FvH4_6g30180

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003208
Physical Location & Seq
Reverse (-)
23321239 .. 23322327
1089 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g30180.t1

Sequence Viewer

Length: 432 bp
ATGCCAAAGAACAAGGGAAAGGGAGGAAAGAACAGGAAGAGAGGTAAGAACGAAGCTGACGACGAAAAGCGTGAGCTTGTGTTCAAGGAAGATGGACAGGAATATGCCCAAGTGCTTCGGATGCTGGGCAATGGTCGTTGCGAAGCAATGTGCATGGATGCAATCAAGAGACTTTGCCATATCCGTGGTAAGATGCACAAGAAGGTTTGGATTGCAGCTGGGGATATCATTCTTGTTGGCCTTCGTGACTATCAGGATGACAAGGCTGATGTGATTCTCAAGTACATGTCTGACGAGGCTAGGCTGCTCAAGGCTTATGGAGAGCTTCCAGAAAACACACGTCTTAACGAGGGTGTGATTGTGGACGAACACGATGATGATGCTCCGGATGACTACATCGAGTTTGAGGATGAAGATATTGATAAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

144

Amino Acids

16.44

Weight (kDa)

5.08

Isoelectric Point (pI)

46.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-1a PF01176 32 - 93 1.7e-23 Translation initiation factor 1A / IF-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 385
AfaI GTAC 1 cut(s) 284
AflIII ACRYGT 2 cut(s) 285, 338
AgsI TTSAA 1 cut(s) 85
AjiI CACGTC 1 cut(s) 341
AluBI AGCT 4 cut(s) 56, 76, 218, 325
AluI AGCT 4 cut(s) 56, 76, 218, 325
Alw26I GTCTC 1 cut(s) 163
Aor13HI TCCGGA 1 cut(s) 385
AoxI GGCC 1 cut(s) 238
ApeKI GCWGC 2 cut(s) 215, 304
BbvI GCAGC 2 cut(s) 227, 291
BccI CCATC 1 cut(s) 86
BcgI CGANNNNNNTGC 2 cut(s) 362, 396
BcoDI GTCTC 1 cut(s) 163
BfaI CTAG 1 cut(s) 300
BisI GCNGC 2 cut(s) 216, 305
BlsI GCNGC 2 cut(s) 217, 306
BmgBI CACGTC 1 cut(s) 341
BmsI GCATC 4 cut(s) 111, 148, 183, 370
BpuEI CTTGAG 2 cut(s) 263, 293
BsaJI CCNNGG 1 cut(s) 184
BsaWI WCCGGW 1 cut(s) 385
Bse3DI GCAATG 2 cut(s) 136, 153
BseAI TCCGGA 1 cut(s) 385
BseDI CCNNGG 1 cut(s) 184
BseGI GGATG 5 cut(s) 126, 163, 262, 394, 415
BseMI GCAATG 2 cut(s) 136, 153
BseXI GCAGC 2 cut(s) 227, 291
BseYI CCCAGC 2 cut(s) 124, 218
BshFI GGCC 1 cut(s) 240
BsiSI CCGG 1 cut(s) 386
BsmAI GTCTC 1 cut(s) 163
BsnI GGCC 1 cut(s) 240
Bsp13I TCCGGA 1 cut(s) 385
BspANI GGCC 1 cut(s) 240
BspEI TCCGGA 1 cut(s) 385
BsrDI GCAATG 2 cut(s) 136, 153
BssECI CCNNGG 1 cut(s) 184
Bst6I CTCTTC 1 cut(s) 32
BstDSI CCRYGG 1 cut(s) 184
BstF5I GGATG 5 cut(s) 126, 163, 262, 394, 415
BstMAI GTCTC 1 cut(s) 163
BstMWI GCNNNNNNNGC 1 cut(s) 121
BstNSI RCATGY 1 cut(s) 289
BstV1I GCAGC 2 cut(s) 227, 291
BstXI CCANNNNNNTGG 1 cut(s) 185
BsuRI GGCC 1 cut(s) 240
BtgI CCRYGG 1 cut(s) 184
BtrI CACGTC 1 cut(s) 341
BtsCI GGATG 5 cut(s) 126, 163, 262, 394, 415
Csp6I GTAC 1 cut(s) 283
CviAII CATG 2 cut(s) 154, 286
CviJI RGCY 9 cut(s) 56, 76, 218, 240, 266, 299, 304, 314, 325
CviKI_1 RGCY 9 cut(s) 56, 76, 218, 240, 266, 299, 304, 314, 325
CviQI GTAC 1 cut(s) 283
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Eco32I GATATC 1 cut(s) 226
EcoRV GATATC 1 cut(s) 226
FaeI CATG 2 cut(s) 157, 289
FaiI YATR 5 cut(s) 105, 155, 180, 287, 318
FatI CATG 2 cut(s) 153, 285
Fnu4HI GCNGC 2 cut(s) 216, 305
FokI GGATG 5 cut(s) 133, 170, 269, 401, 422
Fsp4HI GCNGC 2 cut(s) 216, 305
FspBI CTAG 1 cut(s) 300
GluI GCNGC 2 cut(s) 216, 305
GsaI CCCAGC 2 cut(s) 128, 222
HaeIII GGCC 1 cut(s) 240
HapII CCGG 1 cut(s) 386
Hin1II CATG 2 cut(s) 157, 289
HinfI GANTC 1 cut(s) 274
HpaII CCGG 1 cut(s) 386
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 2 cut(s) 120, 292
Hpy188III TCNNGA 5 cut(s) 166, 245, 254, 329, 386
Hpy8I GTNNAC 1 cut(s) 364
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 2 cut(s) 196, 251
HpyCH4IV ACGT 1 cut(s) 340
HpyCH4V TGCA 4 cut(s) 153, 161, 196, 215
HpyF10VI GCNNNNNNNGC 1 cut(s) 121
HpySE526I ACGT 1 cut(s) 340
Hsp92II CATG 2 cut(s) 157, 289
Kpn2I TCCGGA 1 cut(s) 385
LmnI GCTCC 1 cut(s) 388
LpnPI CCDG 7 cut(s) 19, 83, 110, 204, 239, 342, 399
Lsp1109I GCAGC 2 cut(s) 227, 291
LweI GCATC 4 cut(s) 111, 148, 183, 370
MaeI CTAG 1 cut(s) 300
MaeII ACGT 1 cut(s) 340
MaeIII GTNAC 1 cut(s) 245
MboII GAAGA 3 cut(s) 49, 101, 425
MnlI CCTC 5 cut(s) 17, 35, 289, 343, 400
MroI TCCGGA 1 cut(s) 385
MseI TTAA 2 cut(s) 345, 430
MslI CAYNNNNRTG 2 cut(s) 183, 375
MspA1I CMGCKG 1 cut(s) 218
MspI CCGG 1 cut(s) 386
MwoI GCNNNNNNNGC 1 cut(s) 121
NlaIII CATG 2 cut(s) 157, 289
NmuCI GTSAC 1 cut(s) 245
NspI RCATGY 1 cut(s) 289
PciI ACATGT 1 cut(s) 285
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PfeI GAWTC 1 cut(s) 274
PkrI GCNGC 2 cut(s) 217, 306
PscI ACATGT 1 cut(s) 285
PspFI CCCAGC 2 cut(s) 124, 218
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
RseI CAYNNNNRTG 2 cut(s) 183, 375
SaqAI TTAA 2 cut(s) 345, 430
SatI GCNGC 2 cut(s) 216, 305
SetI ASST 7 cut(s) 46, 58, 78, 207, 220, 327, 343
SfaNI GCATC 4 cut(s) 111, 148, 183, 370
SmiMI CAYNNNNRTG 2 cut(s) 183, 375
SmlI CTYRAG 2 cut(s) 278, 308
SmoI CTYRAG 2 cut(s) 278, 308
SspMI CTAG 1 cut(s) 300
TaiI ACGT 1 cut(s) 343
TaqI TCGA 1 cut(s) 399
TatI WGTACW 1 cut(s) 282
TfiI GAWTC 1 cut(s) 274
Tru1I TTAA 2 cut(s) 345, 430
Tru9I TTAA 2 cut(s) 345, 430
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 2 cut(s) 215, 304
Tsp45I GTSAC 1 cut(s) 245
TspDTI ATGAA 1 cut(s) 426
TspGWI ACGGA 1 cut(s) 173
XceI RCATGY 1 cut(s) 289
XspI CTAG 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.