AT1G74390
ERF Family

Polynucleotidyl transferase, ribonuclease H-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004362
Physical Location & Seq
Reverse (-)
27961537 .. 27963954
2418 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74390.1

Sequence Viewer

Length: 1521 bp
ATGGCTTCGAGTCTGGGCGGCGATGAGAGAAGCGAGATAGCGTTTTTCGACCTTGAGACGGCGGTTCCGACCAAATCAGGGGAGCCTTTCGCGATTTTGGAGTTTGGGGCCATCTTAGTTTGCCCAAGGAGGCTAGAGGAGCTCTATAGTTACTCCACTTTGGTTCGACCTACTGATCTTTCCTTAATCTCTACGCTCACAAAGCGACGTAGCGGCATTACGCGCGACGGAGTTCTCTCTGCACATACATTCTCTGAAATCGCCGATAAGGTCTATGACATTCTTCACGGACGAATTTGGGCGGGACATAACATAATTCGATTCGATTGTGTAAGAATAAGAGAAGCATTTGCAGAAATTGGTCTCTCTCCGCCAGAGCCGAAAGCTACAATTGATTCGCTTTCATTGTTGTCTCAGAAGTTTGGGAAGAGAGCTGGTGACATGAAGATGGCATCACTTGCTACATACTTCGGGCTAGGAGATCAAGCTCACAGGAGCTTAGATGATGTCCGGATGAATCTTGAAGTTGTCAAGTACTGTGCAACCGTCTTGTTTCTGGAGTCCAGTGTTCCTGACATTCTTACAGACATGAGCTGGTTTTCCCCACGAAAAAGTCCCAGAACACGAAGTAATGGGAAGTTAGTGGCTAATGGAGTCAGAGAAAGCTCGACTTCTTCCTCCTCGAGTCCTAAAACTGATCCGAGTTCCTCTTCCGTGGACGCCACAATCGTCAAAAACCATCCCATCGTTTCTCTTCTTACGGAATGCTCAGAAAGTGATGCATCAAGTTATGATATAGAAGATCCAATTGATATAACCACTTTAATAGGTAAACTACGTATTGGAACTCTTCAGACGGATGCTGCAGAAGAAGCGAAAACTGTAAGACAGCAGGATGAATCACCTCCATCACCCGATTCCGATGCCAAAGATGAATCATTTTTGGGTGTTAATGAAGTATCTGTTTCTAGCATCAGGGCAAGTCTTGTCCCGTTTTATCGTGGGAGCTTGAGAATGAAGCTATTTCACAACGACACCCCTCTGCATCTGTGTTGGCATAGCTTGAAAGTTCGGTTCGGAATAAGCCGGAAGTTTGTGGATCATGCAGGTCGTCCAAAGTTGAATATTATTGTTGACGCCCCTCTTGATCTATGCAAGATCTTGGACGCAGTTGATGCTGCTGCGCACAACTTACCAACTGACTCAAGCACAAACTCAGATTGGAGACCCACTGTTATAAGGAAAGAAGGCTTTGCCAACTATCCCACAGCCAGACTGCATATAAGCTCAGAATCCAACGGAGATGATACCCTGTGTGGAACGCAGGTATATCAAAAAGAAGAACCTTTGGGAACCAATCAAAAGCTCGATGTCAGTAGCGATAACTTGGAAAAGCTTGAGTCAGCATTACTTCCCGGTACTCTGGTTGATGCATTCTTCTCACTCGAGCCTTACAGTTATCAGCAAATGGCAGGGATACGTCTAGCAGTCAAAAAGTTGGTGATCCTCCTGAAAAAGTGA

Protein Analysis

506

Amino Acids

55.63

Weight (kDa)

5.75

Isoelectric Point (pI)

50.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 15 - 176 1.3e-24 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1238
Acc16I TGCGCA 1 cut(s) 1185
Acc36I ACCTGC 2 cut(s) 1097, 1315
AccII CGCG 3 cut(s) 92, 223, 225
AccIII TCCGGA 1 cut(s) 510
AciI CCGC 5 cut(s) 18, 62, 213, 302, 371
AclWI GGATC 4 cut(s) 692, 797, 1107, 1498
AcsI RAATTY 1 cut(s) 294
AcuI CTGAAG 1 cut(s) 836
AcyI GRCGYC 2 cut(s) 720, 1137
AfaI GTAC 2 cut(s) 536, 1420
AfiI CCNNNNNNNGG 2 cut(s) 58, 78
AgsI TTSAA 3 cut(s) 524, 1066, 1123
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 4 cut(s) 50, 368, 417, 1219
AlwI GGATC 4 cut(s) 692, 797, 1107, 1498
Ama87I CYCGRG 2 cut(s) 682, 1445
Aor13HI TCCGGA 1 cut(s) 510
AoxI GGCC 1 cut(s) 108
ApeKI GCWGC 3 cut(s) 863, 1178, 1181
ApoI RAATTY 1 cut(s) 294
AspLEI GCGC 2 cut(s) 225, 1186
AspS9I GGNCC 1 cut(s) 108
AsuC2I CCSGG 1 cut(s) 1416
AsuHPI GGTGA 4 cut(s) 449, 894, 903, 1513
AvaI CYCGRG 2 cut(s) 682, 1445
BaeI ACNNNNGTAYC 2 cut(s) 1410, 1443
BanII GRGCYC 1 cut(s) 144
Bbv12I GWGCWC 1 cut(s) 144
BbvI GCAGC 3 cut(s) 850, 1165, 1168
BccI CCATC 5 cut(s) 119, 442, 747, 752, 916
BceAI ACGGC 1 cut(s) 75
BcgI CGANNNNNNTGC 2 cut(s) 905, 939
BciVI GTATCC 1 cut(s) 1470
BcnI CCSGG 1 cut(s) 1416
BcoDI GTCTC 4 cut(s) 50, 368, 417, 1219
BfaI CTAG 4 cut(s) 134, 476, 969, 1484
BfmI CTRYAG 2 cut(s) 145, 864
BfuAI ACCTGC 2 cut(s) 1097, 1315
BfuI GTATCC 1 cut(s) 1470
BglII AGATCT 1 cut(s) 1158
BisI GCNGC 5 cut(s) 19, 214, 864, 1179, 1182
BlsI GCNGC 5 cut(s) 20, 215, 865, 1180, 1183
BmcAI AGTACT 1 cut(s) 536
Bme1390I CCNGG 1 cut(s) 1416
BmeT110I CYCGRG 2 cut(s) 682, 1445
BmgT120I GGNCC 1 cut(s) 108
BmiI GGNNCC 4 cut(s) 66, 84, 109, 1354
BmrFI CCNGG 1 cut(s) 1416
BmsI GCATC 9 cut(s) 461, 769, 791, 850, 913, 981, 1054, 1165, 1420
BpmI CTGGAG 1 cut(s) 578
BpuEI CTTGAG 4 cut(s) 74, 1030, 1189, 1418
BpuMI CCSGG 1 cut(s) 1416
BsaAI YACGTR 1 cut(s) 839
BsaHI GRCGYC 2 cut(s) 720, 1137
BsaI GGTCTC 2 cut(s) 368, 1219
BsaJI CCNNGG 2 cut(s) 125, 714
BsaWI WCCGGW 1 cut(s) 510
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 78
Bse1I ACTGG 1 cut(s) 564
BseAI TCCGGA 1 cut(s) 510
BseDI CCNNGG 2 cut(s) 125, 714
BseGI GGATG 4 cut(s) 519, 739, 865, 901
BseLI CCNNNNNNNGG 2 cut(s) 58, 78
BseMII CTCAG 4 cut(s) 428, 783, 1230, 1302
BseNI ACTGG 1 cut(s) 564
BseRI GAGGAG 2 cut(s) 152, 670
BseXI GCAGC 3 cut(s) 850, 1165, 1168
BsgI GTGCAG 1 cut(s) 225
Bsh1236I CGCG 3 cut(s) 92, 223, 225
BshFI GGCC 1 cut(s) 110
BsiHKAI GWGCWC 1 cut(s) 144
BsiHKCI CYCGRG 2 cut(s) 682, 1445
BsiSI CCGG 3 cut(s) 511, 1087, 1416
BslFI GGGAC 3 cut(s) 318, 600, 974
BslI CCNNNNNNNGG 2 cut(s) 58, 78
BsmAI GTCTC 4 cut(s) 50, 368, 417, 1219
BsmBI CGTCTC 1 cut(s) 50
BsmFI GGGAC 3 cut(s) 318, 600, 974
BsmI GAATGC 2 cut(s) 770, 1433
BsnI GGCC 1 cut(s) 110
Bso31I GGTCTC 2 cut(s) 368, 1219
BsoBI CYCGRG 2 cut(s) 682, 1445
Bsp1286I GDGCHC 1 cut(s) 144
Bsp13I TCCGGA 1 cut(s) 510
Bsp143I GATC 8 cut(s) 175, 481, 697, 802, 1099, 1147, 1158, 1503
Bsp68I TCGCGA 1 cut(s) 92
BspACI CCGC 5 cut(s) 18, 62, 213, 302, 371
BspANI GGCC 1 cut(s) 110
BspCNI CTCAG 4 cut(s) 427, 782, 1229, 1301
BspEI TCCGGA 1 cut(s) 510
BspFNI CGCG 3 cut(s) 92, 223, 225
BspLI GGNNCC 4 cut(s) 66, 84, 109, 1354
BspMAI CTGCAG 1 cut(s) 868
BspMI ACCTGC 2 cut(s) 1097, 1315
BspPI GGATC 4 cut(s) 692, 797, 1107, 1498
BspTNI GGTCTC 2 cut(s) 368, 1219
BsrI ACTGG 1 cut(s) 564
BssECI CCNNGG 2 cut(s) 125, 714
BssMI GATC 8 cut(s) 175, 481, 697, 802, 1099, 1147, 1158, 1503
BssNI GRCGYC 2 cut(s) 720, 1137
BssT1I CCWWGG 1 cut(s) 125
Bst4CI ACNGT 5 cut(s) 539, 547, 883, 1234, 1457
Bst6I CTCTTC 4 cut(s) 422, 715, 759, 855
BstACI GRCGYC 2 cut(s) 720, 1137
BstAPI GCANNNNNTGC 2 cut(s) 458, 1175
BstBAI YACGTR 1 cut(s) 839
BstDEI CTNAG 6 cut(s) 115, 414, 499, 769, 1216, 1288
BstDSI CCRYGG 1 cut(s) 714
BstF5I GGATG 4 cut(s) 519, 739, 865, 901
BstFNI CGCG 3 cut(s) 92, 223, 225
BstHHI GCGC 2 cut(s) 225, 1186
BstKTI GATC 8 cut(s) 178, 484, 700, 805, 1102, 1150, 1161, 1506
BstMAI GTCTC 4 cut(s) 50, 368, 417, 1219
BstMBI GATC 8 cut(s) 175, 481, 697, 802, 1099, 1147, 1158, 1503
BstMWI GCNNNNNNNGC 6 cut(s) 139, 202, 222, 458, 872, 1175
BstSCI CCNGG 1 cut(s) 1414
BstSFI CTRYAG 2 cut(s) 145, 864
BstSNI TACGTA 1 cut(s) 839
BstUI CGCG 3 cut(s) 92, 223, 225
BstV1I GCAGC 3 cut(s) 850, 1165, 1168
BstX2I RGATCY 2 cut(s) 802, 1158
BstYI RGATCY 2 cut(s) 802, 1158
BsuI GTATCC 1 cut(s) 1470
BsuRI GGCC 1 cut(s) 110
BtgI CCRYGG 1 cut(s) 714
BtgZI GCGATG 1 cut(s) 36
BtsCI GGATG 4 cut(s) 519, 739, 865, 901
BtsIMutI CAGTG 2 cut(s) 571, 1230
BtuMI TCGCGA 1 cut(s) 92
BveI ACCTGC 2 cut(s) 1097, 1315
CfoI GCGC 2 cut(s) 225, 1186
Cfr13I GGNCC 1 cut(s) 108
CseI GACGC 3 cut(s) 728, 1145, 1175
Csp6I GTAC 2 cut(s) 535, 1419
CviAII CATG 3 cut(s) 442, 589, 1103
CviQI GTAC 2 cut(s) 535, 1419
DdeI CTNAG 6 cut(s) 115, 414, 499, 769, 1216, 1288
DpnI GATC 8 cut(s) 177, 483, 699, 804, 1101, 1149, 1160, 1505
DpnII GATC 8 cut(s) 175, 481, 697, 802, 1099, 1147, 1158, 1503
Eam1104I CTCTTC 4 cut(s) 422, 715, 759, 855
EarI CTCTTC 4 cut(s) 422, 715, 759, 855
EciI GGCGGA 1 cut(s) 360
Ecl136II GAGCTC 1 cut(s) 142
Eco105I TACGTA 1 cut(s) 839
Eco130I CCWWGG 1 cut(s) 125
Eco24I GRGCYC 1 cut(s) 144
Eco31I GGTCTC 2 cut(s) 368, 1219
Eco53kI GAGCTC 1 cut(s) 142
Eco57I CTGAAG 1 cut(s) 836
Eco88I CYCGRG 2 cut(s) 682, 1445
EcoICRI GAGCTC 1 cut(s) 142
EcoT14I CCWWGG 1 cut(s) 125
EcoT22I ATGCAT 2 cut(s) 784, 1435
EcoT38I GRGCYC 1 cut(s) 144
ErhI CCWWGG 1 cut(s) 125
Esp3I CGTCTC 1 cut(s) 50
FaeI CATG 3 cut(s) 445, 592, 1106
FalI AAGNNNNNCTT 2 cut(s) 655, 687
FaqI GGGAC 3 cut(s) 318, 600, 974
FatI CATG 3 cut(s) 441, 588, 1102
FauI CCCGC 1 cut(s) 295
Fnu4HI GCNGC 5 cut(s) 19, 214, 864, 1179, 1182
FokI GGATG 4 cut(s) 526, 726, 872, 908
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 5 cut(s) 19, 214, 864, 1179, 1182
FspBI CTAG 4 cut(s) 134, 476, 969, 1484
FspI TGCGCA 1 cut(s) 1185
GlaI GCGC 2 cut(s) 224, 1185
GluI GCNGC 5 cut(s) 19, 214, 864, 1179, 1182
GsuI CTGGAG 1 cut(s) 578
HaeIII GGCC 1 cut(s) 110
HapII CCGG 3 cut(s) 511, 1087, 1416
HgaI GACGC 3 cut(s) 728, 1145, 1175
HhaI GCGC 2 cut(s) 225, 1186
Hin1I GRCGYC 2 cut(s) 720, 1137
Hin1II CATG 3 cut(s) 445, 592, 1106
Hin6I GCGC 2 cut(s) 223, 1184
HinP1I GCGC 2 cut(s) 223, 1184
HincII GTYRAC 1 cut(s) 1135
HindII GTYRAC 1 cut(s) 1135
HindIII AAGCTT 1 cut(s) 1394
HpaII CCGG 3 cut(s) 511, 1087, 1416
HphI GGTGA 4 cut(s) 449, 894, 903, 1513
Hpy166II GTNNAC 3 cut(s) 718, 833, 1135
Hpy188III TCNNGA 7 cut(s) 91, 511, 521, 557, 572, 1145, 1510
Hpy8I GTNNAC 3 cut(s) 718, 833, 1135
Hpy99I CGWCG 2 cut(s) 210, 230
HpyAV CCTTC 1 cut(s) 1241
HpyCH4III ACNGT 5 cut(s) 539, 547, 883, 1234, 1457
HpyCH4IV ACGT 3 cut(s) 208, 838, 1480
HpyF10VI GCNNNNNNNGC 6 cut(s) 139, 202, 222, 458, 872, 1175
HpyF3I CTNAG 6 cut(s) 115, 414, 499, 769, 1216, 1288
HpySE526I ACGT 3 cut(s) 208, 838, 1480
Hsp92I GRCGYC 2 cut(s) 720, 1137
Hsp92II CATG 3 cut(s) 445, 592, 1106
HspAI GCGC 2 cut(s) 223, 1184
Kpn2I TCCGGA 1 cut(s) 510
Kzo9I GATC 8 cut(s) 175, 481, 697, 802, 1099, 1147, 1158, 1503
LmnI GCTCC 4 cut(s) 82, 139, 495, 1005
Lsp1109I GCAGC 3 cut(s) 850, 1165, 1168
LweI GCATC 9 cut(s) 461, 769, 791, 850, 913, 981, 1054, 1165, 1420
MaeI CTAG 4 cut(s) 134, 476, 969, 1484
MaeII ACGT 3 cut(s) 208, 838, 1480
MaeIII GTNAC 2 cut(s) 149, 437
MalI GATC 8 cut(s) 177, 483, 699, 804, 1101, 1149, 1160, 1505
MboI GATC 8 cut(s) 175, 481, 697, 802, 1099, 1147, 1158, 1503
MfeI CAATTG 2 cut(s) 390, 807
MflI RGATCY 2 cut(s) 802, 1158
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 5 cut(s) 294, 315, 357, 390, 807
MlyI GAGTC 6 cut(s) 19, 569, 663, 694, 1196, 1409
MmeI TCCRAC 2 cut(s) 92, 1320
MnlI CCTC 9 cut(s) 123, 130, 688, 691, 718, 915, 1050, 1152, 1517
Mph1103I ATGCAT 2 cut(s) 784, 1435
MroI TCCGGA 1 cut(s) 510
MseI TTAA 3 cut(s) 185, 824, 951
MslI CAYNNNNRTG 1 cut(s) 446
MspI CCGG 3 cut(s) 511, 1087, 1416
MspR9I CCNGG 1 cut(s) 1416
MunI CAATTG 2 cut(s) 390, 807
Mva1269I GAATGC 2 cut(s) 770, 1433
MvnI CGCG 3 cut(s) 92, 223, 225
MwoI GCNNNNNNNGC 6 cut(s) 139, 202, 222, 458, 872, 1175
NciI CCSGG 1 cut(s) 1416
NdeII GATC 8 cut(s) 175, 481, 697, 802, 1099, 1147, 1158, 1503
NlaIII CATG 3 cut(s) 445, 592, 1106
NlaIV GGNNCC 4 cut(s) 66, 84, 109, 1354
NmuCI GTSAC 1 cut(s) 437
NruI TCGCGA 1 cut(s) 92
NsbI TGCGCA 1 cut(s) 1185
NsiI ATGCAT 2 cut(s) 784, 1435
PaeR7I CTCGAG 2 cut(s) 682, 1445
PcsI WCGNNNNNNNCGW 2 cut(s) 65, 726
PctI GAATGC 2 cut(s) 770, 1433
PfeI GAWTC 7 cut(s) 321, 395, 517, 899, 917, 935, 1292
PkrI GCNGC 5 cut(s) 20, 215, 865, 1180, 1183
PleI GAGTC 6 cut(s) 18, 568, 662, 693, 1196, 1408
PpsI GAGTC 6 cut(s) 18, 568, 662, 693, 1196, 1408
Ppu21I YACGTR 1 cut(s) 839
PsiI TTATAA 1 cut(s) 1238
Psp124BI GAGCTC 1 cut(s) 144
PspN4I GGNNCC 4 cut(s) 66, 84, 109, 1354
PspPI GGNCC 1 cut(s) 108
PspXI VCTCGAGB 2 cut(s) 682, 1445
PstI CTGCAG 1 cut(s) 868
PsuI RGATCY 2 cut(s) 802, 1158
RruI TCGCGA 1 cut(s) 92
RsaI GTAC 2 cut(s) 536, 1420
RsaNI GTAC 2 cut(s) 535, 1419
RseI CAYNNNNRTG 1 cut(s) 446
SacI GAGCTC 1 cut(s) 144
SaqAI TTAA 3 cut(s) 185, 824, 951
SatI GCNGC 5 cut(s) 19, 214, 864, 1179, 1182
Sau3AI GATC 8 cut(s) 175, 481, 697, 802, 1099, 1147, 1158, 1503
Sau96I GGNCC 1 cut(s) 108
ScaI AGTACT 1 cut(s) 536
SchI GAGTC 6 cut(s) 19, 569, 663, 694, 1196, 1409
ScrFI CCNGG 1 cut(s) 1416
SduI GDGCHC 1 cut(s) 144
SfaNI GCATC 9 cut(s) 461, 769, 791, 850, 913, 981, 1054, 1165, 1420
SfcI CTRYAG 2 cut(s) 145, 864
Sfr274I CTCGAG 2 cut(s) 682, 1445
SlaI CTCGAG 2 cut(s) 682, 1445
SmiMI CAYNNNNRTG 1 cut(s) 446
SmlI CTYRAG 6 cut(s) 53, 682, 1009, 1204, 1397, 1445
SmoI CTYRAG 6 cut(s) 53, 682, 1009, 1204, 1397, 1445
SnaBI TACGTA 1 cut(s) 839
Sse9I AATT 5 cut(s) 294, 315, 357, 390, 807
SsiI CCGC 5 cut(s) 18, 62, 213, 302, 371
SspI AATATT 1 cut(s) 1126
SspMI CTAG 4 cut(s) 134, 476, 969, 1484
SstI GAGCTC 1 cut(s) 144
StyD4I CCNGG 1 cut(s) 1414
StyI CCWWGG 1 cut(s) 125
TaaI ACNGT 5 cut(s) 539, 547, 883, 1234, 1457
TaiI ACGT 3 cut(s) 211, 841, 1483
TaqI TCGA 9 cut(s) 8, 48, 166, 319, 324, 668, 683, 1368, 1446
TasI AATT 5 cut(s) 294, 315, 357, 390, 807
TatI WGTACW 1 cut(s) 534
TauI GCSGC 2 cut(s) 21, 216
TfiI GAWTC 7 cut(s) 321, 395, 517, 899, 917, 935, 1292
Tru1I TTAA 3 cut(s) 185, 824, 951
Tru9I TTAA 3 cut(s) 185, 824, 951
TscAI CASTG 2 cut(s) 571, 1237
TseFI GTSAC 1 cut(s) 437
TseI GCWGC 3 cut(s) 863, 1178, 1181
Tsp45I GTSAC 1 cut(s) 437
TspDTI ATGAA 7 cut(s) 393, 458, 530, 912, 948, 969, 1031
TspGWI ACGGA 6 cut(s) 243, 303, 703, 776, 872, 1314
TspRI CASTG 2 cut(s) 571, 1237
XapI RAATTY 1 cut(s) 294
XhoI CTCGAG 2 cut(s) 682, 1445
XspI CTAG 4 cut(s) 134, 476, 969, 1484
ZrmI AGTACT 1 cut(s) 536
Zsp2I ATGCAT 2 cut(s) 784, 1435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.