AT1G72370

Required for the assembly and or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA- precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004147
Physical Location & Seq
Reverse (-)
27242845 .. 27244931
2087 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G72370.2

Sequence Viewer

Length: 897 bp
ATGGCGACTAATGGATCTGCTAGCTCAGCGCAGCTCTCTCAGAAGGAAGCTGATGTCAGGATGATGTGTGCCGCTGAGGTTCATCTTGGAACCAAGAATTGCAATTACCAGATGGAGCGTTACGTCTTCAAGAGACGCAACGATGGTATTTACATCTTCAACCTTGGCAAAACATGGGAAAAGCTCCAGATGGCTGCTAGAGTTATTGTTGCCATTGAGAACCCACAGGACATCATCGTCCAGTCAGCTAGGCCCTATGGACAGAGAGCTGTGTTGAAGTTTGCTCAGTACACTGGAGCCAATGCCATTGCTGGAAGACACACTCCTGGTACCTTCACCAATCAGATGCAGACATCCTTCAGTGAACCAAGGCTGTTGATTCTTACTGACCCAAGAACTGACCACCAGCCTATCAAGGAAGGTGCTTTGGGAAACATTCCAATCATTGCTTTCTGTGACACAGACTCTCCAATGAGATTTGTTGACATTGGTATCCCAGCCAACAACAAGGGAAAGCACAGCATTGGGTGCCTTTTCTGGCTTCTGGCTCGCATGGTCCTTCAGATGCGTGGAACCATTGCTGCTGGACAGAAGTGGGATGTCATGGTTGACCTGTTCTTTTACAGGGAACCTGAGGAGACTAAGCCAGAGGATGAAGATGAAGCTGGCCCTCAGGCAGAATATGGTGCACTTCCTGCTCCAGAATATGGAATGGTAGGTGGAGACCAATGGACCACCGCTCAGATCCCTGATGCTGCATGGCCAGGAGAAGGTCAGGCACCAATCTCTGCTGCACCTGCTGCTGCTTCTTGGAGTGATTCCGCTGCTGCACCGGCAGATGGTGGATGGGAAGCTGCCGCGCCACCCTCAGGGGCACCAGCTGCTGGTTGGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000028 GO:0000447 GO:0000460 GO:0000461 GO:0000462 GO:0000466 GO:0000469 GO:0000478 GO:0000479 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0005911 GO:0006139 GO:0006364 GO:0006396 GO:0006403 GO:0006405 GO:0006407 GO:0006412 GO:0006518 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006970 GO:0006996 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009987 GO:0010467 GO:0015031 GO:0015833 GO:0015931 GO:0015935 GO:0016020 GO:0016043 GO:0016070 GO:0016072 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0022626 GO:0022627 GO:0030054 GO:0030490 GO:0030684 GO:0030686 GO:0031123 GO:0031125 GO:0031503 GO:0032991 GO:0033036 GO:0034470 GO:0034613 GO:0034622 GO:0034641 GO:0034645 GO:0034660 GO:0042254 GO:0042255 GO:0042274 GO:0042788 GO:0042886 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043603 GO:0043604 GO:0043628 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0050657 GO:0050658 GO:0050896 GO:0051029 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0055044 GO:0065003 GO:0070727 GO:0070925 GO:0071166 GO:0071426 GO:0071428 GO:0071702 GO:0071704 GO:0071705 GO:0071826 GO:0071840 GO:0071944 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097064 GO:0097159 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
40S_SA_C Ribosomal_S2

Protein Analysis

298

Amino Acids

32.29

Weight (kDa)

5.05

Isoelectric Point (pI)

33.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S2 PF00318 23 - 117 2.6e-11 Ribosomal protein S2
Ribosomal_S2 PF00318 120 - 186 1.4e-10 Ribosomal protein S2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 805
AasI GACNNNNNNGTC 1 cut(s) 236
Acc36I ACCTGC 1 cut(s) 805
Acc65I GGTACC 1 cut(s) 329
AccB1I GGYRCC 4 cut(s) 329, 528, 778, 874
AccB7I CCANNNNNTGG 2 cut(s) 707, 884
AccBSI CCGCTC 1 cut(s) 740
AccII CGCG 1 cut(s) 860
AciI CCGC 4 cut(s) 72, 738, 822, 858
AclWI GGATC 2 cut(s) 22, 739
AcoI YGGCCR 1 cut(s) 761
AcuI CTGAAG 2 cut(s) 343, 545
AfaI GTAC 2 cut(s) 290, 331
AfiI CCNNNNNNNGG 5 cut(s) 707, 770, 839, 869, 884
AgsI TTSAA 3 cut(s) 130, 160, 277
AjnI CCWGG 2 cut(s) 325, 763
AluBI AGCT 9 cut(s) 24, 34, 50, 184, 248, 269, 665, 854, 881
AluI AGCT 9 cut(s) 24, 34, 50, 184, 248, 269, 665, 854, 881
Alw21I GWGCWC 1 cut(s) 691
Alw26I GTCTC 3 cut(s) 127, 632, 717
Alw44I GTGCAC 1 cut(s) 687
AlwI GGATC 2 cut(s) 22, 739
AlwNI CAGNNNCTG 1 cut(s) 884
AoxI GGCC 3 cut(s) 251, 667, 761
ApaLI GTGCAC 1 cut(s) 687
Asp718I GGTACC 1 cut(s) 329
AspLEI GCGC 2 cut(s) 31, 862
AspS9I GGNCC 4 cut(s) 252, 556, 668, 732
AsuHPI GGTGA 1 cut(s) 328
AsuNHI GCTAGC 1 cut(s) 20
AvaII GGWCC 2 cut(s) 556, 732
AxyI CCTNAGG 3 cut(s) 633, 672, 868
BaeGI GKGCMC 2 cut(s) 691, 877
BaeI ACNNNNGTAYC 2 cut(s) 475, 508
BalI TGGCCA 1 cut(s) 763
BanI GGYRCC 4 cut(s) 329, 528, 778, 874
BbsI GAAGAC 2 cut(s) 118, 322
Bbv12I GWGCWC 1 cut(s) 691
BbvCI CCTCAGC 1 cut(s) 75
BccI CCATC 5 cut(s) 106, 137, 184, 833, 840
BciT130I CCWGG 2 cut(s) 327, 765
BciVI GTATCC 1 cut(s) 503
BcoDI GTCTC 3 cut(s) 127, 632, 717
BfaI CTAG 3 cut(s) 21, 198, 249
BfuAI ACCTGC 1 cut(s) 805
BfuI GTATCC 1 cut(s) 503
BlpI GCTNAGC 1 cut(s) 25
Bme1390I CCNGG 2 cut(s) 327, 765
Bme18I GGWCC 2 cut(s) 556, 732
BmgT120I GGNCC 4 cut(s) 252, 556, 668, 732
BmiI GGNNCC 8 cut(s) 91, 298, 331, 530, 574, 630, 780, 876
BmrFI CCNGG 2 cut(s) 327, 765
BmsI GCATC 3 cut(s) 336, 555, 742
BmtI GCTAGC 1 cut(s) 24
BpiI GAAGAC 2 cut(s) 118, 322
BpmI CTGGAG 3 cut(s) 170, 315, 684
Bpu10I CCTNAGC 1 cut(s) 75
Bpu1102I GCTNAGC 1 cut(s) 25
BsaI GGTCTC 1 cut(s) 717
BsaJI CCNNGG 2 cut(s) 163, 368
BsaXI ACNNNNNCTCC 2 cut(s) 451, 481
Bsc4I CCNNNNNNNGG 5 cut(s) 707, 770, 839, 869, 884
Bse118I RCCGGY 1 cut(s) 832
Bse1I ACTGG 2 cut(s) 241, 298
Bse21I CCTNAGG 3 cut(s) 633, 672, 868
Bse3DI GCAATG 3 cut(s) 306, 444, 576
BseBI CCWGG 2 cut(s) 327, 765
BseDI CCNNGG 2 cut(s) 163, 368
BseGI GGATG 5 cut(s) 66, 353, 604, 658, 851
BseLI CCNNNNNNNGG 5 cut(s) 707, 770, 839, 869, 884
BseMI GCAATG 3 cut(s) 306, 444, 576
BseMII CTCAG 8 cut(s) 39, 53, 66, 299, 624, 686, 755, 882
BseNI ACTGG 2 cut(s) 241, 298
BseRI GAGGAG 1 cut(s) 650
BseSI GKGCMC 2 cut(s) 691, 877
BseYI CCCAGC 1 cut(s) 496
BsgI GTGCAG 2 cut(s) 777, 813
Bsh1236I CGCG 1 cut(s) 860
BshFI GGCC 3 cut(s) 253, 669, 763
BshNI GGYRCC 4 cut(s) 329, 528, 778, 874
BsiHKAI GWGCWC 1 cut(s) 691
BsiSI CCGG 1 cut(s) 833
BslI CCNNNNNNNGG 5 cut(s) 707, 770, 839, 869, 884
BsmAI GTCTC 3 cut(s) 127, 632, 717
BsmBI CGTCTC 1 cut(s) 127
BsnI GGCC 3 cut(s) 253, 669, 763
Bso31I GGTCTC 1 cut(s) 717
Bsp1286I GDGCHC 2 cut(s) 691, 877
Bsp143I GATC 2 cut(s) 14, 744
Bsp1720I GCTNAGC 1 cut(s) 25
BspACI CCGC 4 cut(s) 72, 738, 822, 858
BspANI GGCC 3 cut(s) 253, 669, 763
BspCNI CTCAG 8 cut(s) 38, 52, 67, 298, 625, 685, 754, 881
BspFNI CGCG 1 cut(s) 860
BspLI GGNNCC 8 cut(s) 91, 298, 331, 530, 574, 630, 780, 876
BspMI ACCTGC 1 cut(s) 805
BspOI GCTAGC 1 cut(s) 24
BspPI GGATC 2 cut(s) 22, 739
BspT107I GGYRCC 4 cut(s) 329, 528, 778, 874
BspTNI GGTCTC 1 cut(s) 717
BsrBI CCGCTC 1 cut(s) 740
BsrDI GCAATG 3 cut(s) 306, 444, 576
BsrFI RCCGGY 1 cut(s) 832
BsrI ACTGG 2 cut(s) 241, 298
BssAI RCCGGY 1 cut(s) 832
BssECI CCNNGG 2 cut(s) 163, 368
BssMI GATC 2 cut(s) 14, 744
BssT1I CCWWGG 2 cut(s) 163, 368
Bst2UI CCWGG 2 cut(s) 327, 765
BstAPI GCANNNNNTGC 4 cut(s) 528, 695, 800, 881
BstC8I GCNNGC 3 cut(s) 22, 550, 667
BstDEI CTNAG 9 cut(s) 25, 39, 75, 285, 633, 642, 672, 741, 868
BstF5I GGATG 5 cut(s) 66, 353, 604, 658, 851
BstFNI CGCG 1 cut(s) 860
BstHHI GCGC 2 cut(s) 31, 862
BstKTI GATC 2 cut(s) 17, 747
BstMAI GTCTC 3 cut(s) 127, 632, 717
BstMBI GATC 2 cut(s) 14, 744
BstMWI GCNNNNNNNGC 7 cut(s) 26, 528, 695, 797, 800, 833, 881
BstNI CCWGG 2 cut(s) 327, 765
BstSCI CCNGG 2 cut(s) 325, 763
BstSLI GKGCMC 2 cut(s) 691, 877
BstUI CGCG 1 cut(s) 860
BstV2I GAAGAC 2 cut(s) 118, 322
BstX2I RGATCY 2 cut(s) 14, 744
BstYI RGATCY 2 cut(s) 14, 744
Bsu36I CCTNAGG 3 cut(s) 633, 672, 868
BsuI GTATCC 1 cut(s) 503
BsuRI GGCC 3 cut(s) 253, 669, 763
BtsCI GGATG 5 cut(s) 66, 353, 604, 658, 851
BtsIMutI CAGTG 2 cut(s) 291, 367
BveI ACCTGC 1 cut(s) 805
Cac8I GCNNGC 3 cut(s) 22, 550, 667
CaiI CAGNNNCTG 1 cut(s) 884
CfoI GCGC 2 cut(s) 31, 862
Cfr10I RCCGGY 1 cut(s) 832
Cfr13I GGNCC 4 cut(s) 252, 556, 668, 732
CseI GACGC 1 cut(s) 144
Csp6I GTAC 2 cut(s) 289, 330
CviAII CATG 4 cut(s) 174, 553, 604, 759
CviQI GTAC 2 cut(s) 289, 330
DdeI CTNAG 9 cut(s) 25, 39, 75, 285, 633, 642, 672, 741, 868
DpnI GATC 2 cut(s) 16, 746
DpnII GATC 2 cut(s) 14, 744
DrdI GACNNNNNNGTC 1 cut(s) 236
DseDI GACNNNNNNGTC 1 cut(s) 236
EaeI YGGCCR 1 cut(s) 761
Eco130I CCWWGG 2 cut(s) 163, 368
Eco31I GGTCTC 1 cut(s) 717
Eco47I GGWCC 2 cut(s) 556, 732
Eco57I CTGAAG 2 cut(s) 343, 545
Eco81I CCTNAGG 3 cut(s) 633, 672, 868
EcoO109I RGGNCCY 1 cut(s) 252
EcoRII CCWGG 2 cut(s) 325, 763
EcoT14I CCWWGG 2 cut(s) 163, 368
ErhI CCWWGG 2 cut(s) 163, 368
Esp3I CGTCTC 1 cut(s) 127
FaeI CATG 4 cut(s) 177, 556, 607, 762
FaiI YATR 7 cut(s) 175, 258, 554, 605, 684, 708, 760
FatI CATG 4 cut(s) 173, 552, 603, 758
FokI GGATG 5 cut(s) 73, 340, 611, 665, 858
FspBI CTAG 3 cut(s) 21, 198, 249
GlaI GCGC 2 cut(s) 30, 861
GsaI CCCAGC 1 cut(s) 500
GsuI CTGGAG 3 cut(s) 170, 315, 684
HaeIII GGCC 3 cut(s) 253, 669, 763
HapII CCGG 1 cut(s) 833
HgaI GACGC 1 cut(s) 144
HhaI GCGC 2 cut(s) 31, 862
Hin1II CATG 4 cut(s) 177, 556, 607, 762
Hin6I GCGC 2 cut(s) 29, 860
HinP1I GCGC 2 cut(s) 29, 860
HincII GTYRAC 2 cut(s) 484, 610
HindII GTYRAC 2 cut(s) 484, 610
HinfI GANTC 3 cut(s) 379, 464, 818
HpaII CCGG 1 cut(s) 833
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 5 cut(s) 291, 365, 484, 610, 689
Hpy188I TCNGA 4 cut(s) 42, 345, 564, 744
Hpy188III TCNNGA 4 cut(s) 58, 130, 187, 701
Hpy8I GTNNAC 5 cut(s) 291, 365, 484, 610, 689
HpyAV CCTTC 6 cut(s) 37, 343, 367, 413, 569, 764
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 6 cut(s) 102, 349, 689, 758, 794, 830
HpyF10VI GCNNNNNNNGC 7 cut(s) 26, 528, 695, 797, 800, 833, 881
HpyF3I CTNAG 9 cut(s) 25, 39, 75, 285, 633, 642, 672, 741, 868
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 4 cut(s) 177, 556, 607, 762
HspAI GCGC 2 cut(s) 29, 860
KpnI GGTACC 1 cut(s) 333
Kzo9I GATC 2 cut(s) 14, 744
LmnI GCTCC 4 cut(s) 115, 189, 296, 703
LweI GCATC 3 cut(s) 336, 555, 742
MaeI CTAG 3 cut(s) 21, 198, 249
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 2 cut(s) 119, 455
MalI GATC 2 cut(s) 16, 746
MbiI CCGCTC 1 cut(s) 740
MboI GATC 2 cut(s) 14, 744
MboII GAAGA 4 cut(s) 118, 148, 327, 668
MflI RGATCY 2 cut(s) 14, 744
MhlI GDGCHC 2 cut(s) 691, 877
MlsI TGGCCA 1 cut(s) 763
MluCI AATT 2 cut(s) 97, 103
MluNI TGGCCA 1 cut(s) 763
MlyI GAGTC 1 cut(s) 458
MnlI CCTC 5 cut(s) 70, 628, 643, 681, 877
Mox20I TGGCCA 1 cut(s) 763
MscI TGGCCA 1 cut(s) 763
Msp20I TGGCCA 1 cut(s) 763
MspA1I CMGCKG 3 cut(s) 74, 824, 881
MspI CCGG 1 cut(s) 833
MspR9I CCNGG 2 cut(s) 327, 765
MvaI CCWGG 2 cut(s) 327, 765
MvnI CGCG 1 cut(s) 860
MwoI GCNNNNNNNGC 7 cut(s) 26, 528, 695, 797, 800, 833, 881
NdeII GATC 2 cut(s) 14, 744
NheI GCTAGC 1 cut(s) 20
NlaIII CATG 4 cut(s) 177, 556, 607, 762
NlaIV GGNNCC 8 cut(s) 91, 298, 331, 530, 574, 630, 780, 876
NmuCI GTSAC 1 cut(s) 455
PaqCI CACCTGC 1 cut(s) 805
PfeI GAWTC 2 cut(s) 379, 818
PflMI CCANNNNNTGG 2 cut(s) 707, 884
PleI GAGTC 1 cut(s) 458
PpsI GAGTC 1 cut(s) 458
Psp6I CCWGG 2 cut(s) 325, 763
PspFI CCCAGC 1 cut(s) 496
PspGI CCWGG 2 cut(s) 325, 763
PspN4I GGNNCC 8 cut(s) 91, 298, 331, 530, 574, 630, 780, 876
PspPI GGNCC 4 cut(s) 252, 556, 668, 732
PstNI CAGNNNCTG 1 cut(s) 884
PsuI RGATCY 2 cut(s) 14, 744
PvuII CAGCTG 1 cut(s) 881
RsaI GTAC 2 cut(s) 290, 331
RsaNI GTAC 2 cut(s) 289, 330
Sau3AI GATC 2 cut(s) 14, 744
Sau96I GGNCC 4 cut(s) 252, 556, 668, 732
SchI GAGTC 1 cut(s) 458
ScrFI CCNGG 2 cut(s) 327, 765
SduI GDGCHC 2 cut(s) 691, 877
SfaNI GCATC 3 cut(s) 336, 555, 742
SinI GGWCC 2 cut(s) 556, 732
Sse9I AATT 2 cut(s) 97, 103
SsiI CCGC 4 cut(s) 72, 738, 822, 858
SspMI CTAG 3 cut(s) 21, 198, 249
StyD4I CCNGG 2 cut(s) 325, 763
StyI CCWWGG 2 cut(s) 163, 368
TaiI ACGT 1 cut(s) 126
TasI AATT 2 cut(s) 97, 103
TatI WGTACW 1 cut(s) 288
TauI GCSGC 2 cut(s) 74, 860
TfiI GAWTC 2 cut(s) 379, 818
TscAI CASTG 2 cut(s) 298, 367
TseFI GTSAC 1 cut(s) 455
Tsp45I GTSAC 1 cut(s) 455
TspDTI ATGAA 3 cut(s) 71, 669, 675
TspRI CASTG 2 cut(s) 298, 367
Van91I CCANNNNNTGG 2 cut(s) 707, 884
VneI GTGCAC 1 cut(s) 687
VpaK11BI GGWCC 2 cut(s) 556, 732
XcmI CCANNNNNNNNNTGG 1 cut(s) 885
XspI CTAG 3 cut(s) 21, 198, 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.