Rorug07G0226500

Required for the assembly and or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA- precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0004147
Physical Location & Seq
Forward (+)
20667543 .. 20670743
3201 bp
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UTR
Exon/CDS
Intron
Rorug07G0226500.1

Sequence Viewer

Length: 735 bp
ATGGGGCATCCTACAAAAGCAACAGGACTAGCCTCCAACTCTCCATCCCAAAAAAGTAAAAGCACCAACATGACAGTAGACAGCGCCACCATGGAAGACATGGAAGCCAAGGCCGAGCAGCTCGGTATCCAATTCTCCTCCATAGACTTGAACTCTGCACACCCACCTCAATTTAATTTCAGTGAATGCCAAGAAGTGTTTTACGATGACAATGGTTTCATCCCCAATATTGAGTTTGGGGGTGTGATCGTGGTCAATAACCTTCCCATTGTTGAGGCAGAGATGGCCCAGGAGCTTGAAAACGAGATTCGAGTGGTGTTTAGTGAAGTTGGTGTTATTAAAGAAGATGGGTTCTCGATGCCTGTGAATCCCAGCACCCAAGAAACCTTGGGTCATTGTTTCATAGAGTTTAATTCTCGTCAGGAAGCCACACTTGCTAGGAGCAAGAGAGATGGATACAAGTTTGGTAACTGCAAGTTGTCAGTAAGCCTCTATGATGCCTTTGGCAGTGAAGTTGCTACCCCTGACATGGGGACTTATTTAGAGACTAATGCATCCTTTTGTGGGTCCTCAAGGTATCCAGGGTCGCCTTCCTTGCTTAGTCCTGTAACAGAGGAAGATGAAGAACCCGTTCATAGTGATGAAACTAGTGCAGATGACCACGATGACATAATGCAGTGCAGTGAGCAAGACAATTATATGAGCAGTGAGGAATTTAGGGAAGTCATTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000028 GO:0000447 GO:0000460 GO:0000461 GO:0000462 GO:0000466 GO:0000469 GO:0000478 GO:0000479 GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0006139 GO:0006364 GO:0006396 GO:0006403 GO:0006405 GO:0006407 GO:0006412 GO:0006518 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0015031 GO:0015833 GO:0015931 GO:0015935 GO:0016043 GO:0016070 GO:0016072 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0022626 GO:0022627 GO:0030490 GO:0030684 GO:0030686 GO:0031123 GO:0031125 GO:0031503 GO:0032991 GO:0033036 GO:0034470 GO:0034613 GO:0034622 GO:0034641 GO:0034645 GO:0034660 GO:0042254 GO:0042255 GO:0042274 GO:0042886 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0043628 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0050657 GO:0050658 GO:0051029 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0065003 GO:0070727 GO:0070925 GO:0071166 GO:0071426 GO:0071428 GO:0071702 GO:0071704 GO:0071705 GO:0071826 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097064 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
40S_SA_C Ribosomal_S2

Protein Analysis

244

Amino Acids

26.88

Weight (kDa)

4.21

Isoelectric Point (pI)

58.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 100 - 160 3.3e-07 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 78
AcsI RAATTY 1 cut(s) 713
AfiI CCNNNNNNNGG 3 cut(s) 529, 530, 564
AgsI TTSAA 3 cut(s) 151, 299, 731
AhlI ACTAGT 1 cut(s) 647
AjnI CCWGG 2 cut(s) 288, 580
AluBI AGCT 2 cut(s) 121, 295
AluI AGCT 2 cut(s) 121, 295
Alw26I GTCTC 1 cut(s) 539
AoxI GGCC 2 cut(s) 111, 285
ApeKI GCWGC 1 cut(s) 118
ApoI RAATTY 1 cut(s) 713
Asp700I GAANNNNTTC 1 cut(s) 630
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 2 cut(s) 286, 567
AvaII GGWCC 1 cut(s) 567
BbsI GAAGAC 1 cut(s) 102
BbvI GCAGC 1 cut(s) 130
BccI CCATC 4 cut(s) 52, 277, 341, 446
BciT130I CCWGG 2 cut(s) 290, 582
BciVI GTATCC 3 cut(s) 137, 449, 588
BcoDI GTCTC 1 cut(s) 539
BcuI ACTAGT 1 cut(s) 647
BfaI CTAG 3 cut(s) 29, 438, 648
BfoI RGCGCY 1 cut(s) 87
BfuI GTATCC 3 cut(s) 137, 449, 588
BisI GCNGC 1 cut(s) 119
BlsI GCNGC 1 cut(s) 120
Bme1390I CCNGG 2 cut(s) 290, 582
Bme18I GGWCC 1 cut(s) 567
BmgT120I GGNCC 2 cut(s) 286, 567
BmiI GGNNCC 1 cut(s) 568
BmrFI CCNGG 2 cut(s) 290, 582
BmsI GCATC 4 cut(s) 16, 348, 487, 563
BpiI GAAGAC 1 cut(s) 102
BpuEI CTTGAG 1 cut(s) 556
BsaJI CCNNGG 5 cut(s) 90, 108, 288, 387, 581
Bsc4I CCNNNNNNNGG 3 cut(s) 529, 530, 564
BseBI CCWGG 2 cut(s) 290, 582
BseDI CCNNGG 5 cut(s) 90, 108, 288, 387, 581
BseGI GGATG 4 cut(s) 7, 44, 219, 554
BseLI CCNNNNNNNGG 3 cut(s) 529, 530, 564
BseRI GAGGAG 1 cut(s) 127
BseXI GCAGC 1 cut(s) 130
BseYI CCCAGC 1 cut(s) 371
BsgI GTGCAG 3 cut(s) 141, 672, 700
BshFI GGCC 2 cut(s) 113, 287
BslFI GGGAC 1 cut(s) 547
BslI CCNNNNNNNGG 3 cut(s) 529, 530, 564
BsmAI GTCTC 1 cut(s) 539
BsmFI GGGAC 1 cut(s) 547
BsmI GAATGC 1 cut(s) 191
BsnI GGCC 2 cut(s) 113, 287
Bsp143I GATC 1 cut(s) 246
Bsp19I CCATGG 1 cut(s) 90
BspANI GGCC 2 cut(s) 113, 287
BspLI GGNNCC 1 cut(s) 568
BssECI CCNNGG 5 cut(s) 90, 108, 288, 387, 581
BssMI GATC 1 cut(s) 246
BssT1I CCWWGG 3 cut(s) 90, 108, 387
Bst2UI CCWGG 2 cut(s) 290, 582
Bst4CI ACNGT 1 cut(s) 76
BstDEI CTNAG 1 cut(s) 599
BstDSI CCRYGG 1 cut(s) 90
BstF5I GGATG 4 cut(s) 7, 44, 219, 554
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 1 cut(s) 249
BstMAI GTCTC 1 cut(s) 539
BstMBI GATC 1 cut(s) 246
BstMWI GCNNNNNNNGC 3 cut(s) 284, 434, 595
BstNI CCWGG 2 cut(s) 290, 582
BstSCI CCNGG 2 cut(s) 288, 580
BstV1I GCAGC 1 cut(s) 130
BstV2I GAAGAC 1 cut(s) 102
BsuI GTATCC 3 cut(s) 137, 449, 588
BsuRI GGCC 2 cut(s) 113, 287
BtgI CCRYGG 1 cut(s) 90
BtsCI GGATG 4 cut(s) 7, 44, 219, 554
BtsI GCAGTG 4 cut(s) 514, 683, 688, 712
BtsIMutI CAGTG 5 cut(s) 187, 514, 683, 688, 712
CfoI GCGC 1 cut(s) 86
Cfr13I GGNCC 2 cut(s) 286, 567
CviAII CATG 4 cut(s) 70, 91, 100, 529
CviJI RGCY 8 cut(s) 32, 107, 113, 121, 287, 295, 428, 489
CviKI_1 RGCY 8 cut(s) 32, 107, 113, 121, 287, 295, 428, 489
DdeI CTNAG 1 cut(s) 599
DpnI GATC 1 cut(s) 248
DpnII GATC 1 cut(s) 246
Eco130I CCWWGG 3 cut(s) 90, 108, 387
Eco47I GGWCC 1 cut(s) 567
EcoO109I RGGNCCY 1 cut(s) 567
EcoRII CCWGG 2 cut(s) 288, 580
EcoT14I CCWWGG 3 cut(s) 90, 108, 387
EcoT22I ATGCAT 1 cut(s) 556
ErhI CCWWGG 3 cut(s) 90, 108, 387
FaeI CATG 4 cut(s) 73, 94, 103, 532
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 1 cut(s) 547
FatI CATG 4 cut(s) 69, 90, 99, 528
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 1 cut(s) 119
FokI GGATG 3 cut(s) 31, 206, 541
Fsp4HI GCNGC 1 cut(s) 119
FspBI CTAG 3 cut(s) 29, 438, 648
GlaI GCGC 1 cut(s) 85
GluI GCNGC 1 cut(s) 119
GsaI CCCAGC 1 cut(s) 375
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 2 cut(s) 113, 287
HhaI GCGC 1 cut(s) 86
Hin1II CATG 4 cut(s) 73, 94, 103, 532
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HinfI GANTC 2 cut(s) 307, 367
Hpy166II GTNNAC 1 cut(s) 79
Hpy188III TCNNGA 2 cut(s) 355, 422
Hpy8I GTNNAC 1 cut(s) 79
HpyAV CCTTC 2 cut(s) 272, 600
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4V TGCA 6 cut(s) 158, 474, 554, 653, 676, 681
HpyF10VI GCNNNNNNNGC 3 cut(s) 284, 434, 595
HpyF3I CTNAG 1 cut(s) 599
Hsp92II CATG 4 cut(s) 73, 94, 103, 532
HspAI GCGC 1 cut(s) 84
Kzo9I GATC 1 cut(s) 246
LmnI GCTCC 2 cut(s) 292, 441
Lsp1109I GCAGC 1 cut(s) 130
LweI GCATC 4 cut(s) 16, 348, 487, 563
MaeI CTAG 3 cut(s) 29, 438, 648
MaeIII GTNAC 2 cut(s) 467, 607
MalI GATC 1 cut(s) 248
MboI GATC 1 cut(s) 246
MboII GAAGA 4 cut(s) 107, 356, 629, 635
MluCI AATT 6 cut(s) 131, 170, 175, 412, 694, 713
MmeI TCCRAC 1 cut(s) 60
MnlI CCTC 8 cut(s) 43, 148, 177, 268, 500, 580, 607, 703
Mph1103I ATGCAT 1 cut(s) 556
MroXI GAANNNNTTC 1 cut(s) 630
MseI TTAA 3 cut(s) 174, 339, 411
MslI CAYNNNNRTG 2 cut(s) 68, 639
MspR9I CCNGG 2 cut(s) 290, 582
Mva1269I GAATGC 1 cut(s) 191
MvaI CCWGG 2 cut(s) 290, 582
MwoI GCNNNNNNNGC 3 cut(s) 284, 434, 595
NcoI CCATGG 1 cut(s) 90
NdeII GATC 1 cut(s) 246
NlaIII CATG 4 cut(s) 73, 94, 103, 532
NlaIV GGNNCC 1 cut(s) 568
NmeAIII GCCGAG 1 cut(s) 139
NsiI ATGCAT 1 cut(s) 556
PctI GAATGC 1 cut(s) 191
PdmI GAANNNNTTC 1 cut(s) 630
PfeI GAWTC 2 cut(s) 307, 367
PkrI GCNGC 1 cut(s) 120
PpuMI RGGWCCY 1 cut(s) 567
Psp5II RGGWCCY 1 cut(s) 567
Psp6I CCWGG 2 cut(s) 288, 580
PspFI CCCAGC 1 cut(s) 371
PspGI CCWGG 2 cut(s) 288, 580
PspN4I GGNNCC 1 cut(s) 568
PspPI GGNCC 2 cut(s) 286, 567
PspPPI RGGWCCY 1 cut(s) 567
RseI CAYNNNNRTG 2 cut(s) 68, 639
SaqAI TTAA 3 cut(s) 174, 339, 411
SatI GCNGC 1 cut(s) 119
Sau3AI GATC 1 cut(s) 246
Sau96I GGNCC 2 cut(s) 286, 567
ScrFI CCNGG 2 cut(s) 290, 582
SetI ASST 6 cut(s) 123, 169, 264, 297, 389, 578
SfaNI GCATC 4 cut(s) 16, 348, 487, 563
SinI GGWCC 1 cut(s) 567
SmiMI CAYNNNNRTG 2 cut(s) 68, 639
SmlI CTYRAG 1 cut(s) 571
SmoI CTYRAG 1 cut(s) 571
SpeI ACTAGT 1 cut(s) 647
Sse9I AATT 6 cut(s) 131, 170, 175, 412, 694, 713
SspI AATATT 1 cut(s) 229
SspMI CTAG 3 cut(s) 29, 438, 648
StyD4I CCNGG 2 cut(s) 288, 580
StyI CCWWGG 3 cut(s) 90, 108, 387
TaaI ACNGT 1 cut(s) 76
TaqI TCGA 2 cut(s) 310, 356
TasI AATT 6 cut(s) 131, 170, 175, 412, 694, 713
TfiI GAWTC 2 cut(s) 307, 367
Tru1I TTAA 3 cut(s) 174, 339, 411
Tru9I TTAA 3 cut(s) 174, 339, 411
TscAI CASTG 5 cut(s) 187, 514, 683, 688, 712
TseI GCWGC 1 cut(s) 118
TspDTI ATGAA 5 cut(s) 208, 391, 623, 636, 657
TspRI CASTG 5 cut(s) 187, 514, 683, 688, 712
VpaK11BI GGWCC 1 cut(s) 567
XapI RAATTY 1 cut(s) 713
XcmI CCANNNNNNNNNTGG 1 cut(s) 97
XmiI GTMKAC 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 630
XspI CTAG 3 cut(s) 29, 438, 648
Zsp2I ATGCAT 1 cut(s) 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.