AT2G04520

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003208
Physical Location & Seq
Reverse (-)
1574562 .. 1575960
1399 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G04520.1

Sequence Viewer

Length: 438 bp
ATGCCGAAGAACAAGGGAAAGGGAGGAAAGAACAGGAAGAGAGGAAAGAACGAAGCAGATGACGAGAAGAGAGAGCTTATATTCAAAGAAGATGGACAAGAATACGCACAAGTCCTTCGTATGCTTGGAAATGGAAGATGTGAAGCTATGTGTATCGATGGAACCAAGCGTCTTTGCCATATCCGTGGTAAGATGCATAAGAAGGTTTGGATTGCAGCAGGTGATATTGTGCTTGTTGGTTTGAGGGATTACCAAGATGACAAAGCGGATGTGATCCTTAAGTACATGTCTGATGAGGCTAGGCTTCTTAAGGCTTATGGTGAGCTTCCAGAGAATACTCGTCTTAATGAAGGAATTGTTGGTGATCTTGAGGATGATGATGATAATAATGATGATGACTATGTTGAGTTTGAGGACGAAGATATCGATAGGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

145

Amino Acids

16.64

Weight (kDa)

4.83

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-1a PF01176 32 - 93 6.2e-25 Translation initiation factor 1A / IF-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 209
Acc36I ACCTGC 1 cut(s) 209
AciI CCGC 1 cut(s) 266
AclWI GGATC 1 cut(s) 268
AfaI GTAC 1 cut(s) 284
AflII CTTAAG 2 cut(s) 278, 308
AflIII ACRYGT 1 cut(s) 285
AgsI TTSAA 1 cut(s) 85
AjuI GAANNNNNNNTTGG 2 cut(s) 342, 374
AluBI AGCT 3 cut(s) 76, 146, 325
AluI AGCT 3 cut(s) 76, 146, 325
AlwI GGATC 1 cut(s) 268
ApeKI GCWGC 1 cut(s) 215
AsuHPI GGTGA 3 cut(s) 233, 332, 374
BbvI GCAGC 1 cut(s) 227
BccI CCATC 2 cut(s) 86, 152
BfaI CTAG 1 cut(s) 300
BfrI CTTAAG 2 cut(s) 278, 308
BfuAI ACCTGC 1 cut(s) 209
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
BmiI GGNNCC 1 cut(s) 163
BmsI GCATC 1 cut(s) 183
BpuEI CTTGAG 1 cut(s) 389
Bsa29I ATCGAT 2 cut(s) 156, 426
BsaJI CCNNGG 1 cut(s) 184
BseCI ATCGAT 2 cut(s) 156, 426
BseDI CCNNGG 1 cut(s) 184
BseGI GGATG 2 cut(s) 274, 379
BseXI GCAGC 1 cut(s) 227
BshVI ATCGAT 2 cut(s) 156, 426
Bsp143I GATC 3 cut(s) 273, 364, 432
BspACI CCGC 1 cut(s) 266
BspDI ATCGAT 2 cut(s) 156, 426
BspLI GGNNCC 1 cut(s) 163
BspMI ACCTGC 1 cut(s) 209
BspPI GGATC 1 cut(s) 268
BspTI CTTAAG 2 cut(s) 278, 308
BssECI CCNNGG 1 cut(s) 184
BssMI GATC 3 cut(s) 273, 364, 432
Bst6I CTCTTC 2 cut(s) 32, 62
BstAFI CTTAAG 2 cut(s) 278, 308
BstDSI CCRYGG 1 cut(s) 184
BstF5I GGATG 2 cut(s) 274, 379
BstKTI GATC 3 cut(s) 276, 367, 435
BstMBI GATC 3 cut(s) 273, 364, 432
BstNSI RCATGY 1 cut(s) 289
BstV1I GCAGC 1 cut(s) 227
BstX2I RGATCY 1 cut(s) 432
BstXI CCANNNNNNTGG 1 cut(s) 185
BstYI RGATCY 1 cut(s) 432
Bsu15I ATCGAT 2 cut(s) 156, 426
BsuTUI ATCGAT 2 cut(s) 156, 426
BtgI CCRYGG 1 cut(s) 184
BtsCI GGATG 2 cut(s) 274, 379
BveI ACCTGC 1 cut(s) 209
ClaI ATCGAT 2 cut(s) 156, 426
CseI GACGC 1 cut(s) 158
Csp6I GTAC 1 cut(s) 283
CviAII CATG 1 cut(s) 286
CviJI RGCY 6 cut(s) 76, 146, 299, 304, 314, 325
CviKI_1 RGCY 6 cut(s) 76, 146, 299, 304, 314, 325
CviQI GTAC 1 cut(s) 283
DpnI GATC 3 cut(s) 275, 366, 434
DpnII GATC 3 cut(s) 273, 364, 432
Eam1104I CTCTTC 2 cut(s) 32, 62
EarI CTCTTC 2 cut(s) 32, 62
Eco32I GATATC 1 cut(s) 424
EcoRV GATATC 1 cut(s) 424
EcoT22I ATGCAT 1 cut(s) 198
FaeI CATG 1 cut(s) 289
FaiI YATR 8 cut(s) 80, 122, 149, 180, 198, 287, 318, 402
FatI CATG 1 cut(s) 285
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 2 cut(s) 281, 386
Fsp4HI GCNGC 1 cut(s) 216
FspBI CTAG 1 cut(s) 300
GluI GCNGC 1 cut(s) 216
HgaI GACGC 1 cut(s) 158
Hin1II CATG 1 cut(s) 289
HphI GGTGA 3 cut(s) 233, 332, 374
Hpy188I TCNGA 1 cut(s) 292
Hpy188III TCNNGA 2 cut(s) 329, 368
HpyAV CCTTC 3 cut(s) 125, 196, 344
HpyCH4V TGCA 2 cut(s) 196, 215
Hsp92II CATG 1 cut(s) 289
Kzo9I GATC 3 cut(s) 273, 364, 432
LpnPI CCDG 3 cut(s) 19, 204, 342
Lsp1109I GCAGC 1 cut(s) 227
LweI GCATC 1 cut(s) 183
MaeI CTAG 1 cut(s) 300
MalI GATC 3 cut(s) 275, 366, 434
MboI GATC 3 cut(s) 273, 364, 432
MboII GAAGA 6 cut(s) 19, 49, 79, 101, 147, 431
MflI RGATCY 1 cut(s) 432
MluCI AATT 1 cut(s) 354
MnlI CCTC 6 cut(s) 17, 35, 237, 289, 364, 406
Mph1103I ATGCAT 1 cut(s) 198
MseI TTAA 3 cut(s) 279, 309, 345
MslI CAYNNNNRTG 1 cut(s) 183
MspCI CTTAAG 2 cut(s) 278, 308
NdeII GATC 3 cut(s) 273, 364, 432
NlaIII CATG 1 cut(s) 289
NlaIV GGNNCC 1 cut(s) 163
NsiI ATGCAT 1 cut(s) 198
NspI RCATGY 1 cut(s) 289
PaqCI CACCTGC 1 cut(s) 209
PciI ACATGT 1 cut(s) 285
PcsI WCGNNNNNNNCGW 1 cut(s) 423
PkrI GCNGC 1 cut(s) 217
PscI ACATGT 1 cut(s) 285
PspN4I GGNNCC 1 cut(s) 163
PsuI RGATCY 1 cut(s) 432
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
RseI CAYNNNNRTG 1 cut(s) 183
SaqAI TTAA 3 cut(s) 279, 309, 345
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 3 cut(s) 273, 364, 432
SetI ASST 5 cut(s) 78, 148, 207, 223, 327
SfaNI GCATC 1 cut(s) 183
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 3 cut(s) 278, 308, 368
SmoI CTYRAG 3 cut(s) 278, 308, 368
Sse9I AATT 1 cut(s) 354
SsiI CCGC 1 cut(s) 266
SspMI CTAG 1 cut(s) 300
TaqI TCGA 2 cut(s) 156, 426
TasI AATT 1 cut(s) 354
TatI WGTACW 1 cut(s) 282
Tru1I TTAA 3 cut(s) 279, 309, 345
Tru9I TTAA 3 cut(s) 279, 309, 345
TseI GCWGC 1 cut(s) 215
TspDTI ATGAA 1 cut(s) 363
TspGWI ACGGA 1 cut(s) 173
Vha464I CTTAAG 2 cut(s) 278, 308
XceI RCATGY 1 cut(s) 289
XspI CTAG 1 cut(s) 300
Zsp2I ATGCAT 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.