaeI CATG 2 cut(s) 262, 465
FaiI YATR 3 cut(s) 174, 260, 463
FalI AAGNNNNNCTT 1 cut(s) 28
FatI CATG 2 cut(s) 258, 461
Fnu4HI GCNGC 3 cut(s) 90, 303, 324
Fsp4HI GCNGC 3 cut(s) 90, 303, 324
FspBI CTAG 1 cut(s) 200
GlaI GCGC 1 cut(s) 163
GluI GCNGC 3 cut(s) 90, 303, 324
HaeIII GGCC 2 cut(s) 143, 444
HapII CCGG 1 cut(s) 204
HhaI GCGC 1 cut(s) 164
Hin1II CATG 2 cut(s) 262, 465
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HpaII CCGG 1 cut(s) 204
HphI GGTGA 1 cut(s) 68
Hpy188III TCNNGA 2 cut(s) 98, 362
HpyAV CCTTC 2 cut(s) 130, 283
HpyCH4V TGCA 3 cut(s) 81, 92, 262
HpyF10VI GCNNNNNNNGC 1 cut(s) 332
HpyF3I CTNAG 1 cut(s) 317
Hsp92II CATG 2 cut(s) 262, 465
HspAI GCGC 1 cut(s) 162
Kzo9I GATC 2 cut(s) 94, 104
LpnPI CCDG 6 cut(s) 217, 248, 264, 339, 351, 458
Lsp1109I GCAGC 3 cut(s) 76, 289, 310
LweI GCATC 1 cut(s) 235
MaeI CTAG 1 cut(s) 200
MaeIII GTNAC 1 cut(s) 228
MalI GATC 2 cut(s) 96, 106
MboI GATC 2 cut(s) 94, 104
MboII GAAGA 3 cut(s) 4, 254, 400
MfeI CAATTG 1 cut(s) 413
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 1 cut(s) 413
MmeI TCCRAC 1 cut(s) 94
MnlI CCTC 5 cut(s) 133, 292, 324, 360, 388
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 5 cut(s) 65, 219, 309, 329, 449
MspI CCGG 1 cut(s) 204
MunI CAATTG 1 cut(s) 413
MvnI CGCG 1 cut(s) 99
MwoI GCNNNNNNNGC 1 cut(s) 332
NdeII GATC 2 cut(s) 94, 104
NlaIII CATG 2 cut(s) 262, 465
NlaIV GGNNCC 1 cut(s) 208
NruI TCGCGA 1 cut(s) 99
NspI RCATGY 1 cut(s) 262
PaeI GCATGC 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 8
PkrI GCNGC 3 cut(s) 91, 304, 325
PspN4I GGNNCC 1 cut(s) 208
PstI CTGCAG 1 cut(s) 94
RruI TCGCGA 1 cut(s) 99
SaqAI TTAA 5 cut(s) 65, 219, 309, 329, 449
SatI GCNGC 3 cut(s) 90, 303, 324
Sau3AI GATC 2 cut(s) 94, 104
SduI GDGCHC 1 cut(s) 39
SetI ASST 7 cut(s) 91, 122, 171, 201, 216, 271, 340
SfaNI GCATC 1 cut(s) 235
SfcI CTRYAG 1 cut(s) 90
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
SphI GCATGC 1 cut(s) 262
Sse9I AATT 1 cut(s) 413
SspMI CTAG 1 cut(s) 200
StyI CCWWGG 1 cut(s) 210
TasI AATT 1 cut(s) 413
Tru1I TTAA 5 cut(s) 65, 219, 309, 329, 449
Tru9I TTAA 5 cut(s) 65, 219, 309, 329, 449
TscAI CASTG 2 cut(s) 325, 381
TseI GCWGC 3 cut(s) 89, 302, 323
TspDTI ATGAA 2 cut(s) 17, 427
TspRI CASTG 2 cut(s) 325, 381
XceI RCATGY 1 cut(s) 262
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.