RchiOBHm_Chr7g0207981

invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002424
Physical Location & Seq
Forward (+)
25329218 .. 25329736
519 bp
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UTR
Exon/CDS
Intron
PRQ18613

Sequence Viewer

Length: 519 bp
ATGAGGTATCTGATCTTCCTCGTTACTTTTCTCGTGCTCTGTCACAGCACAATGGGAGATGATCTCGTCCAACAAACTTGCAAGAAAGCCTCAGATGCTGATAAAAACTTGAACTACAACTTTTGTGTCTCAGCCCTTGAGGCCAGCCCGAAAAGCAAGGGTGCAGACCTCGCAGGACTAGCTGGCATTGCACTTGACCTTACCACAGCGAAGGCGACAAGCATCAAGTCTACCATTTCAAAGCTCTTGAACGATCCAAAAGTTGACAAGCCTGCGCTACAAGACTGCGAAGAACTCTATTCAGGTGCCACTGATGACCTGCAAGCAGCCACCAATGCTTTCAAGTCCAGTGATTATGGTACAGCCCTTACACATCTGAGTGCTACCTTGAATGCACCTGATACTTGTGAAACCGGGTTCCAAGAGAGGAAAGCAGTGTCTCCTTTGAGCAAGGACGACGGTGACTTCGCTCAGTTGAACATCATATCTCTTGCCTTTATCAACCAATTGCGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

172

Amino Acids

18.39

Weight (kDa)

4.93

Isoelectric Point (pI)

18.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 21 - 165 4.7e-28 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 327
AccB1I GGYRCC 1 cut(s) 305
AccI GTMKAC 1 cut(s) 230
AclWI GGATC 1 cut(s) 248
AfaI GTAC 1 cut(s) 361
AgsI TTSAA 6 cut(s) 112, 240, 250, 343, 391, 478
AluBI AGCT 2 cut(s) 182, 244
AluI AGCT 2 cut(s) 182, 244
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 2 cut(s) 133, 444
AlwI GGATC 1 cut(s) 248
AlwNI CAGNNNCTG 1 cut(s) 98
AoxI GGCC 1 cut(s) 141
ApeKI GCWGC 1 cut(s) 326
ArsI GACNNNNNNTTYG 2 cut(s) 449, 481
AspLEI GCGC 1 cut(s) 277
AsuC2I CCSGG 1 cut(s) 415
AsuHPI GGTGA 1 cut(s) 473
BanI GGYRCC 1 cut(s) 305
BarI GAAGNNNNNNTAC 1 cut(s) 31
BauI CACGAG 1 cut(s) 32
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 1 cut(s) 338
BcnI CCSGG 1 cut(s) 415
BcoDI GTCTC 2 cut(s) 133, 444
BfaI CTAG 1 cut(s) 179
BfuAI ACCTGC 1 cut(s) 327
BglI GCCNNNNNGGC 1 cut(s) 140
BisI GCNGC 1 cut(s) 327
BlsI GCNGC 1 cut(s) 328
Bme1390I CCNGG 1 cut(s) 415
BmiI GGNNCC 2 cut(s) 307, 419
BmrFI CCNGG 1 cut(s) 415
BmsI GCATC 2 cut(s) 85, 231
BplI GAGNNNNNCTC 2 cut(s) 48, 80
BpuEI CTTGAG 1 cut(s) 158
BpuMI CCSGG 1 cut(s) 415
Bse1I ACTGG 1 cut(s) 348
Bse3DI GCAATG 1 cut(s) 186
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 4 cut(s) 105, 144, 368, 485
BseNI ACTGG 1 cut(s) 348
BseXI GCAGC 1 cut(s) 338
BsgI GTGCAG 1 cut(s) 183
BshFI GGCC 1 cut(s) 143
BshNI GGYRCC 1 cut(s) 305
BsiHKAI GWGCWC 1 cut(s) 39
BsiSI CCGG 1 cut(s) 414
BsmAI GTCTC 2 cut(s) 133, 444
BsmI GAATGC 1 cut(s) 397
BsnI GGCC 1 cut(s) 143
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 3 cut(s) 12, 61, 253
BspANI GGCC 1 cut(s) 143
BspCNI CTCAG 4 cut(s) 104, 143, 369, 484
BspLI GGNNCC 2 cut(s) 307, 419
BspMI ACCTGC 1 cut(s) 327
BspPI GGATC 1 cut(s) 248
BspT107I GGYRCC 1 cut(s) 305
BsrDI GCAATG 1 cut(s) 186
BsrI ACTGG 1 cut(s) 348
BssMI GATC 3 cut(s) 12, 61, 253
BssSI CACGAG 1 cut(s) 32
Bst2BI CACGAG 1 cut(s) 32
Bst4CI ACNGT 1 cut(s) 461
BstC8I GCNNGC 4 cut(s) 145, 184, 273, 324
BstDEI CTNAG 4 cut(s) 91, 130, 377, 471
BstHHI GCGC 1 cut(s) 277
BstKTI GATC 3 cut(s) 15, 64, 256
BstMAI GTCTC 2 cut(s) 133, 444
BstMBI GATC 3 cut(s) 12, 61, 253
BstMWI GCNNNNNNNGC 7 cut(s) 95, 140, 153, 170, 179, 188, 335
BstSCI CCNGG 1 cut(s) 413
BstV1I GCAGC 1 cut(s) 338
BstXI CCANNNNNNTGG 1 cut(s) 512
BsuRI GGCC 1 cut(s) 143
BtsI GCAGTG 1 cut(s) 441
BtsIMutI CAGTG 3 cut(s) 309, 355, 441
BveI ACCTGC 1 cut(s) 327
Cac8I GCNNGC 4 cut(s) 145, 184, 273, 324
CaiI CAGNNNCTG 1 cut(s) 98
CfoI GCGC 1 cut(s) 277
Csp6I GTAC 1 cut(s) 360
CviJI RGCY 9 cut(s) 89, 134, 143, 147, 182, 244, 271, 329, 365
CviKI_1 RGCY 9 cut(s) 89, 134, 143, 147, 182, 244, 271, 329, 365
CviQI GTAC 1 cut(s) 360
DdeI CTNAG 4 cut(s) 91, 130, 377, 471
DpnI GATC 3 cut(s) 14, 63, 255
DpnII GATC 3 cut(s) 12, 61, 253
FaiI YATR 2 cut(s) 357, 485
FblI GTMKAC 1 cut(s) 230
Fnu4HI GCNGC 1 cut(s) 327
Fsp4HI GCNGC 1 cut(s) 327
FspBI CTAG 1 cut(s) 179
GlaI GCGC 1 cut(s) 276
GluI GCNGC 1 cut(s) 327
HaeIII GGCC 1 cut(s) 143
HapII CCGG 1 cut(s) 414
HhaI GCGC 1 cut(s) 277
Hin6I GCGC 1 cut(s) 275
HinP1I GCGC 1 cut(s) 275
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HpaII CCGG 1 cut(s) 414
HphI GGTGA 1 cut(s) 473
Hpy166II GTNNAC 2 cut(s) 231, 265
Hpy188I TCNGA 3 cut(s) 12, 94, 378
Hpy188III TCNNGA 1 cut(s) 247
Hpy8I GTNNAC 2 cut(s) 231, 265
Hpy99I CGWCG 1 cut(s) 461
HpyAV CCTTC 1 cut(s) 205
HpyCH4III ACNGT 1 cut(s) 461
HpyCH4V TGCA 5 cut(s) 81, 164, 191, 322, 395
HpyF10VI GCNNNNNNNGC 7 cut(s) 95, 140, 153, 170, 179, 188, 335
HpyF3I CTNAG 4 cut(s) 91, 130, 377, 471
HspAI GCGC 1 cut(s) 275
Kzo9I GATC 3 cut(s) 12, 61, 253
LpnPI CCDG 9 cut(s) 157, 159, 168, 285, 288, 332, 361, 411, 427
Lsp1109I GCAGC 1 cut(s) 338
LweI GCATC 2 cut(s) 85, 231
MaeI CTAG 1 cut(s) 179
MaeIII GTNAC 3 cut(s) 22, 41, 461
MalI GATC 3 cut(s) 14, 63, 255
MboI GATC 3 cut(s) 12, 61, 253
MboII GAAGA 2 cut(s) 7, 302
MfeI CAATTG 1 cut(s) 506
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 1 cut(s) 506
MmeI TCCRAC 1 cut(s) 94
MnlI CCTC 5 cut(s) 29, 100, 133, 179, 420
MslI CAYNNNNRTG 1 cut(s) 378
MspI CCGG 1 cut(s) 414
MspR9I CCNGG 1 cut(s) 415
MunI CAATTG 1 cut(s) 506
Mva1269I GAATGC 1 cut(s) 397
MwoI GCNNNNNNNGC 7 cut(s) 95, 140, 153, 170, 179, 188, 335
NciI CCSGG 1 cut(s) 415
NdeII GATC 3 cut(s) 12, 61, 253
NlaIV GGNNCC 2 cut(s) 307, 419
NmuCI GTSAC 2 cut(s) 41, 461
PctI GAATGC 1 cut(s) 397
PkrI GCNGC 1 cut(s) 328
PspN4I GGNNCC 2 cut(s) 307, 419
PstNI CAGNNNCTG 1 cut(s) 98
RsaI GTAC 1 cut(s) 361
RsaNI GTAC 1 cut(s) 360
RseI CAYNNNNRTG 1 cut(s) 378
SatI GCNGC 1 cut(s) 327
Sau3AI GATC 3 cut(s) 12, 61, 253
ScrFI CCNGG 1 cut(s) 415
SduI GDGCHC 1 cut(s) 39
SetI ASST 9 cut(s) 8, 171, 184, 201, 246, 307, 321, 389, 400
SfaNI GCATC 2 cut(s) 85, 231
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 1 cut(s) 506
SspMI CTAG 1 cut(s) 179
StyD4I CCNGG 1 cut(s) 413
TaaI ACNGT 1 cut(s) 461
TasI AATT 1 cut(s) 506
TscAI CASTG 3 cut(s) 316, 355, 441
TseFI GTSAC 2 cut(s) 41, 461
TseI GCWGC 1 cut(s) 326
Tsp45I GTSAC 2 cut(s) 41, 461
TspRI CASTG 3 cut(s) 316, 355, 441
XmiI GTMKAC 1 cut(s) 230
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.