RchiOBHm_Chr7g0181951

EXOIII

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004362
Physical Location & Seq
Forward (+)
3390189 .. 3392634
2446 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16229

Sequence Viewer

Length: 1221 bp
ATGAATAGATCCGCCCCTGCCGGTCATAATATATTGAGGTTTGATTGTCCCCTGATCCGGGAGGCTTTTGCAAATGTTGGTCGACCTGCACCAGAACCCAAAGGTACAATTGATTCTTTGGATTTGTTGACTCAGCGATTTGGAAGGAGAGCTGGTAACATGAAGATGGCCACTCTTGCAAATTATTTTGGTCTTGGACAACAAACACACAGGAGTTTAGATGATGTTCGAATGAATCTTGAAGTACTCAAATCTTGTGCTACAGTTTTATTCCTGGAGTACAGCCTCCCGGACATATTAACAGAGAACAGCTGGGTTTCTCCAAATGCTACAACAAGAAGTCGAAGTGTTGGAAAGTCATCTCCTGAGGGGTCAAGCATGAACATAAATATTTCAAATCAAACAGGGGACAATCATCCAATAACATCTCTTAGGACTGAGACCACACAAGAAGTCTCTAATTTGCTTGAGACTGTCGAGTCCGGCACAAATCGACCAGATCTTTCTGACATGGGCCCACTTATCAATGAAGCGAAGAATGAGTTAGATCAGCAAGACATCAACATGGTGGAAACACCTGCACAAGAGTTTCCCAACAGTTCTCTCAATGCTTCTGTGACCAACAACTGTAATGCCACCATGGATTTTTTACAGCTCAATCAAGTTTTCATTCCTTATATCAGTGCTTCGCTTGTTCCATTGTATCGTGGGAGTCGAAGGATAAAGTTATTACACAAAGATGTCACTTTGCAGCTTTGCTGCAGTCCTATGAAATTGCGGTTTGGAATCAATATGAAGTTTTTTGATCATGCTGGCCGACCACGACTGAATATTGTCTTGGATGCACCACCAAAATTATGTGAAGTTCTTGATGCATGCGATGGCCTTGCACAGAAGTTGTCTTTGGACTCTGGAAGTAGCTCTCAATGGTGGCCTGTTGTGATCCGCAATGAGAGCTTCTACAACTACCCTACTGTGAGATTGCACATAGAAACTGCAGTATACGGGGACATTGCAATCTACGCCACAGAGATATATCAAAGGGACCCCTCTGGCACCGAGCAAAGGCTAGTCTTCTCTAAATTTGATGCTTCAGAACTTGGTGCCTTGTTCACCCGAGGGATGTTTATGGATGTGTTCTTCTCTTTGGATCCATATGACTATCAGCAGAGTGCAGGCATCAGGTTGGTGGCAAAGAAACTGATTATTCATTCCAATTGA

Protein Analysis

406

Amino Acids

45.33

Weight (kDa)

5.86

Isoelectric Point (pI)

52.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 7 - 83 4.5e-09 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 586
AasI GACNNNNNNGTC 1 cut(s) 478
Acc36I ACCTGC 2 cut(s) 94, 586
AccB1I GGYRCC 2 cut(s) 1055, 1103
AccI GTMKAC 2 cut(s) 82, 1002
AciI CCGC 3 cut(s) 12, 778, 946
AclWI GGATC 5 cut(s) 3, 49, 937, 1145, 1158
AcoI YGGCCR 2 cut(s) 168, 814
AcsI RAATTY 1 cut(s) 1082
AcuI CTGAAG 1 cut(s) 1077
AfaI GTAC 3 cut(s) 106, 246, 281
AfiI CCNNNNNNNGG 3 cut(s) 57, 58, 1065
AgsI TTSAA 2 cut(s) 242, 396
AjnI CCWGG 1 cut(s) 273
AjuI GAANNNNNNNTTGG 4 cut(s) 821, 853, 887, 919
AluBI AGCT 6 cut(s) 152, 312, 655, 754, 921, 957
AluI AGCT 6 cut(s) 152, 312, 655, 754, 921, 957
Alw26I GTCTC 3 cut(s) 434, 460, 464
AlwI GGATC 5 cut(s) 3, 49, 937, 1145, 1158
Ama87I CYCGRG 1 cut(s) 1116
AoxI GGCC 5 cut(s) 168, 514, 814, 883, 932
ApaI GGGCCC 1 cut(s) 518
ApeKI GCWGC 2 cut(s) 751, 759
ApoI RAATTY 1 cut(s) 1082
AspS9I GGNCC 3 cut(s) 514, 515, 1045
AsuC2I CCSGG 2 cut(s) 59, 290
AsuHPI GGTGA 1 cut(s) 1105
AsuII TTCGAA 1 cut(s) 229
AvaI CYCGRG 1 cut(s) 1116
AvaII GGWCC 1 cut(s) 1045
AxyI CCTNAGG 1 cut(s) 366
BaeGI GKGCMC 1 cut(s) 518
BalI TGGCCA 1 cut(s) 170
BamHI GGATCC 1 cut(s) 1150
BanI GGYRCC 2 cut(s) 1055, 1103
BanII GRGCYC 1 cut(s) 518
BbsI GAAGAC 1 cut(s) 1066
BbvI GCAGC 2 cut(s) 746, 763
BccI CCATC 2 cut(s) 160, 875
BcgI CGANNNNNNTGC 2 cut(s) 869, 903
BciT130I CCWGG 1 cut(s) 275
BclI TGATCA 1 cut(s) 805
BcnI CCSGG 2 cut(s) 59, 290
BcoDI GTCTC 3 cut(s) 434, 460, 464
BfaI CTAG 1 cut(s) 1070
BfmI CTRYAG 3 cut(s) 261, 760, 996
BfuAI ACCTGC 2 cut(s) 94, 586
BglII AGATCT 1 cut(s) 499
BisI GCNGC 2 cut(s) 752, 760
BlsI GCNGC 2 cut(s) 753, 761
BmcAI AGTACT 1 cut(s) 246
Bme1390I CCNGG 3 cut(s) 59, 275, 290
Bme18I GGWCC 1 cut(s) 1045
BmeT110I CYCGRG 1 cut(s) 1116
BmgT120I GGNCC 3 cut(s) 514, 515, 1045
BmiI GGNNCC 6 cut(s) 516, 1046, 1047, 1057, 1105, 1152
BmrFI CCNGG 3 cut(s) 59, 275, 290
BmsI GCATC 4 cut(s) 832, 862, 1078, 1188
BpiI GAAGAC 1 cut(s) 1066
BpmI CTGGAG 1 cut(s) 296
Bpu14I TTCGAA 1 cut(s) 229
BpuEI CTTGAG 1 cut(s) 488
BpuMI CCSGG 2 cut(s) 59, 290
BsaI GGTCTC 1 cut(s) 434
BsaJI CCNNGG 2 cut(s) 639, 1117
BsaXI ACNNNNNCTCC 2 cut(s) 139, 169
Bsc4I CCNNNNNNNGG 3 cut(s) 57, 58, 1065
Bse118I RCCGGY 1 cut(s) 20
Bse21I CCTNAGG 1 cut(s) 366
Bse3DI GCAATG 2 cut(s) 955, 1011
BseBI CCWGG 1 cut(s) 275
BseDI CCNNGG 2 cut(s) 639, 1117
BseGI GGATG 4 cut(s) 415, 847, 1128, 1138
BseLI CCNNNNNNNGG 3 cut(s) 57, 58, 1065
BseMI GCAATG 2 cut(s) 955, 1011
BseMII CTCAG 3 cut(s) 146, 357, 429
BseSI GKGCMC 1 cut(s) 518
BseXI GCAGC 2 cut(s) 746, 763
BseYI CCCAGC 1 cut(s) 312
BsgI GTGCAG 3 cut(s) 72, 564, 1194
BshFI GGCC 5 cut(s) 170, 516, 816, 885, 934
BshNI GGYRCC 2 cut(s) 1055, 1103
BsiHKCI CYCGRG 1 cut(s) 1116
BsiSI CCGG 4 cut(s) 21, 58, 290, 483
BslFI GGGAC 4 cut(s) 33, 422, 1022, 1058
BslI CCNNNNNNNGG 3 cut(s) 57, 58, 1065
BsmAI GTCTC 3 cut(s) 434, 460, 464
BsmFI GGGAC 4 cut(s) 33, 422, 1022, 1058
BsnI GGCC 5 cut(s) 170, 516, 816, 885, 934
Bso31I GGTCTC 1 cut(s) 434
BsoBI CYCGRG 1 cut(s) 1116
Bsp119I TTCGAA 1 cut(s) 229
Bsp120I GGGCCC 1 cut(s) 514
Bsp1286I GDGCHC 1 cut(s) 518
Bsp143I GATC 7 cut(s) 8, 54, 499, 547, 805, 942, 1150
Bsp19I CCATGG 1 cut(s) 639
BspACI CCGC 3 cut(s) 12, 778, 946
BspANI GGCC 5 cut(s) 170, 516, 816, 885, 934
BspCNI CTCAG 3 cut(s) 145, 358, 430
BspLI GGNNCC 6 cut(s) 516, 1046, 1047, 1057, 1105, 1152
BspMAI CTGCAG 2 cut(s) 764, 1000
BspMI ACCTGC 2 cut(s) 94, 586
BspPI GGATC 5 cut(s) 3, 49, 937, 1145, 1158
BspT104I TTCGAA 1 cut(s) 229
BspT107I GGYRCC 2 cut(s) 1055, 1103
BspTNI GGTCTC 1 cut(s) 434
BsrDI GCAATG 2 cut(s) 955, 1011
BsrFI RCCGGY 1 cut(s) 20
BssAI RCCGGY 1 cut(s) 20
BssECI CCNNGG 2 cut(s) 639, 1117
BssMI GATC 7 cut(s) 8, 54, 499, 547, 805, 942, 1150
BssNAI GTATAC 1 cut(s) 1003
BssT1I CCWWGG 1 cut(s) 639
Bst1107I GTATAC 1 cut(s) 1003
Bst2UI CCWGG 1 cut(s) 275
Bst4CI ACNGT 5 cut(s) 265, 475, 599, 629, 976
BstBI TTCGAA 1 cut(s) 229
BstC8I GCNNGC 3 cut(s) 814, 877, 1177
BstDEI CTNAG 4 cut(s) 132, 366, 431, 438
BstDSI CCRYGG 1 cut(s) 639
BstF5I GGATG 4 cut(s) 415, 847, 1128, 1138
BstKTI GATC 7 cut(s) 11, 57, 502, 550, 808, 945, 1153
BstMAI GTCTC 3 cut(s) 434, 460, 464
BstMBI GATC 7 cut(s) 8, 54, 499, 547, 805, 942, 1150
BstMWI GCNNNNNNNGC 3 cut(s) 176, 954, 1022
BstNI CCWGG 1 cut(s) 275
BstNSI RCATGY 1 cut(s) 879
BstSCI CCNGG 3 cut(s) 57, 273, 288
BstSFI CTRYAG 3 cut(s) 261, 760, 996
BstSLI GKGCMC 1 cut(s) 518
BstV1I GCAGC 2 cut(s) 746, 763
BstV2I GAAGAC 1 cut(s) 1066
BstX2I RGATCY 3 cut(s) 8, 499, 1150
BstYI RGATCY 3 cut(s) 8, 499, 1150
BstZ17I GTATAC 1 cut(s) 1003
Bsu36I CCTNAGG 1 cut(s) 366
BsuRI GGCC 5 cut(s) 170, 516, 816, 885, 934
BtgI CCRYGG 1 cut(s) 639
BtgZI GCGATG 1 cut(s) 894
BtsCI GGATG 4 cut(s) 415, 847, 1128, 1138
BtsIMutI CAGTG 1 cut(s) 688
BveI ACCTGC 2 cut(s) 94, 586
Cac8I GCNNGC 3 cut(s) 814, 877, 1177
Cfr10I RCCGGY 1 cut(s) 20
Cfr13I GGNCC 3 cut(s) 514, 515, 1045
Csp6I GTAC 3 cut(s) 105, 245, 280
CviAII CATG 7 cut(s) 160, 379, 511, 565, 640, 809, 876
CviQI GTAC 3 cut(s) 105, 245, 280
DdeI CTNAG 4 cut(s) 132, 366, 431, 438
DpnI GATC 7 cut(s) 10, 56, 501, 549, 807, 944, 1152
DpnII GATC 7 cut(s) 8, 54, 499, 547, 805, 942, 1150
DrdI GACNNNNNNGTC 1 cut(s) 478
DseDI GACNNNNNNGTC 1 cut(s) 478
EaeI YGGCCR 2 cut(s) 168, 814
Eco130I CCWWGG 1 cut(s) 639
Eco24I GRGCYC 1 cut(s) 518
Eco31I GGTCTC 1 cut(s) 434
Eco47I GGWCC 1 cut(s) 1045
Eco57I CTGAAG 1 cut(s) 1077
Eco81I CCTNAGG 1 cut(s) 366
Eco88I CYCGRG 1 cut(s) 1116
EcoO109I RGGNCCY 1 cut(s) 1045
EcoRII CCWGG 1 cut(s) 273
EcoT14I CCWWGG 1 cut(s) 639
EcoT22I ATGCAT 1 cut(s) 877
EcoT38I GRGCYC 1 cut(s) 518
ErhI CCWWGG 1 cut(s) 639
FaeI CATG 7 cut(s) 163, 382, 514, 568, 643, 812, 879
FaqI GGGAC 4 cut(s) 33, 422, 1022, 1058
FatI CATG 7 cut(s) 159, 378, 510, 564, 639, 808, 875
FauNDI CATATG 1 cut(s) 1156
FbaI TGATCA 1 cut(s) 805
FblI GTMKAC 2 cut(s) 82, 1002
Fnu4HI GCNGC 2 cut(s) 752, 760
FokI GGATG 4 cut(s) 402, 854, 1135, 1145
FriOI GRGCYC 1 cut(s) 518
Fsp4HI GCNGC 2 cut(s) 752, 760
FspBI CTAG 1 cut(s) 1070
GluI GCNGC 2 cut(s) 752, 760
GsaI CCCAGC 1 cut(s) 316
GsuI CTGGAG 1 cut(s) 296
HaeIII GGCC 5 cut(s) 170, 516, 816, 885, 934
HapII CCGG 4 cut(s) 21, 58, 290, 483
Hin1II CATG 7 cut(s) 163, 382, 514, 568, 643, 812, 879
HincII GTYRAC 2 cut(s) 83, 129
HindII GTYRAC 2 cut(s) 83, 129
HinfI GANTC 7 cut(s) 113, 130, 235, 479, 712, 786, 908
HpaII CCGG 4 cut(s) 21, 58, 290, 483
HphI GGTGA 1 cut(s) 1105
Hpy166II GTNNAC 4 cut(s) 83, 129, 1003, 1113
Hpy188I TCNGA 2 cut(s) 508, 1096
Hpy188III TCNNGA 4 cut(s) 239, 365, 869, 912
Hpy8I GTNNAC 4 cut(s) 83, 129, 1003, 1113
HpyAV CCTTC 2 cut(s) 138, 711
HpyCH4III ACNGT 5 cut(s) 265, 475, 599, 629, 976
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 954, 1022
HpyF3I CTNAG 4 cut(s) 132, 366, 431, 438
Hsp92II CATG 7 cut(s) 163, 382, 514, 568, 643, 812, 879
KflI GGGWCCC 1 cut(s) 1045
Ksp22I TGATCA 1 cut(s) 805
Kzo9I GATC 7 cut(s) 8, 54, 499, 547, 805, 942, 1150
Lsp1109I GCAGC 2 cut(s) 746, 763
LweI GCATC 4 cut(s) 832, 862, 1078, 1188
MaeI CTAG 1 cut(s) 1070
MaeIII GTNAC 3 cut(s) 155, 616, 742
MalI GATC 7 cut(s) 10, 56, 501, 549, 807, 944, 1152
MboI GATC 7 cut(s) 8, 54, 499, 547, 805, 942, 1150
MboII GAAGA 4 cut(s) 175, 547, 1066, 1132
MfeI CAATTG 2 cut(s) 108, 1216
MflI RGATCY 3 cut(s) 8, 499, 1150
MhlI GDGCHC 1 cut(s) 518
MlsI TGGCCA 1 cut(s) 170
MluCI AATT 7 cut(s) 108, 181, 460, 773, 854, 1082, 1216
MluNI TGGCCA 1 cut(s) 170
MlyI GAGTC 4 cut(s) 124, 488, 721, 902
MmeI TCCRAC 1 cut(s) 331
MnlI CCTC 6 cut(s) 30, 55, 296, 361, 1060, 1112
Mox20I TGGCCA 1 cut(s) 170
Mph1103I ATGCAT 1 cut(s) 877
MscI TGGCCA 1 cut(s) 170
MseI TTAA 1 cut(s) 299
MslI CAYNNNNRTG 3 cut(s) 164, 563, 738
Msp20I TGGCCA 1 cut(s) 170
MspA1I CMGCKG 1 cut(s) 312
MspI CCGG 4 cut(s) 21, 58, 290, 483
MspR9I CCNGG 3 cut(s) 59, 275, 290
MunI CAATTG 2 cut(s) 108, 1216
MvaI CCWGG 1 cut(s) 275
MwoI GCNNNNNNNGC 3 cut(s) 176, 954, 1022
NciI CCSGG 2 cut(s) 59, 290
NcoI CCATGG 1 cut(s) 639
NdeI CATATG 1 cut(s) 1156
NdeII GATC 7 cut(s) 8, 54, 499, 547, 805, 942, 1150
NlaIII CATG 7 cut(s) 163, 382, 514, 568, 643, 812, 879
NlaIV GGNNCC 6 cut(s) 516, 1046, 1047, 1057, 1105, 1152
NmuCI GTSAC 2 cut(s) 616, 742
NsiI ATGCAT 1 cut(s) 877
NspI RCATGY 1 cut(s) 879
NspV TTCGAA 1 cut(s) 229
PaeI GCATGC 1 cut(s) 879
PaqCI CACCTGC 1 cut(s) 586
PcsI WCGNNNNNNNCGW 1 cut(s) 712
PfeI GAWTC 3 cut(s) 113, 235, 786
PfoI TCCNGGA 3 cut(s) 57, 273, 288
PkrI GCNGC 2 cut(s) 753, 761
PleI GAGTC 4 cut(s) 124, 487, 720, 902
PpsI GAGTC 4 cut(s) 124, 487, 720, 902
PpuMI RGGWCCY 1 cut(s) 1045
Psp5II RGGWCCY 1 cut(s) 1045
Psp6I CCWGG 1 cut(s) 273
PspFI CCCAGC 1 cut(s) 312
PspGI CCWGG 1 cut(s) 273
PspN4I GGNNCC 6 cut(s) 516, 1046, 1047, 1057, 1105, 1152
PspOMI GGGCCC 1 cut(s) 514
PspPI GGNCC 3 cut(s) 514, 515, 1045
PspPPI RGGWCCY 1 cut(s) 1045
PstI CTGCAG 2 cut(s) 764, 1000
PsuI RGATCY 3 cut(s) 8, 499, 1150
PvuII CAGCTG 1 cut(s) 312
RsaI GTAC 3 cut(s) 106, 246, 281
RsaNI GTAC 3 cut(s) 105, 245, 280
RseI CAYNNNNRTG 3 cut(s) 164, 563, 738
SalI GTCGAC 1 cut(s) 81
SaqAI TTAA 1 cut(s) 299
SatI GCNGC 2 cut(s) 752, 760
Sau3AI GATC 7 cut(s) 8, 54, 499, 547, 805, 942, 1150
Sau96I GGNCC 3 cut(s) 514, 515, 1045
ScaI AGTACT 1 cut(s) 246
SchI GAGTC 4 cut(s) 124, 488, 721, 902
ScrFI CCNGG 3 cut(s) 59, 275, 290
SduI GDGCHC 1 cut(s) 518
SfaNI GCATC 4 cut(s) 832, 862, 1078, 1188
SfcI CTRYAG 3 cut(s) 261, 760, 996
SfuI TTCGAA 1 cut(s) 229
SinI GGWCC 1 cut(s) 1045
SmiMI CAYNNNNRTG 3 cut(s) 164, 563, 738
SmlI CTYRAG 1 cut(s) 467
SmoI CTYRAG 1 cut(s) 467
SphI GCATGC 1 cut(s) 879
Sse9I AATT 7 cut(s) 108, 181, 460, 773, 854, 1082, 1216
SsiI CCGC 3 cut(s) 12, 778, 946
SspI AATATT 2 cut(s) 391, 832
SspMI CTAG 1 cut(s) 1070
StyD4I CCNGG 3 cut(s) 57, 273, 288
StyI CCWWGG 1 cut(s) 639
TaaI ACNGT 5 cut(s) 265, 475, 599, 629, 976
TaqI TCGA 6 cut(s) 82, 229, 343, 477, 493, 715
TasI AATT 7 cut(s) 108, 181, 460, 773, 854, 1082, 1216
TatI WGTACW 2 cut(s) 244, 279
TfiI GAWTC 3 cut(s) 113, 235, 786
Tru1I TTAA 1 cut(s) 299
Tru9I TTAA 1 cut(s) 299
TscAI CASTG 1 cut(s) 688
TseFI GTSAC 2 cut(s) 616, 742
TseI GCWGC 2 cut(s) 751, 759
Tsp45I GTSAC 2 cut(s) 616, 742
TspDTI ATGAA 9 cut(s) 17, 176, 248, 395, 543, 658, 785, 809, 1199
TspRI CASTG 1 cut(s) 688
VpaK11BI GGWCC 1 cut(s) 1045
XapI RAATTY 1 cut(s) 1082
XceI RCATGY 1 cut(s) 879
XmiI GTMKAC 2 cut(s) 82, 1002
XspI CTAG 1 cut(s) 1070
ZrmI AGTACT 1 cut(s) 246
Zsp2I ATGCAT 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.