Rorug02G0329800

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003208
Physical Location & Seq
Forward (+)
40597060 .. 40598426
1367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0329800.1

Sequence Viewer

Length: 345 bp
ATGCCAAGTCATGCCAACCTTTTCAATGATGCCATGGCTAGTGATAATCGTTTGATGACCAGCGTGTTACTTAAAGAGTGCAAGGGGGTATTCGAGGGATTGAAATCATTAGTTGATGTTGGGGGTGGTACAGGAACAATGGCCAAGGCCATTGTTGATGCATTCCCAGAAATTGACTGCACCGTACTTGATCTACCCCATGTGGTGGCTGATCTGCAAGGAAGTAAGAACTTGAAATATGCTGGAGGGGACATGTTTGAGGCAGTTCCTCCGGCAGTTGCAATTATGTTGAAGGTAAATCTCATCAATACTCTGTTGTTAGTCATGTTCTCATATTGTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

114

Amino Acids

12.17

Weight (kDa)

4.88

Isoelectric Point (pI)

34.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 4 - 99 1.4e-23 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 205
AcoI YGGCCR 1 cut(s) 141
AfaI GTAC 2 cut(s) 130, 186
AfiI CCNNNNNNNGG 1 cut(s) 205
AflIII ACRYGT 1 cut(s) 252
AgsI TTSAA 4 cut(s) 25, 103, 235, 292
AoxI GGCC 2 cut(s) 141, 147
BalI TGGCCA 1 cut(s) 143
BfaI CTAG 1 cut(s) 39
BmsI GCATC 2 cut(s) 19, 148
BpmI CTGGAG 1 cut(s) 264
BsaBI GATNNNNATC 1 cut(s) 103
BsaJI CCNNGG 2 cut(s) 33, 144
Bsc4I CCNNNNNNNGG 1 cut(s) 205
Bse8I GATNNNNATC 1 cut(s) 103
BseDI CCNNGG 2 cut(s) 33, 144
BseJI GATNNNNATC 1 cut(s) 103
BseLI CCNNNNNNNGG 1 cut(s) 205
BsgI GTGCAG 1 cut(s) 163
BshFI GGCC 2 cut(s) 143, 149
BsiSI CCGG 1 cut(s) 272
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 1 cut(s) 205
BsmFI GGGAC 1 cut(s) 263
BsmI GAATGC 1 cut(s) 161
BsnI GGCC 2 cut(s) 143, 149
Bsp143I GATC 2 cut(s) 190, 211
Bsp19I CCATGG 1 cut(s) 33
BspANI GGCC 2 cut(s) 143, 149
BssECI CCNNGG 2 cut(s) 33, 144
BssMI GATC 2 cut(s) 190, 211
BssT1I CCWWGG 2 cut(s) 33, 144
Bst4CI ACNGT 1 cut(s) 184
BstDSI CCRYGG 1 cut(s) 33
BstKTI GATC 2 cut(s) 193, 214
BstMBI GATC 2 cut(s) 190, 211
BstNSI RCATGY 1 cut(s) 256
BsuRI GGCC 2 cut(s) 143, 149
BtgI CCRYGG 1 cut(s) 33
Csp6I GTAC 2 cut(s) 129, 185
CviAII CATG 5 cut(s) 11, 34, 200, 253, 325
CviJI RGCY 4 cut(s) 38, 143, 149, 209
CviKI_1 RGCY 4 cut(s) 38, 143, 149, 209
CviQI GTAC 2 cut(s) 129, 185
DpnI GATC 2 cut(s) 192, 213
DpnII GATC 2 cut(s) 190, 211
EaeI YGGCCR 1 cut(s) 141
Eco130I CCWWGG 2 cut(s) 33, 144
EcoT14I CCWWGG 2 cut(s) 33, 144
EcoT22I ATGCAT 1 cut(s) 163
ErhI CCWWGG 2 cut(s) 33, 144
FaeI CATG 5 cut(s) 14, 37, 203, 256, 328
FaiI YATR 9 cut(s) 12, 35, 201, 240, 254, 287, 326, 334, 341
FaqI GGGAC 1 cut(s) 263
FatI CATG 5 cut(s) 10, 33, 199, 252, 324
FspBI CTAG 1 cut(s) 39
GsuI CTGGAG 1 cut(s) 264
HaeIII GGCC 2 cut(s) 143, 149
HapII CCGG 1 cut(s) 272
Hin1II CATG 5 cut(s) 14, 37, 203, 256, 328
HpaII CCGG 1 cut(s) 272
HpyAV CCTTC 1 cut(s) 286
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4V TGCA 5 cut(s) 81, 161, 180, 217, 281
Hsp92II CATG 5 cut(s) 14, 37, 203, 256, 328
Kzo9I GATC 2 cut(s) 190, 211
LpnPI CCDG 5 cut(s) 73, 117, 180, 228, 285
LweI GCATC 2 cut(s) 19, 148
MaeI CTAG 1 cut(s) 39
MaeIII GTNAC 1 cut(s) 66
MalI GATC 2 cut(s) 192, 213
MboI GATC 2 cut(s) 190, 211
MlsI TGGCCA 1 cut(s) 143
MluCI AATT 2 cut(s) 171, 282
MluNI TGGCCA 1 cut(s) 143
MnlI CCTC 4 cut(s) 88, 239, 253, 279
Mox20I TGGCCA 1 cut(s) 143
Mph1103I ATGCAT 1 cut(s) 163
MscI TGGCCA 1 cut(s) 143
MseI TTAA 1 cut(s) 72
Msp20I TGGCCA 1 cut(s) 143
MspI CCGG 1 cut(s) 272
Mva1269I GAATGC 1 cut(s) 161
NcoI CCATGG 1 cut(s) 33
NdeII GATC 2 cut(s) 190, 211
NlaIII CATG 5 cut(s) 14, 37, 203, 256, 328
NsiI ATGCAT 1 cut(s) 163
NspI RCATGY 1 cut(s) 256
PciI ACATGT 1 cut(s) 252
PctI GAATGC 1 cut(s) 161
PflMI CCANNNNNTGG 1 cut(s) 205
PscI ACATGT 1 cut(s) 252
RsaI GTAC 2 cut(s) 130, 186
RsaNI GTAC 2 cut(s) 129, 185
SaqAI TTAA 1 cut(s) 72
Sau3AI GATC 2 cut(s) 190, 211
SetI ASST 2 cut(s) 21, 297
SfaNI GCATC 2 cut(s) 19, 148
Sse9I AATT 2 cut(s) 171, 282
SspMI CTAG 1 cut(s) 39
StyI CCWWGG 2 cut(s) 33, 144
TaaI ACNGT 1 cut(s) 184
TaqI TCGA 1 cut(s) 93
TasI AATT 2 cut(s) 171, 282
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
Van91I CCANNNNNTGG 1 cut(s) 205
XceI RCATGY 1 cut(s) 256
XspI CTAG 1 cut(s) 39
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.