AT5G61390
ERF Family

Polynucleotidyl transferase, ribonuclease H-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004362
Physical Location & Seq
Reverse (-)
24678555 .. 24681078
2524 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G61390.1

Sequence Viewer

Length: 1464 bp
ATGTTAACAATGGTTGGTCTAACTCCAGCTGAGGATCGTTCAGAGATTGCCTTTTTCGACGTCGAGACGACAATTCCGTTCCGAGTTGGTCAGGGGTACGCGATTCTGGAGTTTGGATCGATTCTGGTTTGCCCCAAGAAGCTTGTGGAGCTGAAAAACTACTCTGTTCTGGTTCGTCCTGCTAATCTCAACCTCATCACGCCTCGGTCCGTGAAGTGCAACGGCATCAAACGCGAAGACGTCGAATCAGCTCTTACCTTTGCAGATATCGCCGACACCGTCTACGATATTCTCCATGGAAGGATATGGGCAGGTCATAACATATTGAAGTTCGATTGTCCAAGGATAAGAGAAGCATTTGCTGAGATTGGACGAGATCCACCAGAGCCAAAGGGCACCATTGACTCCTTGGCTTTGCTTACTCAAAGGTTTGGGAGAAGAGCTGGTGATATGAAGATGGCGACTTTGGCTACATACTTTGGACTTGGAAACCAGACGCATAGGAGTCTGGACGATGTAAGGATGAACTTCGAGGTTCTCAAGTATTGTGCCACTGTTTTGTTCTTGGAGTCAAGTCTTCCAGATGAACTTATAGAGAACTCGGTTACTACTACTACTCCTGAAACTAGTTCAAGAAGGCGTAGGACTATAAAAAAATCTCCTCTGCAATCTCCCACTGATCAGCAGACAGGAGAGAACATGACAACTATACCTATTTTATCATTTGTTTCATCCGCAGAAGCTCAAACGGATCCATTTGATATGAGCACTTTGAGGAACGAAATAGCCCCTGAAGTTCTTCAATCAGATGTTCCCATGGAAGAAGAACAAAATCAGCAGTCTGAGACTGTTGCCTCTGAAGGTACCGGTGACCAGGAAGGATTCATGGAGCTAGATAAAATATCTGTTTCAAGTATCAGAGCAACTCATGTCCCGCTATATGACGGGAGCCAAACAATGAAGCTGCAACTCTTCCTTGGGGACAGACCCCTTCAGCTCCATTGTCCTCGTCTGAAAGTACGGTTTGGAATCAATGGGAAATTTATGGATAAAGCTGGAAGACGGAGGTTGAACTTTGTAATTGATCTGTATCCAAGTCTCTGCAACGTACTTCAAGAATGCGACAGTGCTGCACAGACAATATCTGTTGATTCAGGCAGTGGTTCTGACTGGAACCCTCTAGTCATTCCAATGAAAGGATTTCTGAACTGTCCCACTGCAAGGATACATATACCCACAGAGTTAAATGGAGATATAGATCGGTACGCAGCTGAGATACACCAGAAAGAGTTCTCTGGAGCTACTGCTACTCAGAAACTCATTTCTAGCAACCCCAAGGCTGAAGAGATTGAGTCTTTGTTGAATCCGAGAACTGTTCTTGACGCTTTCTTGTCTCTGGAGCCATATGATTATCAGCAGAGAGCCGGAATCCGTCTAGTTGCTAGAAAGCTTGTTATACATTAG

Protein Analysis

487

Amino Acids

54.32

Weight (kDa)

5.4

Isoelectric Point (pI)

53.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 18 - 180 2.2e-25 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 63, 243
Acc36I ACCTGC 1 cut(s) 302
Acc65I GGTACC 1 cut(s) 863
AccB1I GGYRCC 2 cut(s) 395, 863
AccI GTMKAC 1 cut(s) 282
AccII CGCG 2 cut(s) 101, 234
AciI CCGC 2 cut(s) 735, 935
AclWI GGATC 5 cut(s) 42, 124, 371, 746, 759
AcsI RAATTY 1 cut(s) 1040
AcuI CTGAAG 4 cut(s) 813, 879, 977, 1362
AcyI GRCGYC 2 cut(s) 60, 240
AfaI GTAC 5 cut(s) 98, 865, 1020, 1110, 1265
AfiI CCNNNNNNNGG 2 cut(s) 1196, 1221
AgeI ACCGGT 1 cut(s) 866
AgsI TTSAA 7 cut(s) 328, 633, 803, 912, 1072, 1115, 1363
AhlI ACTAGT 1 cut(s) 626
AjnI CCWGG 1 cut(s) 873
Alw21I GWGCWC 1 cut(s) 770
Alw26I GTCTC 4 cut(s) 59, 839, 1103, 1398
AlwI GGATC 5 cut(s) 42, 124, 371, 746, 759
ApeKI GCWGC 3 cut(s) 964, 1130, 1268
ApoI RAATTY 1 cut(s) 1040
AsiGI ACCGGT 1 cut(s) 866
Asp700I GAANNNNTTC 2 cut(s) 798, 1289
Asp718I GGTACC 1 cut(s) 863
AspS9I GGNCC 1 cut(s) 207
AsuHPI GGTGA 2 cut(s) 458, 881
AvaII GGWCC 1 cut(s) 207
BaeGI GKGCMC 1 cut(s) 398
BamHI GGATCC 1 cut(s) 751
BanI GGYRCC 2 cut(s) 395, 863
BbsI GAAGAC 3 cut(s) 243, 569, 1066
Bbv12I GWGCWC 1 cut(s) 770
BbvCI CCTCAGC 1 cut(s) 30
BbvI GCAGC 3 cut(s) 951, 1117, 1280
BccI CCATC 1 cut(s) 451
BceAI ACGGC 1 cut(s) 238
BcgI CGANNNNNNTGC 2 cut(s) 1112, 1146
BciT130I CCWGG 1 cut(s) 875
BciVI GTATCC 2 cut(s) 1101, 1218
BclI TGATCA 1 cut(s) 679
BcoDI GTCTC 4 cut(s) 59, 839, 1103, 1398
BcuI ACTAGT 1 cut(s) 626
BfaI CTAG 6 cut(s) 627, 893, 1181, 1326, 1436, 1443
BfuAI ACCTGC 1 cut(s) 302
BfuI GTATCC 2 cut(s) 1101, 1218
BisI GCNGC 3 cut(s) 965, 1131, 1269
BlsI GCNGC 3 cut(s) 966, 1132, 1270
Bme1390I CCNGG 1 cut(s) 875
Bme18I GGWCC 1 cut(s) 207
BmgT120I GGNCC 1 cut(s) 207
BmiI GGNNCC 6 cut(s) 397, 753, 865, 950, 1175, 1401
BmrFI CCNGG 1 cut(s) 875
BmsI GCATC 1 cut(s) 234
BpiI GAAGAC 3 cut(s) 243, 569, 1066
BpmI CTGGAG 4 cut(s) 9, 128, 1317, 1418
Bpu10I CCTNAGC 1 cut(s) 30
BpuEI CTTGAG 1 cut(s) 524
Bsa29I ATCGAT 1 cut(s) 119
BsaBI GATNNNNATC 2 cut(s) 1089, 1257
BsaHI GRCGYC 2 cut(s) 60, 240
BsaJI CCNNGG 7 cut(s) 203, 295, 341, 408, 816, 976, 1335
BsaWI WCCGGW 1 cut(s) 866
BsaXI ACNNNNNCTCC 2 cut(s) 601, 631
Bsc4I CCNNNNNNNGG 2 cut(s) 1196, 1221
Bse118I RCCGGY 1 cut(s) 866
Bse1I ACTGG 1 cut(s) 1175
Bse8I GATNNNNATC 2 cut(s) 1089, 1257
BseBI CCWGG 1 cut(s) 875
BseCI ATCGAT 1 cut(s) 119
BseDI CCNNGG 7 cut(s) 203, 295, 341, 408, 816, 976, 1335
BseGI GGATG 2 cut(s) 528, 731
BseJI GATNNNNATC 2 cut(s) 1089, 1257
BseLI CCNNNNNNNGG 2 cut(s) 1196, 1221
BseMII CTCAG 5 cut(s) 21, 354, 834, 1263, 1325
BseNI ACTGG 1 cut(s) 1175
BseRI GAGGAG 1 cut(s) 651
BseSI GKGCMC 1 cut(s) 398
BseXI GCAGC 3 cut(s) 951, 1117, 1280
BsgI GTGCAG 1 cut(s) 1116
Bsh1236I CGCG 2 cut(s) 101, 234
BshNI GGYRCC 2 cut(s) 395, 863
BshTI ACCGGT 1 cut(s) 866
BshVI ATCGAT 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 770
BsiSI CCGG 2 cut(s) 867, 1425
BslFI GGGAC 3 cut(s) 917, 995, 1197
BslI CCNNNNNNNGG 2 cut(s) 1196, 1221
BsmAI GTCTC 4 cut(s) 59, 839, 1103, 1398
BsmBI CGTCTC 1 cut(s) 59
BsmFI GGGAC 3 cut(s) 917, 995, 1197
BsmI GAATGC 1 cut(s) 1124
Bsp1286I GDGCHC 2 cut(s) 398, 770
Bsp143I GATC 7 cut(s) 34, 116, 376, 679, 751, 1084, 1258
Bsp19I CCATGG 2 cut(s) 295, 816
BspACI CCGC 2 cut(s) 735, 935
BspCNI CTCAG 5 cut(s) 22, 355, 835, 1264, 1324
BspDI ATCGAT 1 cut(s) 119
BspFNI CGCG 2 cut(s) 101, 234
BspLI GGNNCC 6 cut(s) 397, 753, 865, 950, 1175, 1401
BspMI ACCTGC 1 cut(s) 302
BspPI GGATC 5 cut(s) 42, 124, 371, 746, 759
BspQI GCTCTTC 1 cut(s) 433
BspT107I GGYRCC 2 cut(s) 395, 863
BsrFI RCCGGY 1 cut(s) 866
BsrI ACTGG 1 cut(s) 1175
BssAI RCCGGY 1 cut(s) 866
BssECI CCNNGG 7 cut(s) 203, 295, 341, 408, 816, 976, 1335
BssMI GATC 7 cut(s) 34, 116, 376, 679, 751, 1084, 1258
BssNI GRCGYC 2 cut(s) 60, 240
BssT1I CCWWGG 6 cut(s) 295, 341, 408, 816, 976, 1335
Bst2UI CCWGG 1 cut(s) 875
Bst4CI ACNGT 7 cut(s) 280, 556, 850, 1023, 1127, 1211, 1375
Bst6I CTCTTC 3 cut(s) 433, 977, 1338
BstACI GRCGYC 2 cut(s) 60, 240
BstDEI CTNAG 5 cut(s) 30, 363, 843, 1272, 1311
BstDSI CCRYGG 2 cut(s) 295, 816
BstEII GGTNACC 1 cut(s) 869
BstF5I GGATG 2 cut(s) 528, 731
BstFNI CGCG 2 cut(s) 101, 234
BstKTI GATC 7 cut(s) 37, 119, 379, 682, 754, 1087, 1261
BstMAI GTCTC 4 cut(s) 59, 839, 1103, 1398
BstMBI GATC 7 cut(s) 34, 116, 376, 679, 751, 1084, 1258
BstMWI GCNNNNNNNGC 4 cut(s) 148, 231, 269, 467
BstNI CCWGG 1 cut(s) 875
BstPI GGTNACC 1 cut(s) 869
BstSCI CCNGG 1 cut(s) 873
BstSLI GKGCMC 1 cut(s) 398
BstUI CGCG 2 cut(s) 101, 234
BstV1I GCAGC 3 cut(s) 951, 1117, 1280
BstV2I GAAGAC 3 cut(s) 243, 569, 1066
BstX2I RGATCY 2 cut(s) 376, 751
BstYI RGATCY 2 cut(s) 376, 751
Bsu15I ATCGAT 1 cut(s) 119
BsuI GTATCC 2 cut(s) 1101, 1218
BsuTUI ATCGAT 1 cut(s) 119
BtgI CCRYGG 2 cut(s) 295, 816
BtsCI GGATG 2 cut(s) 528, 731
BtsI GCAGTG 2 cut(s) 1165, 1215
BtsIMutI CAGTG 5 cut(s) 552, 675, 1132, 1165, 1215
BveI ACCTGC 1 cut(s) 302
Cfr10I RCCGGY 1 cut(s) 866
Cfr13I GGNCC 1 cut(s) 207
ClaI ATCGAT 1 cut(s) 119
CpoI CGGWCCG 1 cut(s) 207
CseI GACGC 2 cut(s) 505, 1391
Csp6I GTAC 5 cut(s) 97, 864, 1019, 1109, 1264
CspAI ACCGGT 1 cut(s) 866
CspI CGGWCCG 1 cut(s) 207
CviAII CATG 5 cut(s) 296, 700, 817, 886, 929
CviQI GTAC 5 cut(s) 97, 864, 1019, 1109, 1264
DdeI CTNAG 5 cut(s) 30, 363, 843, 1272, 1311
DpnI GATC 7 cut(s) 36, 118, 378, 681, 753, 1086, 1260
DpnII GATC 7 cut(s) 34, 116, 376, 679, 751, 1084, 1258
Eam1104I CTCTTC 3 cut(s) 433, 977, 1338
EarI CTCTTC 3 cut(s) 433, 977, 1338
Eco130I CCWWGG 6 cut(s) 295, 341, 408, 816, 976, 1335
Eco32I GATATC 1 cut(s) 268
Eco47I GGWCC 1 cut(s) 207
Eco57I CTGAAG 4 cut(s) 813, 879, 977, 1362
Eco91I GGTNACC 1 cut(s) 869
EcoO65I GGTNACC 1 cut(s) 869
EcoRII CCWGG 1 cut(s) 873
EcoRV GATATC 1 cut(s) 268
EcoT14I CCWWGG 6 cut(s) 295, 341, 408, 816, 976, 1335
ErhI CCWWGG 6 cut(s) 295, 341, 408, 816, 976, 1335
Esp3I CGTCTC 1 cut(s) 59
FaeI CATG 5 cut(s) 299, 703, 820, 889, 932
FaqI GGGAC 3 cut(s) 917, 995, 1197
FatI CATG 5 cut(s) 295, 699, 816, 885, 928
FauI CCCGC 1 cut(s) 942
FauNDI CATATG 1 cut(s) 1405
FbaI TGATCA 1 cut(s) 679
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 3 cut(s) 965, 1131, 1269
FokI GGATG 2 cut(s) 535, 718
Fsp4HI GCNGC 3 cut(s) 965, 1131, 1269
FspBI CTAG 6 cut(s) 627, 893, 1181, 1326, 1436, 1443
GluI GCNGC 3 cut(s) 965, 1131, 1269
GsuI CTGGAG 4 cut(s) 9, 128, 1317, 1418
HapII CCGG 2 cut(s) 867, 1425
HgaI GACGC 2 cut(s) 505, 1391
Hin1I GRCGYC 2 cut(s) 60, 240
Hin1II CATG 5 cut(s) 299, 703, 820, 889, 932
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 2 cut(s) 140, 1448
HpaI GTTAAC 1 cut(s) 6
HpaII CCGG 2 cut(s) 867, 1425
HphI GGTGA 2 cut(s) 458, 881
Hpy166II GTNNAC 2 cut(s) 6, 283
Hpy8I GTNNAC 2 cut(s) 6, 283
Hpy99I CGWCG 3 cut(s) 62, 65, 245
HpyAV CCTTC 5 cut(s) 294, 630, 854, 872, 1001
HpyCH4III ACNGT 7 cut(s) 280, 556, 850, 1023, 1127, 1211, 1375
HpyCH4IV ACGT 3 cut(s) 60, 240, 1107
HpyCH4V TGCA 7 cut(s) 219, 263, 667, 967, 1104, 1133, 1220
HpyF10VI GCNNNNNNNGC 4 cut(s) 148, 231, 269, 467
HpyF3I CTNAG 5 cut(s) 30, 363, 843, 1272, 1311
HpySE526I ACGT 3 cut(s) 60, 240, 1107
Hsp92I GRCGYC 2 cut(s) 60, 240
Hsp92II CATG 5 cut(s) 299, 703, 820, 889, 932
KpnI GGTACC 1 cut(s) 867
Ksp22I TGATCA 1 cut(s) 679
KspAI GTTAAC 1 cut(s) 6
Kzo9I GATC 7 cut(s) 34, 116, 376, 679, 751, 1084, 1258
LguI GCTCTTC 1 cut(s) 433
LmnI GCTCC 6 cut(s) 148, 889, 948, 1002, 1298, 1399
Lsp1109I GCAGC 3 cut(s) 951, 1117, 1280
LweI GCATC 1 cut(s) 234
MaeI CTAG 6 cut(s) 627, 893, 1181, 1326, 1436, 1443
MaeII ACGT 3 cut(s) 60, 240, 1107
MaeIII GTNAC 2 cut(s) 604, 869
MalI GATC 7 cut(s) 36, 118, 378, 681, 753, 1086, 1260
MboI GATC 7 cut(s) 34, 116, 376, 679, 751, 1084, 1258
MflI RGATCY 2 cut(s) 376, 751
MhlI GDGCHC 2 cut(s) 398, 770
MluCI AATT 3 cut(s) 72, 1040, 1080
MlyI GAGTC 4 cut(s) 398, 514, 578, 1361
MroXI GAANNNNTTC 2 cut(s) 798, 1289
MseI TTAA 2 cut(s) 5, 1244
MslI CAYNNNNRTG 1 cut(s) 1190
MspA1I CMGCKG 2 cut(s) 29, 1271
MspI CCGG 2 cut(s) 867, 1425
MspR9I CCNGG 1 cut(s) 875
Mva1269I GAATGC 1 cut(s) 1124
MvaI CCWGG 1 cut(s) 875
MvnI CGCG 2 cut(s) 101, 234
MwoI GCNNNNNNNGC 4 cut(s) 148, 231, 269, 467
NcoI CCATGG 2 cut(s) 295, 816
NdeI CATATG 1 cut(s) 1405
NdeII GATC 7 cut(s) 34, 116, 376, 679, 751, 1084, 1258
NlaIII CATG 5 cut(s) 299, 703, 820, 889, 932
NlaIV GGNNCC 6 cut(s) 397, 753, 865, 950, 1175, 1401
NmuCI GTSAC 1 cut(s) 869
PciSI GCTCTTC 1 cut(s) 433
PcsI WCGNNNNNNNCGW 2 cut(s) 74, 276
PctI GAATGC 1 cut(s) 1124
PdmI GAANNNNTTC 2 cut(s) 798, 1289
PfeI GAWTC 8 cut(s) 103, 121, 245, 882, 1029, 1151, 1363, 1428
PflFI GACNNNGTC 1 cut(s) 278
PinAI ACCGGT 1 cut(s) 866
PkrI GCNGC 3 cut(s) 966, 1132, 1270
PleI GAGTC 4 cut(s) 398, 513, 577, 1360
PpsI GAGTC 4 cut(s) 398, 513, 577, 1360
Psp6I CCWGG 1 cut(s) 873
PspEI GGTNACC 1 cut(s) 869
PspGI CCWGG 1 cut(s) 873
PspN4I GGNNCC 6 cut(s) 397, 753, 865, 950, 1175, 1401
PspPI GGNCC 1 cut(s) 207
PsuI RGATCY 2 cut(s) 376, 751
PsyI GACNNNGTC 1 cut(s) 278
PvuII CAGCTG 2 cut(s) 29, 1271
RsaI GTAC 5 cut(s) 98, 865, 1020, 1110, 1265
RsaNI GTAC 5 cut(s) 97, 864, 1019, 1109, 1264
RseI CAYNNNNRTG 1 cut(s) 1190
Rsr2I CGGWCCG 1 cut(s) 207
RsrII CGGWCCG 1 cut(s) 207
SapI GCTCTTC 1 cut(s) 433
SaqAI TTAA 2 cut(s) 5, 1244
SatI GCNGC 3 cut(s) 965, 1131, 1269
Sau3AI GATC 7 cut(s) 34, 116, 376, 679, 751, 1084, 1258
Sau96I GGNCC 1 cut(s) 207
SchI GAGTC 4 cut(s) 398, 514, 578, 1361
ScrFI CCNGG 1 cut(s) 875
SduI GDGCHC 2 cut(s) 398, 770
SfaNI GCATC 1 cut(s) 234
SinI GGWCC 1 cut(s) 207
SmiMI CAYNNNNRTG 1 cut(s) 1190
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
SpeI ACTAGT 1 cut(s) 626
Sse9I AATT 3 cut(s) 72, 1040, 1080
SsiI CCGC 2 cut(s) 735, 935
SspMI CTAG 6 cut(s) 627, 893, 1181, 1326, 1436, 1443
StyD4I CCNGG 1 cut(s) 873
StyI CCWWGG 6 cut(s) 295, 341, 408, 816, 976, 1335
TaaI ACNGT 7 cut(s) 280, 556, 850, 1023, 1127, 1211, 1375
TaiI ACGT 3 cut(s) 63, 243, 1110
TaqI TCGA 6 cut(s) 57, 63, 119, 243, 333, 531
TaqII GACCGA 1 cut(s) 195
TasI AATT 3 cut(s) 72, 1040, 1080
TfiI GAWTC 8 cut(s) 103, 121, 245, 882, 1029, 1151, 1363, 1428
Tru1I TTAA 2 cut(s) 5, 1244
Tru9I TTAA 2 cut(s) 5, 1244
TscAI CASTG 5 cut(s) 559, 682, 1132, 1165, 1222
TseFI GTSAC 1 cut(s) 869
TseI GCWGC 3 cut(s) 964, 1130, 1268
Tsp45I GTSAC 1 cut(s) 869
TspDTI ATGAA 7 cut(s) 467, 539, 600, 720, 874, 974, 1208
TspGWI ACGGA 5 cut(s) 66, 199, 764, 1078, 1421
TspRI CASTG 5 cut(s) 559, 682, 1132, 1165, 1222
Tth111I GACNNNGTC 1 cut(s) 278
VpaK11BI GGWCC 1 cut(s) 207
XapI RAATTY 1 cut(s) 1040
XcmI CCANNNNNNNNNTGG 2 cut(s) 142, 406
XmiI GTMKAC 1 cut(s) 282
XmnI GAANNNNTTC 2 cut(s) 798, 1289
XspI CTAG 6 cut(s) 627, 893, 1181, 1326, 1436, 1443
ZraI GACGTC 2 cut(s) 61, 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.