AT2G24680

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001490
Physical Location & Seq
Reverse (-)
10493263 .. 10497009
3747 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G24680.3

Sequence Viewer

Length: 1341 bp
ATGGTGCTAAATTCGTCAGATTTAGGACCTAGCCGCTGTGATATTCGAGATTTACCAGCTCCGAGCAGCACCAATGATCAGGGCAAGACCGAACTTGCGAGGAAGAAGAAAGTGAAGAGGAGCAATACAGAAATAGAAGCTGATGCTTCTTCCTCCGACAACTCTTGTTTTGTGGCCCTTGTCACGGCTTCGAATCTACGCAAAGATGCTCTGTATCTTCCACAAGATTTAACAAGTTCAGTTGGTCTTGAAAGGAAATACCGTGAGATTGTTGTAACAGATGAAAGGGAAAGAAGATCATGGGCGTTGGATCTGAGGTTCAACAAATCATCTGATACTTTCTACATAAGCCGAGGTTGGAGGAGTTTCTGTGATGAAAACGGTAAAAAGCCAGGAGGTGTCTTTGTTTTCAAACTAGTTGGAAACAGGGAAACTCCGGTGCTTAGTTTCTGTTCCACTGAATCTATCAACGATGGAACACAAGGACATAAGAACAACAAATACAATTGTATGGAGCTGAAGAGCAAGAAGAAGCGTATGAGATGTAGAGATTCAACTTCACCAAGCCAAAACCGGTTTATGACATTAACACTTACTCATGACAACCTCATAAAAAGTAGACGGTATCTTCCGTTGTCATTCACAAGAGATAATGGCTTGGACAAACCTGGGATGATTTTTCTGTTGGGCAAAACTGGTAGAAAGTGGGAGGCAAATCTTCTAAGGGAAGCCTCAGGAAGAATTGTTTTGACGGGAAAGGGCTGGAAAGAGTTCGCCATGGCAAACGGCTTAAAAAGCGGTGAATTGTTCACGTTGGAGGCAATTTTGGAGAAGGGAACTCCTATGCTCAGTTTGGTCAATACACAGTCCACAAATTATAGAAGCCAACAAGGAGAATGTTCAAGAGATAGCGAGAAGGAGTCGTCGATTTGTGCTGAACCTAGTAGAGGAAACAAGAAGTGGAAAGCTACTAACAACAGAAAGGAGAGGAGAGACTCGTCGTCAGCAATCCAAAATCGATATGTGACATTAACACTCACACCAGAAGATGTCAGAGCCTGTACACTAATTCTTCCGAGTCAATTCATGAAAGCTAATGGCATCAACAAGCTTGGGAAGAAAACTTTGTTGGGTCAAAACAGAAAGAAGTGGTTTGCATATCTACTGTCAAAAAGTGGATTTGTGGCACTGGGATCTGGTTGGAAAGGTTTCTGTGAAGCTAATGGCGTTAAGACAGGAGAATCATTCAATTTGGAATATATTGATGAACAAGACACAACTCCTGTGTTTAAGTTCTGTTCCAACTCCGTAGAGTATGTAAAGTTCACAAGCCTTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

446

Amino Acids

50.21

Weight (kDa)

9.58

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 91 - 184 5.1e-19 B3 DNA binding domain
B3 PF02362 239 - 332 4.2e-20 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 619
AciI CCGC 2 cut(s) 34, 798
AclWI GGATC 2 cut(s) 318, 1201
AcsI RAATTY 1 cut(s) 10
AcuI CTGAAG 1 cut(s) 539
AfaI GTAC 1 cut(s) 1063
AfiI CCNNNNNNNGG 2 cut(s) 184, 947
AgeI ACCGGT 1 cut(s) 573
AgsI TTSAA 6 cut(s) 251, 322, 412, 555, 903, 1249
AhdI GACNNNNNGTC 1 cut(s) 1000
AhlI ACTAGT 1 cut(s) 415
AjnI CCWGG 2 cut(s) 391, 667
AjuI GAANNNNNNNTTGG 2 cut(s) 1112, 1144
AloI GAACNNNNNNTCC 2 cut(s) 302, 334
AluBI AGCT 7 cut(s) 59, 140, 517, 968, 1094, 1111, 1220
AluI AGCT 7 cut(s) 59, 140, 517, 968, 1094, 1111, 1220
Alw26I GTCTC 1 cut(s) 987
AlwI GGATC 2 cut(s) 318, 1201
AlwNI CAGNNNCTG 1 cut(s) 1059
AoxI GGCC 1 cut(s) 174
ApeKI GCWGC 1 cut(s) 66
ApoI RAATTY 1 cut(s) 10
AsiGI ACCGGT 1 cut(s) 573
Asp700I GAANNNNTTC 2 cut(s) 770, 1208
AspS9I GGNCC 2 cut(s) 26, 175
AsuHPI GGTGA 2 cut(s) 552, 812
AsuII TTCGAA 1 cut(s) 191
AvaII GGWCC 1 cut(s) 26
AxyI CCTNAGG 1 cut(s) 733
BbvI GCAGC 1 cut(s) 78
BccI CCATC 1 cut(s) 467
BceAI ACGGC 2 cut(s) 201, 802
BciT130I CCWGG 2 cut(s) 393, 669
BclI TGATCA 1 cut(s) 76
BcoDI GTCTC 1 cut(s) 987
BcuI ACTAGT 1 cut(s) 415
BfaI CTAG 3 cut(s) 30, 416, 942
BisI GCNGC 2 cut(s) 34, 67
BlsI GCNGC 2 cut(s) 35, 68
Bme1390I CCNGG 2 cut(s) 393, 669
Bme18I GGWCC 1 cut(s) 26
BmeRI GACNNNNNGTC 1 cut(s) 1000
BmgT120I GGNCC 2 cut(s) 26, 175
BmrFI CCNGG 2 cut(s) 393, 669
BmrI ACTGGG 1 cut(s) 1199
BmsI GCATC 3 cut(s) 133, 196, 1110
BmuI ACTGGG 1 cut(s) 1199
Bpu14I TTCGAA 1 cut(s) 191
Bsa29I ATCGAT 1 cut(s) 1018
BsaJI CCNNGG 3 cut(s) 352, 668, 777
BsaWI WCCGGW 2 cut(s) 436, 573
BsaXI ACNNNNNCTCC 2 cut(s) 355, 385
Bsc4I CCNNNNNNNGG 2 cut(s) 184, 947
Bse118I RCCGGY 1 cut(s) 573
Bse1I ACTGG 2 cut(s) 700, 1194
Bse21I CCTNAGG 1 cut(s) 733
BseBI CCWGG 2 cut(s) 393, 669
BseCI ATCGAT 1 cut(s) 1018
BseDI CCNNGG 3 cut(s) 352, 668, 777
BseGI GGATG 1 cut(s) 678
BseLI CCNNNNNNNGG 2 cut(s) 184, 947
BseMII CTCAG 3 cut(s) 305, 747, 862
BseNI ACTGG 2 cut(s) 700, 1194
BseRI GAGGAG 3 cut(s) 133, 376, 1003
BseXI GCAGC 1 cut(s) 78
BshFI GGCC 1 cut(s) 176
BshTI ACCGGT 1 cut(s) 573
BshVI ATCGAT 1 cut(s) 1018
BsiSI CCGG 2 cut(s) 437, 574
BslI CCNNNNNNNGG 2 cut(s) 184, 947
BsmAI GTCTC 1 cut(s) 987
BsnI GGCC 1 cut(s) 176
Bsp119I TTCGAA 1 cut(s) 191
Bsp1407I TGTACA 1 cut(s) 1061
Bsp143I GATC 4 cut(s) 76, 296, 310, 1193
Bsp19I CCATGG 1 cut(s) 777
BspACI CCGC 2 cut(s) 34, 798
BspANI GGCC 1 cut(s) 176
BspCNI CTCAG 3 cut(s) 306, 746, 861
BspDI ATCGAT 1 cut(s) 1018
BspHI TCATGA 2 cut(s) 598, 1086
BspPI GGATC 2 cut(s) 318, 1201
BspQI GCTCTTC 1 cut(s) 515
BspT104I TTCGAA 1 cut(s) 191
BsrFI RCCGGY 1 cut(s) 573
BsrGI TGTACA 1 cut(s) 1061
BsrI ACTGG 2 cut(s) 700, 1194
BssAI RCCGGY 1 cut(s) 573
BssECI CCNNGG 3 cut(s) 352, 668, 777
BssMI GATC 4 cut(s) 76, 296, 310, 1193
BssT1I CCWWGG 1 cut(s) 777
Bst2UI CCWGG 2 cut(s) 393, 669
Bst4CI ACNGT 5 cut(s) 263, 383, 624, 867, 1167
Bst6I CTCTTC 2 cut(s) 110, 515
BstAUI TGTACA 1 cut(s) 1061
BstBI TTCGAA 1 cut(s) 191
BstDEI CTNAG 5 cut(s) 314, 443, 722, 733, 848
BstDSI CCRYGG 1 cut(s) 777
BstENI CCTNNNNNAGG 1 cut(s) 945
BstF5I GGATG 1 cut(s) 678
BstKTI GATC 4 cut(s) 79, 299, 313, 1196
BstMAI GTCTC 1 cut(s) 987
BstMBI GATC 4 cut(s) 76, 296, 310, 1193
BstMWI GCNNNNNNNGC 1 cut(s) 795
BstNI CCWGG 2 cut(s) 393, 669
BstSCI CCNGG 2 cut(s) 391, 667
BstV1I GCAGC 1 cut(s) 78
BstX2I RGATCY 2 cut(s) 310, 1193
BstYI RGATCY 2 cut(s) 310, 1193
Bsu15I ATCGAT 1 cut(s) 1018
Bsu36I CCTNAGG 1 cut(s) 733
BsuRI GGCC 1 cut(s) 176
BsuTUI ATCGAT 1 cut(s) 1018
BtgI CCRYGG 1 cut(s) 777
BtsCI GGATG 1 cut(s) 678
BtsIMutI CAGTG 2 cut(s) 456, 1187
CaiI CAGNNNCTG 1 cut(s) 1059
CciI TCATGA 2 cut(s) 598, 1086
Cfr10I RCCGGY 1 cut(s) 573
Cfr13I GGNCC 2 cut(s) 26, 175
ClaI ATCGAT 1 cut(s) 1018
Csp6I GTAC 1 cut(s) 1062
CspAI ACCGGT 1 cut(s) 573
CviAII CATG 4 cut(s) 300, 599, 778, 1087
CviQI GTAC 1 cut(s) 1062
DdeI CTNAG 5 cut(s) 314, 443, 722, 733, 848
DpnI GATC 4 cut(s) 78, 298, 312, 1195
DpnII GATC 4 cut(s) 76, 296, 310, 1193
DriI GACNNNNNGTC 1 cut(s) 1000
Eam1104I CTCTTC 2 cut(s) 110, 515
Eam1105I GACNNNNNGTC 1 cut(s) 1000
EarI CTCTTC 2 cut(s) 110, 515
Eco130I CCWWGG 1 cut(s) 777
Eco47I GGWCC 1 cut(s) 26
Eco57I CTGAAG 1 cut(s) 539
Eco81I CCTNAGG 1 cut(s) 733
EcoNI CCTNNNNNAGG 1 cut(s) 945
EcoO109I RGGNCCY 1 cut(s) 26
EcoRII CCWGG 2 cut(s) 391, 667
EcoT14I CCWWGG 1 cut(s) 777
ErhI CCWWGG 1 cut(s) 777
FaeI CATG 4 cut(s) 303, 602, 781, 1090
FalI AAGNNNNNCTT 1 cut(s) 1321
FatI CATG 4 cut(s) 299, 598, 777, 1086
FbaI TGATCA 1 cut(s) 76
FblI GTMKAC 1 cut(s) 619
Fnu4HI GCNGC 2 cut(s) 34, 67
FokI GGATG 1 cut(s) 685
Fsp4HI GCNGC 2 cut(s) 34, 67
FspBI CTAG 3 cut(s) 30, 416, 942
GluI GCNGC 2 cut(s) 34, 67
HaeIII GGCC 1 cut(s) 176
HapII CCGG 2 cut(s) 437, 574
Hin1II CATG 4 cut(s) 303, 602, 781, 1090
HindIII AAGCTT 1 cut(s) 1109
HinfI GANTC 7 cut(s) 193, 461, 551, 920, 995, 1078, 1241
HpaII CCGG 2 cut(s) 437, 574
HphI GGTGA 2 cut(s) 552, 812
Hpy166II GTNNAC 5 cut(s) 620, 810, 870, 1064, 1326
Hpy188I TCNGA 7 cut(s) 19, 63, 157, 315, 334, 1055, 1077
Hpy188III TCNNGA 6 cut(s) 47, 248, 599, 735, 903, 1087
Hpy8I GTNNAC 5 cut(s) 620, 810, 870, 1064, 1326
Hpy99I CGWCG 2 cut(s) 928, 1003
HpyAV CCTTC 2 cut(s) 826, 910
HpyCH4III ACNGT 5 cut(s) 263, 383, 624, 867, 1167
HpyCH4IV ACGT 1 cut(s) 812
HpyCH4V TGCA 1 cut(s) 1157
HpyF10VI GCNNNNNNNGC 1 cut(s) 795
HpyF3I CTNAG 5 cut(s) 314, 443, 722, 733, 848
HpySE526I ACGT 1 cut(s) 812
Hsp92II CATG 4 cut(s) 303, 602, 781, 1090
Ksp22I TGATCA 1 cut(s) 76
Kzo9I GATC 4 cut(s) 76, 296, 310, 1193
LguI GCTCTTC 1 cut(s) 515
LmnI GCTCC 3 cut(s) 64, 120, 514
Lsp1109I GCAGC 1 cut(s) 78
LweI GCATC 3 cut(s) 133, 196, 1110
MaeI CTAG 3 cut(s) 30, 416, 942
MaeII ACGT 1 cut(s) 812
MaeIII GTNAC 3 cut(s) 181, 274, 1024
MalI GATC 4 cut(s) 78, 298, 312, 1195
MboI GATC 4 cut(s) 76, 296, 310, 1193
MfeI CAATTG 1 cut(s) 505
MflI RGATCY 2 cut(s) 310, 1193
MluCI AATT 9 cut(s) 10, 505, 741, 803, 822, 874, 1068, 1082, 1249
MlyI GAGTC 3 cut(s) 929, 989, 1087
MmeI TCCRAC 7 cut(s) 180, 288, 338, 400, 795, 1181, 1326
MroXI GAANNNNTTC 2 cut(s) 770, 1208
MseI TTAA 7 cut(s) 230, 587, 791, 1031, 1230, 1290, 1339
MspA1I CMGCKG 1 cut(s) 36
MspI CCGG 2 cut(s) 437, 574
MspR9I CCNGG 2 cut(s) 393, 669
MunI CAATTG 1 cut(s) 505
MvaI CCWGG 2 cut(s) 393, 669
MwoI GCNNNNNNNGC 1 cut(s) 795
NcoI CCATGG 1 cut(s) 777
NdeII GATC 4 cut(s) 76, 296, 310, 1193
NlaIII CATG 4 cut(s) 303, 602, 781, 1090
NmeAIII GCCGAG 1 cut(s) 377
NmuCI GTSAC 2 cut(s) 181, 1024
NspV TTCGAA 1 cut(s) 191
PagI TCATGA 2 cut(s) 598, 1086
PciSI GCTCTTC 1 cut(s) 515
PdmI GAANNNNTTC 2 cut(s) 770, 1208
PfeI GAWTC 4 cut(s) 193, 461, 551, 1241
PinAI ACCGGT 1 cut(s) 573
PkrI GCNGC 2 cut(s) 35, 68
PleI GAGTC 3 cut(s) 928, 989, 1086
PpsI GAGTC 3 cut(s) 928, 989, 1086
PpuMI RGGWCCY 1 cut(s) 26
Psp5II RGGWCCY 1 cut(s) 26
Psp6I CCWGG 2 cut(s) 391, 667
PspGI CCWGG 2 cut(s) 391, 667
PspPI GGNCC 2 cut(s) 26, 175
PspPPI RGGWCCY 1 cut(s) 26
PsrI GAACNNNNNNTAC 4 cut(s) 485, 517, 1307, 1339
PstNI CAGNNNCTG 1 cut(s) 1059
PsuI RGATCY 2 cut(s) 310, 1193
RsaI GTAC 1 cut(s) 1063
RsaNI GTAC 1 cut(s) 1062
SapI GCTCTTC 1 cut(s) 515
SaqAI TTAA 7 cut(s) 230, 587, 791, 1031, 1230, 1290, 1339
SatI GCNGC 2 cut(s) 34, 67
Sau3AI GATC 4 cut(s) 76, 296, 310, 1193
Sau96I GGNCC 2 cut(s) 26, 175
SchI GAGTC 3 cut(s) 929, 989, 1087
ScrFI CCNGG 2 cut(s) 393, 669
SfaNI GCATC 3 cut(s) 133, 196, 1110
SfuI TTCGAA 1 cut(s) 191
SinI GGWCC 1 cut(s) 26
SpeI ACTAGT 1 cut(s) 415
Sse9I AATT 9 cut(s) 10, 505, 741, 803, 822, 874, 1068, 1082, 1249
SsiI CCGC 2 cut(s) 34, 798
SspMI CTAG 3 cut(s) 30, 416, 942
StyD4I CCNGG 2 cut(s) 391, 667
StyI CCWWGG 1 cut(s) 777
TaaI ACNGT 5 cut(s) 263, 383, 624, 867, 1167
TaiI ACGT 1 cut(s) 815
TaqI TCGA 4 cut(s) 46, 191, 926, 1018
TaqII GACCGA 1 cut(s) 104
TasI AATT 9 cut(s) 10, 505, 741, 803, 822, 874, 1068, 1082, 1249
TatI WGTACW 1 cut(s) 1061
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 4 cut(s) 193, 461, 551, 1241
Tru1I TTAA 7 cut(s) 230, 587, 791, 1031, 1230, 1290, 1339
Tru9I TTAA 7 cut(s) 230, 587, 791, 1031, 1230, 1290, 1339
TscAI CASTG 2 cut(s) 463, 1194
TseFI GTSAC 2 cut(s) 181, 1024
TseI GCWGC 1 cut(s) 66
Tsp45I GTSAC 2 cut(s) 181, 1024
TspDTI ATGAA 5 cut(s) 297, 390, 1075, 1103, 1281
TspGWI ACGGA 2 cut(s) 621, 1297
TspRI CASTG 2 cut(s) 463, 1194
VpaK11BI GGWCC 1 cut(s) 26
XagI CCTNNNNNAGG 1 cut(s) 945
XapI RAATTY 1 cut(s) 10
XmiI GTMKAC 1 cut(s) 619
XmnI GAANNNNTTC 2 cut(s) 770, 1208
XspI CTAG 3 cut(s) 30, 416, 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.