MD14G1204300.v1.1

EXOIII

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004362
Physical Location & Seq
Forward (+)
29237446 .. 29240172
2727 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1204300.v1.1.491

Sequence Viewer

Length: 1458 bp
ATGGCGACTCGGAGCGAGGACCGGTTTGAAATAGCCTTCTTCGACGTGGAGACGACGGTGCCGACCCGACCCGGACAGGGCTTCAACATCTTGGAATTCGGGTCGATTCTCGTTTGCCCTAGGAAGCTGGTGGAGCTCGAGAGCTACTCCACCCTGATTCGACCCGCCGACCTCTCCGCCATCGATTCCCTGTCCGTCCGTTGCAACGGCATTACCCGCGACGCCGTCATTGCCGCCCCTTCTTTTGAAGACATCGCCGATAGGGTCTACGACATTCTCCACGAACGTATATGGGCTGGTCACAACATTCTGAGGTTTGATTGTTATCGGATTCGGGAGGCTTTCGCACGCATTGGTCGGCCTGCGCCAGAACCGAAAGGTACCATTGATTCGTTGGCATTGTTGACACAGCGGTTCGGAAGGAGAGCTGGTAACATGAAGATGGCCTCCCTTGCAACCTATTTCGGGCTTGGACAGCAGACACACAGGAGTTTGGATGATGTTCGAATGAATCTTGAAGTTCTGAAGTATTGTGCAACCGTGTTACTCTTGGAGTCGAGCCTCCCAGACATATTCACAGAGAATAGTTGGGTTTCTCCAAATGCTACCACAAGAAGTCGTAGTGATGGGAAATCATCTTCTACAAATCAAATAACAGGGGACAATCATCCAATAACATCTCTTAGGACTACCAAAGAAGTCTCTAATCTGGTTGAATCTAGCACAGCTCGACCAGATCCCTTTGACATGGGCCCACTTAGCAATGAAGTGATGAAAGAGGTGAATCAGCCAGATATCGCCATGGTAGAAATACCTGTGCAAGAGTCTGTTGAGAGTTCTTCCCATTTGGCTGCATTGGGGAGTTCCAATGGCACTATGGAGTTTTTGCAGCCTGATGAAATTTCTATTCCTTCAATCTGTGCTTCCCTTGTTCTGTTGTATCATGGGCGTCAGAGGATAAAGTTGTTACACAAAGATGTAAGTTTGCAGCTTTGCTGTAGGCATATGAAATTGCGGTTTGGGATCAATGAAAAGTTTTTTGATCATGCTGGCCAGCCACGATTGAATATTGTTTGTTACGCATCACAAAATTTGTGCAAAGTTCTTGATGCATGTGATGGCATTGCACAAAAGTTGTCTATGGATTCCGGTAGCAGCTCCGAATGGAGGCCTGTAGTGATCCGAAAAGAGGGTTTCAATAACTATCCTACAGTGAGATTACACATACAGACAGCAGTATGTGGGGATATTGCAATATACGCCACAGAGATATATCAAAAAGAACCTTCTGGCGCCGAGGAGAGGCTCATCTTCACCAAGTTTGATGCTTCAGCACTTAGCACCCTTTTCAAACCGCAGACTTTTATGGATGCGTTCTTCTCCTTGGATCCGTATGACCATCAGGAGAGTGCAGGCATTAGGTTGGTGGCAAAGAAATTGATTCTTCATTCCAATTGA

Protein Analysis

486

Amino Acids

54.11

Weight (kDa)

5.99

Isoelectric Point (pI)

47.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 13 - 175 4.3e-26 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 380
AccB1I GGYRCC 3 cut(s) 58, 380, 1292
AccI GTMKAC 1 cut(s) 267
AccII CGCG 1 cut(s) 219
AciI CCGC 7 cut(s) 165, 177, 217, 234, 412, 1015, 1355
AclWI GGATC 5 cut(s) 731, 1031, 1174, 1382, 1395
AcoI YGGCCR 1 cut(s) 1051
AcsI RAATTY 3 cut(s) 95, 900, 1090
AcuI CTGAAG 2 cut(s) 545, 1314
AcyI GRCGYC 3 cut(s) 222, 949, 1293
AfaI GTAC 1 cut(s) 382
AfiI CCNNNNNNNGG 5 cut(s) 77, 465, 1167, 1189, 1302
AgeI ACCGGT 1 cut(s) 21
AgsI TTSAA 9 cut(s) 29, 85, 248, 518, 716, 915, 1066, 1198, 1351
AjiI CACGTC 1 cut(s) 46
AluBI AGCT 7 cut(s) 127, 136, 144, 428, 728, 991, 1158
AluI AGCT 7 cut(s) 127, 136, 144, 428, 728, 991, 1158
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 2 cut(s) 44, 706
AlwI GGATC 5 cut(s) 731, 1031, 1174, 1382, 1395
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 5 cut(s) 359, 444, 751, 1051, 1169
ApaI GGGCCC 1 cut(s) 755
ApeKI GCWGC 4 cut(s) 851, 889, 988, 1155
ApoI RAATTY 3 cut(s) 95, 900, 1090
AsiGI ACCGGT 1 cut(s) 21
Asp718I GGTACC 1 cut(s) 380
AspA2I CCTAGG 1 cut(s) 119
AspLEI GCGC 2 cut(s) 367, 1295
AspS9I GGNCC 3 cut(s) 19, 751, 752
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 2 cut(s) 793, 1306
AsuII TTCGAA 1 cut(s) 505
AvaI CYCGRG 1 cut(s) 137
AvaII GGWCC 1 cut(s) 19
AvrII CCTAGG 1 cut(s) 119
BaeGI GKGCMC 1 cut(s) 755
BalI TGGCCA 1 cut(s) 1053
BamHI GGATCC 1 cut(s) 1387
BanI GGYRCC 3 cut(s) 58, 380, 1292
BanII GRGCYC 2 cut(s) 138, 755
BbsI GAAGAC 1 cut(s) 255
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 4 cut(s) 838, 901, 1000, 1167
BccI CCATC 5 cut(s) 188, 436, 620, 1112, 1407
BceAI ACGGC 2 cut(s) 209, 223
BclI TGATCA 1 cut(s) 1042
BcnI CCSGG 1 cut(s) 72
BcoDI GTCTC 2 cut(s) 44, 706
BfaI CTAG 2 cut(s) 120, 720
BfmI CTRYAG 3 cut(s) 997, 1173, 1209
BfoI RGCGCY 1 cut(s) 1296
BisI GCNGC 5 cut(s) 234, 852, 890, 989, 1156
BlnI CCTAGG 1 cut(s) 119
BlsI GCNGC 5 cut(s) 235, 853, 891, 990, 1157
Bme1390I CCNGG 1 cut(s) 72
Bme18I GGWCC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 137
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 3 cut(s) 19, 751, 752
BmiI GGNNCC 5 cut(s) 60, 382, 753, 1294, 1389
BmrFI CCNGG 1 cut(s) 72
BmsI GCATC 4 cut(s) 1091, 1099, 1315, 1360
BpiI GAAGAC 1 cut(s) 255
BplI GAGNNNNNCTC 2 cut(s) 131, 163
Bpu14I TTCGAA 1 cut(s) 505
BpuMI CCSGG 1 cut(s) 72
Bsa29I ATCGAT 1 cut(s) 183
BsaBI GATNNNNATC 1 cut(s) 324
BsaHI GRCGYC 3 cut(s) 222, 949, 1293
BsaJI CCNNGG 4 cut(s) 119, 801, 1296, 1383
BsaWI WCCGGW 2 cut(s) 21, 1148
BsaXI ACNNNNNCTCC 2 cut(s) 415, 445
Bsc4I CCNNNNNNNGG 5 cut(s) 77, 465, 1167, 1189, 1302
Bse118I RCCGGY 1 cut(s) 21
Bse3DI GCAATG 3 cut(s) 228, 769, 1122
Bse8I GATNNNNATC 1 cut(s) 324
BseCI ATCGAT 1 cut(s) 183
BseDI CCNNGG 4 cut(s) 119, 801, 1296, 1383
BseGI GGATG 3 cut(s) 502, 667, 1375
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 5 cut(s) 77, 465, 1167, 1189, 1302
BseMI GCAATG 3 cut(s) 228, 769, 1122
BseMII CTCAG 1 cut(s) 302
BseRI GAGGAG 1 cut(s) 1313
BseSI GKGCMC 1 cut(s) 755
BseXI GCAGC 4 cut(s) 838, 901, 1000, 1167
BsgI GTGCAG 1 cut(s) 1431
Bsh1236I CGCG 1 cut(s) 219
BshFI GGCC 5 cut(s) 361, 446, 753, 1053, 1171
BshNI GGYRCC 3 cut(s) 58, 380, 1292
BshTI ACCGGT 1 cut(s) 21
BshVI ATCGAT 1 cut(s) 183
BsiHKAI GWGCWC 1 cut(s) 138
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 3 cut(s) 22, 72, 1149
BslFI GGGAC 1 cut(s) 674
BslI CCNNNNNNNGG 5 cut(s) 77, 465, 1167, 1189, 1302
BsmAI GTCTC 2 cut(s) 44, 706
BsmBI CGTCTC 1 cut(s) 44
BsmFI GGGAC 1 cut(s) 674
BsnI GGCC 5 cut(s) 361, 446, 753, 1053, 1171
BsoBI CYCGRG 1 cut(s) 137
Bsp119I TTCGAA 1 cut(s) 505
Bsp120I GGGCCC 1 cut(s) 751
Bsp1286I GDGCHC 2 cut(s) 138, 755
Bsp143I GATC 5 cut(s) 736, 1023, 1042, 1179, 1387
Bsp19I CCATGG 1 cut(s) 801
BspACI CCGC 7 cut(s) 165, 177, 217, 234, 412, 1015, 1355
BspANI GGCC 5 cut(s) 361, 446, 753, 1053, 1171
BspCNI CTCAG 1 cut(s) 303
BspDI ATCGAT 1 cut(s) 183
BspFNI CGCG 1 cut(s) 219
BspLI GGNNCC 5 cut(s) 60, 382, 753, 1294, 1389
BspPI GGATC 5 cut(s) 731, 1031, 1174, 1382, 1395
BspT104I TTCGAA 1 cut(s) 505
BspT107I GGYRCC 3 cut(s) 58, 380, 1292
BsrDI GCAATG 3 cut(s) 228, 769, 1122
BsrFI RCCGGY 1 cut(s) 21
BssAI RCCGGY 1 cut(s) 21
BssECI CCNNGG 4 cut(s) 119, 801, 1296, 1383
BssMI GATC 5 cut(s) 736, 1023, 1042, 1179, 1387
BssNI GRCGYC 3 cut(s) 222, 949, 1293
BssT1I CCWWGG 3 cut(s) 119, 801, 1383
Bst4CI ACNGT 3 cut(s) 58, 541, 1213
BstACI GRCGYC 3 cut(s) 222, 949, 1293
BstBI TTCGAA 1 cut(s) 505
BstC8I GCNNGC 5 cut(s) 349, 363, 1051, 1055, 1414
BstDEI CTNAG 4 cut(s) 311, 683, 758, 1337
BstDSI CCRYGG 1 cut(s) 801
BstF5I GGATG 3 cut(s) 502, 667, 1375
BstFNI CGCG 1 cut(s) 219
BstH2I RGCGCY 1 cut(s) 1296
BstHHI GCGC 2 cut(s) 367, 1295
BstKTI GATC 5 cut(s) 739, 1026, 1045, 1182, 1390
BstMAI GTCTC 2 cut(s) 44, 706
BstMBI GATC 5 cut(s) 736, 1023, 1042, 1179, 1387
BstMWI GCNNNNNNNGC 7 cut(s) 133, 216, 230, 452, 475, 759, 1259
BstNSI RCATGY 1 cut(s) 1116
BstSCI CCNGG 1 cut(s) 70
BstSFI CTRYAG 3 cut(s) 997, 1173, 1209
BstSLI GKGCMC 1 cut(s) 755
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 4 cut(s) 838, 901, 1000, 1167
BstV2I GAAGAC 1 cut(s) 255
BstX2I RGATCY 2 cut(s) 736, 1387
BstYI RGATCY 2 cut(s) 736, 1387
Bsu15I ATCGAT 1 cut(s) 183
BsuRI GGCC 5 cut(s) 361, 446, 753, 1053, 1171
BsuTUI ATCGAT 1 cut(s) 183
BtgI CCRYGG 1 cut(s) 801
BtgZI GCGATG 1 cut(s) 238
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 3 cut(s) 502, 667, 1375
BtsIMutI CAGTG 1 cut(s) 1218
Cac8I GCNNGC 5 cut(s) 349, 363, 1051, 1055, 1414
CfoI GCGC 2 cut(s) 367, 1295
Cfr10I RCCGGY 1 cut(s) 21
Cfr13I GGNCC 3 cut(s) 19, 751, 752
ClaI ATCGAT 1 cut(s) 183
CseI GACGC 2 cut(s) 230, 938
Csp6I GTAC 1 cut(s) 381
CspAI ACCGGT 1 cut(s) 21
CviAII CATG 6 cut(s) 436, 748, 802, 944, 1046, 1113
CviQI GTAC 1 cut(s) 381
DdeI CTNAG 4 cut(s) 311, 683, 758, 1337
DinI GGCGCC 1 cut(s) 1294
DpnI GATC 5 cut(s) 738, 1025, 1044, 1181, 1389
DpnII GATC 5 cut(s) 736, 1023, 1042, 1179, 1387
EaeI YGGCCR 1 cut(s) 1051
EciI GGCGGA 1 cut(s) 166
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 3 cut(s) 119, 801, 1383
Eco147I AGGCCT 1 cut(s) 1171
Eco24I GRGCYC 2 cut(s) 138, 755
Eco32I GATATC 1 cut(s) 796
Eco47I GGWCC 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 136
Eco57I CTGAAG 2 cut(s) 545, 1314
Eco88I CYCGRG 1 cut(s) 137
EcoICRI GAGCTC 1 cut(s) 136
EcoRI GAATTC 1 cut(s) 95
EcoRV GATATC 1 cut(s) 796
EcoT14I CCWWGG 3 cut(s) 119, 801, 1383
EcoT22I ATGCAT 1 cut(s) 1114
EcoT38I GRGCYC 2 cut(s) 138, 755
EgeI GGCGCC 1 cut(s) 1294
EheI GGCGCC 1 cut(s) 1294
ErhI CCWWGG 3 cut(s) 119, 801, 1383
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 6 cut(s) 439, 751, 805, 947, 1049, 1116
FaqI GGGAC 1 cut(s) 674
FatI CATG 6 cut(s) 435, 747, 801, 943, 1045, 1112
FauI CCCGC 2 cut(s) 172, 224
FauNDI CATATG 1 cut(s) 1005
FbaI TGATCA 1 cut(s) 1042
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 5 cut(s) 234, 852, 890, 989, 1156
FokI GGATG 3 cut(s) 509, 654, 1382
FriOI GRGCYC 2 cut(s) 138, 755
Fsp4HI GCNGC 5 cut(s) 234, 852, 890, 989, 1156
FspBI CTAG 2 cut(s) 120, 720
GlaI GCGC 2 cut(s) 366, 1294
GluI GCNGC 5 cut(s) 234, 852, 890, 989, 1156
HaeII RGCGCY 1 cut(s) 1296
HaeIII GGCC 5 cut(s) 361, 446, 753, 1053, 1171
HapII CCGG 3 cut(s) 22, 72, 1149
HgaI GACGC 2 cut(s) 230, 938
HhaI GCGC 2 cut(s) 367, 1295
Hin1I GRCGYC 3 cut(s) 222, 949, 1293
Hin1II CATG 6 cut(s) 439, 751, 805, 947, 1049, 1116
Hin6I GCGC 2 cut(s) 365, 1293
HinP1I GCGC 2 cut(s) 365, 1293
HincII GTYRAC 1 cut(s) 405
HindII GTYRAC 1 cut(s) 405
HpaII CCGG 3 cut(s) 22, 72, 1149
HphI GGTGA 2 cut(s) 793, 1306
Hpy166II GTNNAC 2 cut(s) 268, 405
Hpy188I TCNGA 8 cut(s) 12, 312, 330, 419, 525, 954, 1162, 1184
Hpy188III TCNNGA 5 cut(s) 139, 335, 515, 1106, 1403
Hpy8I GTNNAC 2 cut(s) 268, 405
Hpy99I CGWCG 3 cut(s) 47, 58, 224
HpyAV CCTTC 5 cut(s) 46, 249, 414, 921, 1296
HpyCH4III ACNGT 3 cut(s) 58, 541, 1213
HpyCH4IV ACGT 2 cut(s) 45, 286
HpyF10VI GCNNNNNNNGC 7 cut(s) 133, 216, 230, 452, 475, 759, 1259
HpyF3I CTNAG 4 cut(s) 311, 683, 758, 1337
HpySE526I ACGT 2 cut(s) 45, 286
Hsp92I GRCGYC 3 cut(s) 222, 949, 1293
Hsp92II CATG 6 cut(s) 439, 751, 805, 947, 1049, 1116
HspAI GCGC 2 cut(s) 365, 1293
KasI GGCGCC 1 cut(s) 1292
KpnI GGTACC 1 cut(s) 384
Ksp22I TGATCA 1 cut(s) 1042
Kzo9I GATC 5 cut(s) 736, 1023, 1042, 1179, 1387
LmnI GCTCC 3 cut(s) 12, 133, 1163
Lsp1109I GCAGC 4 cut(s) 838, 901, 1000, 1167
LweI GCATC 4 cut(s) 1091, 1099, 1315, 1360
MaeI CTAG 2 cut(s) 120, 720
MaeII ACGT 2 cut(s) 45, 286
MaeIII GTNAC 5 cut(s) 299, 431, 543, 966, 1076
MalI GATC 5 cut(s) 738, 1025, 1044, 1181, 1389
MboI GATC 5 cut(s) 736, 1023, 1042, 1179, 1387
MboII GAAGA 8 cut(s) 31, 260, 451, 630, 831, 1303, 1369, 1436
MfeI CAATTG 1 cut(s) 1453
MflI RGATCY 2 cut(s) 736, 1387
MhlI GDGCHC 2 cut(s) 138, 755
MlsI TGGCCA 1 cut(s) 1053
MluCI AATT 6 cut(s) 95, 900, 1010, 1090, 1436, 1453
MluNI TGGCCA 1 cut(s) 1053
Mly113I GGCGCC 1 cut(s) 1293
MlyI GAGTC 2 cut(s) 563, 833
Mox20I TGGCCA 1 cut(s) 1053
Mph1103I ATGCAT 1 cut(s) 1114
MscI TGGCCA 1 cut(s) 1053
MslI CAYNNNNRTG 2 cut(s) 440, 975
Msp20I TGGCCA 1 cut(s) 1053
MspA1I CMGCKG 1 cut(s) 412
MspI CCGG 3 cut(s) 22, 72, 1149
MspR9I CCNGG 1 cut(s) 72
MunI CAATTG 1 cut(s) 1453
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 7 cut(s) 133, 216, 230, 452, 475, 759, 1259
NarI GGCGCC 1 cut(s) 1293
NciI CCSGG 1 cut(s) 72
NcoI CCATGG 1 cut(s) 801
NdeI CATATG 1 cut(s) 1005
NdeII GATC 5 cut(s) 736, 1023, 1042, 1179, 1387
NlaIII CATG 6 cut(s) 439, 751, 805, 947, 1049, 1116
NlaIV GGNNCC 5 cut(s) 60, 382, 753, 1294, 1389
NmeAIII GCCGAG 1 cut(s) 1321
NmuCI GTSAC 1 cut(s) 299
NsiI ATGCAT 1 cut(s) 1114
NspI RCATGY 1 cut(s) 1116
NspV TTCGAA 1 cut(s) 505
PaeR7I CTCGAG 1 cut(s) 137
PceI AGGCCT 1 cut(s) 1171
PcsI WCGNNNNNNNCGW 1 cut(s) 59
PflFI GACNNNGTC 1 cut(s) 224
PinAI ACCGGT 1 cut(s) 21
PkrI GCNGC 5 cut(s) 235, 853, 891, 990, 1157
PleI GAGTC 2 cut(s) 562, 832
PluTI GGCGCC 1 cut(s) 1296
PpsI GAGTC 2 cut(s) 562, 832
Psp124BI GAGCTC 1 cut(s) 138
PspN4I GGNNCC 5 cut(s) 60, 382, 753, 1294, 1389
PspOMI GGGCCC 1 cut(s) 751
PspPI GGNCC 3 cut(s) 19, 751, 752
PsuI RGATCY 2 cut(s) 736, 1387
PsyI GACNNNGTC 1 cut(s) 224
RsaI GTAC 1 cut(s) 382
RsaNI GTAC 1 cut(s) 381
RseI CAYNNNNRTG 2 cut(s) 440, 975
SacI GAGCTC 1 cut(s) 138
SatI GCNGC 5 cut(s) 234, 852, 890, 989, 1156
Sau3AI GATC 5 cut(s) 736, 1023, 1042, 1179, 1387
Sau96I GGNCC 3 cut(s) 19, 751, 752
SchI GAGTC 2 cut(s) 563, 833
ScrFI CCNGG 1 cut(s) 72
SduI GDGCHC 2 cut(s) 138, 755
SfaNI GCATC 4 cut(s) 1091, 1099, 1315, 1360
SfcI CTRYAG 3 cut(s) 997, 1173, 1209
SfoI GGCGCC 1 cut(s) 1294
Sfr274I CTCGAG 1 cut(s) 137
SfuI TTCGAA 1 cut(s) 505
SinI GGWCC 1 cut(s) 19
SlaI CTCGAG 1 cut(s) 137
SmiMI CAYNNNNRTG 2 cut(s) 440, 975
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 6 cut(s) 95, 900, 1010, 1090, 1436, 1453
SseBI AGGCCT 1 cut(s) 1171
SsiI CCGC 7 cut(s) 165, 177, 217, 234, 412, 1015, 1355
SspDI GGCGCC 1 cut(s) 1292
SspI AATATT 1 cut(s) 1069
SspMI CTAG 2 cut(s) 120, 720
SstI GAGCTC 1 cut(s) 138
StuI AGGCCT 1 cut(s) 1171
StyD4I CCNGG 1 cut(s) 70
StyI CCWWGG 3 cut(s) 119, 801, 1383
TaaI ACNGT 3 cut(s) 58, 541, 1213
TaiI ACGT 2 cut(s) 48, 289
TaqI TCGA 8 cut(s) 42, 104, 138, 160, 183, 505, 557, 730
TasI AATT 6 cut(s) 95, 900, 1010, 1090, 1436, 1453
TauI GCSGC 1 cut(s) 236
TscAI CASTG 1 cut(s) 1218
TseFI GTSAC 1 cut(s) 299
TseI GCWGC 4 cut(s) 851, 889, 988, 1155
Tsp45I GTSAC 1 cut(s) 299
TspDTI ATGAA 8 cut(s) 452, 524, 780, 788, 912, 1022, 1044, 1436
TspGWI ACGGA 3 cut(s) 184, 188, 1380
TspRI CASTG 1 cut(s) 1218
Tth111I GACNNNGTC 1 cut(s) 224
VpaK11BI GGWCC 1 cut(s) 19
XapI RAATTY 3 cut(s) 95, 900, 1090
XceI RCATGY 1 cut(s) 1116
XcmI CCANNNNNNNNNTGG 2 cut(s) 391, 874
XhoI CTCGAG 1 cut(s) 137
XmaJI CCTAGG 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 267
XspI CTAG 2 cut(s) 120, 720
Zsp2I ATGCAT 1 cut(s) 1114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.