Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0004362

Physical Location & Seq

Genomic Coordinates Forward (+)

33124982 .. 33127964

Gene Structure 2983 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1197200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1518 bp

ATGGCGAGTCGGAGCGAGGACCGGTTCGAAATAGCCTTCTTCGACGTGGAGACGACGGTGCCGACGCGACCCCGGCAGGGCTTCGCTATCTTGGAATTCGGGTCGATTCTCGTTTGCCCTAGGAAGCTGGTGGAGCTCGACAGCTACTCCACCCTGGTCCGACCCGCCGACCTCTCCGCCATCAACTCCTTGTCCGTGCGCTGCAACGGCATTACCCGCGACGCCGTTGTCGCCGCCCCTTCTTTTCAAGACATCGCCGATAAGGTCTACGACATTCTCCACGGACGTATATGGGCTGGTCACAACATTCTGAGGTTTGATTGTGCTCGGATTCGGGAGGCTTTCACACAAATTGGTCGGCCTGCTCCAGAACCGAAAGGTACAATTGATTCCTTGGCATTGTTGACACAGCGGTTCGGAAGGAGAGCTGGTAACATGAAGATGGCCACTCTTGCAACCTATTTCGGGCTTGGACAGCAGAAACACAGGAGTTTGGATGATGTTCGAATGAATCTTGAAGTTCTGAAATACTGTGCAACCGTTTTATTCTTGGAGTCGAGTCTCCCAGACATATTCACGGAGAACAGTTGGGTTTCTCCAAATGCTATCACAAGAAGTCGTAGTGATGGGAAATCTTCTGCTGAGGGGGCTCGCATAAACATGAATACTCAGCATGAGAATGGTCAGATGTTATCTTCTACAAATCAAAGAACAGGGGAGAATCATCCAATAATATCTCTTAGGGATAACAATACCGAAGAAGTCTCTAATCTGGTTGAATTGAGCACAGCTCGACCAGATCCCTTTGACATGGGCCCACTTGGCGATGTAGTGATGAAAGAGTTTAATCAGCCAGACATCACATTGAGAGAAATACCTGTGGAGGAGTCTATTGAGAGTTCTCCACCTGCTGCATTGGGGAGCTCCAATGGCACTGTGGAGTTTTTACAGCCTGATGAAGTTTCCATTCCTTCTATCTGTGCTTCCCTTGTTCCGTTGTATCGCGGTAGTCAGAGGATAAAGTTGTTGCACAAAGATGTAACTTTGCAGATTTGCTGTAGGCATATGAAATTGCGGTTTGGAATCAATGGAAAGTATTTTGATCATGCTGGCCGGCCACGATTGAATATTGTTGGTTACGCATCACCAAGTTTGTGCAAAGTTCTTGATGCATGTGATGGCATTGCACAAAAGTTGTCTATGGATTCTGGTAGCAGCTCCGAATGGAGGCGTGCTGTTTTCCGAAACGAGGGTTTCTATAACTGTCCTACAGTGAGATTACATATACCGACAGCAGTATGTGGGGATATTGCAATATACGCCACAGAGATATATCAGAAAGAGCCTTCGGGCACCGAGCAGAGGCTCGTCTTCACCAAGTTTGATTCTTCGGAACTTAGCACCCTTTTCAAACCGGGGACTTTTATGGATGCGTTCTTCTCCTTGGATCCGTACGACTATCAGCAGAGAGCAGGCATTAGGTTGGTGGCAAAGAAATTGATTCTTCATTCCAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006139 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0008408 GO:0009380 GO:0009987 GO:0016020 GO:0016787 GO:0016788 GO:0030312 GO:0032991 GO:0033554 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0071704 GO:0071944 GO:0090304 GO:0090305 GO:1901360 GO:1902494 GO:1905347 GO:1905348 GO:1990391

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RNase_T

Protein Analysis

506

Amino Acids

56.24

Weight (kDa)

6.58

Isoelectric Point (pI)

47.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNase_T	PF00929	13 - 175	4.8e-26	Exonuclease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004362)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74390 AT1G74390 AT1G74390 AT1G74390 AT5G07710 AT5G61390
fragaria_vesca	FvH4_5g14590 FvH4_5g14590 FvH4_5g14590
malus_domestica	MD06G1197200.v1.1 MD14G1204200.v1.1 MD14G1204300.v1.1 MD14G1204400.v1.1
prunus_persica	Prupe.5G197500_v2.0.a1 Prupe.5G197500_v2.0.a1 Prupe.5G197700_v2.0.a1
pyrus_communis	pycom06g17400 pycom14g16890
rosa_chinensis	RchiOBHm_Chr7g0181951 RchiOBHm_Chr7g0181981
rosa_laevigata	RLG00000005164
rosa_roxburghii	Rroxscaffold_3G00271650
rosa_rugosa	Rorug06G0444900
rosa_samantha	Rh7AG047200 Rh7BG046800 Rh7BG047100 Rh7CG047300 Rh7CG047400 Rh7DG046300 Rh7DG046400
rosa_wichuraiana	Rw0G019670 Rw7G003910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	916
AasI	GACNNNNNNGTC	1 cut(s)	227
Acc36I	ACCTGC	1 cut(s)	916
AccB1I	GGYRCC	2 cut(s)	58, 1352
AccI	GTMKAC	1 cut(s)	267
AccII	CGCG	3 cut(s)	67, 219, 1005
AciI	CCGC	7 cut(s)	165, 177, 217, 234, 412, 1005, 1075
AclWI	GGATC	3 cut(s)	794, 1442, 1455
AcoI	YGGCCR	3 cut(s)	444, 1111, 1115
AcsI	RAATTY	1 cut(s)	95
AcyI	GRCGYC	1 cut(s)	222
AfaI	GTAC	2 cut(s)	382, 1454
AfiI	CCNNNNNNNGG	5 cut(s)	77, 465, 1227, 1249, 1362
AgeI	ACCGGT	1 cut(s)	21
AgsI	TTSAA	5 cut(s)	248, 518, 779, 1126, 1411
AjiI	CACGTC	1 cut(s)	46
AjnI	CCWGG	1 cut(s)	153
AluBI	AGCT	7 cut(s)	127, 136, 144, 428, 791, 924, 1218
AluI	AGCT	7 cut(s)	127, 136, 144, 428, 791, 924, 1218
Alw21I	GWGCWC	4 cut(s)	138, 328, 788, 926
Alw26I	GTCTC	3 cut(s)	44, 566, 769
AlwI	GGATC	3 cut(s)	794, 1442, 1455
AoxI	GGCC	5 cut(s)	359, 444, 814, 1111, 1115
ApaI	GGGCCC	1 cut(s)	818
ApeKI	GCWGC	3 cut(s)	201, 911, 1215
ApoI	RAATTY	1 cut(s)	95
AsiGI	ACCGGT	1 cut(s)	21
AspA2I	CCTAGG	1 cut(s)	119
AspLEI	GCGC	1 cut(s)	201
AspS9I	GGNCC	4 cut(s)	19, 157, 814, 815
AsuC2I	CCSGG	2 cut(s)	73, 1416
AsuHPI	GGTGA	2 cut(s)	1137, 1366
AsuII	TTCGAA	2 cut(s)	27, 505
AvaII	GGWCC	2 cut(s)	19, 157
AvrII	CCTAGG	1 cut(s)	119
BaeGI	GKGCMC	2 cut(s)	818, 1355
BalI	TGGCCA	1 cut(s)	446
BamHI	GGATCC	1 cut(s)	1447
BanI	GGYRCC	2 cut(s)	58, 1352
BanII	GRGCYC	4 cut(s)	138, 652, 818, 926
BbsI	GAAGAC	1 cut(s)	1363
Bbv12I	GWGCWC	4 cut(s)	138, 328, 788, 926
BbvCI	CCTCAGC	1 cut(s)	642
BbvI	GCAGC	3 cut(s)	188, 898, 1227
BccI	CCATC	4 cut(s)	188, 436, 620, 1172
BceAI	ACGGC	2 cut(s)	209, 223
BciT130I	CCWGG	1 cut(s)	155
BclI	TGATCA	1 cut(s)	1102
BcnI	CCSGG	2 cut(s)	73, 1416
BcoDI	GTCTC	3 cut(s)	44, 566, 769
BfaI	CTAG	1 cut(s)	120
BfmI	CTRYAG	2 cut(s)	1057, 1269
BfuAI	ACCTGC	1 cut(s)	916
BglI	GCCNNNNNGGC	1 cut(s)	822
BisI	GCNGC	4 cut(s)	202, 234, 912, 1216
BlnI	CCTAGG	1 cut(s)	119
BlsI	GCNGC	4 cut(s)	203, 235, 913, 1217
Bme1390I	CCNGG	3 cut(s)	73, 155, 1416
Bme18I	GGWCC	2 cut(s)	19, 157
BmgBI	CACGTC	1 cut(s)	46
BmgT120I	GGNCC	4 cut(s)	19, 157, 814, 815
BmiI	GGNNCC	4 cut(s)	60, 816, 1354, 1449
BmrFI	CCNGG	3 cut(s)	73, 155, 1416
BmsI	GCATC	3 cut(s)	1151, 1159, 1420
BpiI	GAAGAC	1 cut(s)	1363
BplI	GAGNNNNNCTC	2 cut(s)	775, 807
BpmI	CTGGAG	1 cut(s)	351
Bpu10I	CCTNAGC	1 cut(s)	642
Bpu14I	TTCGAA	2 cut(s)	27, 505
BpuMI	CCSGG	2 cut(s)	73, 1416
BsaHI	GRCGYC	1 cut(s)	222
BsaJI	CCNNGG	7 cut(s)	71, 119, 153, 280, 393, 1415, 1443
BsaWI	WCCGGW	1 cut(s)	21
BsaXI	ACNNNNNCTCC	4 cut(s)	131, 161, 415, 445
Bsc4I	CCNNNNNNNGG	5 cut(s)	77, 465, 1227, 1249, 1362
Bse118I	RCCGGY	2 cut(s)	21, 1113
Bse3DI	GCAATG	1 cut(s)	1182
BseBI	CCWGG	1 cut(s)	155
BseDI	CCNNGG	7 cut(s)	71, 119, 153, 280, 393, 1415, 1443
BseGI	GGATG	3 cut(s)	502, 724, 1435
BseLI	CCNNNNNNNGG	5 cut(s)	77, 465, 1227, 1249, 1362
BseMI	GCAATG	1 cut(s)	1182
BseMII	CTCAG	3 cut(s)	302, 633, 683
BseRI	GAGGAG	1 cut(s)	899
BseSI	GKGCMC	2 cut(s)	818, 1355
BseXI	GCAGC	3 cut(s)	188, 898, 1227
Bsh1236I	CGCG	3 cut(s)	67, 219, 1005
BshFI	GGCC	5 cut(s)	361, 446, 816, 1113, 1117
BshNI	GGYRCC	2 cut(s)	58, 1352
BshTI	ACCGGT	1 cut(s)	21
BsiHKAI	GWGCWC	4 cut(s)	138, 328, 788, 926
BsiSI	CCGG	4 cut(s)	22, 73, 1114, 1415
BsiWI	CGTACG	1 cut(s)	1452
BslFI	GGGAC	1 cut(s)	1432
BslI	CCNNNNNNNGG	5 cut(s)	77, 465, 1227, 1249, 1362
BsmAI	GTCTC	3 cut(s)	44, 566, 769
BsmBI	CGTCTC	1 cut(s)	44
BsmFI	GGGAC	1 cut(s)	1432
BsnI	GGCC	5 cut(s)	361, 446, 816, 1113, 1117
Bsp119I	TTCGAA	2 cut(s)	27, 505
Bsp120I	GGGCCC	1 cut(s)	814
Bsp1286I	GDGCHC	7 cut(s)	138, 328, 652, 788, 818, 926, 1355
Bsp143I	GATC	3 cut(s)	799, 1102, 1447
BspACI	CCGC	7 cut(s)	165, 177, 217, 234, 412, 1005, 1075
BspANI	GGCC	5 cut(s)	361, 446, 816, 1113, 1117
BspCNI	CTCAG	3 cut(s)	303, 634, 682
BspFNI	CGCG	3 cut(s)	67, 219, 1005
BspLI	GGNNCC	4 cut(s)	60, 816, 1354, 1449
BspMI	ACCTGC	1 cut(s)	916
BspPI	GGATC	3 cut(s)	794, 1442, 1455
BspT104I	TTCGAA	2 cut(s)	27, 505
BspT107I	GGYRCC	2 cut(s)	58, 1352
BsrDI	GCAATG	1 cut(s)	1182
BsrFI	RCCGGY	2 cut(s)	21, 1113
BssAI	RCCGGY	2 cut(s)	21, 1113
BssECI	CCNNGG	7 cut(s)	71, 119, 153, 280, 393, 1415, 1443
BssMI	GATC	3 cut(s)	799, 1102, 1447
BssNI	GRCGYC	1 cut(s)	222
BssT1I	CCWWGG	3 cut(s)	119, 393, 1443
Bst2UI	CCWGG	1 cut(s)	155
Bst4CI	ACNGT	7 cut(s)	58, 533, 541, 587, 937, 1265, 1273
BstACI	GRCGYC	1 cut(s)	222
BstBI	TTCGAA	2 cut(s)	27, 505
BstC8I	GCNNGC	6 cut(s)	363, 652, 1111, 1115, 1233, 1474
BstDEI	CTNAG	5 cut(s)	311, 642, 669, 740, 1397
BstDSI	CCRYGG	1 cut(s)	280
BstF5I	GGATG	3 cut(s)	502, 724, 1435
BstFNI	CGCG	3 cut(s)	67, 219, 1005
BstHHI	GCGC	1 cut(s)	201
BstKTI	GATC	3 cut(s)	802, 1105, 1450
BstMAI	GTCTC	3 cut(s)	44, 566, 769
BstMBI	GATC	3 cut(s)	799, 1102, 1447
BstNI	CCWGG	1 cut(s)	155
BstNSI	RCATGY	1 cut(s)	1176
BstSCI	CCNGG	3 cut(s)	71, 153, 1414
BstSFI	CTRYAG	2 cut(s)	1057, 1269
BstSLI	GKGCMC	2 cut(s)	818, 1355
BstUI	CGCG	3 cut(s)	67, 219, 1005
BstV1I	GCAGC	3 cut(s)	188, 898, 1227
BstV2I	GAAGAC	1 cut(s)	1363
BstX2I	RGATCY	2 cut(s)	799, 1447
BstYI	RGATCY	2 cut(s)	799, 1447
BsuRI	GGCC	5 cut(s)	361, 446, 816, 1113, 1117
BtgI	CCRYGG	1 cut(s)	280
BtgZI	GCGATG	2 cut(s)	238, 840
BtrI	CACGTC	1 cut(s)	46
BtsCI	GGATG	3 cut(s)	502, 724, 1435
BtsIMutI	CAGTG	2 cut(s)	933, 1278
BveI	ACCTGC	1 cut(s)	916
Cac8I	GCNNGC	6 cut(s)	363, 652, 1111, 1115, 1233, 1474
CfoI	GCGC	1 cut(s)	201
Cfr10I	RCCGGY	2 cut(s)	21, 1113
Cfr13I	GGNCC	4 cut(s)	19, 157, 814, 815
CseI	GACGC	2 cut(s)	73, 230
Csp6I	GTAC	2 cut(s)	381, 1453
CspAI	ACCGGT	1 cut(s)	21
CviAII	CATG	6 cut(s)	436, 661, 674, 811, 1106, 1173
CviQI	GTAC	2 cut(s)	381, 1453
DdeI	CTNAG	5 cut(s)	311, 642, 669, 740, 1397
DpnI	GATC	3 cut(s)	801, 1104, 1449
DpnII	GATC	3 cut(s)	799, 1102, 1447
DrdI	GACNNNNNNGTC	1 cut(s)	227
DseDI	GACNNNNNNGTC	1 cut(s)	227
EaeI	YGGCCR	3 cut(s)	444, 1111, 1115
EciI	GGCGGA	1 cut(s)	166
Ecl136II	GAGCTC	2 cut(s)	136, 924
Eco130I	CCWWGG	3 cut(s)	119, 393, 1443
Eco24I	GRGCYC	4 cut(s)	138, 652, 818, 926
Eco47I	GGWCC	2 cut(s)	19, 157
Eco53kI	GAGCTC	2 cut(s)	136, 924
EcoICRI	GAGCTC	2 cut(s)	136, 924
EcoRI	GAATTC	1 cut(s)	95
EcoRII	CCWGG	1 cut(s)	153
EcoT14I	CCWWGG	3 cut(s)	119, 393, 1443
EcoT22I	ATGCAT	1 cut(s)	1174
EcoT38I	GRGCYC	4 cut(s)	138, 652, 818, 926
ErhI	CCWWGG	3 cut(s)	119, 393, 1443
Esp3I	CGTCTC	1 cut(s)	44
FaeI	CATG	6 cut(s)	439, 664, 677, 814, 1109, 1176
FaqI	GGGAC	1 cut(s)	1432
FatI	CATG	6 cut(s)	435, 660, 673, 810, 1105, 1172
FauI	CCCGC	2 cut(s)	172, 224
FauNDI	CATATG	1 cut(s)	1065
FbaI	TGATCA	1 cut(s)	1102
FblI	GTMKAC	1 cut(s)	267
Fnu4HI	GCNGC	4 cut(s)	202, 234, 912, 1216
FokI	GGATG	3 cut(s)	509, 711, 1442
FriOI	GRGCYC	4 cut(s)	138, 652, 818, 926
FseI	GGCCGGCC	1 cut(s)	1117
Fsp4HI	GCNGC	4 cut(s)	202, 234, 912, 1216
FspBI	CTAG	1 cut(s)	120
GlaI	GCGC	1 cut(s)	200
GluI	GCNGC	4 cut(s)	202, 234, 912, 1216
GsuI	CTGGAG	1 cut(s)	351
HaeIII	GGCC	5 cut(s)	361, 446, 816, 1113, 1117
HapII	CCGG	4 cut(s)	22, 73, 1114, 1415
HgaI	GACGC	2 cut(s)	73, 230
HhaI	GCGC	1 cut(s)	201
Hin1I	GRCGYC	1 cut(s)	222
Hin1II	CATG	6 cut(s)	439, 664, 677, 814, 1109, 1176
Hin6I	GCGC	1 cut(s)	199
HinP1I	GCGC	1 cut(s)	199
HincII	GTYRAC	1 cut(s)	405
HindII	GTYRAC	1 cut(s)	405
HpaII	CCGG	4 cut(s)	22, 73, 1114, 1415
HphI	GGTGA	2 cut(s)	1137, 1366
Hpy166II	GTNNAC	2 cut(s)	268, 405
Hpy188III	TCNNGA	5 cut(s)	248, 335, 368, 515, 1166
Hpy8I	GTNNAC	2 cut(s)	268, 405
Hpy99I	CGWCG	4 cut(s)	47, 58, 67, 224
HpyAV	CCTTC	5 cut(s)	46, 249, 414, 981, 1356
HpyCH4III	ACNGT	7 cut(s)	58, 533, 541, 587, 937, 1265, 1273
HpyCH4IV	ACGT	2 cut(s)	45, 286
HpyF3I	CTNAG	5 cut(s)	311, 642, 669, 740, 1397
HpySE526I	ACGT	2 cut(s)	45, 286
Hsp92I	GRCGYC	1 cut(s)	222
Hsp92II	CATG	6 cut(s)	439, 664, 677, 814, 1109, 1176
HspAI	GCGC	1 cut(s)	199
KroI	GCCGGC	1 cut(s)	1113
KroNI	GCCGGC	1 cut(s)	1115
Ksp22I	TGATCA	1 cut(s)	1102
Kzo9I	GATC	3 cut(s)	799, 1102, 1447
LmnI	GCTCC	6 cut(s)	12, 133, 370, 921, 929, 1223
Lsp1109I	GCAGC	3 cut(s)	188, 898, 1227
LweI	GCATC	3 cut(s)	1151, 1159, 1420
MaeI	CTAG	1 cut(s)	120
MaeII	ACGT	2 cut(s)	45, 286
MaeIII	GTNAC	4 cut(s)	299, 431, 1039, 1136
MalI	GATC	3 cut(s)	801, 1104, 1449
MboI	GATC	3 cut(s)	799, 1102, 1447
MboII	GAAGA	9 cut(s)	31, 451, 627, 687, 770, 1363, 1380, 1429, 1496
MfeI	CAATTG	2 cut(s)	384, 1513
MflI	RGATCY	2 cut(s)	799, 1447
MhlI	GDGCHC	7 cut(s)	138, 328, 652, 788, 818, 926, 1355
MlsI	TGGCCA	1 cut(s)	446
MluCI	AATT	7 cut(s)	95, 351, 384, 779, 1070, 1496, 1513
MluNI	TGGCCA	1 cut(s)	446
MlyI	GAGTC	4 cut(s)	16, 563, 568, 896
MmeI	TCCRAC	1 cut(s)	184
Mox20I	TGGCCA	1 cut(s)	446
Mph1103I	ATGCAT	1 cut(s)	1174
MroNI	GCCGGC	1 cut(s)	1113
MscI	TGGCCA	1 cut(s)	446
MseI	TTAA	1 cut(s)	846
MslI	CAYNNNNRTG	4 cut(s)	440, 659, 678, 1035
Msp20I	TGGCCA	1 cut(s)	446
MspA1I	CMGCKG	1 cut(s)	412
MspI	CCGG	4 cut(s)	22, 73, 1114, 1415
MspR9I	CCNGG	3 cut(s)	73, 155, 1416
MunI	CAATTG	2 cut(s)	384, 1513
MvaI	CCWGG	1 cut(s)	155
MvnI	CGCG	3 cut(s)	67, 219, 1005
NaeI	GCCGGC	1 cut(s)	1115
NciI	CCSGG	2 cut(s)	73, 1416
NdeI	CATATG	1 cut(s)	1065
NdeII	GATC	3 cut(s)	799, 1102, 1447
NgoMIV	GCCGGC	1 cut(s)	1113
NlaIII	CATG	6 cut(s)	439, 664, 677, 814, 1109, 1176
NlaIV	GGNNCC	4 cut(s)	60, 816, 1354, 1449
NmuCI	GTSAC	1 cut(s)	299
NsiI	ATGCAT	1 cut(s)	1174
NspI	RCATGY	1 cut(s)	1176
NspV	TTCGAA	2 cut(s)	27, 505
PaqCI	CACCTGC	1 cut(s)	916
PcsI	WCGNNNNNNNCGW	1 cut(s)	59
PdiI	GCCGGC	1 cut(s)	1115
PfeI	GAWTC	9 cut(s)	106, 331, 389, 511, 721, 1083, 1205, 1385, 1501
Pfl23II	CGTACG	1 cut(s)	1452
PinAI	ACCGGT	1 cut(s)	21
PkrI	GCNGC	4 cut(s)	203, 235, 913, 1217
PleI	GAGTC	4 cut(s)	15, 562, 567, 895
PpsI	GAGTC	4 cut(s)	15, 562, 567, 895
Psp124BI	GAGCTC	2 cut(s)	138, 926
Psp6I	CCWGG	1 cut(s)	153
PspGI	CCWGG	1 cut(s)	153
PspLI	CGTACG	1 cut(s)	1452
PspN4I	GGNNCC	4 cut(s)	60, 816, 1354, 1449
PspOMI	GGGCCC	1 cut(s)	814
PspPI	GGNCC	4 cut(s)	19, 157, 814, 815
PsuI	RGATCY	2 cut(s)	799, 1447
RigI	GGCCGGCC	1 cut(s)	1117
RsaI	GTAC	2 cut(s)	382, 1454
RsaNI	GTAC	2 cut(s)	381, 1453
RseI	CAYNNNNRTG	4 cut(s)	440, 659, 678, 1035
SacI	GAGCTC	2 cut(s)	138, 926
SaqAI	TTAA	1 cut(s)	846
SatI	GCNGC	4 cut(s)	202, 234, 912, 1216
Sau3AI	GATC	3 cut(s)	799, 1102, 1447
Sau96I	GGNCC	4 cut(s)	19, 157, 814, 815
SchI	GAGTC	4 cut(s)	16, 563, 568, 896
ScrFI	CCNGG	3 cut(s)	73, 155, 1416
SduI	GDGCHC	7 cut(s)	138, 328, 652, 788, 818, 926, 1355
SfaNI	GCATC	3 cut(s)	1151, 1159, 1420
SfcI	CTRYAG	2 cut(s)	1057, 1269
SfuI	TTCGAA	2 cut(s)	27, 505
SinI	GGWCC	2 cut(s)	19, 157
SmiMI	CAYNNNNRTG	4 cut(s)	440, 659, 678, 1035
Sse9I	AATT	7 cut(s)	95, 351, 384, 779, 1070, 1496, 1513
SsiI	CCGC	7 cut(s)	165, 177, 217, 234, 412, 1005, 1075
SspI	AATATT	1 cut(s)	1129
SspMI	CTAG	1 cut(s)	120
SstI	GAGCTC	2 cut(s)	138, 926
StyD4I	CCNGG	3 cut(s)	71, 153, 1414
StyI	CCWWGG	3 cut(s)	119, 393, 1443
TaaI	ACNGT	7 cut(s)	58, 533, 541, 587, 937, 1265, 1273
TaiI	ACGT	2 cut(s)	48, 289
TaqI	TCGA	7 cut(s)	27, 42, 104, 138, 505, 557, 793
TasI	AATT	7 cut(s)	95, 351, 384, 779, 1070, 1496, 1513
TauI	GCSGC	1 cut(s)	236
TfiI	GAWTC	9 cut(s)	106, 331, 389, 511, 721, 1083, 1205, 1385, 1501
Tru1I	TTAA	1 cut(s)	846
Tru9I	TTAA	1 cut(s)	846
TscAI	CASTG	2 cut(s)	940, 1278
TseFI	GTSAC	1 cut(s)	299
TseI	GCWGC	3 cut(s)	201, 911, 1215
Tsp45I	GTSAC	1 cut(s)	299
TspDTI	ATGAA	7 cut(s)	452, 524, 677, 851, 972, 1082, 1496
TspGWI	ACGGA	5 cut(s)	184, 297, 593, 984, 1440
TspRI	CASTG	2 cut(s)	940, 1278
VpaK11BI	GGWCC	2 cut(s)	19, 157
XapI	RAATTY	1 cut(s)	95
XceI	RCATGY	1 cut(s)	1176
XcmI	CCANNNNNNNNNTGG	1 cut(s)	934
XmaJI	CCTAGG	1 cut(s)	119
XmiI	GTMKAC	1 cut(s)	267
XspI	CTAG	1 cut(s)	120
Zsp2I	ATGCAT	1 cut(s)	1174

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.