Rroxscaffold_2G00108330

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003208
Physical Location & Seq
Reverse (-)
32304937 .. 32305910
974 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00108330.1

Sequence Viewer

Length: 438 bp
ATGCCAAAGAACAAGGGAAAGGGAGGAAAGAACAGGAAGAGAGGTAAGAACGAGGCTGACGACGAAAAGCGTGAGCTTGTCTTCAAGGAAGATGGACAGGAGTATGCCCAAGTGCTTCGCATGCTGGGTAATGGTCGGTGCGAAGCCATGTGCATTGATGGACCCAAGCGACTTTGCCATATCCGTGGTAAGATGCACAAGAAGGTTTGGATTGCAGCTGGGGATATCATTCTTGTTGGCCTTCGTGACTATCAGGATGACAAGGCTGATGTCATTCTCAAGTACATGCCTGATGAGGCTAGGCTGCTCAAGGCTTATGGAGAGCTTTCAGAAAATGTACGTCTTAACGAGGGTGTTGGTATTAGTCTTGAAGATGGCAATGAAGGTGCTGCTGATGACTACCTCGAGTTTGAGGATGAAGACATTGATAAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

145

Amino Acids

16.4

Weight (kDa)

5.21

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-1a PF01176 32 - 93 6.5e-24 Translation initiation factor 1A / IF-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 2 cut(s) 284, 339
AgsI TTSAA 2 cut(s) 85, 371
AluBI AGCT 3 cut(s) 76, 218, 325
AluI AGCT 3 cut(s) 76, 218, 325
Ama87I CYCGRG 1 cut(s) 404
AoxI GGCC 1 cut(s) 238
ApeKI GCWGC 3 cut(s) 215, 304, 389
AspS9I GGNCC 1 cut(s) 161
AvaI CYCGRG 1 cut(s) 404
AvaII GGWCC 1 cut(s) 161
BbsI GAAGAC 2 cut(s) 73, 426
BbvI GCAGC 3 cut(s) 227, 291, 376
BccI CCATC 3 cut(s) 86, 152, 368
BfaI CTAG 1 cut(s) 300
BisI GCNGC 3 cut(s) 216, 305, 390
BlsI GCNGC 3 cut(s) 217, 306, 391
Bme18I GGWCC 1 cut(s) 161
BmeT110I CYCGRG 1 cut(s) 404
BmgT120I GGNCC 1 cut(s) 161
BmiI GGNNCC 1 cut(s) 163
BmsI GCATC 1 cut(s) 183
BpiI GAAGAC 2 cut(s) 73, 426
BpuEI CTTGAG 2 cut(s) 263, 293
BsaJI CCNNGG 1 cut(s) 184
Bse3DI GCAATG 1 cut(s) 385
BseDI CCNNGG 1 cut(s) 184
BseGI GGATG 2 cut(s) 262, 421
BseMI GCAATG 1 cut(s) 385
BseXI GCAGC 3 cut(s) 227, 291, 376
BseYI CCCAGC 2 cut(s) 124, 218
BshFI GGCC 1 cut(s) 240
BsiHKCI CYCGRG 1 cut(s) 404
BsnI GGCC 1 cut(s) 240
BsoBI CYCGRG 1 cut(s) 404
BspANI GGCC 1 cut(s) 240
BspLI GGNNCC 1 cut(s) 163
BsrDI GCAATG 1 cut(s) 385
BssECI CCNNGG 1 cut(s) 184
Bst6I CTCTTC 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 122
BstDSI CCRYGG 1 cut(s) 184
BstF5I GGATG 2 cut(s) 262, 421
BstMWI GCNNNNNNNGC 1 cut(s) 121
BstNSI RCATGY 2 cut(s) 124, 289
BstV1I GCAGC 3 cut(s) 227, 291, 376
BstV2I GAAGAC 2 cut(s) 73, 426
BstXI CCANNNNNNTGG 1 cut(s) 185
BsuRI GGCC 1 cut(s) 240
BtgI CCRYGG 1 cut(s) 184
BtsCI GGATG 2 cut(s) 262, 421
Cac8I GCNNGC 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 161
Csp6I GTAC 2 cut(s) 283, 338
CviAII CATG 3 cut(s) 121, 148, 286
CviQI GTAC 2 cut(s) 283, 338
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Eco32I GATATC 1 cut(s) 226
Eco47I GGWCC 1 cut(s) 161
Eco88I CYCGRG 1 cut(s) 404
EcoRV GATATC 1 cut(s) 226
FaeI CATG 3 cut(s) 124, 151, 289
FaiI YATR 6 cut(s) 105, 122, 149, 180, 287, 318
FatI CATG 3 cut(s) 120, 147, 285
Fnu4HI GCNGC 3 cut(s) 216, 305, 390
FokI GGATG 2 cut(s) 269, 428
Fsp4HI GCNGC 3 cut(s) 216, 305, 390
FspBI CTAG 1 cut(s) 300
GluI GCNGC 3 cut(s) 216, 305, 390
GsaI CCCAGC 2 cut(s) 128, 222
HaeIII GGCC 1 cut(s) 240
Hin1II CATG 3 cut(s) 124, 151, 289
Hpy188I TCNGA 1 cut(s) 331
Hpy188III TCNNGA 3 cut(s) 245, 254, 368
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 3 cut(s) 196, 251, 377
HpyCH4IV ACGT 1 cut(s) 340
HpyCH4V TGCA 3 cut(s) 153, 196, 215
HpyF10VI GCNNNNNNNGC 1 cut(s) 121
HpySE526I ACGT 1 cut(s) 340
Hsp92II CATG 3 cut(s) 124, 151, 289
LpnPI CCDG 6 cut(s) 19, 83, 110, 204, 239, 303
Lsp1109I GCAGC 3 cut(s) 227, 291, 376
LweI GCATC 1 cut(s) 183
MaeI CTAG 1 cut(s) 300
MaeII ACGT 1 cut(s) 340
MaeIII GTNAC 1 cut(s) 245
MboII GAAGA 5 cut(s) 49, 73, 101, 383, 431
MnlI CCTC 7 cut(s) 17, 35, 46, 289, 343, 406, 413
MseI TTAA 2 cut(s) 345, 436
MslI CAYNNNNRTG 1 cut(s) 183
MspA1I CMGCKG 1 cut(s) 218
MwoI GCNNNNNNNGC 1 cut(s) 121
NlaIII CATG 3 cut(s) 124, 151, 289
NlaIV GGNNCC 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 245
NspI RCATGY 2 cut(s) 124, 289
PaeI GCATGC 1 cut(s) 124
PaeR7I CTCGAG 1 cut(s) 404
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PkrI GCNGC 3 cut(s) 217, 306, 391
PspFI CCCAGC 2 cut(s) 124, 218
PspN4I GGNNCC 1 cut(s) 163
PspPI GGNCC 1 cut(s) 161
PspXI VCTCGAGB 1 cut(s) 404
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 2 cut(s) 284, 339
RsaNI GTAC 2 cut(s) 283, 338
RseI CAYNNNNRTG 1 cut(s) 183
SaqAI TTAA 2 cut(s) 345, 436
SatI GCNGC 3 cut(s) 216, 305, 390
Sau96I GGNCC 1 cut(s) 161
SetI ASST 8 cut(s) 46, 78, 207, 220, 327, 343, 388, 405
SfaNI GCATC 1 cut(s) 183
Sfr274I CTCGAG 1 cut(s) 404
SinI GGWCC 1 cut(s) 161
SlaI CTCGAG 1 cut(s) 404
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 3 cut(s) 278, 308, 404
SmoI CTYRAG 3 cut(s) 278, 308, 404
SphI GCATGC 1 cut(s) 124
SspMI CTAG 1 cut(s) 300
TaiI ACGT 1 cut(s) 343
TaqI TCGA 1 cut(s) 405
TatI WGTACW 1 cut(s) 282
Tru1I TTAA 2 cut(s) 345, 436
Tru9I TTAA 2 cut(s) 345, 436
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 3 cut(s) 215, 304, 389
Tsp45I GTSAC 1 cut(s) 245
TspDTI ATGAA 2 cut(s) 396, 432
TspGWI ACGGA 1 cut(s) 173
VpaK11BI GGWCC 1 cut(s) 161
XceI RCATGY 2 cut(s) 124, 289
XhoI CTCGAG 1 cut(s) 404
XspI CTAG 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.