Rh2AG525700

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0003208
Physical Location & Seq
Reverse (-)
75446551 .. 75448839
2289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG525700.1

Sequence Viewer

Length: 438 bp
ATGCCGAAGAACAAGGGAAAGGGAGGAAAGAACAGGAAGAGAGGAAAGAACGAGGCTGATGATGAAAAGCGTGAGCTTGTCTTCAAGGAAGATGGACAGGAGTATGCCCAAGTGCTTCGGATGCTGGGTAATGGTCGGTGCGAAGCCATGTGCATTGATGGAACCAAGCGACTTTGCCATATCCGTGGTAAGATGCACAAGAAGGTTTGGATTGCAGCTGGGGATATCATTCTTGTTGGGCTTCGTGACTATCAGGATGACAAGGCCGATGTGATTCTCAAGTACATGCCTGATGAGGCTAGGCTGCTGAAGGCTTATGGAGAGCTTCCAGAGAATACACGTCTTAACGAGGGTATTACTGGGGGTCTTGATGAGGAGGAGGAAGGTGGTGCTGATGACTACATCGAGTTTGAAGATGAAGATATTGATAAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

145

Amino Acids

16.46

Weight (kDa)

5.02

Isoelectric Point (pI)

50.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-1a PF01176 32 - 93 3e-24 Translation initiation factor 1A / IF-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 329
AfaI GTAC 1 cut(s) 284
AflIII ACRYGT 1 cut(s) 338
AgsI TTSAA 2 cut(s) 85, 413
AjiI CACGTC 1 cut(s) 341
AluBI AGCT 3 cut(s) 76, 218, 325
AluI AGCT 3 cut(s) 76, 218, 325
AoxI GGCC 1 cut(s) 264
ApeKI GCWGC 2 cut(s) 215, 304
BbsI GAAGAC 1 cut(s) 73
BbvI GCAGC 2 cut(s) 227, 291
BccI CCATC 2 cut(s) 86, 152
BfaI CTAG 1 cut(s) 300
BisI GCNGC 2 cut(s) 216, 305
BlsI GCNGC 2 cut(s) 217, 306
BmgBI CACGTC 1 cut(s) 341
BmiI GGNNCC 1 cut(s) 163
BmrI ACTGGG 1 cut(s) 369
BmsI GCATC 2 cut(s) 111, 183
BmuI ACTGGG 1 cut(s) 369
BpiI GAAGAC 1 cut(s) 73
BpuEI CTTGAG 1 cut(s) 263
BsaJI CCNNGG 1 cut(s) 184
Bse1I ACTGG 1 cut(s) 364
BseDI CCNNGG 1 cut(s) 184
BseGI GGATG 2 cut(s) 126, 262
BseNI ACTGG 1 cut(s) 364
BseRI GAGGAG 2 cut(s) 389, 392
BseXI GCAGC 2 cut(s) 227, 291
BseYI CCCAGC 2 cut(s) 124, 218
BshFI GGCC 1 cut(s) 266
BsnI GGCC 1 cut(s) 266
BspANI GGCC 1 cut(s) 266
BspLI GGNNCC 1 cut(s) 163
BsrI ACTGG 1 cut(s) 364
BssECI CCNNGG 1 cut(s) 184
Bst6I CTCTTC 1 cut(s) 32
BstDSI CCRYGG 1 cut(s) 184
BstF5I GGATG 2 cut(s) 126, 262
BstMWI GCNNNNNNNGC 1 cut(s) 121
BstNSI RCATGY 1 cut(s) 289
BstV1I GCAGC 2 cut(s) 227, 291
BstV2I GAAGAC 1 cut(s) 73
BstXI CCANNNNNNTGG 1 cut(s) 185
BsuRI GGCC 1 cut(s) 266
BtgI CCRYGG 1 cut(s) 184
BtrI CACGTC 1 cut(s) 341
BtsCI GGATG 2 cut(s) 126, 262
Csp6I GTAC 1 cut(s) 283
CviAII CATG 2 cut(s) 148, 286
CviQI GTAC 1 cut(s) 283
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Eco32I GATATC 1 cut(s) 226
Eco57I CTGAAG 1 cut(s) 329
EcoRV GATATC 1 cut(s) 226
FaeI CATG 2 cut(s) 151, 289
FaiI YATR 5 cut(s) 105, 149, 180, 287, 318
FatI CATG 2 cut(s) 147, 285
Fnu4HI GCNGC 2 cut(s) 216, 305
FokI GGATG 2 cut(s) 133, 269
Fsp4HI GCNGC 2 cut(s) 216, 305
FspBI CTAG 1 cut(s) 300
GluI GCNGC 2 cut(s) 216, 305
GsaI CCCAGC 2 cut(s) 128, 222
HaeIII GGCC 1 cut(s) 266
Hin1II CATG 2 cut(s) 151, 289
HinfI GANTC 1 cut(s) 274
Hpy188I TCNGA 1 cut(s) 120
Hpy188III TCNNGA 4 cut(s) 245, 254, 329, 368
HpyAV CCTTC 3 cut(s) 196, 304, 377
HpyCH4IV ACGT 1 cut(s) 340
HpyCH4V TGCA 3 cut(s) 153, 196, 215
HpyF10VI GCNNNNNNNGC 1 cut(s) 121
HpySE526I ACGT 1 cut(s) 340
Hsp92II CATG 2 cut(s) 151, 289
LpnPI CCDG 8 cut(s) 19, 83, 110, 204, 239, 303, 342, 345
Lsp1109I GCAGC 2 cut(s) 227, 291
LweI GCATC 2 cut(s) 111, 183
MaeI CTAG 1 cut(s) 300
MaeII ACGT 1 cut(s) 340
MaeIII GTNAC 1 cut(s) 245
MboII GAAGA 6 cut(s) 19, 49, 73, 101, 425, 431
MnlI CCTC 8 cut(s) 17, 35, 46, 289, 343, 367, 370, 373
MseI TTAA 2 cut(s) 345, 436
MslI CAYNNNNRTG 1 cut(s) 183
MspA1I CMGCKG 1 cut(s) 218
MwoI GCNNNNNNNGC 1 cut(s) 121
NlaIII CATG 2 cut(s) 151, 289
NlaIV GGNNCC 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 245
NspI RCATGY 1 cut(s) 289
PfeI GAWTC 1 cut(s) 274
PkrI GCNGC 2 cut(s) 217, 306
PspFI CCCAGC 2 cut(s) 124, 218
PspN4I GGNNCC 1 cut(s) 163
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
RseI CAYNNNNRTG 1 cut(s) 183
SaqAI TTAA 2 cut(s) 345, 436
SatI GCNGC 2 cut(s) 216, 305
SetI ASST 6 cut(s) 78, 207, 220, 327, 343, 388
SfaNI GCATC 2 cut(s) 111, 183
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
SspMI CTAG 1 cut(s) 300
TaiI ACGT 1 cut(s) 343
TaqI TCGA 1 cut(s) 405
TatI WGTACW 1 cut(s) 282
TfiI GAWTC 1 cut(s) 274
Tru1I TTAA 2 cut(s) 345, 436
Tru9I TTAA 2 cut(s) 345, 436
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 2 cut(s) 215, 304
Tsp45I GTSAC 1 cut(s) 245
TspDTI ATGAA 2 cut(s) 78, 432
TspGWI ACGGA 1 cut(s) 173
XceI RCATGY 1 cut(s) 289
XspI CTAG 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.