RLG00000003264

invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0002424
Physical Location & Seq
Reverse (-)
46329390 .. 46329920
531 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003264

Sequence Viewer

Length: 531 bp
ATGAGGTTCCTCTTCTTTATCACTTTTCTCGTGCTCCTTCGCAACACTACGGGTTGCAACGATCTCATCCATCTCTCCTGCAAGAAAGCTGCAGCTAGTGATCCCAATTTGAGCTACAACTTCTGTGTTTCAAGCCTTGAGGCCATCCCCAAAAGCCATGGAGCAGACCTTGAGCAACTTGTGGCCCTTACACTTAACCTTACCATATCCAACGCAACCAGCATCAATTCCACCATTTCGAAGCTTTTGCTCGATAAAAAATTCGACAGGTATGCAAAGGAGTGCTTGCAAGACTGCTTAGAGCTGTATTCGGATGCCGTTCCTACCCTACAAGAAGCTGTTGGTGCCTTTAAGGCCAAGGATTATGGTAAAGCTAACATAGAAATCAGTGCTGCCATGGATGCATCTAGCACTTGTGAAGACGGGTTCATGGAGAAGAAAGGTGAAGTTTATCCATTAAAAAGGGAGAATGATAACTTCAGCCAATTGAACGTCATCTCTCTTGCTTTTATCAACACATTAGGTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

177

Amino Acids

19.4

Weight (kDa)

5.36

Isoelectric Point (pI)

30.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 21 - 169 8.3e-29 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 344
AclWI GGATC 1 cut(s) 95
AcsI RAATTY 1 cut(s) 260
AcuI CTGAAG 1 cut(s) 463
AgsI TTSAA 2 cut(s) 132, 490
AluBI AGCT 7 cut(s) 89, 95, 114, 244, 304, 338, 374
AluI AGCT 7 cut(s) 89, 95, 114, 244, 304, 338, 374
Alw21I GWGCWC 1 cut(s) 36
AlwI GGATC 1 cut(s) 95
AoxI GGCC 3 cut(s) 141, 183, 354
ApeKI GCWGC 3 cut(s) 89, 92, 392
ApoI RAATTY 1 cut(s) 260
AspS9I GGNCC 1 cut(s) 184
AsuHPI GGTGA 1 cut(s) 455
AsuII TTCGAA 1 cut(s) 239
BanI GGYRCC 1 cut(s) 344
BauI CACGAG 1 cut(s) 29
BbsI GAAGAC 1 cut(s) 426
Bbv12I GWGCWC 1 cut(s) 36
BbvI GCAGC 3 cut(s) 76, 104, 379
BccI CCATC 2 cut(s) 78, 152
BceAI ACGGC 1 cut(s) 302
BcgI CGANNNNNNTGC 4 cut(s) 229, 254, 263, 288
BfaI CTAG 2 cut(s) 96, 408
BfmI CTRYAG 1 cut(s) 90
BglI GCCNNNNNGGC 1 cut(s) 353
BisI GCNGC 3 cut(s) 90, 93, 393
BlsI GCNGC 3 cut(s) 91, 94, 394
BmgT120I GGNCC 1 cut(s) 184
BmiI GGNNCC 2 cut(s) 8, 346
BmsI GCATC 4 cut(s) 231, 304, 391, 413
BpiI GAAGAC 1 cut(s) 426
Bpu14I TTCGAA 1 cut(s) 239
BpuEI CTTGAG 2 cut(s) 158, 191
BsaJI CCNNGG 3 cut(s) 157, 357, 396
BseDI CCNNGG 3 cut(s) 157, 357, 396
BseGI GGATG 4 cut(s) 66, 144, 319, 406
BseXI GCAGC 3 cut(s) 76, 104, 379
BshFI GGCC 3 cut(s) 143, 185, 356
BshNI GGYRCC 1 cut(s) 344
BsiHKAI GWGCWC 1 cut(s) 36
BsnI GGCC 3 cut(s) 143, 185, 356
Bsp119I TTCGAA 1 cut(s) 239
Bsp1286I GDGCHC 1 cut(s) 36
Bsp143I GATC 2 cut(s) 61, 100
Bsp19I CCATGG 2 cut(s) 157, 396
BspANI GGCC 3 cut(s) 143, 185, 356
BspLI GGNNCC 2 cut(s) 8, 346
BspMAI CTGCAG 1 cut(s) 94
BspPI GGATC 1 cut(s) 95
BspT104I TTCGAA 1 cut(s) 239
BspT107I GGYRCC 1 cut(s) 344
BssECI CCNNGG 3 cut(s) 157, 357, 396
BssMI GATC 2 cut(s) 61, 100
BssSI CACGAG 1 cut(s) 29
BssT1I CCWWGG 3 cut(s) 157, 357, 396
Bst2BI CACGAG 1 cut(s) 29
Bst6I CTCTTC 1 cut(s) 17
BstBI TTCGAA 1 cut(s) 239
BstC8I GCNNGC 1 cut(s) 287
BstDEI CTNAG 1 cut(s) 298
BstDSI CCRYGG 2 cut(s) 157, 396
BstF5I GGATG 4 cut(s) 66, 144, 319, 406
BstKTI GATC 2 cut(s) 64, 103
BstMBI GATC 2 cut(s) 61, 100
BstMWI GCNNNNNNNGC 3 cut(s) 344, 353, 401
BstSFI CTRYAG 1 cut(s) 90
BstV1I GCAGC 3 cut(s) 76, 104, 379
BstV2I GAAGAC 1 cut(s) 426
BsuRI GGCC 3 cut(s) 143, 185, 356
BtgI CCRYGG 2 cut(s) 157, 396
BtsCI GGATG 4 cut(s) 66, 144, 319, 406
BtsIMutI CAGTG 1 cut(s) 394
Cac8I GCNNGC 1 cut(s) 287
Cfr13I GGNCC 1 cut(s) 184
CviAII CATG 3 cut(s) 158, 397, 430
DdeI CTNAG 1 cut(s) 298
DpnI GATC 2 cut(s) 63, 102
DpnII GATC 2 cut(s) 61, 100
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Eco130I CCWWGG 3 cut(s) 157, 357, 396
Eco57I CTGAAG 1 cut(s) 463
EcoT14I CCWWGG 3 cut(s) 157, 357, 396
EcoT22I ATGCAT 1 cut(s) 406
ErhI CCWWGG 3 cut(s) 157, 357, 396
FaeI CATG 3 cut(s) 161, 400, 433
FaiI YATR 7 cut(s) 159, 206, 273, 366, 380, 398, 431
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FatI CATG 3 cut(s) 157, 396, 429
Fnu4HI GCNGC 3 cut(s) 90, 93, 393
FokI GGATG 4 cut(s) 53, 131, 326, 413
Fsp4HI GCNGC 3 cut(s) 90, 93, 393
FspBI CTAG 2 cut(s) 96, 408
GluI GCNGC 3 cut(s) 90, 93, 393
HaeIII GGCC 3 cut(s) 143, 185, 356
Hin1II CATG 3 cut(s) 161, 400, 433
HindIII AAGCTT 1 cut(s) 242
HphI GGTGA 1 cut(s) 455
Hpy188I TCNGA 1 cut(s) 313
HpyAV CCTTC 1 cut(s) 47
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 6 cut(s) 57, 81, 92, 275, 289, 404
HpyF10VI GCNNNNNNNGC 3 cut(s) 344, 353, 401
HpyF3I CTNAG 1 cut(s) 298
HpySE526I ACGT 1 cut(s) 492
Hsp92II CATG 3 cut(s) 161, 400, 433
Kzo9I GATC 2 cut(s) 61, 100
LmnI GCTCC 2 cut(s) 39, 161
LpnPI CCDG 3 cut(s) 91, 232, 253
Lsp1109I GCAGC 3 cut(s) 76, 104, 379
LweI GCATC 4 cut(s) 231, 304, 391, 413
MaeI CTAG 2 cut(s) 96, 408
MaeII ACGT 1 cut(s) 492
MalI GATC 2 cut(s) 63, 102
MboI GATC 2 cut(s) 61, 100
MboII GAAGA 3 cut(s) 4, 431, 448
MfeI CAATTG 1 cut(s) 485
MhlI GDGCHC 1 cut(s) 36
MluCI AATT 4 cut(s) 106, 226, 260, 485
MmeI TCCRAC 1 cut(s) 234
MnlI CCTC 2 cut(s) 20, 133
Mph1103I ATGCAT 1 cut(s) 406
MseI TTAA 3 cut(s) 195, 351, 458
MunI CAATTG 1 cut(s) 485
MwoI GCNNNNNNNGC 3 cut(s) 344, 353, 401
NcoI CCATGG 2 cut(s) 157, 396
NdeII GATC 2 cut(s) 61, 100
NlaIII CATG 3 cut(s) 161, 400, 433
NlaIV GGNNCC 2 cut(s) 8, 346
NsiI ATGCAT 1 cut(s) 406
NspV TTCGAA 1 cut(s) 239
PkrI GCNGC 3 cut(s) 91, 94, 394
PspN4I GGNNCC 2 cut(s) 8, 346
PspPI GGNCC 1 cut(s) 184
PstI CTGCAG 1 cut(s) 94
SaqAI TTAA 3 cut(s) 195, 351, 458
SatI GCNGC 3 cut(s) 90, 93, 393
Sau3AI GATC 2 cut(s) 61, 100
Sau96I GGNCC 1 cut(s) 184
SduI GDGCHC 1 cut(s) 36
SfaNI GCATC 4 cut(s) 231, 304, 391, 413
SfcI CTRYAG 1 cut(s) 90
SfuI TTCGAA 1 cut(s) 239
SmlI CTYRAG 2 cut(s) 137, 170
SmoI CTYRAG 2 cut(s) 137, 170
Sse9I AATT 4 cut(s) 106, 226, 260, 485
SspMI CTAG 2 cut(s) 96, 408
StyI CCWWGG 3 cut(s) 157, 357, 396
TaiI ACGT 1 cut(s) 495
TaqI TCGA 3 cut(s) 239, 252, 264
TasI AATT 4 cut(s) 106, 226, 260, 485
Tru1I TTAA 3 cut(s) 195, 351, 458
Tru9I TTAA 3 cut(s) 195, 351, 458
TscAI CASTG 1 cut(s) 394
TseI GCWGC 3 cut(s) 89, 92, 392
TspDTI ATGAA 1 cut(s) 418
TspRI CASTG 1 cut(s) 394
XapI RAATTY 1 cut(s) 260
XspI CTAG 2 cut(s) 96, 408
Zsp2I ATGCAT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.