Rorug02G0461600

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003208
Physical Location & Seq
Reverse (-)
59327344 .. 59331025
3682 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0461600.1

Sequence Viewer

Length: 843 bp
ATGGACCTGGCTTTGGCGTCTCCGTTGTTCCCTTCAAATGTAACAAGAGGTGTCGATAAGATTAAGCCTTTTAGCTCAATGTTTGCCACCCGAAAGGTGCCTGCTCGAAAATTGTTTCCTTGTTTGGCAGCAGTTGCTACCCAAACACCCCAGACAGTGGAGTTTGATGTAGAGAGCAAGAAATATGAGCTGTTGAGAGCTGTCCAAGACACAAAAAGGGGCCTTGTTACAACCGACGATCAACGCTCTTCTATAGAGGAGGCTCTGGTGACTGTGGAGGGATATAACAAGGGTGCACCTTTAGACTTGGTGAAGTTGGATGGGACATGGCGGTTGCAATATACTTCTGCTCCTGATGTTCTCATTCTTCTGGAAGCAGCTGAAAGGGTATCTTTCTTTCAGGTGGGGCAGATATTTCAAAAATTTGAATGCAAAGACCAATCCAATGGCGGTATAATCCGTAATGTTGTCAGATGGAGTGTTCCACCGTTGTTAGAGGAAGAAGACGGTGCTACTCTTCTAGTTTCTGCCAAGTTTTCTGTTGTTTCCGTGCGTAACATCTATCTTCAGTTTGAAGAGATTAGTGTTCAAAATATAAAAATTGGTGAACAGCTGCAGGCTCTTATAGCTCCAGCATTACTGCCGAGGTCATTTTTGAGTTTACAGATCTTACAGTTCCTCCGCACTTTCAAAGCCCAAATTCCTGTTAGTGATCCAGGAACAGGAAGGCAATCCGTAGGAGGACTCTATTACCTTTCTTACTTGGATGCTAATATGCTTTTGGGGCGTGCGGTTGGTGGCGGAGGAGTTTTTGTTTTCACTAAAGCTCAACCCCTGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

280

Amino Acids

30.89

Weight (kDa)

7.67

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 60 - 273 5.4e-23 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 97
AccB7I CCANNNNNTGG 1 cut(s) 157
AciI CCGC 5 cut(s) 331, 450, 682, 791, 801
AclWI GGATC 1 cut(s) 707
AcsI RAATTY 2 cut(s) 422, 699
AcuI CTGAAG 1 cut(s) 551
AcyI GRCGYC 1 cut(s) 17
AfiI CCNNNNNNNGG 3 cut(s) 13, 157, 722
AgsI TTSAA 6 cut(s) 36, 419, 428, 575, 590, 691
AjnI CCWGG 3 cut(s) 6, 715, 834
AluBI AGCT 7 cut(s) 75, 190, 200, 380, 613, 629, 827
AluI AGCT 7 cut(s) 75, 190, 200, 380, 613, 629, 827
Alw21I GWGCWC 1 cut(s) 298
Alw26I GTCTC 1 cut(s) 24
Alw44I GTGCAC 1 cut(s) 294
AlwI GGATC 1 cut(s) 707
AoxI GGCC 1 cut(s) 220
ApaLI GTGCAC 1 cut(s) 294
ApeKI GCWGC 3 cut(s) 128, 377, 613
ApoI RAATTY 2 cut(s) 422, 699
AspS9I GGNCC 2 cut(s) 4, 220
AsuHPI GGTGA 3 cut(s) 280, 322, 617
AvaII GGWCC 1 cut(s) 4
BaeGI GKGCMC 1 cut(s) 298
BanI GGYRCC 1 cut(s) 97
BbsI GAAGAC 1 cut(s) 510
Bbv12I GWGCWC 1 cut(s) 298
BbvI GCAGC 3 cut(s) 140, 389, 600
BccI CCATC 2 cut(s) 314, 468
BciT130I CCWGG 3 cut(s) 8, 717, 836
BcoDI GTCTC 1 cut(s) 24
BfaI CTAG 1 cut(s) 521
BfmI CTRYAG 2 cut(s) 252, 614
BglII AGATCT 1 cut(s) 666
BisI GCNGC 3 cut(s) 129, 378, 614
BlsI GCNGC 3 cut(s) 130, 379, 615
Bme1390I CCNGG 3 cut(s) 8, 717, 836
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 2 cut(s) 4, 220
BmiI GGNNCC 2 cut(s) 99, 221
BmrFI CCNGG 3 cut(s) 8, 717, 836
BmsI GCATC 1 cut(s) 757
BpiI GAAGAC 1 cut(s) 510
BpmI CTGGAG 1 cut(s) 615
BsaHI GRCGYC 1 cut(s) 17
BsaJI CCNNGG 2 cut(s) 644, 834
BsaXI ACNNNNNCTCC 6 cut(s) 334, 364, 663, 693, 798, 828
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 157, 722
BseBI CCWGG 3 cut(s) 8, 717, 836
BseDI CCNNGG 2 cut(s) 644, 834
BseGI GGATG 2 cut(s) 325, 772
BseLI CCNNNNNNNGG 3 cut(s) 13, 157, 722
BseRI GAGGAG 2 cut(s) 272, 819
BseSI GKGCMC 1 cut(s) 298
BseXI GCAGC 3 cut(s) 140, 389, 600
BshFI GGCC 1 cut(s) 222
BshNI GGYRCC 1 cut(s) 97
BsiHKAI GWGCWC 1 cut(s) 298
BslFI GGGAC 1 cut(s) 337
BslI CCNNNNNNNGG 3 cut(s) 13, 157, 722
BsmAI GTCTC 1 cut(s) 24
BsmBI CGTCTC 1 cut(s) 24
BsmFI GGGAC 1 cut(s) 337
BsmI GAATGC 1 cut(s) 434
BsnI GGCC 1 cut(s) 222
Bsp1286I GDGCHC 1 cut(s) 298
Bsp143I GATC 3 cut(s) 238, 666, 712
BspACI CCGC 5 cut(s) 331, 450, 682, 791, 801
BspANI GGCC 1 cut(s) 222
BspLI GGNNCC 2 cut(s) 99, 221
BspMAI CTGCAG 1 cut(s) 618
BspPI GGATC 1 cut(s) 707
BspQI GCTCTTC 1 cut(s) 253
BspT107I GGYRCC 1 cut(s) 97
BssECI CCNNGG 2 cut(s) 644, 834
BssMI GATC 3 cut(s) 238, 666, 712
BssNI GRCGYC 1 cut(s) 17
Bst2UI CCWGG 3 cut(s) 8, 717, 836
Bst4CI ACNGT 5 cut(s) 157, 274, 489, 509, 675
Bst6I CTCTTC 3 cut(s) 253, 522, 570
BstACI GRCGYC 1 cut(s) 17
BstAPI GCANNNNNTGC 1 cut(s) 134
BstC8I GCNNGC 3 cut(s) 102, 618, 789
BstF5I GGATG 2 cut(s) 325, 772
BstKTI GATC 3 cut(s) 241, 669, 715
BstMAI GTCTC 1 cut(s) 24
BstMBI GATC 3 cut(s) 238, 666, 712
BstMWI GCNNNNNNNGC 3 cut(s) 134, 626, 784
BstNI CCWGG 3 cut(s) 8, 717, 836
BstSCI CCNGG 3 cut(s) 6, 715, 834
BstSFI CTRYAG 2 cut(s) 252, 614
BstSLI GKGCMC 1 cut(s) 298
BstV1I GCAGC 3 cut(s) 140, 389, 600
BstV2I GAAGAC 1 cut(s) 510
BstX2I RGATCY 1 cut(s) 666
BstXI CCANNNNNNTGG 1 cut(s) 446
BstYI RGATCY 1 cut(s) 666
BsuRI GGCC 1 cut(s) 222
BtsCI GGATG 2 cut(s) 325, 772
BtsIMutI CAGTG 1 cut(s) 162
Cac8I GCNNGC 3 cut(s) 102, 618, 789
Cfr13I GGNCC 2 cut(s) 4, 220
CseI GACGC 1 cut(s) 6
CviAII CATG 1 cut(s) 327
DpnI GATC 3 cut(s) 240, 668, 714
DpnII GATC 3 cut(s) 238, 666, 712
Eam1104I CTCTTC 3 cut(s) 253, 522, 570
EarI CTCTTC 3 cut(s) 253, 522, 570
EciI GGCGGA 1 cut(s) 816
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 1 cut(s) 551
EcoO109I RGGNCCY 1 cut(s) 220
EcoRII CCWGG 3 cut(s) 6, 715, 834
Esp3I CGTCTC 1 cut(s) 24
FaeI CATG 1 cut(s) 330
FaiI YATR 9 cut(s) 186, 254, 285, 328, 342, 455, 596, 626, 776
FalI AAGNNNNNCTT 2 cut(s) 376, 408
FaqI GGGAC 1 cut(s) 337
FatI CATG 1 cut(s) 326
Fnu4HI GCNGC 3 cut(s) 129, 378, 614
FokI GGATG 2 cut(s) 332, 779
Fsp4HI GCNGC 3 cut(s) 129, 378, 614
FspBI CTAG 1 cut(s) 521
GluI GCNGC 3 cut(s) 129, 378, 614
GsuI CTGGAG 1 cut(s) 615
HaeIII GGCC 1 cut(s) 222
HgaI GACGC 1 cut(s) 6
Hin1I GRCGYC 1 cut(s) 17
Hin1II CATG 1 cut(s) 330
HinfI GANTC 1 cut(s) 744
HphI GGTGA 3 cut(s) 280, 322, 617
Hpy166II GTNNAC 3 cut(s) 296, 608, 662
Hpy188I TCNGA 1 cut(s) 473
Hpy188III TCNNGA 2 cut(s) 353, 371
Hpy8I GTNNAC 3 cut(s) 296, 608, 662
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 2 cut(s) 42, 720
HpyCH4III ACNGT 5 cut(s) 157, 274, 489, 509, 675
HpyCH4V TGCA 4 cut(s) 296, 337, 432, 616
HpyF10VI GCNNNNNNNGC 3 cut(s) 134, 626, 784
Hsp92I GRCGYC 1 cut(s) 17
Hsp92II CATG 1 cut(s) 330
Kzo9I GATC 3 cut(s) 238, 666, 712
LguI GCTCTTC 1 cut(s) 253
LmnI GCTCC 2 cut(s) 355, 634
Lsp1109I GCAGC 3 cut(s) 140, 389, 600
LweI GCATC 1 cut(s) 757
MaeI CTAG 1 cut(s) 521
MaeIII GTNAC 4 cut(s) 40, 226, 268, 554
MalI GATC 3 cut(s) 240, 668, 714
MboI GATC 3 cut(s) 238, 666, 712
MboII GAAGA 7 cut(s) 240, 359, 509, 512, 515, 557, 587
MflI RGATCY 1 cut(s) 666
MhlI GDGCHC 1 cut(s) 298
MluCI AATT 4 cut(s) 110, 422, 600, 699
MlyI GAGTC 1 cut(s) 738
MmeI TCCRAC 1 cut(s) 297
MnlI CCTC 9 cut(s) 41, 250, 253, 271, 490, 639, 689, 734, 797
MseI TTAA 1 cut(s) 63
MspA1I CMGCKG 2 cut(s) 380, 613
MspR9I CCNGG 3 cut(s) 8, 717, 836
Mva1269I GAATGC 1 cut(s) 434
MvaI CCWGG 3 cut(s) 8, 717, 836
MwoI GCNNNNNNNGC 3 cut(s) 134, 626, 784
NdeII GATC 3 cut(s) 238, 666, 712
NlaIII CATG 1 cut(s) 330
NlaIV GGNNCC 2 cut(s) 99, 221
NmeAIII GCCGAG 1 cut(s) 669
NmuCI GTSAC 1 cut(s) 268
PciSI GCTCTTC 1 cut(s) 253
PctI GAATGC 1 cut(s) 434
PflMI CCANNNNNTGG 1 cut(s) 157
PfoI TCCNGGA 1 cut(s) 715
PkrI GCNGC 3 cut(s) 130, 379, 615
PleI GAGTC 1 cut(s) 738
PpsI GAGTC 1 cut(s) 738
Psp6I CCWGG 3 cut(s) 6, 715, 834
PspGI CCWGG 3 cut(s) 6, 715, 834
PspN4I GGNNCC 2 cut(s) 99, 221
PspPI GGNCC 2 cut(s) 4, 220
PstI CTGCAG 1 cut(s) 618
PsuI RGATCY 1 cut(s) 666
PvuII CAGCTG 2 cut(s) 380, 613
SapI GCTCTTC 1 cut(s) 253
SaqAI TTAA 1 cut(s) 63
SatI GCNGC 3 cut(s) 129, 378, 614
Sau3AI GATC 3 cut(s) 238, 666, 712
Sau96I GGNCC 2 cut(s) 4, 220
SchI GAGTC 1 cut(s) 738
ScrFI CCNGG 3 cut(s) 8, 717, 836
SduI GDGCHC 1 cut(s) 298
SfaNI GCATC 1 cut(s) 757
SfcI CTRYAG 2 cut(s) 252, 614
SinI GGWCC 1 cut(s) 4
Sse9I AATT 4 cut(s) 110, 422, 600, 699
SsiI CCGC 5 cut(s) 331, 450, 682, 791, 801
SspMI CTAG 1 cut(s) 521
StyD4I CCNGG 3 cut(s) 6, 715, 834
TaaI ACNGT 5 cut(s) 157, 274, 489, 509, 675
TaqI TCGA 2 cut(s) 54, 106
TasI AATT 4 cut(s) 110, 422, 600, 699
Tru1I TTAA 1 cut(s) 63
Tru9I TTAA 1 cut(s) 63
TscAI CASTG 1 cut(s) 162
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 3 cut(s) 128, 377, 613
Tsp45I GTSAC 1 cut(s) 268
TspGWI ACGGA 4 cut(s) 12, 449, 538, 724
TspRI CASTG 1 cut(s) 162
Van91I CCANNNNNTGG 1 cut(s) 157
VneI GTGCAC 1 cut(s) 294
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 2 cut(s) 422, 699
XspI CTAG 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.