AT5G35680

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003208
Physical Location & Seq
Reverse (-)
13857921 .. 13859085
1165 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G35680.2

Sequence Viewer

Length: 438 bp
ATGCCGAAGAACAAAGGAAAGGGAGGAAAGAACAGGAAGAGAGGAAAGAACGAAGCTGATGACGAAAAGCGAGAGCTTATATTCAAAGAAGATGGACAAGAATACGCACAAGTTCTTCGTATGCTCGGTAACGGTCGATGCGACGTTATGTGCATTGACGGTGTTAAGCGTCTTTGCCATATCCGTGGTAAGATGCATAAGAAGGTTTGGATTGCTGCTGGTGACATCATACTTGTTGGTCTAAGAGATTACCAGGATGATAAAGCTGATGTTATACTCAAGTATATGTCTGATGAGGCTAGGCTTCTTAAGGCGTATGGTGAGCTTCCGGAGAATACTCGTCTTAACGAAGGTATTGTTGGTGATCTTGATGATGATGATGATGTTGTTGGTGAAGATTACATTGAGTTTGAGGATGAGGATATTGATAGGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

145

Amino Acids

16.59

Weight (kDa)

4.89

Isoelectric Point (pI)

45.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-1a PF01176 32 - 93 1.2e-24 Translation initiation factor 1A / IF-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 328
AflII CTTAAG 1 cut(s) 308
AgsI TTSAA 1 cut(s) 85
AjnI CCWGG 1 cut(s) 252
AjuI GAANNNNNNNTTGG 2 cut(s) 342, 374
AluBI AGCT 4 cut(s) 56, 76, 266, 325
AluI AGCT 4 cut(s) 56, 76, 266, 325
Aor13HI TCCGGA 1 cut(s) 328
ApeKI GCWGC 1 cut(s) 215
AsuHPI GGTGA 4 cut(s) 233, 332, 374, 404
BbvI GCAGC 1 cut(s) 202
BccI CCATC 1 cut(s) 86
BciT130I CCWGG 1 cut(s) 254
BfaI CTAG 1 cut(s) 300
BfrI CTTAAG 1 cut(s) 308
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
Bme1390I CCNGG 1 cut(s) 254
BmrFI CCNGG 1 cut(s) 254
BmsI GCATC 2 cut(s) 128, 183
BpuEI CTTGAG 1 cut(s) 263
BsaJI CCNNGG 1 cut(s) 184
BsaWI WCCGGW 1 cut(s) 328
BseAI TCCGGA 1 cut(s) 328
BseBI CCWGG 1 cut(s) 254
BseDI CCNNGG 1 cut(s) 184
BseGI GGATG 2 cut(s) 262, 421
BseXI GCAGC 1 cut(s) 202
Bsh1285I CGRYCG 1 cut(s) 136
BsiEI CGRYCG 1 cut(s) 136
BsiSI CCGG 1 cut(s) 329
Bsp13I TCCGGA 1 cut(s) 328
Bsp143I GATC 2 cut(s) 364, 432
BspEI TCCGGA 1 cut(s) 328
BspTI CTTAAG 1 cut(s) 308
BssECI CCNNGG 1 cut(s) 184
BssMI GATC 2 cut(s) 364, 432
Bst2UI CCWGG 1 cut(s) 254
Bst4CI ACNGT 2 cut(s) 134, 161
Bst6I CTCTTC 1 cut(s) 32
BstAFI CTTAAG 1 cut(s) 308
BstDEI CTNAG 1 cut(s) 242
BstDSI CCRYGG 1 cut(s) 184
BstF5I GGATG 2 cut(s) 262, 421
BstKTI GATC 2 cut(s) 367, 435
BstMBI GATC 2 cut(s) 364, 432
BstMCI CGRYCG 1 cut(s) 136
BstNI CCWGG 1 cut(s) 254
BstSCI CCNGG 1 cut(s) 252
BstV1I GCAGC 1 cut(s) 202
BstX2I RGATCY 1 cut(s) 432
BstXI CCANNNNNNTGG 1 cut(s) 185
BstYI RGATCY 1 cut(s) 432
BtgI CCRYGG 1 cut(s) 184
BtsCI GGATG 2 cut(s) 262, 421
CseI GACGC 1 cut(s) 158
CviJI RGCY 6 cut(s) 56, 76, 266, 299, 304, 325
CviKI_1 RGCY 6 cut(s) 56, 76, 266, 299, 304, 325
DdeI CTNAG 1 cut(s) 242
DpnI GATC 2 cut(s) 366, 434
DpnII GATC 2 cut(s) 364, 432
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
EcoRII CCWGG 1 cut(s) 252
EcoT22I ATGCAT 1 cut(s) 198
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 2 cut(s) 269, 428
Fsp4HI GCNGC 1 cut(s) 216
FspBI CTAG 1 cut(s) 300
GluI GCNGC 1 cut(s) 216
HapII CCGG 1 cut(s) 329
HgaI GACGC 1 cut(s) 158
HpaII CCGG 1 cut(s) 329
HphI GGTGA 4 cut(s) 233, 332, 374, 404
Hpy188I TCNGA 1 cut(s) 292
Hpy188III TCNNGA 2 cut(s) 329, 368
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 2 cut(s) 196, 344
HpyCH4III ACNGT 2 cut(s) 134, 161
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 2 cut(s) 153, 196
HpyF3I CTNAG 1 cut(s) 242
HpySE526I ACGT 1 cut(s) 144
Kpn2I TCCGGA 1 cut(s) 328
Kzo9I GATC 2 cut(s) 364, 432
LpnPI CCDG 5 cut(s) 19, 204, 239, 266, 342
Lsp1109I GCAGC 1 cut(s) 202
LweI GCATC 2 cut(s) 128, 183
MaeI CTAG 1 cut(s) 300
MaeII ACGT 1 cut(s) 144
MaeIII GTNAC 2 cut(s) 128, 221
MalI GATC 2 cut(s) 366, 434
MboI GATC 2 cut(s) 364, 432
MboII GAAGA 5 cut(s) 19, 49, 101, 107, 407
MflI RGATCY 1 cut(s) 432
MnlI CCTC 5 cut(s) 17, 35, 289, 406, 412
Mph1103I ATGCAT 1 cut(s) 198
MroI TCCGGA 1 cut(s) 328
MseI TTAA 3 cut(s) 165, 309, 345
MslI CAYNNNNRTG 1 cut(s) 183
MspCI CTTAAG 1 cut(s) 308
MspI CCGG 1 cut(s) 329
MspR9I CCNGG 1 cut(s) 254
MvaI CCWGG 1 cut(s) 254
NdeII GATC 2 cut(s) 364, 432
NmuCI GTSAC 1 cut(s) 221
NsiI ATGCAT 1 cut(s) 198
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PkrI GCNGC 1 cut(s) 217
Psp6I CCWGG 1 cut(s) 252
PspGI CCWGG 1 cut(s) 252
PsuI RGATCY 1 cut(s) 432
RseI CAYNNNNRTG 1 cut(s) 183
SaqAI TTAA 3 cut(s) 165, 309, 345
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 2 cut(s) 364, 432
ScrFI CCNGG 1 cut(s) 254
SetI ASST 7 cut(s) 58, 78, 147, 207, 268, 327, 355
SfaNI GCATC 2 cut(s) 128, 183
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 2 cut(s) 278, 308
SmoI CTYRAG 2 cut(s) 278, 308
SspMI CTAG 1 cut(s) 300
StyD4I CCNGG 1 cut(s) 252
TaaI ACNGT 2 cut(s) 134, 161
TaiI ACGT 1 cut(s) 147
TaqI TCGA 1 cut(s) 136
Tru1I TTAA 3 cut(s) 165, 309, 345
Tru9I TTAA 3 cut(s) 165, 309, 345
TseFI GTSAC 1 cut(s) 221
TseI GCWGC 1 cut(s) 215
Tsp45I GTSAC 1 cut(s) 221
TspGWI ACGGA 1 cut(s) 173
Vha464I CTTAAG 1 cut(s) 308
XspI CTAG 1 cut(s) 300
Zsp2I ATGCAT 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.