MD17G1109000.v1.1

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0003208
Physical Location & Seq
Forward (+)
9340432 .. 9341148
717 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1109000.v1.1.491

Sequence Viewer

Length: 441 bp
ATGCCAAAGAACAAGGGTAAGGGAGGAAAGAACAGGAAGAGAGGTAAGAACGAAGCTGATGACGAAAAGCGTGAGCTTGTGTTTAAGGAAGATGGACAGGAGTATGCCCAAGTGCTCCGAATGCTTGGTAACGGTCGGTGTGAAGCTATGTGCATTGATGGGACCAAGCGACTTTGTCACATCCGTGGTAAGATGCACAAGAAGGTTTGGATTGCAGCTGGTGATATCATTCTTGTCGGCCTTCGTGACTATCAGGACGACAAAGCTGATGTGATCCTCAAGTACATGCCTGATGAGGCTAGGCTCCTGAAGGCATATGGAGAGCTTCCAGAGAATACACGTCTCAACGAGGGTATTGCTGGGGGTATTGATGATGAAGAAACCGAAGGTGGTGTTGACGACTATATTGAGTTTGAGGATGAAGATATCGACAAGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

147

Amino Acids

16.55

Weight (kDa)

5.01

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-1a PF01176 32 - 93 3.1e-24 Translation initiation factor 1A / IF-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 268
AcuI CTGAAG 1 cut(s) 329
AfaI GTAC 1 cut(s) 284
AflIII ACRYGT 1 cut(s) 338
AjiI CACGTC 1 cut(s) 341
AleI CACNNNNGTG 1 cut(s) 183
AluBI AGCT 6 cut(s) 56, 76, 146, 218, 266, 325
AluI AGCT 6 cut(s) 56, 76, 146, 218, 266, 325
Alw21I GWGCWC 1 cut(s) 117
Alw26I GTCTC 1 cut(s) 347
AlwI GGATC 1 cut(s) 268
AoxI GGCC 1 cut(s) 238
ApeKI GCWGC 1 cut(s) 215
AspS9I GGNCC 1 cut(s) 162
AsuHPI GGTGA 1 cut(s) 233
AvaII GGWCC 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 1 cut(s) 227
BccI CCATC 2 cut(s) 86, 152
BcgI CGANNNNNNTGC 2 cut(s) 338, 372
BcoDI GTCTC 1 cut(s) 347
BfaI CTAG 1 cut(s) 300
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
Bme18I GGWCC 1 cut(s) 162
BmgBI CACGTC 1 cut(s) 341
BmgT120I GGNCC 1 cut(s) 162
BmiI GGNNCC 2 cut(s) 163, 305
BmsI GCATC 1 cut(s) 183
BpuEI CTTGAG 1 cut(s) 263
BsaJI CCNNGG 1 cut(s) 184
BseDI CCNNGG 1 cut(s) 184
BseGI GGATG 2 cut(s) 180, 424
BseXI GCAGC 1 cut(s) 227
BseYI CCCAGC 1 cut(s) 359
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 1 cut(s) 240
BsiEI CGRYCG 1 cut(s) 136
BsiHKAI GWGCWC 1 cut(s) 117
BslFI GGGAC 1 cut(s) 175
BsmAI GTCTC 1 cut(s) 347
BsmBI CGTCTC 1 cut(s) 347
BsmFI GGGAC 1 cut(s) 175
BsmI GAATGC 1 cut(s) 126
BsnI GGCC 1 cut(s) 240
Bsp1286I GDGCHC 1 cut(s) 117
Bsp143I GATC 1 cut(s) 273
BspANI GGCC 1 cut(s) 240
BspLI GGNNCC 2 cut(s) 163, 305
BspPI GGATC 1 cut(s) 268
BssECI CCNNGG 1 cut(s) 184
BssMI GATC 1 cut(s) 273
Bst4CI ACNGT 1 cut(s) 134
Bst6I CTCTTC 1 cut(s) 32
BstDSI CCRYGG 1 cut(s) 184
BstF5I GGATG 2 cut(s) 180, 424
BstKTI GATC 1 cut(s) 276
BstMAI GTCTC 1 cut(s) 347
BstMBI GATC 1 cut(s) 273
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 1 cut(s) 121
BstNSI RCATGY 1 cut(s) 289
BstV1I GCAGC 1 cut(s) 227
BsuRI GGCC 1 cut(s) 240
BtgI CCRYGG 1 cut(s) 184
BtrI CACGTC 1 cut(s) 341
BtsCI GGATG 2 cut(s) 180, 424
Cfr13I GGNCC 1 cut(s) 162
Csp6I GTAC 1 cut(s) 283
CviAII CATG 1 cut(s) 286
CviJI RGCY 9 cut(s) 56, 76, 146, 218, 240, 266, 299, 304, 325
CviKI_1 RGCY 9 cut(s) 56, 76, 146, 218, 240, 266, 299, 304, 325
CviQI GTAC 1 cut(s) 283
DpnI GATC 1 cut(s) 275
DpnII GATC 1 cut(s) 273
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Eco32I GATATC 2 cut(s) 226, 427
Eco47I GGWCC 1 cut(s) 162
Eco57I CTGAAG 1 cut(s) 329
EcoRV GATATC 2 cut(s) 226, 427
Esp3I CGTCTC 1 cut(s) 347
FaeI CATG 1 cut(s) 289
FaiI YATR 7 cut(s) 105, 149, 287, 316, 318, 405, 439
FaqI GGGAC 1 cut(s) 175
FatI CATG 1 cut(s) 285
FauNDI CATATG 1 cut(s) 316
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 2 cut(s) 167, 431
Fsp4HI GCNGC 1 cut(s) 216
FspBI CTAG 1 cut(s) 300
GluI GCNGC 1 cut(s) 216
GsaI CCCAGC 1 cut(s) 363
HaeIII GGCC 1 cut(s) 240
Hin1II CATG 1 cut(s) 289
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 1 cut(s) 397
Hpy188I TCNGA 1 cut(s) 119
Hpy188III TCNNGA 4 cut(s) 245, 254, 307, 329
Hpy8I GTNNAC 1 cut(s) 397
HpyAV CCTTC 4 cut(s) 196, 251, 304, 380
HpyCH4III ACNGT 1 cut(s) 134
HpyCH4IV ACGT 1 cut(s) 340
HpyCH4V TGCA 3 cut(s) 153, 196, 215
HpyF10VI GCNNNNNNNGC 1 cut(s) 121
HpySE526I ACGT 1 cut(s) 340
Hsp92II CATG 1 cut(s) 289
Kzo9I GATC 1 cut(s) 273
LmnI GCTCC 2 cut(s) 120, 309
LpnPI CCDG 8 cut(s) 19, 83, 204, 239, 303, 320, 342, 345
Lsp1109I GCAGC 1 cut(s) 227
LweI GCATC 1 cut(s) 183
MaeI CTAG 1 cut(s) 300
MaeII ACGT 1 cut(s) 340
MaeIII GTNAC 3 cut(s) 128, 176, 245
MalI GATC 1 cut(s) 275
MboI GATC 1 cut(s) 273
MboII GAAGA 4 cut(s) 49, 101, 389, 434
MhlI GDGCHC 1 cut(s) 117
MnlI CCTC 6 cut(s) 17, 35, 287, 289, 343, 409
MseI TTAA 1 cut(s) 84
MslI CAYNNNNRTG 1 cut(s) 183
MspA1I CMGCKG 1 cut(s) 218
Mva1269I GAATGC 1 cut(s) 126
MwoI GCNNNNNNNGC 1 cut(s) 121
NdeI CATATG 1 cut(s) 316
NdeII GATC 1 cut(s) 273
NlaIII CATG 1 cut(s) 289
NlaIV GGNNCC 2 cut(s) 163, 305
NmuCI GTSAC 2 cut(s) 176, 245
NspI RCATGY 1 cut(s) 289
OliI CACNNNNGTG 1 cut(s) 183
PctI GAATGC 1 cut(s) 126
PflFI GACNNNGTC 1 cut(s) 174
PkrI GCNGC 1 cut(s) 217
PspFI CCCAGC 1 cut(s) 359
PspN4I GGNNCC 2 cut(s) 163, 305
PspPI GGNCC 1 cut(s) 162
PsyI GACNNNGTC 1 cut(s) 174
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
RseI CAYNNNNRTG 1 cut(s) 183
SaqAI TTAA 1 cut(s) 84
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 1 cut(s) 273
Sau96I GGNCC 1 cut(s) 162
SduI GDGCHC 1 cut(s) 117
SfaNI GCATC 1 cut(s) 183
SinI GGWCC 1 cut(s) 162
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
SspMI CTAG 1 cut(s) 300
TaaI ACNGT 1 cut(s) 134
TaiI ACGT 1 cut(s) 343
TaqI TCGA 1 cut(s) 429
TatI WGTACW 1 cut(s) 282
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TseFI GTSAC 2 cut(s) 176, 245
TseI GCWGC 1 cut(s) 215
Tsp45I GTSAC 2 cut(s) 176, 245
TspDTI ATGAA 2 cut(s) 390, 435
TspGWI ACGGA 1 cut(s) 173
Tth111I GACNNNGTC 1 cut(s) 174
VpaK11BI GGWCC 1 cut(s) 162
XceI RCATGY 1 cut(s) 289
XspI CTAG 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.