AT2G24690

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001490
Physical Location & Seq
Reverse (-)
10498972 .. 10503188
4217 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G24690.2

Sequence Viewer

Length: 2334 bp
ATGGCGAATTCACTCATTCTGGGCTCACCGATTAATCCAGAAGGAGAAATGATGATCCAATTCTCAAATAATCATGACAACAACATCGAGTTTCAAAGAAGGAAGAAAATGAAGAAGAACAATCCTGAAACAGAAACTGATTCTTCATCAGATCTCTCTTGTTTTGTTGCCCAAACAGGTGCACTGCTTCATCTTCCACAAGGTTTCACTAGCTCAAATGGTAGTAATAGGGAATGTAGAAAGAGTCCTAGACCGATTATATCATCATATTCGTCAAGCGAGAAACAGTTTGTAACGTTTACACTTGCACCTGTTGATGGCAGACATTGTAGACTGCGTCTTCCGATGCAATTCACGAGGGAGAATGGGATTAACAAGCCTGGGAAGATATATTTGGTGGGTAAAGATGGTTCTAAGTGGCTTGCAAATCTTCTTTTGGAAAACAACAGAGGAAGAATGACTTTAGGAGATGGTTGGAAAAGTTTTGTTAAAGCAAACGGTTTAAAGACTGGTGATACCTATACGTTCAAGTTACTGTGGGAAGACACAACTCCTGTGCTCAGTTTGTGTTTTGAAGAGTACAACACTGACACAAGAGTAGGAGAAGAGTCTTCGAAAGAGTCTCTTCCCGCAGAGCCTAGTAGCCAAGAAAAGATCGTTAAAGATGACAACAACAAAGATGAAAGCAGCACATGGAAGAGAGAGGGTAATCATCTGAGATGCAAAGATTCAACTTCACCAAGCCAGAACTGCACCTTGACAGTAACAATCACGCCTGATAGTCTCGAACATGGTAGACTACGGCTTCCGTTGCAATTCATGACGGAGAATAGCATGAACAAGCCCGGGGAGATAACTCTTTTAGGTACAGATGGTGCAAAGTGGATGGCAAGTCTTCTACTAGAAAAAAAGGGAAGAATGAGTTTGGGAAAGGGTTGGAAAGATTTTGCTAAAGCAAATGGCTTAAAGACGGGTGATTCCATTACATTGGAGCCAATATGGGAAGACAGGACTCCTGTGCTCAGTATAAAGTCTAGCAGTGGTAAAGGGCAAAGTGAGTTTTCCAAGGAATCTCTTTCCATAAAACCTAGTAGTGGAAACATGACTAAGAAAGTAGAGAACAACAGAGAAGCAAGCAGAAAGTATCCTCCTAGAAGTAGAGAATCATCTTCAGCAATCCAAAACCAATTCATGGCATTAACACCTCTACGTGATATAGTTAGCCAGGTTGCTCATGATCTTAGCATCGGTGAGGTCATCAATTTCAGACATAAAGGAGATAATATGTTGCGGGTCTCAGATTTGGGATCCAATTGTTGTGGGGTTCAAGATTTACTGGCTCCAAGCAGTAACTATGATCATGACAACATCAGTAACATTTCGATGAAAATAAATCCGCATATAAGAAAAGAAGCTGTAACATTTTCGTCATATGATGGTTATGCTCATGACAACTTTGAGCCTCCAAGTAAGAAGAAAGTGAAGAAGAACAATCCAGAAATGGAAGCAGATTATTTATCAGACCACTCTTGTTTTGTATCCCATGTCACAACTTCAAGTCTACACACAAATGCGTTGAGCCTAGAAGTGAAAGAGTCTCTTCCCATAGAGCCTAGCATTAAACCAAATATCAGTGAAGATGACAACAACAAAGAGGAAGACATCATAGAGGAGAACAGCTCATTTGAAAGAGAGAAGAATCATTGGATATGTATAGATTCAATTTCATCGAGCCAAAACCGATTTCTGACATTAACAATCACACCTGACAGTCTCAAACATGGTAGACTGCGTCTTCCACTGCAATTCATGATAGAGAATAGCATGAATAAGCCCGGAGAGATAACTGTGTTAGGTAAAGATGGTGCGAAGTGGCTGGTAAGTCTTCTACTGGAAAGAAGAGGAAGAATGAGTTTGGATGCTAGCAGTGGACAAGAGACCAGGGAAGCAGAGAAAAACAGAGAAGAGAGTAGTAGCTTATGGGAGTTGGAGAAAAGAACCTATTGTCCAAGAAAACGAGATTCATCGTCAGATGTCAAAAACCGATTCCTTACACTAACACTTGCACCTGAAGATGTCAAGGATGGTAATTTGCATCTTCCTTGTCAATTCATGAGAATTAATGGCATCAACAAGCCTGGACAGATAACTTTGTTGGGTAGAGGCGGTATGAAGTGGTTTGCATATTTACTGTCAGGAGATGGAACTGTCGTTGTGGGAAATGGTTGGAAAGGTTTTTGTGAGGCGAATGGTGTGATGTTAGGAGAGACTTTTGTTTTGGAATTCATTCCCAAAGATGATACAAATCATGTATTTAAGTTCTACACCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

777

Amino Acids

87.16

Weight (kDa)

7.56

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 97 - 191 2.4e-25 B3 DNA binding domain
B3 PF02362 253 - 346 2.8e-23 B3 DNA binding domain
B3 PF02362 582 - 675 2.3e-23 B3 DNA binding domain
B3 PF02362 738 - 830 2.1e-14 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1192
AccI GTMKAC 4 cut(s) 331, 796, 1561, 1786
AciI CCGC 4 cut(s) 630, 1291, 1397, 2166
AclI AACGTT 1 cut(s) 296
AclWI GGATC 3 cut(s) 49, 1302, 1315
AcsI RAATTY 2 cut(s) 7, 2282
AcuI CTGAAG 2 cut(s) 1155, 2091
AfaI GTAC 2 cut(s) 581, 868
AfiI CCNNNNNNNGG 3 cut(s) 317, 1094, 1192
AgsI TTSAA 8 cut(s) 95, 529, 575, 732, 1328, 1557, 1688, 1722
AjnI CCWGG 4 cut(s) 379, 1224, 1940, 2137
AjuI GAANNNNNNNTTGG 4 cut(s) 377, 409, 1728, 1760
AloI GAACNNNNNNTCC 2 cut(s) 1990, 2022
AluBI AGCT 4 cut(s) 213, 1415, 1680, 1977
AluI AGCT 4 cut(s) 213, 1415, 1680, 1977
Alw21I GWGCWC 3 cut(s) 184, 561, 1023
Alw26I GTCTC 7 cut(s) 627, 788, 1300, 1602, 1778, 1931, 2261
Alw44I GTGCAC 1 cut(s) 180
AlwI GGATC 3 cut(s) 49, 1302, 1315
AlwNI CAGNNNCTG 1 cut(s) 137
Ama87I CYCGRG 1 cut(s) 845
ApaLI GTGCAC 1 cut(s) 180
ApeKI GCWGC 1 cut(s) 687
ApoI RAATTY 2 cut(s) 7, 2282
ArsI GACNNNNNNTTYG 2 cut(s) 2260, 2292
AseI ATTAAT 2 cut(s) 33, 2121
Asp700I GAANNNNTTC 1 cut(s) 2286
AsuC2I CCSGG 3 cut(s) 846, 847, 1836
AsuHPI GGTGA 5 cut(s) 18, 524, 729, 986, 1262
AsuII TTCGAA 1 cut(s) 614
AsuNHI GCTAGC 1 cut(s) 1922
AvaI CYCGRG 1 cut(s) 845
BaeGI GKGCMC 1 cut(s) 184
BamHI GGATCC 1 cut(s) 1307
BanII GRGCYC 1 cut(s) 26
BauI CACGAG 1 cut(s) 355
BbsI GAAGAC 8 cut(s) 332, 549, 603, 887, 1011, 1665, 1787, 1877
Bbv12I GWGCWC 3 cut(s) 184, 561, 1023
BbvI GCAGC 1 cut(s) 699
BccI CCATC 9 cut(s) 311, 401, 464, 866, 880, 1430, 1856, 2078, 2195
BceAI ACGGC 1 cut(s) 818
BciT130I CCWGG 4 cut(s) 381, 1226, 1942, 2139
BciVI GTATCC 2 cut(s) 1155, 1549
BclI TGATCA 1 cut(s) 1357
BcnI CCSGG 3 cut(s) 846, 847, 1836
BcoDI GTCTC 7 cut(s) 627, 788, 1300, 1602, 1778, 1931, 2261
BfuI GTATCC 2 cut(s) 1155, 1549
BglII AGATCT 1 cut(s) 151
BisI GCNGC 1 cut(s) 688
BlsI GCNGC 1 cut(s) 689
Bme1390I CCNGG 7 cut(s) 381, 846, 847, 1226, 1836, 1942, 2139
BmeT110I CYCGRG 1 cut(s) 845
BmiI GGNNCC 3 cut(s) 993, 1309, 1341
BmrFI CCNGG 7 cut(s) 381, 846, 847, 1226, 1836, 1942, 2139
BmsI GCATC 6 cut(s) 336, 710, 1254, 1909, 2104, 2136
BmtI GCTAGC 1 cut(s) 1926
BpiI GAAGAC 8 cut(s) 332, 549, 603, 887, 1011, 1665, 1787, 1877
BplI GAGNNNNNCTC 2 cut(s) 1664, 1696
Bpu14I TTCGAA 1 cut(s) 614
BpuMI CCSGG 3 cut(s) 846, 847, 1836
BsaAI YACGTR 1 cut(s) 1211
BsaBI GATNNNNATC 1 cut(s) 2304
BsaI GGTCTC 2 cut(s) 1300, 1931
BsaJI CCNNGG 5 cut(s) 380, 845, 846, 1065, 1941
Bsc4I CCNNNNNNNGG 3 cut(s) 317, 1094, 1192
Bse1I ACTGG 3 cut(s) 514, 1341, 1896
Bse8I GATNNNNATC 1 cut(s) 2304
BseBI CCWGG 4 cut(s) 381, 1226, 1942, 2139
BseDI CCNNGG 5 cut(s) 380, 845, 846, 1065, 1941
BseGI GGATG 3 cut(s) 891, 1924, 2089
BseJI GATNNNNATC 1 cut(s) 2304
BseLI CCNNNNNNNGG 3 cut(s) 317, 1094, 1192
BseMII CTCAG 4 cut(s) 574, 707, 1036, 1311
BseNI ACTGG 3 cut(s) 514, 1341, 1896
BseRI GAGGAG 1 cut(s) 1685
BseSI GKGCMC 1 cut(s) 184
BseXI GCAGC 1 cut(s) 699
BsgI GTGCAG 1 cut(s) 736
BsiHKAI GWGCWC 3 cut(s) 184, 561, 1023
BsiHKCI CYCGRG 1 cut(s) 845
BsiSI CCGG 2 cut(s) 846, 1836
BslI CCNNNNNNNGG 3 cut(s) 317, 1094, 1192
BsmAI GTCTC 7 cut(s) 627, 788, 1300, 1602, 1778, 1931, 2261
Bso31I GGTCTC 2 cut(s) 1300, 1931
BsoBI CYCGRG 1 cut(s) 845
Bsp119I TTCGAA 1 cut(s) 614
Bsp1286I GDGCHC 4 cut(s) 26, 184, 561, 1023
Bsp143I GATC 6 cut(s) 54, 151, 654, 1237, 1307, 1357
BspACI CCGC 4 cut(s) 630, 1291, 1397, 2166
BspCNI CTCAG 4 cut(s) 573, 708, 1035, 1310
BspHI TCATGA 7 cut(s) 73, 819, 1234, 1360, 1447, 1809, 2112
BspLI GGNNCC 3 cut(s) 993, 1309, 1341
BspOI GCTAGC 1 cut(s) 1926
BspPI GGATC 3 cut(s) 49, 1302, 1315
BspT104I TTCGAA 1 cut(s) 614
BspTNI GGTCTC 2 cut(s) 1300, 1931
BsrI ACTGG 3 cut(s) 514, 1341, 1896
BssECI CCNNGG 5 cut(s) 380, 845, 846, 1065, 1941
BssMI GATC 6 cut(s) 54, 151, 654, 1237, 1307, 1357
BssSI CACGAG 1 cut(s) 355
BssT1I CCWWGG 1 cut(s) 1065
Bst2BI CACGAG 1 cut(s) 355
Bst2UI CCWGG 4 cut(s) 381, 1226, 1942, 2139
Bst4CI ACNGT 8 cut(s) 288, 500, 537, 763, 1772, 1849, 2193, 2209
Bst6I CTCTTC 7 cut(s) 570, 600, 630, 692, 1605, 1894, 1959
BstBAI YACGTR 1 cut(s) 1211
BstBI TTCGAA 1 cut(s) 614
BstC8I GCNNGC 3 cut(s) 423, 1135, 1924
BstDEI CTNAG 7 cut(s) 414, 560, 716, 1022, 1107, 1241, 1297
BstF5I GGATG 3 cut(s) 891, 1924, 2089
BstKTI GATC 6 cut(s) 57, 154, 657, 1240, 1310, 1360
BstMAI GTCTC 7 cut(s) 627, 788, 1300, 1602, 1778, 1931, 2261
BstMBI GATC 6 cut(s) 54, 151, 654, 1237, 1307, 1357
BstMWI GCNNNNNNNGC 2 cut(s) 750, 811
BstNI CCWGG 4 cut(s) 381, 1226, 1942, 2139
BstSCI CCNGG 7 cut(s) 379, 844, 845, 1224, 1834, 1940, 2137
BstSLI GKGCMC 1 cut(s) 184
BstV1I GCAGC 1 cut(s) 699
BstV2I GAAGAC 8 cut(s) 332, 549, 603, 887, 1011, 1665, 1787, 1877
BstX2I RGATCY 2 cut(s) 151, 1307
BstXI CCANNNNNNTGG 1 cut(s) 988
BstYI RGATCY 2 cut(s) 151, 1307
BsuI GTATCC 2 cut(s) 1155, 1549
BtsCI GGATG 3 cut(s) 891, 1924, 2089
BtsI GCAGTG 4 cut(s) 182, 1045, 1799, 1933
BtsIMutI CAGTG 6 cut(s) 182, 585, 1045, 1639, 1799, 1933
Cac8I GCNNGC 3 cut(s) 423, 1135, 1924
CaiI CAGNNNCTG 1 cut(s) 137
CciI TCATGA 7 cut(s) 73, 819, 1234, 1360, 1447, 1809, 2112
Cfr9I CCCGGG 1 cut(s) 845
CseI GACGC 2 cut(s) 326, 1781
Csp6I GTAC 2 cut(s) 580, 867
CspCI CAANNNNNGTGG 2 cut(s) 1300, 1335
CviQI GTAC 2 cut(s) 580, 867
DdeI CTNAG 7 cut(s) 414, 560, 716, 1022, 1107, 1241, 1297
DpnI GATC 6 cut(s) 56, 153, 656, 1239, 1309, 1359
DpnII GATC 6 cut(s) 54, 151, 654, 1237, 1307, 1357
DraI TTTAAA 1 cut(s) 504
Eam1104I CTCTTC 7 cut(s) 570, 600, 630, 692, 1605, 1894, 1959
EarI CTCTTC 7 cut(s) 570, 600, 630, 692, 1605, 1894, 1959
Eco130I CCWWGG 1 cut(s) 1065
Eco24I GRGCYC 1 cut(s) 26
Eco31I GGTCTC 2 cut(s) 1300, 1931
Eco57I CTGAAG 2 cut(s) 1155, 2091
Eco88I CYCGRG 1 cut(s) 845
EcoRI GAATTC 2 cut(s) 7, 2282
EcoRII CCWGG 4 cut(s) 379, 1224, 1940, 2137
EcoT14I CCWWGG 1 cut(s) 1065
EcoT38I GRGCYC 1 cut(s) 26
ErhI CCWWGG 1 cut(s) 1065
FalI AAGNNNNNCTT 6 cut(s) 445, 477, 609, 641, 1584, 1616
FauI CCCGC 2 cut(s) 637, 1284
FauNDI CATATG 1 cut(s) 1432
FbaI TGATCA 1 cut(s) 1357
FblI GTMKAC 4 cut(s) 331, 796, 1561, 1786
Fnu4HI GCNGC 1 cut(s) 688
FokI GGATG 3 cut(s) 898, 1931, 2096
FriOI GRGCYC 1 cut(s) 26
Fsp4HI GCNGC 1 cut(s) 688
GluI GCNGC 1 cut(s) 688
HapII CCGG 2 cut(s) 846, 1836
HgaI GACGC 2 cut(s) 326, 1781
HpaII CCGG 2 cut(s) 846, 1836
HphI GGTGA 5 cut(s) 18, 524, 729, 986, 1262
Hpy166II GTNNAC 7 cut(s) 182, 300, 332, 797, 1562, 1787, 1931
Hpy188I TCNGA 8 cut(s) 151, 345, 717, 1268, 1300, 1522, 1749, 2032
Hpy8I GTNNAC 7 cut(s) 182, 300, 332, 797, 1562, 1787, 1931
HpyAV CCTTC 2 cut(s) 35, 93
HpyCH4III ACNGT 8 cut(s) 288, 500, 537, 763, 1772, 1849, 2193, 2209
HpyCH4IV ACGT 3 cut(s) 296, 524, 1210
HpyF10VI GCNNNNNNNGC 2 cut(s) 750, 811
HpyF3I CTNAG 7 cut(s) 414, 560, 716, 1022, 1107, 1241, 1297
HpySE526I ACGT 3 cut(s) 296, 524, 1210
Ksp22I TGATCA 1 cut(s) 1357
Kzo9I GATC 6 cut(s) 54, 151, 654, 1237, 1307, 1357
LmnI GCTCC 2 cut(s) 991, 1345
Lsp1109I GCAGC 1 cut(s) 699
LweI GCATC 6 cut(s) 336, 710, 1254, 1909, 2104, 2136
MaeII ACGT 3 cut(s) 296, 524, 1210
MaeIII GTNAC 7 cut(s) 292, 531, 763, 1349, 1373, 1417, 1546
MalI GATC 6 cut(s) 56, 153, 656, 1239, 1309, 1359
MboI GATC 6 cut(s) 54, 151, 654, 1237, 1307, 1357
MfeI CAATTG 1 cut(s) 1312
MflI RGATCY 2 cut(s) 151, 1307
MhlI GDGCHC 4 cut(s) 26, 184, 561, 1023
MlyI GAGTC 5 cut(s) 253, 617, 629, 1006, 1604
MmeI TCCRAC 4 cut(s) 455, 917, 1968, 2207
MroXI GAANNNNTTC 1 cut(s) 2286
MslI CAYNNNNRTG 2 cut(s) 1382, 1569
MspI CCGG 2 cut(s) 846, 1836
MspR9I CCNGG 7 cut(s) 381, 846, 847, 1226, 1836, 1942, 2139
MunI CAATTG 1 cut(s) 1312
MvaI CCWGG 4 cut(s) 381, 1226, 1942, 2139
MwoI GCNNNNNNNGC 2 cut(s) 750, 811
NciI CCSGG 3 cut(s) 846, 847, 1836
NdeI CATATG 1 cut(s) 1432
NdeII GATC 6 cut(s) 54, 151, 654, 1237, 1307, 1357
NheI GCTAGC 1 cut(s) 1922
NlaIV GGNNCC 3 cut(s) 993, 1309, 1341
NmuCI GTSAC 1 cut(s) 1546
NspV TTCGAA 1 cut(s) 614
PagI TCATGA 7 cut(s) 73, 819, 1234, 1360, 1447, 1809, 2112
PdmI GAANNNNTTC 1 cut(s) 2286
PfeI GAWTC 9 cut(s) 140, 728, 977, 1070, 1163, 1699, 1718, 2021, 2046
PflFI GACNNNGTC 2 cut(s) 336, 1791
PflMI CCANNNNNTGG 1 cut(s) 1192
PkrI GCNGC 1 cut(s) 689
PleI GAGTC 5 cut(s) 252, 616, 628, 1006, 1603
PpsI GAGTC 5 cut(s) 252, 616, 628, 1006, 1603
Ppu21I YACGTR 1 cut(s) 1211
PshBI ATTAAT 2 cut(s) 33, 2121
Psp1406I AACGTT 1 cut(s) 296
Psp6I CCWGG 4 cut(s) 379, 1224, 1940, 2137
PspGI CCWGG 4 cut(s) 379, 1224, 1940, 2137
PspN4I GGNNCC 3 cut(s) 993, 1309, 1341
PsrI GAACNNNNNNTAC 2 cut(s) 394, 426
PstNI CAGNNNCTG 1 cut(s) 137
PsuI RGATCY 2 cut(s) 151, 1307
PsyI GACNNNGTC 2 cut(s) 336, 1791
RsaI GTAC 2 cut(s) 581, 868
RsaNI GTAC 2 cut(s) 580, 867
RseI CAYNNNNRTG 2 cut(s) 1382, 1569
SatI GCNGC 1 cut(s) 688
Sau3AI GATC 6 cut(s) 54, 151, 654, 1237, 1307, 1357
SchI GAGTC 5 cut(s) 253, 617, 629, 1006, 1604
ScrFI CCNGG 7 cut(s) 381, 846, 847, 1226, 1836, 1942, 2139
SduI GDGCHC 4 cut(s) 26, 184, 561, 1023
SfaNI GCATC 6 cut(s) 336, 710, 1254, 1909, 2104, 2136
SfuI TTCGAA 1 cut(s) 614
SmaI CCCGGG 1 cut(s) 847
SmiMI CAYNNNNRTG 2 cut(s) 1382, 1569
SsiI CCGC 4 cut(s) 630, 1291, 1397, 2166
StyD4I CCNGG 7 cut(s) 379, 844, 845, 1224, 1834, 1940, 2137
StyI CCWWGG 1 cut(s) 1065
TaaI ACNGT 8 cut(s) 288, 500, 537, 763, 1772, 1849, 2193, 2209
TaiI ACGT 3 cut(s) 299, 527, 1213
TaqI TCGA 5 cut(s) 87, 614, 786, 1382, 1730
TaqII GACCGA 1 cut(s) 268
TatI WGTACW 1 cut(s) 579
TfiI GAWTC 9 cut(s) 140, 728, 977, 1070, 1163, 1699, 1718, 2021, 2046
TscAI CASTG 6 cut(s) 189, 592, 1045, 1639, 1806, 1933
TseFI GTSAC 1 cut(s) 1546
TseI GCWGC 1 cut(s) 687
Tsp45I GTSAC 1 cut(s) 1546
TspGWI ACGGA 2 cut(s) 798, 839
TspMI CCCGGG 1 cut(s) 845
TspRI CASTG 6 cut(s) 189, 592, 1045, 1639, 1806, 1933
Tth111I GACNNNGTC 2 cut(s) 336, 1791
Van91I CCANNNNNTGG 1 cut(s) 1192
VneI GTGCAC 1 cut(s) 180
VspI ATTAAT 2 cut(s) 33, 2121
XapI RAATTY 2 cut(s) 7, 2282
XmaI CCCGGG 1 cut(s) 845
XmiI GTMKAC 4 cut(s) 331, 796, 1561, 1786
XmnI GAANNNNTTC 1 cut(s) 2286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.