AT5G46950

invertase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002424
Physical Location & Seq
Reverse (-)
19059472 .. 19060121
650 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46950.1

Sequence Viewer

Length: 525 bp
ATGAAGTTCTTGGTTTCATTGGTGATCTTCTCTCTTTTCTTGAACGGTTTCGCAACCGCTCAAACTCTCATTCAAGATTCTTGCAAGAAAGCTTTTGCGAAAGACCCGCAATCGTCATACGATTTCTGCGTCCAATCTCTTACGCAAGATCCACAAAGCAAAGCCGCAACTACTCTCGAAGATTTGGCCCTAGCATCGACAAAGAACGTTGCGGCAAAAATCACGAACCTGAAAGGAATCGTTGCACAGGATCTCAAGGACCAGAGATATCAGGATATTGTGGAAGACTTAAAACTTTGCCTCGGATTTTATAAAGATGCTAATGATTCTTTAAAAACTGCTTTAGCGAACATTAAATCGCGTGATTATGACGGCGCTAACAGTAATCTGAGTGCTGCTTTGAATGCTCCAGGCGATTGCGAGGATGATTTCAAGGAAGCAGAAAAGAAGTCTCCGATTACTAACGAGAACAATATTTTGTATAAGACGATTGTAATTCCTTTGGCTTTTACTACTATGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

174

Amino Acids

19.09

Weight (kDa)

4.99

Isoelectric Point (pI)

37.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 23 - 169 1.8e-23 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 312
AccBSI CCGCTC 1 cut(s) 59
AccII CGCG 1 cut(s) 361
AciI CCGC 4 cut(s) 57, 107, 165, 212
AclI AACGTT 1 cut(s) 207
AclWI GGATC 2 cut(s) 143, 258
AgsI TTSAA 4 cut(s) 43, 74, 403, 433
AjnI CCWGG 1 cut(s) 409
AluBI AGCT 1 cut(s) 92
AluI AGCT 1 cut(s) 92
Alw26I GTCTC 1 cut(s) 456
AlwI GGATC 2 cut(s) 143, 258
AoxI GGCC 1 cut(s) 186
ApeKI GCWGC 1 cut(s) 395
Asp700I GAANNNNTTC 1 cut(s) 47
AspLEI GCGC 1 cut(s) 377
AspS9I GGNCC 2 cut(s) 187, 259
AsuHPI GGTGA 1 cut(s) 34
AvaII GGWCC 1 cut(s) 259
BbsI GAAGAC 1 cut(s) 291
BbvI GCAGC 1 cut(s) 382
BceAI ACGGC 1 cut(s) 388
BciT130I CCWGG 1 cut(s) 411
BcoDI GTCTC 1 cut(s) 456
BfaI CTAG 1 cut(s) 191
BfoI RGCGCY 1 cut(s) 378
BisI GCNGC 3 cut(s) 165, 213, 396
BlsI GCNGC 3 cut(s) 166, 214, 397
Bme1390I CCNGG 1 cut(s) 411
Bme18I GGWCC 1 cut(s) 259
BmgT120I GGNCC 2 cut(s) 187, 259
BmrFI CCNGG 1 cut(s) 411
BmsI GCATC 2 cut(s) 203, 307
BpiI GAAGAC 1 cut(s) 291
BpmI CTGGAG 1 cut(s) 393
BpuEI CTTGAG 1 cut(s) 239
BsaJI CCNNGG 1 cut(s) 301
BseBI CCWGG 1 cut(s) 411
BseDI CCNNGG 1 cut(s) 301
BseGI GGATG 1 cut(s) 430
BseMII CTCAG 1 cut(s) 380
BseXI GCAGC 1 cut(s) 382
Bsh1236I CGCG 1 cut(s) 361
BshFI GGCC 1 cut(s) 188
BsmAI GTCTC 1 cut(s) 456
BsmI GAATGC 1 cut(s) 409
BsnI GGCC 1 cut(s) 188
Bsp143I GATC 3 cut(s) 24, 148, 250
BspACI CCGC 4 cut(s) 57, 107, 165, 212
BspANI GGCC 1 cut(s) 188
BspCNI CTCAG 1 cut(s) 381
BspFNI CGCG 1 cut(s) 361
BspPI GGATC 2 cut(s) 143, 258
BsrBI CCGCTC 1 cut(s) 59
BssECI CCNNGG 1 cut(s) 301
BssMI GATC 3 cut(s) 24, 148, 250
Bst2UI CCWGG 1 cut(s) 411
Bst4CI ACNGT 2 cut(s) 47, 383
BstDEI CTNAG 1 cut(s) 389
BstF5I GGATG 1 cut(s) 430
BstFNI CGCG 1 cut(s) 361
BstH2I RGCGCY 1 cut(s) 378
BstHHI GCGC 1 cut(s) 377
BstKTI GATC 3 cut(s) 27, 151, 253
BstMAI GTCTC 1 cut(s) 456
BstMBI GATC 3 cut(s) 24, 148, 250
BstMWI GCNNNNNNNGC 1 cut(s) 404
BstNI CCWGG 1 cut(s) 411
BstSCI CCNGG 1 cut(s) 409
BstUI CGCG 1 cut(s) 361
BstV1I GCAGC 1 cut(s) 382
BstV2I GAAGAC 1 cut(s) 291
BstX2I RGATCY 2 cut(s) 148, 250
BstYI RGATCY 2 cut(s) 148, 250
BsuRI GGCC 1 cut(s) 188
BtsCI GGATG 1 cut(s) 430
CfoI GCGC 1 cut(s) 377
Cfr13I GGNCC 2 cut(s) 187, 259
CseI GACGC 1 cut(s) 118
CviJI RGCY 4 cut(s) 92, 164, 188, 506
CviKI_1 RGCY 4 cut(s) 92, 164, 188, 506
DdeI CTNAG 1 cut(s) 389
DpnI GATC 3 cut(s) 26, 150, 252
DpnII GATC 3 cut(s) 24, 148, 250
DraI TTTAAA 1 cut(s) 333
Eco32I GATATC 1 cut(s) 269
Eco47I GGWCC 1 cut(s) 259
EcoRII CCWGG 1 cut(s) 409
EcoRV GATATC 1 cut(s) 269
FaiI YATR 5 cut(s) 118, 312, 369, 483, 518
FauI CCCGC 1 cut(s) 114
Fnu4HI GCNGC 3 cut(s) 165, 213, 396
FokI GGATG 1 cut(s) 437
Fsp4HI GCNGC 3 cut(s) 165, 213, 396
FspBI CTAG 1 cut(s) 191
GlaI GCGC 1 cut(s) 376
GluI GCNGC 3 cut(s) 165, 213, 396
GsuI CTGGAG 1 cut(s) 393
HaeII RGCGCY 1 cut(s) 378
HaeIII GGCC 1 cut(s) 188
HgaI GACGC 1 cut(s) 118
HhaI GCGC 1 cut(s) 377
Hin6I GCGC 1 cut(s) 375
HinP1I GCGC 1 cut(s) 375
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 3 cut(s) 77, 237, 326
HphI GGTGA 1 cut(s) 34
Hpy188I TCNGA 3 cut(s) 305, 390, 456
Hpy188III TCNNGA 5 cut(s) 40, 74, 176, 223, 272
HpyCH4III ACNGT 2 cut(s) 47, 383
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 2 cut(s) 84, 245
HpyF10VI GCNNNNNNNGC 1 cut(s) 404
HpyF3I CTNAG 1 cut(s) 389
HpySE526I ACGT 1 cut(s) 207
HspAI GCGC 1 cut(s) 375
Kzo9I GATC 3 cut(s) 24, 148, 250
LmnI GCTCC 1 cut(s) 412
LpnPI CCDG 6 cut(s) 233, 242, 257, 275, 396, 423
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 2 cut(s) 203, 307
MaeI CTAG 1 cut(s) 191
MaeII ACGT 1 cut(s) 207
MalI GATC 3 cut(s) 26, 150, 252
MbiI CCGCTC 1 cut(s) 59
MboI GATC 3 cut(s) 24, 148, 250
MboII GAAGA 3 cut(s) 19, 191, 296
MflI RGATCY 2 cut(s) 148, 250
MluCI AATT 1 cut(s) 495
MnlI CCTC 2 cut(s) 311, 415
MroXI GAANNNNTTC 1 cut(s) 47
MseI TTAA 3 cut(s) 290, 332, 354
MspR9I CCNGG 1 cut(s) 411
Mva1269I GAATGC 1 cut(s) 409
MvaI CCWGG 1 cut(s) 411
MvnI CGCG 1 cut(s) 361
MwoI GCNNNNNNNGC 1 cut(s) 404
NdeII GATC 3 cut(s) 24, 148, 250
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PctI GAATGC 1 cut(s) 409
PdmI GAANNNNTTC 1 cut(s) 47
PfeI GAWTC 3 cut(s) 77, 237, 326
PkrI GCNGC 3 cut(s) 166, 214, 397
PsiI TTATAA 1 cut(s) 312
Psp1406I AACGTT 1 cut(s) 207
Psp6I CCWGG 1 cut(s) 409
PspGI CCWGG 1 cut(s) 409
PspPI GGNCC 2 cut(s) 187, 259
PsuI RGATCY 2 cut(s) 148, 250
SaqAI TTAA 3 cut(s) 290, 332, 354
SatI GCNGC 3 cut(s) 165, 213, 396
Sau3AI GATC 3 cut(s) 24, 148, 250
Sau96I GGNCC 2 cut(s) 187, 259
ScrFI CCNGG 1 cut(s) 411
SetI ASST 3 cut(s) 94, 210, 231
SfaNI GCATC 2 cut(s) 203, 307
SinI GGWCC 1 cut(s) 259
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 1 cut(s) 495
SsiI CCGC 4 cut(s) 57, 107, 165, 212
SspI AATATT 1 cut(s) 475
SspMI CTAG 1 cut(s) 191
StyD4I CCNGG 1 cut(s) 409
TaaI ACNGT 2 cut(s) 47, 383
TaiI ACGT 1 cut(s) 210
TaqI TCGA 2 cut(s) 177, 197
TasI AATT 1 cut(s) 495
TauI GCSGC 2 cut(s) 167, 215
TfiI GAWTC 3 cut(s) 77, 237, 326
Tru1I TTAA 3 cut(s) 290, 332, 354
Tru9I TTAA 3 cut(s) 290, 332, 354
TseI GCWGC 1 cut(s) 395
TspDTI ATGAA 2 cut(s) 6, 17
VpaK11BI GGWCC 1 cut(s) 259
XmnI GAANNNNTTC 1 cut(s) 47
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.