FvH4_6g41720

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003208
Physical Location & Seq
Reverse (-)
32727579 .. 32729076
1498 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g41720.t1

Sequence Viewer

Length: 438 bp
ATGCCGAAGAACAAGGGAAAGGGAGGAAAGAACAGGAAGAGAGGTAAGAACGAGGCTGACGATGAAAAGCGTGAGCTTGTCTTCAAGGAAGATGGACAGGAGTACGCCCAAGTGCTTCGGATGCTGGGTAATGGGCGTTGTGAAGCCATGTGCATTGATGGAACCAAGCGACTTTGCCATATCCGTGGTAAGATGCACAAGAAGGTTTGGATTGCAGCTGGGGATATCATTCTTGTTGGGCTTCGTGACTATCAGGATGACAAGGCTGATGTGATCCTTAAGTACATGCCTGATGAGGCTAGGCTGCTCAAGGCTTATGGAGAGCTTCCGGAGAACACACGTCTCAACGAGGGTATTACTGGGGGTCTTGACGAAGAGGATGAAGCTGGTGGTGATGAGTACATCGAGTTTGAGGATGAAGATATTGATAAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

146

Amino Acids

16.46

Weight (kDa)

5.02

Isoelectric Point (pI)

48.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-1a PF01176 32 - 93 3e-24 Translation initiation factor 1A / IF-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 328
AclWI GGATC 1 cut(s) 268
AfaI GTAC 3 cut(s) 104, 284, 401
AflII CTTAAG 1 cut(s) 278
AflIII ACRYGT 1 cut(s) 338
AgsI TTSAA 1 cut(s) 85
AjiI CACGTC 1 cut(s) 341
AluBI AGCT 4 cut(s) 76, 218, 325, 386
AluI AGCT 4 cut(s) 76, 218, 325, 386
Alw26I GTCTC 1 cut(s) 347
AlwI GGATC 1 cut(s) 268
Aor13HI TCCGGA 1 cut(s) 328
ApeKI GCWGC 2 cut(s) 215, 304
AsuHPI GGTGA 1 cut(s) 404
BbsI GAAGAC 1 cut(s) 73
BbvI GCAGC 2 cut(s) 227, 291
BccI CCATC 2 cut(s) 86, 152
BcoDI GTCTC 1 cut(s) 347
BfaI CTAG 1 cut(s) 300
BfrI CTTAAG 1 cut(s) 278
BisI GCNGC 2 cut(s) 216, 305
BlsI GCNGC 2 cut(s) 217, 306
BmgBI CACGTC 1 cut(s) 341
BmiI GGNNCC 1 cut(s) 163
BmrI ACTGGG 1 cut(s) 369
BmsI GCATC 2 cut(s) 111, 183
BmuI ACTGGG 1 cut(s) 369
BpiI GAAGAC 1 cut(s) 73
BpuEI CTTGAG 1 cut(s) 293
BsaJI CCNNGG 1 cut(s) 184
BsaWI WCCGGW 1 cut(s) 328
Bse1I ACTGG 1 cut(s) 364
BseAI TCCGGA 1 cut(s) 328
BseDI CCNNGG 1 cut(s) 184
BseGI GGATG 4 cut(s) 126, 262, 385, 421
BseNI ACTGG 1 cut(s) 364
BseXI GCAGC 2 cut(s) 227, 291
BseYI CCCAGC 2 cut(s) 124, 218
BsiSI CCGG 1 cut(s) 329
BsmAI GTCTC 1 cut(s) 347
BsmBI CGTCTC 1 cut(s) 347
Bsp13I TCCGGA 1 cut(s) 328
Bsp143I GATC 1 cut(s) 273
BspEI TCCGGA 1 cut(s) 328
BspLI GGNNCC 1 cut(s) 163
BspPI GGATC 1 cut(s) 268
BspTI CTTAAG 1 cut(s) 278
BsrI ACTGG 1 cut(s) 364
BssECI CCNNGG 1 cut(s) 184
BssMI GATC 1 cut(s) 273
Bst6I CTCTTC 2 cut(s) 32, 369
BstAFI CTTAAG 1 cut(s) 278
BstDSI CCRYGG 1 cut(s) 184
BstF5I GGATG 4 cut(s) 126, 262, 385, 421
BstKTI GATC 1 cut(s) 276
BstMAI GTCTC 1 cut(s) 347
BstMBI GATC 1 cut(s) 273
BstMWI GCNNNNNNNGC 1 cut(s) 121
BstNSI RCATGY 1 cut(s) 289
BstV1I GCAGC 2 cut(s) 227, 291
BstV2I GAAGAC 1 cut(s) 73
BstXI CCANNNNNNTGG 1 cut(s) 185
BtgI CCRYGG 1 cut(s) 184
BtrI CACGTC 1 cut(s) 341
BtsCI GGATG 4 cut(s) 126, 262, 385, 421
Csp6I GTAC 3 cut(s) 103, 283, 400
CviAII CATG 2 cut(s) 148, 286
CviQI GTAC 3 cut(s) 103, 283, 400
DpnI GATC 1 cut(s) 275
DpnII GATC 1 cut(s) 273
Eam1104I CTCTTC 2 cut(s) 32, 369
EarI CTCTTC 2 cut(s) 32, 369
Eco32I GATATC 1 cut(s) 226
EcoRV GATATC 1 cut(s) 226
Esp3I CGTCTC 1 cut(s) 347
FaeI CATG 2 cut(s) 151, 289
FaiI YATR 4 cut(s) 149, 180, 287, 318
FatI CATG 2 cut(s) 147, 285
Fnu4HI GCNGC 2 cut(s) 216, 305
FokI GGATG 4 cut(s) 133, 269, 392, 428
Fsp4HI GCNGC 2 cut(s) 216, 305
FspBI CTAG 1 cut(s) 300
GluI GCNGC 2 cut(s) 216, 305
GsaI CCCAGC 2 cut(s) 128, 222
HapII CCGG 1 cut(s) 329
Hin1II CATG 2 cut(s) 151, 289
HpaII CCGG 1 cut(s) 329
HphI GGTGA 1 cut(s) 404
Hpy188I TCNGA 1 cut(s) 120
Hpy188III TCNNGA 4 cut(s) 245, 254, 329, 368
HpyAV CCTTC 1 cut(s) 196
HpyCH4IV ACGT 1 cut(s) 340
HpyCH4V TGCA 3 cut(s) 153, 196, 215
HpyF10VI GCNNNNNNNGC 1 cut(s) 121
HpySE526I ACGT 1 cut(s) 340
Hsp92II CATG 2 cut(s) 151, 289
Kpn2I TCCGGA 1 cut(s) 328
Kzo9I GATC 1 cut(s) 273
LpnPI CCDG 9 cut(s) 19, 83, 110, 204, 239, 303, 342, 345, 372
Lsp1109I GCAGC 2 cut(s) 227, 291
LweI GCATC 2 cut(s) 111, 183
MaeI CTAG 1 cut(s) 300
MaeII ACGT 1 cut(s) 340
MaeIII GTNAC 1 cut(s) 245
MalI GATC 1 cut(s) 275
MboI GATC 1 cut(s) 273
MboII GAAGA 6 cut(s) 19, 49, 73, 101, 386, 431
MnlI CCTC 7 cut(s) 17, 35, 46, 289, 343, 370, 406
MroI TCCGGA 1 cut(s) 328
MseI TTAA 2 cut(s) 279, 436
MslI CAYNNNNRTG 1 cut(s) 183
MspA1I CMGCKG 1 cut(s) 218
MspCI CTTAAG 1 cut(s) 278
MspI CCGG 1 cut(s) 329
MwoI GCNNNNNNNGC 1 cut(s) 121
NdeII GATC 1 cut(s) 273
NlaIII CATG 2 cut(s) 151, 289
NlaIV GGNNCC 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 245
NspI RCATGY 1 cut(s) 289
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PkrI GCNGC 2 cut(s) 217, 306
PspFI CCCAGC 2 cut(s) 124, 218
PspN4I GGNNCC 1 cut(s) 163
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 3 cut(s) 104, 284, 401
RsaNI GTAC 3 cut(s) 103, 283, 400
RseI CAYNNNNRTG 1 cut(s) 183
SaqAI TTAA 2 cut(s) 279, 436
SatI GCNGC 2 cut(s) 216, 305
Sau3AI GATC 1 cut(s) 273
SetI ASST 7 cut(s) 46, 78, 207, 220, 327, 343, 388
SfaNI GCATC 2 cut(s) 111, 183
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 2 cut(s) 278, 308
SmoI CTYRAG 2 cut(s) 278, 308
SspMI CTAG 1 cut(s) 300
TaiI ACGT 1 cut(s) 343
TaqI TCGA 1 cut(s) 405
TatI WGTACW 2 cut(s) 282, 399
Tru1I TTAA 2 cut(s) 279, 436
Tru9I TTAA 2 cut(s) 279, 436
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 2 cut(s) 215, 304
Tsp45I GTSAC 1 cut(s) 245
TspDTI ATGAA 3 cut(s) 78, 396, 432
TspGWI ACGGA 1 cut(s) 173
Vha464I CTTAAG 1 cut(s) 278
XceI RCATGY 1 cut(s) 289
XspI CTAG 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.