MD14G1204400.v1.1

EXOIII

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004362
Physical Location & Seq
Forward (+)
29240725 .. 29243501
2777 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1204400.v1.1.491

Sequence Viewer

Length: 1521 bp
ATGGCGAGTCGGAGCGAGGATCGGTTCGAAATAGCCTTCTTCGACGTGGAGACGATGGTGCCGACCCGATCCGGACAGGGCTTCACCATCTTGGAATTCGGGTCGATTCTCGTTTGCCCTAGGAAGCTGGTGGAGCTCGAGAGCTACTCCACCCTGATTCGACCCGCCGACCTCTCCGCCATCAATTCCCTGTCCGTCCGTGGCAACGGCATTACCCGCGACGCCGTCATTGCCGCCCCTTCTTTTCAAGACATCGCCGATACGGTCTACGACATTCTCCACGAACGTATATGGGCTGGTCACAACATTCTGAGGTTTGATTGTTATCGGATTCGGGAGGCTTTCGCACGCATTGGTCGGCCTGCGCCAGAACCGAAAGGTACCATTGATTCGTTGGCATTGTTGACACAGCGGTTCGGAAGGAGAGCTGGTAACATGAAGATGGCCTCCCTTGCAACCTATTTCGGGATTGGACAGCAGACACACAGGAGTTTGGATGATGTTCGAATGAATCTTGAAGTTCTGAAGTATTGTGCAACCGTGTTACTCTTGGAGTCGAGCCTCCCAGACATATTCACAGAGAATAGTTGGGTTTCTCCAAATGCTACCACAAGAAGTCGTAGTGACGGGAAATCATCTGCTAAGGGGTCTCGCATAAACATGAATACTGAGCATGAGAATCGTCAGATGTTATCTTCTACAAATCAAATAACAGGGGAGAATCATCCAATAACATCTCTTAGGACTAAAGACACTGAAGAAGTCTCTAATCTGATTGAATCTAGCGCCGCTCGACCAGATCCCTTTGACATGGGCCCACTTAACGATGAAGTGACGAAAGAGCTGAATCAGCCAGACATTGCCATGGTAGAAATACCTGTGAAAGAGTCTGTCGAGAGTTCTTCCCCTTTTGCTGCATCGGGGAGTTCCAATGGCACTATGGAGTTTTTGCAGCCTGATGAAATCTCTATTCCTTCTATCTGTGCTTCCCTTGTTCCGTTGTATCATGGGAGTCAGAGGATAAAGTTGTTACACAAAGATGTAAGTTTGCAGCTTTGCTGTAGGCATATGAAATTGCGGTTTGGAATCAATGGAAAGTTTTTTGATCATGCTGGCCGGCCACGATTGAATATTGTTTGTTACGCATCACAAAATTTGTGCAAAGTTCTTGATGCATGTGATGGCATTGCACAAAAGTTGTCTATGGATTCCGGTAGCAGCTCCGAATGGAGGCCTGTTGTGATCCGAAAAGAGGGTTTCAATAACTATCCTACAGTGAGATTACACATACAGACAGCAGTATGTGGGGATATTGCAATATACGCCACAGAGATATATCAAAAAGAGCCTTCTGGCGCTGAGGAGAGGCTCGTCTTCACCAAGTTTGATGCTTCAGCACTTAGCACCCTTTTCAAACCGCAGACTTTTGTGGATGCATTCTTCTCCTTGGATCCGTATGACTTTCAGGAGAGTGCAGGCATTAGGTTGGTGGCAAAGAAATTGATTCTTCATTCCAATTGA

Protein Analysis

507

Amino Acids

56.25

Weight (kDa)

6.05

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 13 - 175 4.4e-24 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 380
AccB1I GGYRCC 2 cut(s) 58, 380
AccBSI CCGCTC 1 cut(s) 791
AccI GTMKAC 1 cut(s) 267
AccII CGCG 1 cut(s) 219
AccIII TCCGGA 1 cut(s) 71
AciI CCGC 8 cut(s) 165, 177, 217, 234, 412, 789, 1078, 1418
AclWI GGATC 6 cut(s) 27, 63, 794, 1237, 1445, 1458
AcoI YGGCCR 2 cut(s) 1114, 1118
AcsI RAATTY 2 cut(s) 95, 1153
AcuI CTGAAG 3 cut(s) 545, 777, 1377
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 1 cut(s) 382
AfiI CCNNNNNNNGG 3 cut(s) 465, 1230, 1252
AgsI TTSAA 6 cut(s) 248, 518, 779, 1129, 1261, 1414
AjiI CACGTC 1 cut(s) 46
AluBI AGCT 7 cut(s) 127, 136, 144, 428, 844, 1054, 1221
AluI AGCT 7 cut(s) 127, 136, 144, 428, 844, 1054, 1221
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 3 cut(s) 44, 654, 769
AlwI GGATC 6 cut(s) 27, 63, 794, 1237, 1445, 1458
Ama87I CYCGRG 1 cut(s) 137
Aor13HI TCCGGA 1 cut(s) 71
AoxI GGCC 6 cut(s) 359, 444, 814, 1114, 1118, 1232
ApaI GGGCCC 1 cut(s) 818
ApeKI GCWGC 4 cut(s) 914, 952, 1051, 1218
ApoI RAATTY 2 cut(s) 95, 1153
Asp718I GGTACC 1 cut(s) 380
AspA2I CCTAGG 1 cut(s) 119
AspLEI GCGC 3 cut(s) 367, 788, 1358
AspS9I GGNCC 2 cut(s) 814, 815
AsuHPI GGTGA 2 cut(s) 76, 1369
AsuII TTCGAA 2 cut(s) 27, 505
AvaI CYCGRG 1 cut(s) 137
AvrII CCTAGG 1 cut(s) 119
BaeGI GKGCMC 1 cut(s) 818
BamHI GGATCC 1 cut(s) 1450
BanI GGYRCC 2 cut(s) 58, 380
BanII GRGCYC 2 cut(s) 138, 818
BbsI GAAGAC 1 cut(s) 1366
Bbv12I GWGCWC 1 cut(s) 138
BbvCI CCTCAGC 1 cut(s) 1359
BbvI GCAGC 4 cut(s) 901, 964, 1063, 1230
BccI CCATC 5 cut(s) 49, 95, 188, 436, 1175
BceAI ACGGC 2 cut(s) 209, 223
BcgI CGANNNNNNTGC 2 cut(s) 662, 696
BclI TGATCA 1 cut(s) 1105
BcoDI GTCTC 3 cut(s) 44, 654, 769
BfaI CTAG 2 cut(s) 120, 783
BfmI CTRYAG 2 cut(s) 1060, 1272
BfoI RGCGCY 2 cut(s) 789, 1359
BisI GCNGC 6 cut(s) 234, 789, 915, 953, 1052, 1219
BlnI CCTAGG 1 cut(s) 119
BlsI GCNGC 6 cut(s) 235, 790, 916, 954, 1053, 1220
BmeT110I CYCGRG 1 cut(s) 137
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 2 cut(s) 814, 815
BmiI GGNNCC 4 cut(s) 60, 382, 816, 1452
BmsI GCATC 5 cut(s) 926, 1154, 1162, 1378, 1423
BpiI GAAGAC 1 cut(s) 1366
BplI GAGNNNNNCTC 2 cut(s) 131, 163
Bpu10I CCTNAGC 2 cut(s) 642, 1359
Bpu14I TTCGAA 2 cut(s) 27, 505
BsaBI GATNNNNATC 1 cut(s) 324
BsaHI GRCGYC 1 cut(s) 222
BsaI GGTCTC 1 cut(s) 654
BsaJI CCNNGG 4 cut(s) 119, 199, 864, 1446
BsaWI WCCGGW 2 cut(s) 71, 1211
BsaXI ACNNNNNCTCC 2 cut(s) 415, 445
Bsc4I CCNNNNNNNGG 3 cut(s) 465, 1230, 1252
Bse118I RCCGGY 1 cut(s) 1116
Bse3DI GCAATG 3 cut(s) 228, 858, 1185
Bse8I GATNNNNATC 1 cut(s) 324
BseAI TCCGGA 1 cut(s) 71
BseDI CCNNGG 4 cut(s) 119, 199, 864, 1446
BseGI GGATG 3 cut(s) 502, 724, 1438
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 3 cut(s) 465, 1230, 1252
BseMI GCAATG 3 cut(s) 228, 858, 1185
BseMII CTCAG 3 cut(s) 302, 660, 1350
BseRI GAGGAG 1 cut(s) 1376
BseSI GKGCMC 1 cut(s) 818
BseXI GCAGC 4 cut(s) 901, 964, 1063, 1230
BsgI GTGCAG 1 cut(s) 1494
Bsh1236I CGCG 1 cut(s) 219
BshFI GGCC 6 cut(s) 361, 446, 816, 1116, 1120, 1234
BshNI GGYRCC 2 cut(s) 58, 380
BsiHKAI GWGCWC 1 cut(s) 138
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 3 cut(s) 72, 1117, 1212
BslI CCNNNNNNNGG 3 cut(s) 465, 1230, 1252
BsmAI GTCTC 3 cut(s) 44, 654, 769
BsmBI CGTCTC 1 cut(s) 44
BsmI GAATGC 1 cut(s) 1436
BsnI GGCC 6 cut(s) 361, 446, 816, 1116, 1120, 1234
Bso31I GGTCTC 1 cut(s) 654
BsoBI CYCGRG 1 cut(s) 137
Bsp119I TTCGAA 2 cut(s) 27, 505
Bsp120I GGGCCC 1 cut(s) 814
Bsp1286I GDGCHC 2 cut(s) 138, 818
Bsp13I TCCGGA 1 cut(s) 71
Bsp143I GATC 6 cut(s) 19, 68, 799, 1105, 1242, 1450
Bsp19I CCATGG 1 cut(s) 864
BspACI CCGC 8 cut(s) 165, 177, 217, 234, 412, 789, 1078, 1418
BspANI GGCC 6 cut(s) 361, 446, 816, 1116, 1120, 1234
BspCNI CTCAG 3 cut(s) 303, 661, 1351
BspEI TCCGGA 1 cut(s) 71
BspFNI CGCG 1 cut(s) 219
BspLI GGNNCC 4 cut(s) 60, 382, 816, 1452
BspPI GGATC 6 cut(s) 27, 63, 794, 1237, 1445, 1458
BspT104I TTCGAA 2 cut(s) 27, 505
BspT107I GGYRCC 2 cut(s) 58, 380
BspTNI GGTCTC 1 cut(s) 654
BsrBI CCGCTC 1 cut(s) 791
BsrDI GCAATG 3 cut(s) 228, 858, 1185
BsrFI RCCGGY 1 cut(s) 1116
BssAI RCCGGY 1 cut(s) 1116
BssECI CCNNGG 4 cut(s) 119, 199, 864, 1446
BssMI GATC 6 cut(s) 19, 68, 799, 1105, 1242, 1450
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 3 cut(s) 119, 864, 1446
Bst4CI ACNGT 3 cut(s) 265, 541, 1276
BstACI GRCGYC 1 cut(s) 222
BstBI TTCGAA 2 cut(s) 27, 505
BstC8I GCNNGC 5 cut(s) 349, 363, 1114, 1118, 1477
BstDEI CTNAG 6 cut(s) 311, 642, 669, 740, 1359, 1400
BstDSI CCRYGG 2 cut(s) 199, 864
BstF5I GGATG 3 cut(s) 502, 724, 1438
BstFNI CGCG 1 cut(s) 219
BstH2I RGCGCY 2 cut(s) 789, 1359
BstHHI GCGC 3 cut(s) 367, 788, 1358
BstKTI GATC 6 cut(s) 22, 71, 802, 1108, 1245, 1453
BstMAI GTCTC 3 cut(s) 44, 654, 769
BstMBI GATC 6 cut(s) 19, 68, 799, 1105, 1242, 1450
BstMWI GCNNNNNNNGC 6 cut(s) 133, 216, 230, 452, 850, 1322
BstNSI RCATGY 1 cut(s) 1179
BstSFI CTRYAG 2 cut(s) 1060, 1272
BstSLI GKGCMC 1 cut(s) 818
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 4 cut(s) 901, 964, 1063, 1230
BstV2I GAAGAC 1 cut(s) 1366
BstX2I RGATCY 2 cut(s) 799, 1450
BstYI RGATCY 2 cut(s) 799, 1450
BsuRI GGCC 6 cut(s) 361, 446, 816, 1116, 1120, 1234
BtgI CCRYGG 2 cut(s) 199, 864
BtgZI GCGATG 1 cut(s) 238
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 3 cut(s) 502, 724, 1438
BtsIMutI CAGTG 2 cut(s) 753, 1281
Cac8I GCNNGC 5 cut(s) 349, 363, 1114, 1118, 1477
CfoI GCGC 3 cut(s) 367, 788, 1358
Cfr10I RCCGGY 1 cut(s) 1116
Cfr13I GGNCC 2 cut(s) 814, 815
CseI GACGC 1 cut(s) 230
Csp6I GTAC 1 cut(s) 381
CviAII CATG 8 cut(s) 436, 661, 674, 811, 865, 1007, 1109, 1176
CviQI GTAC 1 cut(s) 381
DdeI CTNAG 6 cut(s) 311, 642, 669, 740, 1359, 1400
DpnI GATC 6 cut(s) 21, 70, 801, 1107, 1244, 1452
DpnII GATC 6 cut(s) 19, 68, 799, 1105, 1242, 1450
EaeI YGGCCR 2 cut(s) 1114, 1118
EciI GGCGGA 1 cut(s) 166
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 3 cut(s) 119, 864, 1446
Eco147I AGGCCT 1 cut(s) 1234
Eco24I GRGCYC 2 cut(s) 138, 818
Eco31I GGTCTC 1 cut(s) 654
Eco53kI GAGCTC 1 cut(s) 136
Eco57I CTGAAG 3 cut(s) 545, 777, 1377
Eco88I CYCGRG 1 cut(s) 137
EcoICRI GAGCTC 1 cut(s) 136
EcoRI GAATTC 1 cut(s) 95
EcoT14I CCWWGG 3 cut(s) 119, 864, 1446
EcoT22I ATGCAT 2 cut(s) 1177, 1438
EcoT38I GRGCYC 2 cut(s) 138, 818
ErhI CCWWGG 3 cut(s) 119, 864, 1446
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 8 cut(s) 439, 664, 677, 814, 868, 1010, 1112, 1179
FatI CATG 8 cut(s) 435, 660, 673, 810, 864, 1006, 1108, 1175
FauI CCCGC 2 cut(s) 172, 224
FauNDI CATATG 1 cut(s) 1068
FbaI TGATCA 1 cut(s) 1105
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 6 cut(s) 234, 789, 915, 953, 1052, 1219
FokI GGATG 3 cut(s) 509, 711, 1445
FriOI GRGCYC 2 cut(s) 138, 818
FseI GGCCGGCC 1 cut(s) 1120
Fsp4HI GCNGC 6 cut(s) 234, 789, 915, 953, 1052, 1219
FspBI CTAG 2 cut(s) 120, 783
GlaI GCGC 3 cut(s) 366, 787, 1357
GluI GCNGC 6 cut(s) 234, 789, 915, 953, 1052, 1219
HaeII RGCGCY 2 cut(s) 789, 1359
HaeIII GGCC 6 cut(s) 361, 446, 816, 1116, 1120, 1234
HapII CCGG 3 cut(s) 72, 1117, 1212
HgaI GACGC 1 cut(s) 230
HhaI GCGC 3 cut(s) 367, 788, 1358
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 8 cut(s) 439, 664, 677, 814, 868, 1010, 1112, 1179
Hin6I GCGC 3 cut(s) 365, 786, 1356
HinP1I GCGC 3 cut(s) 365, 786, 1356
HincII GTYRAC 1 cut(s) 405
HindII GTYRAC 1 cut(s) 405
HpaII CCGG 3 cut(s) 72, 1117, 1212
HphI GGTGA 2 cut(s) 76, 1369
Hpy166II GTNNAC 2 cut(s) 268, 405
Hpy188III TCNNGA 9 cut(s) 72, 139, 248, 335, 466, 515, 895, 1169, 1466
Hpy8I GTNNAC 2 cut(s) 268, 405
Hpy99I CGWCG 2 cut(s) 47, 224
HpyAV CCTTC 5 cut(s) 46, 249, 414, 984, 1359
HpyCH4III ACNGT 3 cut(s) 265, 541, 1276
HpyCH4IV ACGT 2 cut(s) 45, 286
HpyF10VI GCNNNNNNNGC 6 cut(s) 133, 216, 230, 452, 850, 1322
HpyF3I CTNAG 6 cut(s) 311, 642, 669, 740, 1359, 1400
HpySE526I ACGT 2 cut(s) 45, 286
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 8 cut(s) 439, 664, 677, 814, 868, 1010, 1112, 1179
HspAI GCGC 3 cut(s) 365, 786, 1356
Kpn2I TCCGGA 1 cut(s) 71
KpnI GGTACC 1 cut(s) 384
KroI GCCGGC 1 cut(s) 1116
KroNI GCCGGC 1 cut(s) 1118
Ksp22I TGATCA 1 cut(s) 1105
Kzo9I GATC 6 cut(s) 19, 68, 799, 1105, 1242, 1450
LmnI GCTCC 3 cut(s) 12, 133, 1226
Lsp1109I GCAGC 4 cut(s) 901, 964, 1063, 1230
LweI GCATC 5 cut(s) 926, 1154, 1162, 1378, 1423
MaeI CTAG 2 cut(s) 120, 783
MaeII ACGT 2 cut(s) 45, 286
MaeIII GTNAC 7 cut(s) 299, 431, 543, 623, 832, 1029, 1139
MalI GATC 6 cut(s) 21, 70, 801, 1107, 1244, 1452
MbiI CCGCTC 1 cut(s) 791
MboI GATC 6 cut(s) 19, 68, 799, 1105, 1242, 1450
MboII GAAGA 8 cut(s) 31, 451, 687, 770, 894, 1366, 1432, 1499
MfeI CAATTG 1 cut(s) 1516
MflI RGATCY 2 cut(s) 799, 1450
MhlI GDGCHC 2 cut(s) 138, 818
MluCI AATT 6 cut(s) 95, 184, 1073, 1153, 1499, 1516
MlyI GAGTC 4 cut(s) 16, 563, 896, 1021
Mph1103I ATGCAT 2 cut(s) 1177, 1438
MroI TCCGGA 1 cut(s) 71
MroNI GCCGGC 1 cut(s) 1116
MseI TTAA 1 cut(s) 822
MslI CAYNNNNRTG 4 cut(s) 440, 659, 863, 1038
MspA1I CMGCKG 1 cut(s) 412
MspI CCGG 3 cut(s) 72, 1117, 1212
MunI CAATTG 1 cut(s) 1516
Mva1269I GAATGC 1 cut(s) 1436
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 6 cut(s) 133, 216, 230, 452, 850, 1322
NaeI GCCGGC 1 cut(s) 1118
NcoI CCATGG 1 cut(s) 864
NdeI CATATG 1 cut(s) 1068
NdeII GATC 6 cut(s) 19, 68, 799, 1105, 1242, 1450
NgoMIV GCCGGC 1 cut(s) 1116
NlaIII CATG 8 cut(s) 439, 664, 677, 814, 868, 1010, 1112, 1179
NlaIV GGNNCC 4 cut(s) 60, 382, 816, 1452
NmuCI GTSAC 3 cut(s) 299, 623, 832
NsiI ATGCAT 2 cut(s) 1177, 1438
NspI RCATGY 1 cut(s) 1179
NspV TTCGAA 2 cut(s) 27, 505
PaeR7I CTCGAG 1 cut(s) 137
PceI AGGCCT 1 cut(s) 1234
PcsI WCGNNNNNNNCGW 1 cut(s) 59
PctI GAATGC 1 cut(s) 1436
PdiI GCCGGC 1 cut(s) 1118
PflFI GACNNNGTC 1 cut(s) 224
PkrI GCNGC 6 cut(s) 235, 790, 916, 954, 1053, 1220
PleI GAGTC 4 cut(s) 15, 562, 895, 1020
PpsI GAGTC 4 cut(s) 15, 562, 895, 1020
Psp124BI GAGCTC 1 cut(s) 138
PspN4I GGNNCC 4 cut(s) 60, 382, 816, 1452
PspOMI GGGCCC 1 cut(s) 814
PspPI GGNCC 2 cut(s) 814, 815
PsuI RGATCY 2 cut(s) 799, 1450
PsyI GACNNNGTC 1 cut(s) 224
RigI GGCCGGCC 1 cut(s) 1120
RsaI GTAC 1 cut(s) 382
RsaNI GTAC 1 cut(s) 381
RseI CAYNNNNRTG 4 cut(s) 440, 659, 863, 1038
SacI GAGCTC 1 cut(s) 138
SaqAI TTAA 1 cut(s) 822
SatI GCNGC 6 cut(s) 234, 789, 915, 953, 1052, 1219
Sau3AI GATC 6 cut(s) 19, 68, 799, 1105, 1242, 1450
Sau96I GGNCC 2 cut(s) 814, 815
SchI GAGTC 4 cut(s) 16, 563, 896, 1021
SduI GDGCHC 2 cut(s) 138, 818
SfaNI GCATC 5 cut(s) 926, 1154, 1162, 1378, 1423
SfcI CTRYAG 2 cut(s) 1060, 1272
Sfr274I CTCGAG 1 cut(s) 137
SfuI TTCGAA 2 cut(s) 27, 505
SlaI CTCGAG 1 cut(s) 137
SmiMI CAYNNNNRTG 4 cut(s) 440, 659, 863, 1038
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 6 cut(s) 95, 184, 1073, 1153, 1499, 1516
SseBI AGGCCT 1 cut(s) 1234
SsiI CCGC 8 cut(s) 165, 177, 217, 234, 412, 789, 1078, 1418
SspI AATATT 1 cut(s) 1132
SspMI CTAG 2 cut(s) 120, 783
SstI GAGCTC 1 cut(s) 138
StuI AGGCCT 1 cut(s) 1234
StyI CCWWGG 3 cut(s) 119, 864, 1446
TaaI ACNGT 3 cut(s) 265, 541, 1276
TaiI ACGT 2 cut(s) 48, 289
TaqI TCGA 9 cut(s) 27, 42, 104, 138, 160, 505, 557, 793, 894
TasI AATT 6 cut(s) 95, 184, 1073, 1153, 1499, 1516
TauI GCSGC 2 cut(s) 236, 791
Tru1I TTAA 1 cut(s) 822
Tru9I TTAA 1 cut(s) 822
TscAI CASTG 2 cut(s) 760, 1281
TseFI GTSAC 3 cut(s) 299, 623, 832
TseI GCWGC 4 cut(s) 914, 952, 1051, 1218
Tsp45I GTSAC 3 cut(s) 299, 623, 832
TspDTI ATGAA 7 cut(s) 452, 524, 677, 843, 975, 1085, 1499
TspGWI ACGGA 4 cut(s) 184, 188, 987, 1443
TspRI CASTG 2 cut(s) 760, 1281
Tth111I GACNNNGTC 1 cut(s) 224
XapI RAATTY 2 cut(s) 95, 1153
XceI RCATGY 1 cut(s) 1179
XcmI CCANNNNNNNNNTGG 2 cut(s) 391, 937
XhoI CTCGAG 1 cut(s) 137
XmaJI CCTAGG 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 267
XspI CTAG 2 cut(s) 120, 783
Zsp2I ATGCAT 2 cut(s) 1177, 1438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.