AT5G07710
ERF Family

Polynucleotidyl transferase, ribonuclease H-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004362
Physical Location & Seq
Forward (+)
2453025 .. 2455546
2522 bp
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UTR
Exon/CDS
Intron
AT5G07710.1

Sequence Viewer

Length: 1407 bp
ATGGATCCAGAGGATAGGTCCGAGATTGCCTTCTTCGACGTTGAGACGACTGTACCAAAACGAGGTCAACGTTTCGCGATTCTAGAATTCGGGTCAATCCTCGTTTGCCCGAAGAAGCTAACGGAGCTCCGAAGCTACACGACTCTCGTCCAACCTGCAGATTTGAGTCTCATATCTTCCTTGTCCGTGAGGTGCAATGGTATCAAACGTGACGACGTCGTTTTGGCACCTCTGTTTGCCGACATCGCTGATACTGTCTACGACATTCTCCATGGGAGGATTTGGGCAGGTCATAACATATTGAGGTTTGATTGTGCAAGGATCAGAGAAGCATTTGCAGAGATTGGTCGTCAACCACCCGAGCCAAAAGGCGCCATTGATTCATTGGGTTTGCTAACTCAGAAGTTTGGTAGAAGAGCTGGTGATATGAAGATGGCAACTTTGGCTAGATATTTCGGACTCGGGAATCAGACGCATAGGAGCCTGGATGATGTTCGAATGAATCTTGAGGTTCTCAAATATTGTGCTACTGTCTTGTTCTTGGAGTCGAGTCTCCCATATGCACATGTAGATAATTCGGTATCTCCTGAAACTATTAGTTCAAGGAGACGTATTGATGCATCTCGCGAGGGTAATACTGTAACGACCTCTGTTAGGCTACCATCCATTAGCGAGAACAGTGCAGCTCAGCCAGATCCGTTCAACATGAGCGTCTTGAGGAATGAAATGGCTTCTGACAACCATATTCAATCAGACATTCTCATGGAAGAAGAACAAATTGAACCCTCTGATGTTGTCGCCTCTGAGAATACCAGTGACCATGAGGGTTTCTTGACGCCTGACGCGATGTCTCTTTCAAACATCAAAGCGATGCTCTTCCCGTTTTATCCTGGAAGCCAAATGATGAAACTAAAGCTACTCCACGGCGATTCCCCTCTGCAGCTCTACTGTTCTTATCTGAAAATACGGTTTGGAGTCAATGGGAAGTTTCTGGATAACACCGGGAGACGGAGGTTAAACTTTGTTGTTGATCTGAATCCAAGCCTTTACAGTATACTCGAAGCATGTGACAGTAATGCGCAGAAACTATCTGTTGATTCAGGTTCCACTTCTGAGTGGAACCCGGTCGTGAATCCAATGAAGGGATTTGTTAACTACCCTAACGCAAGGATACATATTGCGACAGAGATAAATGGAGATGAAGCTCGGTATGCAACAGAGATACACCAAAGAGAGTCCTCTGGAGCTACTCAGAAACTAATCTTCAGCAACCCGAACAATGAGGAGCTCGAATCGTTGCTGACCACGGGAAGTGTTGTTGATGCATTCTTGTCTCTTGAACCGTATGATTATCAGCAGAAAGCTGGAATCCGCCTTGTTGCCAAAAAGCTAGTCATTCAGTCGTAA

Protein Analysis

468

Amino Acids

52.17

Weight (kDa)

5.43

Isoelectric Point (pI)

54.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 11 - 172 1.3e-25 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 219
Acc16I TGCGCA 1 cut(s) 1080
Acc36I ACCTGC 2 cut(s) 163, 278
AccB1I GGYRCC 2 cut(s) 226, 371
AccI GTMKAC 2 cut(s) 258, 1054
AccII CGCG 3 cut(s) 77, 627, 845
AciI CCGC 1 cut(s) 1372
AclI AACGTT 1 cut(s) 70
AclWI GGATC 3 cut(s) 12, 329, 689
AcsI RAATTY 1 cut(s) 86
AcuI CTGAAG 1 cut(s) 1249
AcyI GRCGYC 3 cut(s) 216, 372, 836
AfaI GTAC 1 cut(s) 54
AfiI CCNNNNNNNGG 4 cut(s) 62, 654, 1008, 1142
AflIII ACRYGT 1 cut(s) 565
AgsI TTSAA 6 cut(s) 603, 703, 749, 782, 858, 1340
AhdI GACNNNNNGTC 1 cut(s) 847
AjnI CCWGG 2 cut(s) 483, 889
Alw21I GWGCWC 2 cut(s) 129, 1290
Alw26I GTCTC 7 cut(s) 38, 173, 557, 601, 855, 1000, 1338
AlwI GGATC 3 cut(s) 12, 329, 689
Ama87I CYCGRG 2 cut(s) 359, 461
ApeKI GCWGC 2 cut(s) 683, 940
ApoI RAATTY 1 cut(s) 86
ArsI GACNNNNNNTTYG 1 cut(s) 1377
AspLEI GCGC 2 cut(s) 374, 1081
AspS9I GGNCC 1 cut(s) 18
AsuC2I CCSGG 2 cut(s) 1003, 1124
AsuHPI GGTGA 1 cut(s) 434
AsuII TTCGAA 1 cut(s) 496
AvaI CYCGRG 2 cut(s) 359, 461
AvaII GGWCC 1 cut(s) 18
BaeI ACNNNNGTAYC 2 cut(s) 36, 69
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 2 cut(s) 226, 371
BanII GRGCYC 2 cut(s) 129, 1290
Bbv12I GWGCWC 2 cut(s) 129, 1290
BbvI GCAGC 2 cut(s) 695, 952
BccI CCATC 2 cut(s) 427, 670
BceAI ACGGC 1 cut(s) 940
BcgI CGANNNNNNTGC 2 cut(s) 662, 696
BciT130I CCWGG 2 cut(s) 485, 891
BciVI GTATCC 1 cut(s) 1164
BcnI CCSGG 2 cut(s) 1003, 1124
BcoDI GTCTC 7 cut(s) 38, 173, 557, 601, 855, 1000, 1338
BfaI CTAG 3 cut(s) 83, 447, 1391
BfmI CTRYAG 2 cut(s) 156, 938
BfoI RGCGCY 1 cut(s) 375
BfuAI ACCTGC 2 cut(s) 163, 278
BfuI GTATCC 1 cut(s) 1164
BisI GCNGC 2 cut(s) 684, 941
BlpI GCTNAGC 1 cut(s) 687
BlsI GCNGC 2 cut(s) 685, 942
Bme1390I CCNGG 4 cut(s) 485, 891, 1003, 1124
Bme18I GGWCC 1 cut(s) 18
BmeRI GACNNNNNGTC 1 cut(s) 847
BmeT110I CYCGRG 2 cut(s) 359, 461
BmgT120I GGNCC 1 cut(s) 18
BmiI GGNNCC 6 cut(s) 6, 228, 373, 482, 1105, 1121
BmrFI CCNGG 4 cut(s) 485, 891, 1003, 1124
BmsI GCATC 4 cut(s) 607, 629, 861, 1312
BoxI GACNNNNGTC 1 cut(s) 146
BpmI CTGGAG 1 cut(s) 1263
Bpu1102I GCTNAGC 1 cut(s) 687
Bpu14I TTCGAA 1 cut(s) 496
BpuEI CTTGAG 2 cut(s) 527, 736
BpuMI CCSGG 2 cut(s) 1003, 1124
BsaBI GATNNNNATC 1 cut(s) 1035
BsaHI GRCGYC 3 cut(s) 216, 372, 836
BsaJI CCNNGG 3 cut(s) 271, 922, 1305
Bsc4I CCNNNNNNNGG 4 cut(s) 62, 654, 1008, 1142
Bse1I ACTGG 1 cut(s) 813
Bse3DI GCAATG 1 cut(s) 202
Bse8I GATNNNNATC 1 cut(s) 1035
BseBI CCWGG 2 cut(s) 485, 891
BseDI CCNNGG 3 cut(s) 271, 922, 1305
BseGI GGATG 2 cut(s) 493, 662
BseJI GATNNNNATC 1 cut(s) 1035
BseLI CCNNNNNNNGG 4 cut(s) 62, 654, 1008, 1142
BseMI GCAATG 1 cut(s) 202
BseMII CTCAG 5 cut(s) 413, 701, 795, 1104, 1265
BseNI ACTGG 1 cut(s) 813
BseRI GAGGAG 1 cut(s) 1298
BseXI GCAGC 2 cut(s) 695, 952
BsgI GTGCAG 1 cut(s) 702
Bsh1236I CGCG 3 cut(s) 77, 627, 845
Bsh1285I CGRYCG 1 cut(s) 1128
BshNI GGYRCC 2 cut(s) 226, 371
BsiEI CGRYCG 1 cut(s) 1128
BsiHKAI GWGCWC 2 cut(s) 129, 1290
BsiHKCI CYCGRG 2 cut(s) 359, 461
BsiSI CCGG 2 cut(s) 1002, 1124
BslI CCNNNNNNNGG 4 cut(s) 62, 654, 1008, 1142
BsmAI GTCTC 7 cut(s) 38, 173, 557, 601, 855, 1000, 1338
BsmBI CGTCTC 3 cut(s) 38, 601, 1000
BsmI GAATGC 1 cut(s) 1325
BsoBI CYCGRG 2 cut(s) 359, 461
Bsp119I TTCGAA 1 cut(s) 496
Bsp1286I GDGCHC 2 cut(s) 129, 1290
Bsp143I GATC 4 cut(s) 4, 321, 694, 1030
Bsp1720I GCTNAGC 1 cut(s) 687
Bsp19I CCATGG 1 cut(s) 271
Bsp68I TCGCGA 2 cut(s) 77, 627
BspACI CCGC 1 cut(s) 1372
BspCNI CTCAG 5 cut(s) 412, 700, 796, 1105, 1264
BspFNI CGCG 3 cut(s) 77, 627, 845
BspLI GGNNCC 6 cut(s) 6, 228, 373, 482, 1105, 1121
BspMAI CTGCAG 2 cut(s) 160, 942
BspMI ACCTGC 2 cut(s) 163, 278
BspPI GGATC 3 cut(s) 12, 329, 689
BspQI GCTCTTC 2 cut(s) 409, 881
BspT104I TTCGAA 1 cut(s) 496
BspT107I GGYRCC 2 cut(s) 226, 371
BsrDI GCAATG 1 cut(s) 202
BsrI ACTGG 1 cut(s) 813
BssECI CCNNGG 3 cut(s) 271, 922, 1305
BssMI GATC 4 cut(s) 4, 321, 694, 1030
BssNAI GTATAC 1 cut(s) 1055
BssNI GRCGYC 3 cut(s) 216, 372, 836
BssT1I CCWWGG 1 cut(s) 271
Bst1107I GTATAC 1 cut(s) 1055
Bst2UI CCWGG 2 cut(s) 485, 891
Bst6I CTCTTC 2 cut(s) 409, 881
BstACI GRCGYC 3 cut(s) 216, 372, 836
BstBI TTCGAA 1 cut(s) 496
BstDEI CTNAG 5 cut(s) 399, 687, 804, 1113, 1251
BstDSI CCRYGG 3 cut(s) 271, 922, 1305
BstENI CCTNNNNNAGG 1 cut(s) 652
BstF5I GGATG 2 cut(s) 493, 662
BstFNI CGCG 3 cut(s) 77, 627, 845
BstH2I RGCGCY 1 cut(s) 375
BstHHI GCGC 2 cut(s) 374, 1081
BstKTI GATC 4 cut(s) 7, 324, 697, 1033
BstMAI GTCTC 7 cut(s) 38, 173, 557, 601, 855, 1000, 1338
BstMBI GATC 4 cut(s) 4, 321, 694, 1030
BstMCI CGRYCG 1 cut(s) 1128
BstMWI GCNNNNNNNGC 4 cut(s) 124, 245, 443, 1211
BstNI CCWGG 2 cut(s) 485, 891
BstNSI RCATGY 2 cut(s) 569, 1068
BstPAI GACNNNNGTC 1 cut(s) 146
BstSCI CCNGG 4 cut(s) 483, 889, 1001, 1122
BstSFI CTRYAG 2 cut(s) 156, 938
BstUI CGCG 3 cut(s) 77, 627, 845
BstV1I GCAGC 2 cut(s) 695, 952
BstX2I RGATCY 2 cut(s) 4, 694
BstYI RGATCY 2 cut(s) 4, 694
BstZ17I GTATAC 1 cut(s) 1055
BsuI GTATCC 1 cut(s) 1164
BtgI CCRYGG 3 cut(s) 271, 922, 1305
BtgZI GCGATG 3 cut(s) 229, 860, 884
BtsCI GGATG 2 cut(s) 493, 662
BtsIMutI CAGTG 2 cut(s) 685, 820
BtuMI TCGCGA 2 cut(s) 77, 627
BveI ACCTGC 2 cut(s) 163, 278
CfoI GCGC 2 cut(s) 374, 1081
Cfr13I GGNCC 1 cut(s) 18
CseI GACGC 4 cut(s) 481, 700, 844, 851
Csp6I GTAC 1 cut(s) 53
CviAII CATG 6 cut(s) 272, 566, 706, 763, 821, 1065
CviQI GTAC 1 cut(s) 53
DdeI CTNAG 5 cut(s) 399, 687, 804, 1113, 1251
DinI GGCGCC 1 cut(s) 373
DpnI GATC 4 cut(s) 6, 323, 696, 1032
DpnII GATC 4 cut(s) 4, 321, 694, 1030
DriI GACNNNNNGTC 1 cut(s) 847
Eam1104I CTCTTC 2 cut(s) 409, 881
Eam1105I GACNNNNNGTC 1 cut(s) 847
EarI CTCTTC 2 cut(s) 409, 881
EciI GGCGGA 1 cut(s) 1361
Ecl136II GAGCTC 2 cut(s) 127, 1288
Eco130I CCWWGG 1 cut(s) 271
Eco24I GRGCYC 2 cut(s) 129, 1290
Eco47I GGWCC 1 cut(s) 18
Eco53kI GAGCTC 2 cut(s) 127, 1288
Eco57I CTGAAG 1 cut(s) 1249
Eco88I CYCGRG 2 cut(s) 359, 461
EcoICRI GAGCTC 2 cut(s) 127, 1288
EcoNI CCTNNNNNAGG 1 cut(s) 652
EcoRI GAATTC 1 cut(s) 86
EcoRII CCWGG 2 cut(s) 483, 889
EcoT14I CCWWGG 1 cut(s) 271
EcoT22I ATGCAT 2 cut(s) 622, 1327
EcoT38I GRGCYC 2 cut(s) 129, 1290
EgeI GGCGCC 1 cut(s) 373
EheI GGCGCC 1 cut(s) 373
ErhI CCWWGG 1 cut(s) 271
Esp3I CGTCTC 3 cut(s) 38, 601, 1000
FaeI CATG 6 cut(s) 275, 569, 709, 766, 824, 1068
FatI CATG 6 cut(s) 271, 565, 705, 762, 820, 1064
FauNDI CATATG 1 cut(s) 559
FblI GTMKAC 2 cut(s) 258, 1054
Fnu4HI GCNGC 2 cut(s) 684, 941
FokI GGATG 2 cut(s) 500, 649
FriOI GRGCYC 2 cut(s) 129, 1290
Fsp4HI GCNGC 2 cut(s) 684, 941
FspBI CTAG 3 cut(s) 83, 447, 1391
FspI TGCGCA 1 cut(s) 1080
GlaI GCGC 2 cut(s) 373, 1080
GluI GCNGC 2 cut(s) 684, 941
GsuI CTGGAG 1 cut(s) 1263
HaeII RGCGCY 1 cut(s) 375
HapII CCGG 2 cut(s) 1002, 1124
HgaI GACGC 4 cut(s) 481, 700, 844, 851
HhaI GCGC 2 cut(s) 374, 1081
Hin1I GRCGYC 3 cut(s) 216, 372, 836
Hin1II CATG 6 cut(s) 275, 569, 709, 766, 824, 1068
Hin6I GCGC 2 cut(s) 372, 1079
HinP1I GCGC 2 cut(s) 372, 1079
HincII GTYRAC 3 cut(s) 68, 353, 1153
HindII GTYRAC 3 cut(s) 68, 353, 1153
HpaI GTTAAC 1 cut(s) 1153
HpaII CCGG 2 cut(s) 1002, 1124
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 5 cut(s) 68, 259, 353, 1055, 1153
Hpy8I GTNNAC 5 cut(s) 68, 259, 353, 1055, 1153
Hpy99I CGWCG 3 cut(s) 41, 218, 221
HpyAV CCTTC 2 cut(s) 40, 1135
HpyCH4IV ACGT 5 cut(s) 39, 70, 208, 216, 610
HpyF10VI GCNNNNNNNGC 4 cut(s) 124, 245, 443, 1211
HpyF3I CTNAG 5 cut(s) 399, 687, 804, 1113, 1251
HpySE526I ACGT 5 cut(s) 39, 70, 208, 216, 610
Hsp92I GRCGYC 3 cut(s) 216, 372, 836
Hsp92II CATG 6 cut(s) 275, 569, 709, 766, 824, 1068
HspAI GCGC 2 cut(s) 372, 1079
KasI GGCGCC 1 cut(s) 371
KspAI GTTAAC 1 cut(s) 1153
Kzo9I GATC 4 cut(s) 4, 321, 694, 1030
LguI GCTCTTC 2 cut(s) 409, 881
LmnI GCTCC 5 cut(s) 124, 132, 480, 1244, 1285
Lsp1109I GCAGC 2 cut(s) 695, 952
LweI GCATC 4 cut(s) 607, 629, 861, 1312
MaeI CTAG 3 cut(s) 83, 447, 1391
MaeII ACGT 5 cut(s) 39, 70, 208, 216, 610
MaeIII GTNAC 4 cut(s) 209, 640, 815, 1067
MalI GATC 4 cut(s) 6, 323, 696, 1032
MboI GATC 4 cut(s) 4, 321, 694, 1030
MboII GAAGA 9 cut(s) 25, 124, 168, 426, 442, 779, 782, 868, 1255
MflI RGATCY 2 cut(s) 4, 694
MhlI GDGCHC 2 cut(s) 129, 1290
MluCI AATT 3 cut(s) 86, 574, 777
Mly113I GGCGCC 1 cut(s) 372
MlyI GAGTC 7 cut(s) 136, 175, 453, 554, 559, 984, 1244
MmeI TCCRAC 1 cut(s) 175
Mph1103I ATGCAT 2 cut(s) 622, 1327
MseI TTAA 2 cut(s) 1016, 1152
MslI CAYNNNNRTG 1 cut(s) 761
MspI CCGG 2 cut(s) 1002, 1124
MspR9I CCNGG 4 cut(s) 485, 891, 1003, 1124
Mva1269I GAATGC 1 cut(s) 1325
MvaI CCWGG 2 cut(s) 485, 891
MvnI CGCG 3 cut(s) 77, 627, 845
MwoI GCNNNNNNNGC 4 cut(s) 124, 245, 443, 1211
NarI GGCGCC 1 cut(s) 372
NciI CCSGG 2 cut(s) 1003, 1124
NcoI CCATGG 1 cut(s) 271
NdeI CATATG 1 cut(s) 559
NdeII GATC 4 cut(s) 4, 321, 694, 1030
NlaIII CATG 6 cut(s) 275, 569, 709, 766, 824, 1068
NlaIV GGNNCC 6 cut(s) 6, 228, 373, 482, 1105, 1121
NmuCI GTSAC 3 cut(s) 209, 815, 1067
NruI TCGCGA 2 cut(s) 77, 627
NsbI TGCGCA 1 cut(s) 1080
NsiI ATGCAT 2 cut(s) 622, 1327
NspI RCATGY 2 cut(s) 569, 1068
NspV TTCGAA 1 cut(s) 496
PciI ACATGT 1 cut(s) 565
PciSI GCTCTTC 2 cut(s) 409, 881
PcsI WCGNNNNNNNCGW 2 cut(s) 67, 842
PctI GAATGC 1 cut(s) 1325
PflFI GACNNNGTC 1 cut(s) 215
PfoI TCCNGGA 1 cut(s) 889
PkrI GCNGC 2 cut(s) 685, 942
PleI GAGTC 7 cut(s) 136, 174, 453, 553, 558, 983, 1243
PluTI GGCGCC 1 cut(s) 375
PpsI GAGTC 7 cut(s) 136, 174, 453, 553, 558, 983, 1243
PscI ACATGT 1 cut(s) 565
PshAI GACNNNNGTC 1 cut(s) 146
Psp124BI GAGCTC 2 cut(s) 129, 1290
Psp1406I AACGTT 1 cut(s) 70
Psp6I CCWGG 2 cut(s) 483, 889
PspGI CCWGG 2 cut(s) 483, 889
PspN4I GGNNCC 6 cut(s) 6, 228, 373, 482, 1105, 1121
PspPI GGNCC 1 cut(s) 18
PstI CTGCAG 2 cut(s) 160, 942
PsuI RGATCY 2 cut(s) 4, 694
PsyI GACNNNGTC 1 cut(s) 215
RruI TCGCGA 2 cut(s) 77, 627
RsaI GTAC 1 cut(s) 54
RsaNI GTAC 1 cut(s) 53
RseI CAYNNNNRTG 1 cut(s) 761
SacI GAGCTC 2 cut(s) 129, 1290
SapI GCTCTTC 2 cut(s) 409, 881
SaqAI TTAA 2 cut(s) 1016, 1152
SatI GCNGC 2 cut(s) 684, 941
Sau3AI GATC 4 cut(s) 4, 321, 694, 1030
Sau96I GGNCC 1 cut(s) 18
SchI GAGTC 7 cut(s) 136, 175, 453, 554, 559, 984, 1244
ScrFI CCNGG 4 cut(s) 485, 891, 1003, 1124
SduI GDGCHC 2 cut(s) 129, 1290
SfaNI GCATC 4 cut(s) 607, 629, 861, 1312
SfcI CTRYAG 2 cut(s) 156, 938
SfoI GGCGCC 1 cut(s) 373
SfuI TTCGAA 1 cut(s) 496
SinI GGWCC 1 cut(s) 18
SmiMI CAYNNNNRTG 1 cut(s) 761
SmlI CTYRAG 2 cut(s) 506, 715
SmoI CTYRAG 2 cut(s) 506, 715
Sse9I AATT 3 cut(s) 86, 574, 777
SsiI CCGC 1 cut(s) 1372
SspDI GGCGCC 1 cut(s) 371
SspI AATATT 1 cut(s) 521
SspMI CTAG 3 cut(s) 83, 447, 1391
SstI GAGCTC 2 cut(s) 129, 1290
StyD4I CCNGG 4 cut(s) 483, 889, 1001, 1122
StyI CCWWGG 1 cut(s) 271
TaiI ACGT 5 cut(s) 42, 73, 211, 219, 613
TaqI TCGA 5 cut(s) 36, 496, 548, 1059, 1290
TasI AATT 3 cut(s) 86, 574, 777
Tru1I TTAA 2 cut(s) 1016, 1152
Tru9I TTAA 2 cut(s) 1016, 1152
TscAI CASTG 2 cut(s) 685, 820
TseFI GTSAC 3 cut(s) 209, 815, 1067
TseI GCWGC 2 cut(s) 683, 940
Tsp45I GTSAC 3 cut(s) 209, 815, 1067
TspDTI ATGAA 7 cut(s) 372, 443, 515, 738, 920, 1154, 1215
TspGWI ACGGA 4 cut(s) 137, 175, 687, 1024
TspRI CASTG 2 cut(s) 685, 820
Tth111I GACNNNGTC 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 18
XagI CCTNNNNNAGG 1 cut(s) 652
XapI RAATTY 1 cut(s) 86
XbaI TCTAGA 1 cut(s) 82
XceI RCATGY 2 cut(s) 569, 1068
XcmI CCANNNNNNNNNTGG 1 cut(s) 382
XmiI GTMKAC 2 cut(s) 258, 1054
XspI CTAG 3 cut(s) 83, 447, 1391
ZraI GACGTC 1 cut(s) 217
Zsp2I ATGCAT 2 cut(s) 622, 1327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.