FvH4_5g14590

EXOIII

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004362
Physical Location & Seq
Reverse (-)
8279944 .. 8282862
2919 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14590.t1

Sequence Viewer

Length: 1500 bp
ATGACGAATCAGGGCGGCGAGGAGAGGTTCGAGATCGCCTTCTTCGACGTGGAGACGACGGTGCCGACCCGACCCGGTCAGGGCTTCGCTTTATTGGAATTCGGATCCATCCTTGTTTGCCCCAGGAAGCTGATTGAGCTCCAGAGCTACTCCACTCTCATACGACCCGCCGACCTCAATTCCATCAATTCCTTGTCGGTGCGCTGTAACGGCATTACCAGAGACGCCGTTATAGCCGCGCCGAGTTTCGATGAGATTGCCGATAGAGTCTTCGATATACTCCATGGGCGTATATGGGCTGGTCATAACATATTGAGGTTTGATTGTCCCCGTATTCGGGAGGCTTTTGCAAGTATTGGTCGGCCTGCACCAGAACCCAAAGGTACAATTGATTCTTTGGCTTTGTTGACTCAGCGATTTGGAAGGAGAGCTGGTAACATGAAGATGGCCAGTCTTGCAACATATTTTGGTCTTGGACAACAGACACACAGGAGTTTGGATGATGTTCGGATGAATCTTGAAGTACTCAAATATTGTGCTACGGTTTTATTCTTGGAGTCAAGCCTCCCGGACATATTAACAGAGAACAGTTGGGTTTCTCCAAATGCTACAACAAGAAGCCGAAGTGGTGGAAACTTATCTCCTGAGGGTTCGAGCATGAACAACAATATTTCAAACCAAACAGGGGACAATCATCCAATAACATCCCTTAGAACTGAGACCACACAAGAAGTCTCTGATTTGCTTGAGACTGTTGAGTCCAGCACAAATCGACCTGATCCTTTGGACATGGGCGCATTTATCGATGAGGTGAAGAAAGAGTTAGATCAGCAAGACATGAACATGGTAGAAACACCTGCACAAGAGTCTCCCAATAGTTCTGTCAATGCTGCTGTGACCAACAACTGTAATGCCACCATGGAATTTTTACAGCTCAATCAAGTTTTCATTCCTTCTATCAGTGCTTCGCTTGTTCCACTGAATCGTGGGATTCAAAGGATAAAGTTATTACACAAAGATGCCACTTTGCAGCTTTGCTGCAGTCATATGAAATTGCGGTTTGGAATCAATGCGAAGTTTTTTGATCACGCTGGCCGACCACGGCTGAACATTGTCTTTGATGCACCACCAAAATTATGTGAAGTTCTTGATGCATGTGATGGCCTTGCACAGAAGTTGTCTTTGGATTCTGGCAGTAGCTCTGAATGGAGGCCTGTTGTGATCCGCAAAGAGGGCTACTACAACTACCCTACTGTGAGATTGCACATACAAACTGCAGTATGCGGGGACATTGCGATCTACGCCACAGAGATATATCAAAGAGAGCCCTCTGGCACTGATCAAAGACTAGTCTTCTCCAAATTTGATGCTTCAGAACTTAGTGCCTTGTTCACCCGAGGGATGTTTATGGATGTGTTCTTCTCTTTGGATCCATATGACTATCAGCAGAGTGCAGGCATTAGGTTGGTGGCAAAGAAACTGATCATTCATTCCAATTGA

Protein Analysis

500

Amino Acids

55.44

Weight (kDa)

5.6

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 14 - 176 1.1e-25 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 865
AasI GACNNNNNNGTC 1 cut(s) 757
Acc36I ACCTGC 1 cut(s) 865
AccB1I GGYRCC 1 cut(s) 61
AccII CGCG 1 cut(s) 239
AciI CCGC 6 cut(s) 15, 168, 237, 1057, 1225, 1284
AclWI GGATC 6 cut(s) 99, 112, 773, 1216, 1424, 1437
AcoI YGGCCR 2 cut(s) 447, 1093
AcsI RAATTY 3 cut(s) 98, 923, 1361
AcuI CTGAAG 1 cut(s) 1356
AcyI GRCGYC 1 cut(s) 225
AfaI GTAC 2 cut(s) 385, 525
AfiI CCNNNNNNNGG 5 cut(s) 80, 336, 337, 685, 1231
AgsI TTSAA 3 cut(s) 521, 675, 995
AhlI ACTAGT 1 cut(s) 1348
AjiI CACGTC 1 cut(s) 49
AjnI CCWGG 1 cut(s) 122
AjuI GAANNNNNNNTTGG 2 cut(s) 1166, 1198
AluBI AGCT 7 cut(s) 130, 139, 147, 431, 934, 1033, 1200
AluI AGCT 7 cut(s) 130, 139, 147, 431, 934, 1033, 1200
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 6 cut(s) 47, 216, 713, 739, 743, 873
AlwI GGATC 6 cut(s) 99, 112, 773, 1216, 1424, 1437
Ama87I CYCGRG 1 cut(s) 1395
AoxI GGCC 5 cut(s) 362, 447, 1093, 1162, 1211
ApeKI GCWGC 3 cut(s) 890, 1030, 1038
ApoI RAATTY 3 cut(s) 98, 923, 1361
AspLEI GCGC 3 cut(s) 204, 241, 797
AsuC2I CCSGG 2 cut(s) 75, 569
AsuHPI GGTGA 2 cut(s) 823, 1384
AvaI CYCGRG 1 cut(s) 1395
AxyI CCTNAGG 1 cut(s) 645
BalI TGGCCA 1 cut(s) 449
BamHI GGATCC 2 cut(s) 104, 1429
BanI GGYRCC 1 cut(s) 61
BanII GRGCYC 2 cut(s) 141, 1329
BbsI GAAGAC 2 cut(s) 262, 1345
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 3 cut(s) 877, 1025, 1042
BccI CCATC 4 cut(s) 116, 191, 439, 1154
BceAI ACGGC 3 cut(s) 212, 226, 1118
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BciT130I CCWGG 1 cut(s) 124
BclI TGATCA 3 cut(s) 1084, 1339, 1482
BcnI CCSGG 2 cut(s) 75, 569
BcoDI GTCTC 6 cut(s) 47, 216, 713, 739, 743, 873
BcuI ACTAGT 1 cut(s) 1348
BfaI CTAG 1 cut(s) 1349
BfmI CTRYAG 2 cut(s) 1039, 1275
BfuAI ACCTGC 1 cut(s) 865
BisI GCNGC 5 cut(s) 16, 237, 891, 1031, 1039
BlsI GCNGC 5 cut(s) 17, 238, 892, 1032, 1040
BmcAI AGTACT 1 cut(s) 525
Bme1390I CCNGG 3 cut(s) 75, 124, 569
BmeT110I CYCGRG 1 cut(s) 1395
BmgBI CACGTC 1 cut(s) 49
BmiI GGNNCC 3 cut(s) 63, 106, 1431
BmrFI CCNGG 3 cut(s) 75, 124, 569
BmsI GCATC 4 cut(s) 1009, 1111, 1141, 1357
BpiI GAAGAC 2 cut(s) 262, 1345
BpmI CTGGAG 1 cut(s) 125
BpuEI CTTGAG 1 cut(s) 767
BpuMI CCSGG 2 cut(s) 75, 569
Bsa29I ATCGAT 1 cut(s) 804
BsaHI GRCGYC 1 cut(s) 225
BsaI GGTCTC 1 cut(s) 713
BsaJI CCNNGG 5 cut(s) 122, 283, 918, 1100, 1396
BsaXI ACNNNNNCTCC 2 cut(s) 418, 448
Bsc4I CCNNNNNNNGG 5 cut(s) 80, 336, 337, 685, 1231
Bse1I ACTGG 1 cut(s) 450
Bse21I CCTNAGG 1 cut(s) 645
Bse3DI GCAATG 1 cut(s) 1290
BseBI CCWGG 1 cut(s) 124
BseCI ATCGAT 1 cut(s) 804
BseDI CCNNGG 5 cut(s) 122, 283, 918, 1100, 1396
BseGI GGATG 7 cut(s) 108, 505, 516, 694, 704, 1407, 1417
BseLI CCNNNNNNNGG 5 cut(s) 80, 336, 337, 685, 1231
BseMI GCAATG 1 cut(s) 1290
BseMII CTCAG 3 cut(s) 425, 636, 708
BseNI ACTGG 1 cut(s) 450
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 3 cut(s) 877, 1025, 1042
BsgI GTGCAG 3 cut(s) 351, 843, 1473
Bsh1236I CGCG 1 cut(s) 239
BshFI GGCC 5 cut(s) 364, 449, 1095, 1164, 1213
BshNI GGYRCC 1 cut(s) 61
BshVI ATCGAT 1 cut(s) 804
BsiHKAI GWGCWC 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 1395
BsiSI CCGG 2 cut(s) 75, 569
BslFI GGGAC 3 cut(s) 312, 701, 1301
BslI CCNNNNNNNGG 5 cut(s) 80, 336, 337, 685, 1231
BsmAI GTCTC 6 cut(s) 47, 216, 713, 739, 743, 873
BsmBI CGTCTC 2 cut(s) 47, 216
BsmFI GGGAC 3 cut(s) 312, 701, 1301
BsnI GGCC 5 cut(s) 364, 449, 1095, 1164, 1213
Bso31I GGTCTC 1 cut(s) 713
BsoBI CYCGRG 1 cut(s) 1395
Bsp1286I GDGCHC 2 cut(s) 141, 1329
Bsp19I CCATGG 2 cut(s) 283, 918
BspACI CCGC 6 cut(s) 15, 168, 237, 1057, 1225, 1284
BspANI GGCC 5 cut(s) 364, 449, 1095, 1164, 1213
BspCNI CTCAG 3 cut(s) 424, 637, 709
BspDI ATCGAT 1 cut(s) 804
BspFNI CGCG 1 cut(s) 239
BspLI GGNNCC 3 cut(s) 63, 106, 1431
BspMAI CTGCAG 2 cut(s) 1043, 1279
BspMI ACCTGC 1 cut(s) 865
BspPI GGATC 6 cut(s) 99, 112, 773, 1216, 1424, 1437
BspT107I GGYRCC 1 cut(s) 61
BspTNI GGTCTC 1 cut(s) 713
BsrDI GCAATG 1 cut(s) 1290
BsrI ACTGG 1 cut(s) 450
BssECI CCNNGG 5 cut(s) 122, 283, 918, 1100, 1396
BssNI GRCGYC 1 cut(s) 225
BssT1I CCWWGG 2 cut(s) 283, 918
Bst2UI CCWGG 1 cut(s) 124
Bst4CI ACNGT 6 cut(s) 61, 544, 590, 754, 908, 1255
BstACI GRCGYC 1 cut(s) 225
BstC8I GCNNGC 3 cut(s) 366, 1093, 1456
BstDEI CTNAG 5 cut(s) 411, 645, 710, 717, 1379
BstDSI CCRYGG 3 cut(s) 283, 918, 1100
BstF5I GGATG 7 cut(s) 108, 505, 516, 694, 704, 1407, 1417
BstFNI CGCG 1 cut(s) 239
BstHHI GCGC 3 cut(s) 204, 241, 797
BstMAI GTCTC 6 cut(s) 47, 216, 713, 739, 743, 873
BstMWI GCNNNNNNNGC 6 cut(s) 136, 210, 233, 455, 1233, 1301
BstNI CCWGG 1 cut(s) 124
BstNSI RCATGY 1 cut(s) 1158
BstSCI CCNGG 3 cut(s) 73, 122, 567
BstSFI CTRYAG 2 cut(s) 1039, 1275
BstUI CGCG 1 cut(s) 239
BstV1I GCAGC 3 cut(s) 877, 1025, 1042
BstV2I GAAGAC 2 cut(s) 262, 1345
BstX2I RGATCY 2 cut(s) 104, 1429
BstYI RGATCY 2 cut(s) 104, 1429
Bsu15I ATCGAT 1 cut(s) 804
Bsu36I CCTNAGG 1 cut(s) 645
BsuRI GGCC 5 cut(s) 364, 449, 1095, 1164, 1213
BsuTUI ATCGAT 1 cut(s) 804
BtgI CCRYGG 3 cut(s) 283, 918, 1100
BtrI CACGTC 1 cut(s) 49
BtsCI GGATG 7 cut(s) 108, 505, 516, 694, 704, 1407, 1417
BtsIMutI CAGTG 3 cut(s) 967, 977, 1335
BveI ACCTGC 1 cut(s) 865
Cac8I GCNNGC 3 cut(s) 366, 1093, 1456
CfoI GCGC 3 cut(s) 204, 241, 797
ClaI ATCGAT 1 cut(s) 804
CseI GACGC 1 cut(s) 233
Csp6I GTAC 2 cut(s) 384, 524
CviAII CATG 8 cut(s) 284, 439, 658, 790, 838, 844, 919, 1155
CviQI GTAC 2 cut(s) 384, 524
DdeI CTNAG 5 cut(s) 411, 645, 710, 717, 1379
DrdI GACNNNNNNGTC 1 cut(s) 757
DseDI GACNNNNNNGTC 1 cut(s) 757
EaeI YGGCCR 2 cut(s) 447, 1093
Ecl136II GAGCTC 1 cut(s) 139
Eco130I CCWWGG 2 cut(s) 283, 918
Eco147I AGGCCT 1 cut(s) 1213
Eco24I GRGCYC 2 cut(s) 141, 1329
Eco31I GGTCTC 1 cut(s) 713
Eco53kI GAGCTC 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 1356
Eco81I CCTNAGG 1 cut(s) 645
Eco88I CYCGRG 1 cut(s) 1395
EcoICRI GAGCTC 1 cut(s) 139
EcoRI GAATTC 1 cut(s) 98
EcoRII CCWGG 1 cut(s) 122
EcoT14I CCWWGG 2 cut(s) 283, 918
EcoT22I ATGCAT 1 cut(s) 1156
EcoT38I GRGCYC 2 cut(s) 141, 1329
ErhI CCWWGG 2 cut(s) 283, 918
Esp3I CGTCTC 2 cut(s) 47, 216
FaeI CATG 8 cut(s) 287, 442, 661, 793, 841, 847, 922, 1158
FaqI GGGAC 3 cut(s) 312, 701, 1301
FatI CATG 8 cut(s) 283, 438, 657, 789, 837, 843, 918, 1154
FauI CCCGC 2 cut(s) 175, 1277
FauNDI CATATG 2 cut(s) 1047, 1435
FbaI TGATCA 3 cut(s) 1084, 1339, 1482
Fnu4HI GCNGC 5 cut(s) 16, 237, 891, 1031, 1039
FokI GGATG 7 cut(s) 95, 512, 523, 681, 691, 1414, 1424
FriOI GRGCYC 2 cut(s) 141, 1329
Fsp4HI GCNGC 5 cut(s) 16, 237, 891, 1031, 1039
FspBI CTAG 1 cut(s) 1349
GlaI GCGC 3 cut(s) 203, 240, 796
GluI GCNGC 5 cut(s) 16, 237, 891, 1031, 1039
GsuI CTGGAG 1 cut(s) 125
HaeIII GGCC 5 cut(s) 364, 449, 1095, 1164, 1213
HapII CCGG 2 cut(s) 75, 569
HgaI GACGC 1 cut(s) 233
HhaI GCGC 3 cut(s) 204, 241, 797
Hin1I GRCGYC 1 cut(s) 225
Hin1II CATG 8 cut(s) 287, 442, 661, 793, 841, 847, 922, 1158
Hin6I GCGC 3 cut(s) 202, 239, 795
HinP1I GCGC 3 cut(s) 202, 239, 795
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HpaII CCGG 2 cut(s) 75, 569
HphI GGTGA 2 cut(s) 823, 1384
Hpy166II GTNNAC 2 cut(s) 408, 1392
Hpy188I TCNGA 5 cut(s) 104, 510, 739, 1204, 1375
Hpy188III TCNNGA 6 cut(s) 31, 142, 338, 518, 644, 1148
Hpy8I GTNNAC 2 cut(s) 408, 1392
Hpy99I CGWCG 2 cut(s) 50, 61
HpyAV CCTTC 3 cut(s) 49, 417, 963
HpyCH4III ACNGT 6 cut(s) 61, 544, 590, 754, 908, 1255
HpyCH4IV ACGT 1 cut(s) 48
HpyF10VI GCNNNNNNNGC 6 cut(s) 136, 210, 233, 455, 1233, 1301
HpyF3I CTNAG 5 cut(s) 411, 645, 710, 717, 1379
HpySE526I ACGT 1 cut(s) 48
Hsp92I GRCGYC 1 cut(s) 225
Hsp92II CATG 8 cut(s) 287, 442, 661, 793, 841, 847, 922, 1158
HspAI GCGC 3 cut(s) 202, 239, 795
Ksp22I TGATCA 3 cut(s) 1084, 1339, 1482
LmnI GCTCC 1 cut(s) 144
Lsp1109I GCAGC 3 cut(s) 877, 1025, 1042
LweI GCATC 4 cut(s) 1009, 1111, 1141, 1357
MaeI CTAG 1 cut(s) 1349
MaeII ACGT 1 cut(s) 48
MaeIII GTNAC 3 cut(s) 206, 434, 895
MboII GAAGA 6 cut(s) 34, 262, 454, 826, 1345, 1411
MfeI CAATTG 2 cut(s) 387, 1495
MflI RGATCY 2 cut(s) 104, 1429
MhlI GDGCHC 2 cut(s) 141, 1329
MlsI TGGCCA 1 cut(s) 449
MluCI AATT 9 cut(s) 98, 178, 187, 387, 923, 1052, 1133, 1361, 1495
MluNI TGGCCA 1 cut(s) 449
MlyI GAGTC 5 cut(s) 276, 403, 566, 767, 875
Mox20I TGGCCA 1 cut(s) 449
Mph1103I ATGCAT 1 cut(s) 1156
MscI TGGCCA 1 cut(s) 449
MseI TTAA 1 cut(s) 578
MslI CAYNNNNRTG 3 cut(s) 443, 842, 1017
Msp20I TGGCCA 1 cut(s) 449
MspI CCGG 2 cut(s) 75, 569
MspR9I CCNGG 3 cut(s) 75, 124, 569
MunI CAATTG 2 cut(s) 387, 1495
MvaI CCWGG 1 cut(s) 124
MvnI CGCG 1 cut(s) 239
MwoI GCNNNNNNNGC 6 cut(s) 136, 210, 233, 455, 1233, 1301
NciI CCSGG 2 cut(s) 75, 569
NcoI CCATGG 2 cut(s) 283, 918
NdeI CATATG 2 cut(s) 1047, 1435
NlaIII CATG 8 cut(s) 287, 442, 661, 793, 841, 847, 922, 1158
NlaIV GGNNCC 3 cut(s) 63, 106, 1431
NmeAIII GCCGAG 1 cut(s) 267
NmuCI GTSAC 1 cut(s) 895
NsiI ATGCAT 1 cut(s) 1156
NspI RCATGY 1 cut(s) 1158
PaqCI CACCTGC 1 cut(s) 865
PceI AGGCCT 1 cut(s) 1213
PcsI WCGNNNNNNNCGW 2 cut(s) 42, 62
PfeI GAWTC 7 cut(s) 7, 392, 514, 982, 991, 1065, 1187
PflFI GACNNNGTC 1 cut(s) 75
PfoI TCCNGGA 1 cut(s) 567
PkrI GCNGC 5 cut(s) 17, 238, 892, 1032, 1040
PleI GAGTC 5 cut(s) 275, 403, 565, 766, 874
PpsI GAGTC 5 cut(s) 275, 403, 565, 766, 874
Psp124BI GAGCTC 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 122
PspGI CCWGG 1 cut(s) 122
PspN4I GGNNCC 3 cut(s) 63, 106, 1431
PstI CTGCAG 2 cut(s) 1043, 1279
PsuI RGATCY 2 cut(s) 104, 1429
PsyI GACNNNGTC 1 cut(s) 75
RsaI GTAC 2 cut(s) 385, 525
RsaNI GTAC 2 cut(s) 384, 524
RseI CAYNNNNRTG 3 cut(s) 443, 842, 1017
SacI GAGCTC 1 cut(s) 141
SaqAI TTAA 1 cut(s) 578
SatI GCNGC 5 cut(s) 16, 237, 891, 1031, 1039
ScaI AGTACT 1 cut(s) 525
SchI GAGTC 5 cut(s) 276, 403, 566, 767, 875
ScrFI CCNGG 3 cut(s) 75, 124, 569
SduI GDGCHC 2 cut(s) 141, 1329
SfaNI GCATC 4 cut(s) 1009, 1111, 1141, 1357
SfcI CTRYAG 2 cut(s) 1039, 1275
SmiMI CAYNNNNRTG 3 cut(s) 443, 842, 1017
SmlI CTYRAG 1 cut(s) 746
SmoI CTYRAG 1 cut(s) 746
SpeI ACTAGT 1 cut(s) 1348
Sse9I AATT 9 cut(s) 98, 178, 187, 387, 923, 1052, 1133, 1361, 1495
SseBI AGGCCT 1 cut(s) 1213
SsiI CCGC 6 cut(s) 15, 168, 237, 1057, 1225, 1284
SspI AATATT 2 cut(s) 533, 670
SspMI CTAG 1 cut(s) 1349
SstI GAGCTC 1 cut(s) 141
StuI AGGCCT 1 cut(s) 1213
StyD4I CCNGG 3 cut(s) 73, 122, 567
StyI CCWWGG 2 cut(s) 283, 918
TaaI ACNGT 6 cut(s) 61, 544, 590, 754, 908, 1255
TaiI ACGT 1 cut(s) 51
TaqI TCGA 7 cut(s) 30, 45, 249, 273, 653, 772, 804
TasI AATT 9 cut(s) 98, 178, 187, 387, 923, 1052, 1133, 1361, 1495
TatI WGTACW 1 cut(s) 523
TauI GCSGC 2 cut(s) 18, 239
TfiI GAWTC 7 cut(s) 7, 392, 514, 982, 991, 1065, 1187
Tru1I TTAA 1 cut(s) 578
Tru9I TTAA 1 cut(s) 578
TscAI CASTG 3 cut(s) 967, 984, 1342
TseFI GTSAC 1 cut(s) 895
TseI GCWGC 3 cut(s) 890, 1030, 1038
Tsp45I GTSAC 1 cut(s) 895
TspDTI ATGAA 7 cut(s) 455, 527, 674, 854, 937, 1064, 1478
TspRI CASTG 3 cut(s) 967, 984, 1342
Tth111I GACNNNGTC 1 cut(s) 75
XapI RAATTY 3 cut(s) 98, 923, 1361
XceI RCATGY 1 cut(s) 1158
XspI CTAG 1 cut(s) 1349
ZrmI AGTACT 1 cut(s) 525
Zsp2I ATGCAT 1 cut(s) 1156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.