Rorug02G0461500

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003208
Physical Location & Seq
Reverse (-)
59326230 .. 59327138
909 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0461500.1

Sequence Viewer

Length: 909 bp
ATGGCTGAAGAGGAGTCTCAGTCCCTCATAGAGATGACACAACGGTCTCATGTCACTAACTCATCAACATCAACCTTTCGACCATCACCATCAACCTCCCAACCATCATCATCAACCCCTCAACCTCCCATTGCCGCGCCTCAACATGTTTCTGTCCTCATGCCGGTCCAACTCACCACCGACGACATTGACGCGCCTCAACGTGTTTCTGCCCTCATGCCAGTCCAACTCACCGACGAGGACAATTATTTGCTGTGGAAATCCTTCTTCCTCCCTATCCTGGGGTCCCATAATATGTTAGATCTCGCTGAGGGAAGAGAGAAGTGTCCACCACAATTCCTGAGTAGTACAGAAGACAGAAAAAAAGTCGAAAACCCTGCCTATACCGATTGGATTAGGAGGGATCAGACATTCCTAGTGTGGATTAATGTCAGCATATCCAAGGGCCTACTACTCCGGTACGCAACAGGATGCACAAGTGGAAGAGCTCTATGGCTGACCTTCGAAAAACTATTTGCCAACAAGGCAAGAGCTCGCGTAGAACAGCTGAAGGATCAACTCAGAGACCTGAAAATGGAAAGGCCATATGAATTTAGAATGGAGGAATACATAAACTCAGCGAACCAAATCGCTGATAACCTCCATGCAATGGGGTCTCCTGTTGAGGATGATGAATTTGTTTCTTATGTACTCCGAGGACTACTTCCACAGGAATGTGATGAGTTAAAAGCCTCGATCAGGGATCGGCCTGAGCCCGTAACGCGAGAAGAATTGAATGACTTATTACTAAACCATAGATTCCCAGTCGGCCCAGTTGCCAGGATCAGCATCTTCTGTGTAGTCGTCATTTTGCTTTTGCCCTTGGGGTTATTGTTTGGTCTTCGTGGTAAAATAGCAAGCCAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

302

Amino Acids

34.21

Weight (kDa)

5.39

Isoelectric Point (pI)

61.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 117 - 265 1e-13 gag-polypeptide of LTR copia-type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 43
AccB7I CCANNNNNTGG 1 cut(s) 649
AccII CGCG 4 cut(s) 137, 194, 537, 763
AciI CCGC 1 cut(s) 135
AclWI GGATC 4 cut(s) 411, 561, 750, 830
AcsI RAATTY 2 cut(s) 590, 674
AcuI CTGAAG 2 cut(s) 27, 569
AfaI GTAC 3 cut(s) 349, 461, 690
AfiI CCNNNNNNNGG 6 cut(s) 163, 280, 281, 574, 649, 738
AflIII ACRYGT 2 cut(s) 145, 202
AgsI TTSAA 1 cut(s) 775
AjnI CCWGG 2 cut(s) 279, 818
AluBI AGCT 3 cut(s) 488, 533, 547
AluI AGCT 3 cut(s) 488, 533, 547
Alw21I GWGCWC 2 cut(s) 490, 535
Alw26I GTCTC 4 cut(s) 21, 51, 558, 660
AlwI GGATC 4 cut(s) 411, 561, 750, 830
AoxI GGCC 4 cut(s) 445, 581, 746, 808
ApoI RAATTY 2 cut(s) 590, 674
AseI ATTAAT 1 cut(s) 426
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 2 cut(s) 139, 196
AspS9I GGNCC 4 cut(s) 166, 285, 445, 809
AsuHPI GGTGA 3 cut(s) 78, 166, 223
AsuII TTCGAA 1 cut(s) 504
AvaII GGWCC 2 cut(s) 166, 285
BanII GRGCYC 3 cut(s) 490, 535, 756
BbsI GAAGAC 2 cut(s) 360, 872
Bbv12I GWGCWC 2 cut(s) 490, 535
BbvCI CCTCAGC 1 cut(s) 309
BccI CCATC 3 cut(s) 91, 97, 112
BcgI CGANNNNNNTGC 2 cut(s) 359, 393
BciT130I CCWGG 2 cut(s) 281, 820
BcoDI GTCTC 4 cut(s) 21, 51, 558, 660
BfaI CTAG 1 cut(s) 416
BglI GCCNNNNNGGC 1 cut(s) 524
BglII AGATCT 1 cut(s) 301
BisI GCNGC 1 cut(s) 135
BlsI GCNGC 1 cut(s) 136
Bme1390I CCNGG 2 cut(s) 281, 820
Bme18I GGWCC 2 cut(s) 166, 285
BmgT120I GGNCC 4 cut(s) 166, 285, 445, 809
BmiI GGNNCC 2 cut(s) 286, 287
BmrFI CCNGG 2 cut(s) 281, 820
BmrI ACTGGG 2 cut(s) 797, 806
BmsI GCATC 2 cut(s) 461, 837
BmuI ACTGGG 2 cut(s) 797, 806
BpiI GAAGAC 2 cut(s) 360, 872
Bpu10I CCTNAGC 2 cut(s) 309, 750
Bpu14I TTCGAA 1 cut(s) 504
BsaBI GATNNNNATC 1 cut(s) 827
BsaI GGTCTC 3 cut(s) 51, 558, 660
BsaJI CCNNGG 4 cut(s) 280, 441, 694, 861
BsaWI WCCGGW 1 cut(s) 456
BsaXI ACNNNNNCTCC 2 cut(s) 5, 35
Bsc4I CCNNNNNNNGG 6 cut(s) 163, 280, 281, 574, 649, 738
Bse118I RCCGGY 1 cut(s) 163
Bse1I ACTGG 3 cut(s) 221, 803, 812
Bse3DI GCAATG 2 cut(s) 129, 654
Bse8I GATNNNNATC 1 cut(s) 827
BseBI CCWGG 2 cut(s) 281, 820
BseDI CCNNGG 4 cut(s) 280, 441, 694, 861
BseGI GGATG 2 cut(s) 476, 673
BseJI GATNNNNATC 1 cut(s) 827
BseLI CCNNNNNNNGG 6 cut(s) 163, 280, 281, 574, 649, 738
BseMI GCAATG 2 cut(s) 129, 654
BseMII CTCAG 6 cut(s) 32, 300, 332, 574, 630, 741
BseNI ACTGG 3 cut(s) 221, 803, 812
BseRI GAGGAG 1 cut(s) 26
Bsh1236I CGCG 4 cut(s) 137, 194, 537, 763
BshFI GGCC 4 cut(s) 447, 583, 748, 810
BsiHKAI GWGCWC 2 cut(s) 490, 535
BsiSI CCGG 2 cut(s) 164, 457
BslFI GGGAC 2 cut(s) 7, 271
BslI CCNNNNNNNGG 6 cut(s) 163, 280, 281, 574, 649, 738
BsmAI GTCTC 4 cut(s) 21, 51, 558, 660
BsmFI GGGAC 2 cut(s) 7, 271
BsnI GGCC 4 cut(s) 447, 583, 748, 810
Bso31I GGTCTC 3 cut(s) 51, 558, 660
Bsp119I TTCGAA 1 cut(s) 504
Bsp1286I GDGCHC 3 cut(s) 490, 535, 756
Bsp143I GATC 6 cut(s) 301, 403, 553, 735, 742, 822
BspACI CCGC 1 cut(s) 135
BspANI GGCC 4 cut(s) 447, 583, 748, 810
BspCNI CTCAG 6 cut(s) 31, 301, 333, 573, 629, 742
BspFNI CGCG 4 cut(s) 137, 194, 537, 763
BspLI GGNNCC 2 cut(s) 286, 287
BspPI GGATC 4 cut(s) 411, 561, 750, 830
BspQI GCTCTTC 1 cut(s) 478
BspT104I TTCGAA 1 cut(s) 504
BspTNI GGTCTC 3 cut(s) 51, 558, 660
BsrDI GCAATG 2 cut(s) 129, 654
BsrFI RCCGGY 1 cut(s) 163
BsrI ACTGG 3 cut(s) 221, 803, 812
BssAI RCCGGY 1 cut(s) 163
BssECI CCNNGG 4 cut(s) 280, 441, 694, 861
BssMI GATC 6 cut(s) 301, 403, 553, 735, 742, 822
BssT1I CCWWGG 2 cut(s) 441, 861
Bst2UI CCWGG 2 cut(s) 281, 820
Bst4CI ACNGT 1 cut(s) 45
Bst6I CTCTTC 3 cut(s) 3, 310, 478
BstBI TTCGAA 1 cut(s) 504
BstC8I GCNNGC 2 cut(s) 535, 898
BstDEI CTNAG 6 cut(s) 18, 309, 341, 560, 616, 750
BstENI CCTNNNNNAGG 1 cut(s) 736
BstF5I GGATG 2 cut(s) 476, 673
BstFNI CGCG 4 cut(s) 137, 194, 537, 763
BstHHI GCGC 2 cut(s) 139, 196
BstKTI GATC 6 cut(s) 304, 406, 556, 738, 745, 825
BstMAI GTCTC 4 cut(s) 21, 51, 558, 660
BstMBI GATC 6 cut(s) 301, 403, 553, 735, 742, 822
BstMWI GCNNNNNNNGC 2 cut(s) 524, 760
BstNI CCWGG 2 cut(s) 281, 820
BstNSI RCATGY 1 cut(s) 149
BstSCI CCNGG 2 cut(s) 279, 818
BstUI CGCG 4 cut(s) 137, 194, 537, 763
BstV2I GAAGAC 2 cut(s) 360, 872
BstX2I RGATCY 1 cut(s) 301
BstYI RGATCY 1 cut(s) 301
BsuRI GGCC 4 cut(s) 447, 583, 748, 810
BtsCI GGATG 2 cut(s) 476, 673
Cac8I GCNNGC 2 cut(s) 535, 898
CfoI GCGC 2 cut(s) 139, 196
Cfr10I RCCGGY 1 cut(s) 163
Cfr13I GGNCC 4 cut(s) 166, 285, 445, 809
CseI GACGC 1 cut(s) 200
Csp6I GTAC 3 cut(s) 348, 460, 689
CviAII CATG 5 cut(s) 50, 146, 160, 217, 644
CviQI GTAC 3 cut(s) 348, 460, 689
DdeI CTNAG 6 cut(s) 18, 309, 341, 560, 616, 750
DpnI GATC 6 cut(s) 303, 405, 555, 737, 744, 824
DpnII GATC 6 cut(s) 301, 403, 553, 735, 742, 822
DrdI GACNNNNNNGTC 1 cut(s) 43
DseDI GACNNNNNNGTC 1 cut(s) 43
Eam1104I CTCTTC 3 cut(s) 3, 310, 478
EarI CTCTTC 3 cut(s) 3, 310, 478
Ecl136II GAGCTC 2 cut(s) 488, 533
Eco130I CCWWGG 2 cut(s) 441, 861
Eco24I GRGCYC 3 cut(s) 490, 535, 756
Eco31I GGTCTC 3 cut(s) 51, 558, 660
Eco47I GGWCC 2 cut(s) 166, 285
Eco53kI GAGCTC 2 cut(s) 488, 533
Eco57I CTGAAG 2 cut(s) 27, 569
EcoICRI GAGCTC 2 cut(s) 488, 533
EcoNI CCTNNNNNAGG 1 cut(s) 736
EcoO109I RGGNCCY 2 cut(s) 285, 445
EcoRII CCWGG 2 cut(s) 279, 818
EcoT14I CCWWGG 2 cut(s) 441, 861
EcoT38I GRGCYC 3 cut(s) 490, 535, 756
ErhI CCWWGG 2 cut(s) 441, 861
FaeI CATG 5 cut(s) 53, 149, 163, 220, 647
FaqI GGGAC 2 cut(s) 7, 271
FatI CATG 5 cut(s) 49, 145, 159, 216, 643
FauNDI CATATG 1 cut(s) 586
Fnu4HI GCNGC 1 cut(s) 135
FokI GGATG 2 cut(s) 483, 680
FriOI GRGCYC 3 cut(s) 490, 535, 756
Fsp4HI GCNGC 1 cut(s) 135
FspBI CTAG 1 cut(s) 416
GlaI GCGC 2 cut(s) 138, 195
GluI GCNGC 1 cut(s) 135
HaeIII GGCC 4 cut(s) 447, 583, 748, 810
HapII CCGG 2 cut(s) 164, 457
HgaI GACGC 1 cut(s) 200
HhaI GCGC 2 cut(s) 139, 196
Hin1II CATG 5 cut(s) 53, 149, 163, 220, 647
Hin6I GCGC 2 cut(s) 137, 194
HinP1I GCGC 2 cut(s) 137, 194
HinfI GANTC 2 cut(s) 14, 798
HpaII CCGG 2 cut(s) 164, 457
HphI GGTGA 3 cut(s) 78, 166, 223
Hpy166II GTNNAC 1 cut(s) 329
Hpy188I TCNGA 3 cut(s) 408, 563, 695
Hpy188III TCNNGA 1 cut(s) 340
Hpy8I GTNNAC 1 cut(s) 329
Hpy99I CGWCG 2 cut(s) 185, 239
HpyAV CCTTC 3 cut(s) 274, 511, 544
HpyCH4III ACNGT 1 cut(s) 45
HpyCH4IV ACGT 1 cut(s) 202
HpyCH4V TGCA 2 cut(s) 474, 647
HpyF10VI GCNNNNNNNGC 2 cut(s) 524, 760
HpyF3I CTNAG 6 cut(s) 18, 309, 341, 560, 616, 750
HpySE526I ACGT 1 cut(s) 202
Hsp92II CATG 5 cut(s) 53, 149, 163, 220, 647
HspAI GCGC 2 cut(s) 137, 194
KflI GGGWCCC 1 cut(s) 285
Kzo9I GATC 6 cut(s) 301, 403, 553, 735, 742, 822
LguI GCTCTTC 1 cut(s) 478
LweI GCATC 2 cut(s) 461, 837
MaeI CTAG 1 cut(s) 416
MaeII ACGT 1 cut(s) 202
MaeIII GTNAC 2 cut(s) 52, 757
MalI GATC 6 cut(s) 303, 405, 555, 737, 744, 824
MboI GATC 6 cut(s) 301, 403, 553, 735, 742, 822
MboII GAAGA 8 cut(s) 20, 259, 327, 365, 495, 779, 823, 872
MflI RGATCY 1 cut(s) 301
MhlI GDGCHC 3 cut(s) 490, 535, 756
MluCI AATT 6 cut(s) 244, 335, 590, 674, 770, 904
MlyI GAGTC 1 cut(s) 23
MmeI TCCRAC 2 cut(s) 193, 250
MroXI GAANNNNTTC 1 cut(s) 263
MseI TTAA 2 cut(s) 426, 725
MslI CAYNNNNRTG 2 cut(s) 32, 712
MspA1I CMGCKG 1 cut(s) 547
MspI CCGG 2 cut(s) 164, 457
MspR9I CCNGG 2 cut(s) 281, 820
MvaI CCWGG 2 cut(s) 281, 820
MvnI CGCG 4 cut(s) 137, 194, 537, 763
MwoI GCNNNNNNNGC 2 cut(s) 524, 760
NdeI CATATG 1 cut(s) 586
NdeII GATC 6 cut(s) 301, 403, 553, 735, 742, 822
NlaIII CATG 5 cut(s) 53, 149, 163, 220, 647
NlaIV GGNNCC 2 cut(s) 286, 287
NmuCI GTSAC 1 cut(s) 52
NspI RCATGY 1 cut(s) 149
NspV TTCGAA 1 cut(s) 504
PciI ACATGT 1 cut(s) 145
PciSI GCTCTTC 1 cut(s) 478
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 1 cut(s) 798
PflMI CCANNNNNTGG 1 cut(s) 649
PkrI GCNGC 1 cut(s) 136
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
PpuMI RGGWCCY 1 cut(s) 285
PscI ACATGT 1 cut(s) 145
PshBI ATTAAT 1 cut(s) 426
Psp124BI GAGCTC 2 cut(s) 490, 535
Psp5II RGGWCCY 1 cut(s) 285
Psp6I CCWGG 2 cut(s) 279, 818
PspGI CCWGG 2 cut(s) 279, 818
PspN4I GGNNCC 2 cut(s) 286, 287
PspPI GGNCC 4 cut(s) 166, 285, 445, 809
PspPPI RGGWCCY 1 cut(s) 285
PsuI RGATCY 1 cut(s) 301
PvuII CAGCTG 1 cut(s) 547
RsaI GTAC 3 cut(s) 349, 461, 690
RsaNI GTAC 3 cut(s) 348, 460, 689
RseI CAYNNNNRTG 2 cut(s) 32, 712
SacI GAGCTC 2 cut(s) 490, 535
SapI GCTCTTC 1 cut(s) 478
SaqAI TTAA 2 cut(s) 426, 725
SatI GCNGC 1 cut(s) 135
Sau3AI GATC 6 cut(s) 301, 403, 553, 735, 742, 822
Sau96I GGNCC 4 cut(s) 166, 285, 445, 809
SchI GAGTC 1 cut(s) 23
ScrFI CCNGG 2 cut(s) 281, 820
SduI GDGCHC 3 cut(s) 490, 535, 756
SfaNI GCATC 2 cut(s) 461, 837
SfuI TTCGAA 1 cut(s) 504
SinI GGWCC 2 cut(s) 166, 285
SmiMI CAYNNNNRTG 2 cut(s) 32, 712
Sse9I AATT 6 cut(s) 244, 335, 590, 674, 770, 904
SsiI CCGC 1 cut(s) 135
SspMI CTAG 1 cut(s) 416
SstI GAGCTC 2 cut(s) 490, 535
StyD4I CCNGG 2 cut(s) 279, 818
StyI CCWWGG 2 cut(s) 441, 861
TaaI ACNGT 1 cut(s) 45
TaiI ACGT 1 cut(s) 205
TaqI TCGA 4 cut(s) 79, 369, 504, 734
TasI AATT 6 cut(s) 244, 335, 590, 674, 770, 904
TatI WGTACW 2 cut(s) 347, 688
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 1 cut(s) 798
Tru1I TTAA 2 cut(s) 426, 725
Tru9I TTAA 2 cut(s) 426, 725
TseFI GTSAC 1 cut(s) 52
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 2 cut(s) 603, 687
Van91I CCANNNNNTGG 1 cut(s) 649
VpaK11BI GGWCC 2 cut(s) 166, 285
VspI ATTAAT 1 cut(s) 426
XagI CCTNNNNNAGG 1 cut(s) 736
XapI RAATTY 2 cut(s) 590, 674
XceI RCATGY 1 cut(s) 149
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.