AT2G24645

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001490
Physical Location & Seq
Reverse (-)
10480515 .. 10482797
2283 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G24645.4

Sequence Viewer

Length: 1473 bp
ATGGCGAATCAACATTTCTTCAAGCCTCTTCTTCCTGGCTTCCACACCCACTTGAGAATACCTGTAGCCTTCTTCTTGAAGAATATAGAAGGAAGATATGAGCAGAAGACGGCGGAGCTGAGATCAGATGCGTCAAAGATAACTTGGGAAGTGAAGATAGATGGACAGAGACTCACTGACGGTTGGAAAGAGTTTGCTCTCTCACATGATCTTCGAATCGGTGACATTGTTGTTTTCAGACAAGAGAGAGATATGTCTTTCCACGTTACAATGTTGGGACCTAGTTGTTGTGAGATCCAATATGGTTCGTGTTCAGACGAGGAGAGAAACCTCGAGAAGAAGAAGAATCCAAATGGAGAAGCAAAGTCTTCTTCACTAGATCCTTCTTGTTTTTCGGCTAATGTGGCGCCTTCGAGTCTACGTTATGACTTAATGCTTTTTCCAATGGGTTTTGTGAGGGAAAATGGTGTAGTCGGATCTGGAAAGATTGTTCTGATGAATGAAAAGGGCAGATCATGGAATTTTAACTTGAGACAAAAGCCATCATGCGGAACAGTTTATGTTAGAGGAGGGTGGGTGAGTTTTTGTGATGCCAATGGACTTCAAGCTGGAGATATCTACACTTTCAAACTGATCAAAAGAGGAGGAACTCTTGTTCTACGTTTGTTACCCAAAGGGGCAGAGTCTTGTTCACTAGATCCCTCTTGTTTTGTGGCTAATGTTGCGCCTTCGACTCTACGCTATGACACACTGTATCTTCCAAAGCGTTTTATGAGGGAAAATGGTGTAGACAAAAGACGTGGAGAGATGATTCTGATGAATGAAAAGGGCAAATCATGGACTTTAGATTTGAAACTAAAGAAATCATGCGGAACTTCCCTCATCAGACGAGGATGGAGAAGTTTCTGTAGTGCCAATGGACTAAGAGCTGGAAGTATCATTACTTTCAAACTGATTAAGAAAAGTGGAACTCTTGTTCTATGTTTGCTCTCCAACGAGCCAGAAGAAGAAGTGTGCTCAGAAGCTAATGAAGTAGAGTCTCTTTCGACAGATCAAGAAAGCCATGAAGAGAGTAGCCACAACGAGAAAATCTCAAGAAGAAAGGAAAAGAAGGGTAGAATGATATGGAAAGCTTCGTCTTCACCATCCGAAAACCGATTTGTGACATTAAATCTTACACCTTATAACATCCTACGTTCTGCACTGCGTCTTCCGATACCCTTCACGAGGATGAATGGTATCAATGAAGAAACTAAAATGACTCTGTTGGATAAACATGGTATGAAGTGGTTAACGACTCTGCGGTTGGTGGACTACAAAAGAAAAAGACTGGGAATGGTAGGAGGCTGGAAAGGATTCATCCAAGCTAACGGTGTGAAGGCAAACGAATCCATCATGTTGGAACTGATTTGGGAAGAAGAAACAAGTTGCGTCCTTAAGTTCTGCTCCAAGGTGAAGCTAGCAATCAAATGA

Protein Analysis

490

Amino Acids

55.53

Weight (kDa)

9.39

Isoelectric Point (pI)

46.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 5 - 75 4.9e-08 B3 DNA binding domain
B3 PF02362 117 - 210 7.3e-24 B3 DNA binding domain
B3 PF02362 223 - 318 2.1e-25 B3 DNA binding domain
B3 PF02362 373 - 470 7.8e-20 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1185
AccB1I GGYRCC 1 cut(s) 406
AccI GTMKAC 2 cut(s) 418, 789
AciI CCGC 4 cut(s) 113, 549, 870, 1303
AclWI GGATC 4 cut(s) 289, 374, 484, 692
AcsI RAATTY 1 cut(s) 520
AcyI GRCGYC 1 cut(s) 407
AfiI CCNNNNNNNGG 2 cut(s) 548, 1227
AflII CTTAAG 1 cut(s) 1436
AgsI TTSAA 6 cut(s) 22, 79, 605, 628, 853, 949
AjiI CACGTC 1 cut(s) 800
AjnI CCWGG 1 cut(s) 34
AloI GAACNNNNNNTCC 6 cut(s) 474, 506, 639, 671, 960, 992
AluBI AGCT 7 cut(s) 118, 608, 929, 1025, 1133, 1367, 1459
AluI AGCT 7 cut(s) 118, 608, 929, 1025, 1133, 1367, 1459
Alw21I GWGCWC 1 cut(s) 1019
Alw26I GTCTC 3 cut(s) 163, 526, 1044
AlwI GGATC 4 cut(s) 289, 374, 484, 692
Ama87I CYCGRG 1 cut(s) 332
ApoI RAATTY 1 cut(s) 520
ArsI GACNNNNNNTTYG 2 cut(s) 832, 864
Asp700I GAANNNNTTC 1 cut(s) 1355
AspLEI GCGC 2 cut(s) 409, 727
AspS9I GGNCC 1 cut(s) 278
AsuHPI GGTGA 4 cut(s) 233, 589, 1134, 1465
AsuII TTCGAA 1 cut(s) 214
AsuNHI GCTAGC 1 cut(s) 1459
AvaI CYCGRG 1 cut(s) 332
AvaII GGWCC 1 cut(s) 278
BanI GGYRCC 1 cut(s) 406
BarI GAAGNNNNNNTAC 2 cut(s) 925, 957
BauI CACGAG 1 cut(s) 1225
BbsI GAAGAC 4 cut(s) 113, 360, 1131, 1202
Bbv12I GWGCWC 1 cut(s) 1019
BccI CCATC 5 cut(s) 155, 550, 888, 1153, 1400
BceAI ACGGC 1 cut(s) 126
BciT130I CCWGG 1 cut(s) 36
BclI TGATCA 1 cut(s) 633
BcoDI GTCTC 3 cut(s) 163, 526, 1044
BfaI CTAG 4 cut(s) 282, 377, 695, 1460
BfmI CTRYAG 2 cut(s) 63, 907
BfoI RGCGCY 1 cut(s) 410
BfrI CTTAAG 1 cut(s) 1436
Bme1390I CCNGG 1 cut(s) 36
Bme18I GGWCC 1 cut(s) 278
BmeT110I CYCGRG 1 cut(s) 332
BmgBI CACGTC 1 cut(s) 800
BmgT120I GGNCC 1 cut(s) 278
BmiI GGNNCC 2 cut(s) 279, 408
BmrFI CCNGG 1 cut(s) 36
BmrI ACTGGG 1 cut(s) 1340
BmsI GCATC 2 cut(s) 118, 580
BmtI GCTAGC 1 cut(s) 1463
BmuI ACTGGG 1 cut(s) 1340
BpiI GAAGAC 4 cut(s) 113, 360, 1131, 1202
BplI GAGNNNNNCTC 2 cut(s) 1076, 1108
BpmI CTGGAG 1 cut(s) 630
Bpu14I TTCGAA 1 cut(s) 214
BpuEI CTTGAG 3 cut(s) 73, 550, 1078
BsaHI GRCGYC 1 cut(s) 407
BsaJI CCNNGG 1 cut(s) 1449
BsaXI ACNNNNNCTCC 2 cut(s) 561, 591
Bsc4I CCNNNNNNNGG 2 cut(s) 548, 1227
Bse1I ACTGG 1 cut(s) 1335
BseBI CCWGG 1 cut(s) 36
BseDI CCNNGG 1 cut(s) 1449
BseGI GGATG 5 cut(s) 899, 1145, 1188, 1236, 1359
BseLI CCNNNNNNNGG 2 cut(s) 548, 1227
BseMII CTCAG 2 cut(s) 110, 1032
BseNI ACTGG 1 cut(s) 1335
BseRI GAGGAG 3 cut(s) 335, 582, 657
BsgI GTGCAG 1 cut(s) 1185
BshNI GGYRCC 1 cut(s) 406
BsiHKAI GWGCWC 1 cut(s) 1019
BsiHKCI CYCGRG 1 cut(s) 332
BslFI GGGAC 1 cut(s) 291
BslI CCNNNNNNNGG 2 cut(s) 548, 1227
BsmAI GTCTC 3 cut(s) 163, 526, 1044
BsmFI GGGAC 1 cut(s) 291
BsoBI CYCGRG 1 cut(s) 332
Bsp119I TTCGAA 1 cut(s) 214
Bsp1286I GDGCHC 1 cut(s) 1019
Bsp143I GATC 9 cut(s) 122, 208, 294, 379, 476, 512, 633, 697, 1051
BspACI CCGC 4 cut(s) 113, 549, 870, 1303
BspCNI CTCAG 2 cut(s) 111, 1031
BspLI GGNNCC 2 cut(s) 279, 408
BspOI GCTAGC 1 cut(s) 1463
BspPI GGATC 4 cut(s) 289, 374, 484, 692
BspT104I TTCGAA 1 cut(s) 214
BspT107I GGYRCC 1 cut(s) 406
BspTI CTTAAG 1 cut(s) 1436
BsrI ACTGG 1 cut(s) 1335
BssECI CCNNGG 1 cut(s) 1449
BssMI GATC 9 cut(s) 122, 208, 294, 379, 476, 512, 633, 697, 1051
BssNI GRCGYC 1 cut(s) 407
BssSI CACGAG 1 cut(s) 1225
BssT1I CCWWGG 1 cut(s) 1449
Bst2BI CACGAG 1 cut(s) 1225
Bst2UI CCWGG 1 cut(s) 36
Bst4CI ACNGT 4 cut(s) 182, 556, 753, 1373
Bst6I CTCTTC 2 cut(s) 33, 1062
BstACI GRCGYC 1 cut(s) 407
BstAFI CTTAAG 1 cut(s) 1436
BstBI TTCGAA 1 cut(s) 214
BstC8I GCNNGC 1 cut(s) 1461
BstDEI CTNAG 3 cut(s) 119, 923, 1018
BstENI CCTNNNNNAGG 1 cut(s) 1225
BstF5I GGATG 5 cut(s) 899, 1145, 1188, 1236, 1359
BstH2I RGCGCY 1 cut(s) 410
BstHHI GCGC 2 cut(s) 409, 727
BstKTI GATC 9 cut(s) 125, 211, 297, 382, 479, 515, 636, 700, 1054
BstMAI GTCTC 3 cut(s) 163, 526, 1044
BstMBI GATC 9 cut(s) 122, 208, 294, 379, 476, 512, 633, 697, 1051
BstMWI GCNNNNNNNGC 2 cut(s) 404, 722
BstNI CCWGG 1 cut(s) 36
BstSCI CCNGG 1 cut(s) 34
BstSFI CTRYAG 2 cut(s) 63, 907
BstV2I GAAGAC 4 cut(s) 113, 360, 1131, 1202
BstX2I RGATCY 4 cut(s) 294, 379, 476, 697
BstXI CCANNNNNNTGG 1 cut(s) 1399
BstYI RGATCY 4 cut(s) 294, 379, 476, 697
BtrI CACGTC 1 cut(s) 800
BtsCI GGATG 5 cut(s) 899, 1145, 1188, 1236, 1359
BtsI GCAGTG 1 cut(s) 1202
BtsIMutI CAGTG 3 cut(s) 174, 749, 1202
Cac8I GCNNGC 1 cut(s) 1461
CfoI GCGC 2 cut(s) 409, 727
Cfr13I GGNCC 1 cut(s) 278
CseI GACGC 3 cut(s) 120, 1196, 1420
CspCI CAANNNNNGTGG 2 cut(s) 781, 816
CviAII CATG 8 cut(s) 206, 516, 546, 837, 867, 1064, 1277, 1396
DdeI CTNAG 3 cut(s) 119, 923, 1018
DinI GGCGCC 1 cut(s) 408
DpnI GATC 9 cut(s) 124, 210, 296, 381, 478, 514, 635, 699, 1053
DpnII GATC 9 cut(s) 122, 208, 294, 379, 476, 512, 633, 697, 1051
Eam1104I CTCTTC 2 cut(s) 33, 1062
EarI CTCTTC 2 cut(s) 33, 1062
EciI GGCGGA 1 cut(s) 128
Eco130I CCWWGG 1 cut(s) 1449
Eco32I GATATC 1 cut(s) 616
Eco47I GGWCC 1 cut(s) 278
Eco88I CYCGRG 1 cut(s) 332
EcoNI CCTNNNNNAGG 1 cut(s) 1225
EcoO109I RGGNCCY 1 cut(s) 278
EcoRII CCWGG 1 cut(s) 34
EcoRV GATATC 1 cut(s) 616
EcoT14I CCWWGG 1 cut(s) 1449
EgeI GGCGCC 1 cut(s) 408
EheI GGCGCC 1 cut(s) 408
ErhI CCWWGG 1 cut(s) 1449
FaeI CATG 8 cut(s) 209, 519, 549, 840, 870, 1067, 1280, 1399
FaqI GGGAC 1 cut(s) 291
FatI CATG 8 cut(s) 205, 515, 545, 836, 866, 1063, 1276, 1395
FbaI TGATCA 1 cut(s) 633
FblI GTMKAC 2 cut(s) 418, 789
FokI GGATG 5 cut(s) 906, 1132, 1175, 1243, 1346
FspBI CTAG 4 cut(s) 282, 377, 695, 1460
GlaI GCGC 2 cut(s) 408, 726
GsuI CTGGAG 1 cut(s) 630
HaeII RGCGCY 1 cut(s) 410
HgaI GACGC 3 cut(s) 120, 1196, 1420
HhaI GCGC 2 cut(s) 409, 727
Hin1I GRCGYC 1 cut(s) 407
Hin1II CATG 8 cut(s) 209, 519, 549, 840, 870, 1067, 1280, 1399
Hin6I GCGC 2 cut(s) 407, 725
HinP1I GCGC 2 cut(s) 407, 725
HincII GTYRAC 1 cut(s) 1293
HindII GTYRAC 1 cut(s) 1293
HindIII AAGCTT 1 cut(s) 1131
HpaI GTTAAC 1 cut(s) 1293
HphI GGTGA 4 cut(s) 233, 589, 1134, 1465
Hpy166II GTNNAC 5 cut(s) 419, 692, 790, 1293, 1312
Hpy188III TCNNGA 6 cut(s) 76, 334, 480, 1055, 1095, 1225
Hpy8I GTNNAC 5 cut(s) 419, 692, 790, 1293, 1312
HpyAV CCTTC 8 cut(s) 79, 83, 393, 420, 738, 1105, 1231, 1372
HpyCH4III ACNGT 4 cut(s) 182, 556, 753, 1373
HpyCH4IV ACGT 5 cut(s) 264, 421, 661, 799, 1195
HpyCH4V TGCA 1 cut(s) 1202
HpyF10VI GCNNNNNNNGC 2 cut(s) 404, 722
HpyF3I CTNAG 3 cut(s) 119, 923, 1018
HpySE526I ACGT 5 cut(s) 264, 421, 661, 799, 1195
Hsp92I GRCGYC 1 cut(s) 407
Hsp92II CATG 8 cut(s) 209, 519, 549, 840, 870, 1067, 1280, 1399
HspAI GCGC 2 cut(s) 407, 725
KasI GGCGCC 1 cut(s) 406
Ksp22I TGATCA 1 cut(s) 633
KspAI GTTAAC 1 cut(s) 1293
Kzo9I GATC 9 cut(s) 122, 208, 294, 379, 476, 512, 633, 697, 1051
LmnI GCTCC 2 cut(s) 115, 1451
LpnPI CCDG 9 cut(s) 21, 48, 75, 465, 594, 915, 1014, 1316, 1333
LweI GCATC 2 cut(s) 118, 580
MaeI CTAG 4 cut(s) 282, 377, 695, 1460
MaeII ACGT 5 cut(s) 264, 421, 661, 799, 1195
MaeIII GTNAC 4 cut(s) 221, 265, 666, 1162
MalI GATC 9 cut(s) 124, 210, 296, 381, 478, 514, 635, 699, 1053
MboI GATC 9 cut(s) 122, 208, 294, 379, 476, 512, 633, 697, 1051
MflI RGATCY 4 cut(s) 294, 379, 476, 697
MhlI GDGCHC 1 cut(s) 1019
MluCI AATT 1 cut(s) 520
Mly113I GGCGCC 1 cut(s) 407
MlyI GAGTC 7 cut(s) 165, 424, 692, 727, 1046, 1255, 1291
MmeI TCCRAC 5 cut(s) 164, 454, 1017, 1248, 1380
MroXI GAANNNNTTC 1 cut(s) 1355
MseI TTAA 6 cut(s) 431, 525, 957, 1169, 1292, 1437
MslI CAYNNNNRTG 1 cut(s) 1229
MspCI CTTAAG 1 cut(s) 1436
MspR9I CCNGG 1 cut(s) 36
MvaI CCWGG 1 cut(s) 36
MwoI GCNNNNNNNGC 2 cut(s) 404, 722
NarI GGCGCC 1 cut(s) 407
NdeII GATC 9 cut(s) 122, 208, 294, 379, 476, 512, 633, 697, 1051
NheI GCTAGC 1 cut(s) 1459
NlaIII CATG 8 cut(s) 209, 519, 549, 840, 870, 1067, 1280, 1399
NlaIV GGNNCC 2 cut(s) 279, 408
NmuCI GTSAC 2 cut(s) 221, 1162
NspV TTCGAA 1 cut(s) 214
PaeR7I CTCGAG 1 cut(s) 332
PdmI GAANNNNTTC 1 cut(s) 1355
PfeI GAWTC 6 cut(s) 7, 216, 346, 811, 1356, 1388
PleI GAGTC 7 cut(s) 165, 423, 691, 727, 1045, 1255, 1291
PluTI GGCGCC 1 cut(s) 410
PpsI GAGTC 7 cut(s) 165, 423, 691, 727, 1045, 1255, 1291
PpuMI RGGWCCY 1 cut(s) 278
PsiI TTATAA 1 cut(s) 1185
Psp5II RGGWCCY 1 cut(s) 278
Psp6I CCWGG 1 cut(s) 34
PspGI CCWGG 1 cut(s) 34
PspN4I GGNNCC 2 cut(s) 279, 408
PspPI GGNCC 1 cut(s) 278
PspPPI RGGWCCY 1 cut(s) 278
PsuI RGATCY 4 cut(s) 294, 379, 476, 697
RseI CAYNNNNRTG 1 cut(s) 1229
SaqAI TTAA 6 cut(s) 431, 525, 957, 1169, 1292, 1437
Sau3AI GATC 9 cut(s) 122, 208, 294, 379, 476, 512, 633, 697, 1051
Sau96I GGNCC 1 cut(s) 278
SchI GAGTC 7 cut(s) 165, 424, 692, 727, 1046, 1255, 1291
ScrFI CCNGG 1 cut(s) 36
SduI GDGCHC 1 cut(s) 1019
SfaNI GCATC 2 cut(s) 118, 580
SfcI CTRYAG 2 cut(s) 63, 907
SfoI GGCGCC 1 cut(s) 408
Sfr274I CTCGAG 1 cut(s) 332
SfuI TTCGAA 1 cut(s) 214
SinI GGWCC 1 cut(s) 278
SlaI CTCGAG 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 1229
SmlI CTYRAG 5 cut(s) 52, 332, 529, 1093, 1436
SmoI CTYRAG 5 cut(s) 52, 332, 529, 1093, 1436
Sse9I AATT 1 cut(s) 520
SsiI CCGC 4 cut(s) 113, 549, 870, 1303
SspDI GGCGCC 1 cut(s) 406
SspMI CTAG 4 cut(s) 282, 377, 695, 1460
StyD4I CCNGG 1 cut(s) 34
StyI CCWWGG 1 cut(s) 1449
TaaI ACNGT 4 cut(s) 182, 556, 753, 1373
TaiI ACGT 5 cut(s) 267, 424, 664, 802, 1198
TaqI TCGA 5 cut(s) 214, 333, 413, 731, 1046
TasI AATT 1 cut(s) 520
TfiI GAWTC 6 cut(s) 7, 216, 346, 811, 1356, 1388
Tru1I TTAA 6 cut(s) 431, 525, 957, 1169, 1292, 1437
Tru9I TTAA 6 cut(s) 431, 525, 957, 1169, 1292, 1437
TscAI CASTG 3 cut(s) 181, 756, 1209
TseFI GTSAC 2 cut(s) 221, 1162
Tsp45I GTSAC 2 cut(s) 221, 1162
TspRI CASTG 3 cut(s) 181, 756, 1209
Vha464I CTTAAG 1 cut(s) 1436
VpaK11BI GGWCC 1 cut(s) 278
XagI CCTNNNNNAGG 1 cut(s) 1225
XapI RAATTY 1 cut(s) 520
XhoI CTCGAG 1 cut(s) 332
XmiI GTMKAC 2 cut(s) 418, 789
XmnI GAANNNNTTC 1 cut(s) 1355
XspI CTAG 4 cut(s) 282, 377, 695, 1460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.