AT4G31650

BEST Arabidopsis thaliana protein match is transcriptional factor B3 family protein (TAIR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001490
Physical Location & Seq
Forward (+)
15330993 .. 15333553
2561 bp
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UTR
Exon/CDS
Intron
AT4G31650.2

Sequence Viewer

Length: 1482 bp
ATGAACAACCCGACACTCTTTTCTCCTAAAAGTCCACACTTTTTCCAGCCTCTTCTTCCCGGTTTCAAGAGCCACATCAACATTCCTGTGAAGTTCTTCTCCAAGTATATCAAGGGGAAACACGAAGGAAAGACCGTAAAATTGAGATCAGATTCTTCGAAGAGAACATGGAAAGTGAAGATTGAGGGCCATACGCTCACCGACGGTTGGAAAGAGTTTGTGGAGGCACATGATCTTCGAATCAGTGACTTCGTCATCTTCAAACACAAAGGAGACATGTTCTTCGATGTCACTGCTTTAGGATCCAGTTGCTGGGAATCTTCAAGAAAGGAGAAAGAAATTGAGGAGAATATAGAAACAGAACCCGATCAGTTTTCATCAGATCTAACATGTTTTAGCCAATCTGTGACCACTTCAAACCTAATGAGAGATTCAGTGGGTGTGCCCAGAGATTTCGCAAAGCGATATGGTTTCAACATAGGAAGGCATGAGATAGTTTTGATGAACGAAGAAGGAAAGCCATGGGAATCAGAAGTTATAAGTTATAAGTCTGGTAAAGTTATTGTAGCTGGAGGTTGGAAAAGTCTCTGCACTGAAAGTAAATTAGAAGTTGGAGATTCTTGTACATTTAAACTGCTTCACAACGCCAAAATGCCCGTGTTTCGGCTCTGCAGCAAGCTCCCAAAGGCTGGAGCAGAGGCTCGGCCACTCAAGAGAGCTCGAGTCCAAAGATGGAGCCAAGAGAGTAGATCGAAGCATGACGTGAGGGAGAAGATAGCTGAAGAAGGAGAACCTTCTCGTCGCAATCGCACATCTAACAAGTCTACTGGTGATCAAGGAAAGTTGCAGCAAACGCAATCTTGCTTTATCTTTGATCATGTGGCTAAGTCTCTTCCAAGGAATTTTGTTAGGTCCGATGGTCTGATTAAAGGAAGTAACAAGATTGTTCTAATGAATGAAGGAGCAAGGACATGGACGCTTATTCTGAAGTTTAGGGATTCACGTAGATCGTTTTACAGTAGGGGTGGCTGGAGAAGTTTCTGCCGTGAGAATGGGCTTAAACCTGGAGATTCTGTCACGTTTAAACTGGAGAGTAGCAACACAAAAACGCCTCTACTCCTTTTCTCCATTGCAGAATCAAAGAGTGCTTCCACAAAAGATAGGACCCGATTTCTGACACTAACTGTCACACCATCCAGTATCCGCAGTTCCAGACTTTATCTTTCGAAGAAGTTTATTAGTGTTAACAAGATGGAAAGGGCAGGAGGGAAGAAGATAACTCTATTGGACAAGAATGGAGAAGAGTGGCCAGTGACCTTACTGATGGATAAAAGATATACAAGGATGGTTTTGGGTTCAGGGTTGAGAGAATTCTGCAATGCTATTGGCGTTAAGGCATATGAATCTGTGGTGTTGGAGTTGGTTTGGGAAGAGACAACAACTCTTCCTATGTTCAAATTCTGCTCCAAGATCAAGACTTGA

Protein Analysis

493

Amino Acids

56.23

Weight (kDa)

9.76

Isoelectric Point (pI)

52.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 25 - 93 7.6e-13 B3 DNA binding domain
B3 PF02362 152 - 246 4.5e-21 B3 DNA binding domain
B3 PF02362 352 - 443 9.6e-23 B3 DNA binding domain
B3 PF02362 461 - 560 7.4e-22 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 539, 546
AccB7I CCANNNNNTGG 2 cut(s) 312, 689
AccI GTMKAC 1 cut(s) 824
AciI CCGC 1 cut(s) 1204
AclWI GGATC 2 cut(s) 297, 310
AcoI YGGCCR 2 cut(s) 704, 1307
AcsI RAATTY 3 cut(s) 901, 1370, 1457
AcuI CTGAAG 2 cut(s) 801, 1007
AfaI GTAC 1 cut(s) 625
AfiI CCNNNNNNNGG 4 cut(s) 207, 312, 663, 689
AflIII ACRYGT 2 cut(s) 276, 389
AgsI TTSAA 6 cut(s) 67, 262, 324, 417, 475, 1456
AjiI CACGTC 1 cut(s) 763
AjnI CCWGG 1 cut(s) 1063
AluBI AGCT 4 cut(s) 569, 679, 719, 779
AluI AGCT 4 cut(s) 569, 679, 719, 779
Alw21I GWGCWC 1 cut(s) 721
Alw26I GTCTC 4 cut(s) 267, 590, 894, 1427
AlwI GGATC 2 cut(s) 297, 310
AlwNI CAGNNNCTG 1 cut(s) 312
Ama87I CYCGRG 1 cut(s) 720
AoxI GGCC 3 cut(s) 187, 704, 1307
ApeKI GCWGC 2 cut(s) 672, 847
ApoI RAATTY 3 cut(s) 901, 1370, 1457
ArsI GACNNNNNNTTYG 2 cut(s) 266, 298
Asp700I GAANNNNTTC 1 cut(s) 95
AspS9I GGNCC 3 cut(s) 187, 912, 1164
AsuC2I CCSGG 1 cut(s) 60
AsuHPI GGTGA 2 cut(s) 190, 842
AsuII TTCGAA 3 cut(s) 158, 238, 1226
AvaI CYCGRG 1 cut(s) 720
AvaII GGWCC 2 cut(s) 912, 1164
BaeGI GKGCMC 1 cut(s) 447
BalI TGGCCA 1 cut(s) 1309
BamHI GGATCC 1 cut(s) 302
BanII GRGCYC 1 cut(s) 721
Bbv12I GWGCWC 1 cut(s) 721
BbvI GCAGC 2 cut(s) 684, 859
BccI CCATC 6 cut(s) 726, 911, 1201, 1246, 1318, 1339
BceAI ACGGC 1 cut(s) 1029
BcgI CGANNNNNNTGC 2 cut(s) 275, 309
BciT130I CCWGG 1 cut(s) 1065
BciVI GTATCC 1 cut(s) 1211
BclI TGATCA 2 cut(s) 832, 874
BcnI CCSGG 1 cut(s) 60
BcoDI GTCTC 4 cut(s) 267, 590, 894, 1427
BfmI CTRYAG 1 cut(s) 670
BfuI GTATCC 1 cut(s) 1211
BglII AGATCT 1 cut(s) 382
BisI GCNGC 2 cut(s) 673, 848
BlsI GCNGC 2 cut(s) 674, 849
Bme1390I CCNGG 2 cut(s) 60, 1065
Bme18I GGWCC 2 cut(s) 912, 1164
BmeT110I CYCGRG 1 cut(s) 720
BmgBI CACGTC 1 cut(s) 763
BmgT120I GGNCC 3 cut(s) 187, 912, 1164
BmiI GGNNCC 3 cut(s) 304, 737, 1166
BmrFI CCNGG 2 cut(s) 60, 1065
BpmI CTGGAG 5 cut(s) 591, 711, 1051, 1086, 1109
Bpu14I TTCGAA 3 cut(s) 158, 238, 1226
BpuEI CTTGAG 1 cut(s) 695
BpuMI CCSGG 1 cut(s) 60
BsaAI YACGTR 1 cut(s) 1004
BsaJI CCNNGG 2 cut(s) 521, 896
BsaXI ACNNNNNCTCC 2 cut(s) 1059, 1089
Bsc4I CCNNNNNNNGG 4 cut(s) 207, 312, 663, 689
Bse1I ACTGG 5 cut(s) 306, 832, 1092, 1197, 1310
Bse3DI GCAATG 2 cut(s) 1128, 1384
BseBI CCWGG 1 cut(s) 1065
BseDI CCNNGG 2 cut(s) 521, 896
BseGI GGATG 2 cut(s) 1193, 1350
BseLI CCNNNNNNNGG 4 cut(s) 207, 312, 663, 689
BseMI GCAATG 2 cut(s) 1128, 1384
BseNI ACTGG 5 cut(s) 306, 832, 1092, 1197, 1310
BseRI GAGGAG 1 cut(s) 359
BseSI GKGCMC 1 cut(s) 447
BseXI GCAGC 2 cut(s) 684, 859
BseYI CCCAGC 1 cut(s) 312
BsgI GTGCAG 1 cut(s) 574
BshFI GGCC 3 cut(s) 189, 706, 1309
BsiHKAI GWGCWC 1 cut(s) 721
BsiHKCI CYCGRG 1 cut(s) 720
BsiSI CCGG 1 cut(s) 60
BslI CCNNNNNNNGG 4 cut(s) 207, 312, 663, 689
BsmAI GTCTC 4 cut(s) 267, 590, 894, 1427
BsnI GGCC 3 cut(s) 189, 706, 1309
BsoBI CYCGRG 1 cut(s) 720
Bsp119I TTCGAA 3 cut(s) 158, 238, 1226
Bsp1286I GDGCHC 2 cut(s) 447, 721
Bsp1407I TGTACA 1 cut(s) 623
Bsp19I CCATGG 1 cut(s) 521
BspACI CCGC 1 cut(s) 1204
BspANI GGCC 3 cut(s) 189, 706, 1309
BspLI GGNNCC 3 cut(s) 304, 737, 1166
BspMAI CTGCAG 1 cut(s) 674
BspPI GGATC 2 cut(s) 297, 310
BspT104I TTCGAA 3 cut(s) 158, 238, 1226
BsrDI GCAATG 2 cut(s) 1128, 1384
BsrGI TGTACA 1 cut(s) 623
BsrI ACTGG 5 cut(s) 306, 832, 1092, 1197, 1310
BssECI CCNNGG 2 cut(s) 521, 896
BssT1I CCWWGG 2 cut(s) 521, 896
Bst2UI CCWGG 1 cut(s) 1065
Bst4CI ACNGT 4 cut(s) 136, 206, 1019, 1186
Bst6I CTCTTC 6 cut(s) 57, 155, 897, 1296, 1425, 1449
BstAUI TGTACA 1 cut(s) 623
BstBAI YACGTR 1 cut(s) 1004
BstBI TTCGAA 3 cut(s) 158, 238, 1226
BstC8I GCNNGC 1 cut(s) 677
BstDEI CTNAG 1 cut(s) 885
BstDSI CCRYGG 1 cut(s) 521
BstF5I GGATG 2 cut(s) 1193, 1350
BstMAI GTCTC 4 cut(s) 267, 590, 894, 1427
BstMWI GCNNNNNNNGC 1 cut(s) 853
BstNI CCWGG 1 cut(s) 1065
BstNSI RCATGY 2 cut(s) 280, 393
BstSCI CCNGG 2 cut(s) 58, 1063
BstSFI CTRYAG 1 cut(s) 670
BstSLI GKGCMC 1 cut(s) 447
BstV1I GCAGC 2 cut(s) 684, 859
BstX2I RGATCY 2 cut(s) 302, 382
BstYI RGATCY 2 cut(s) 302, 382
BsuI GTATCC 1 cut(s) 1211
BsuRI GGCC 3 cut(s) 189, 706, 1309
BtgI CCRYGG 1 cut(s) 521
BtrI CACGTC 1 cut(s) 763
BtsCI GGATG 2 cut(s) 1193, 1350
BtsI GCAGTG 1 cut(s) 291
BtsIMutI CAGTG 5 cut(s) 250, 291, 441, 591, 1317
Cac8I GCNNGC 1 cut(s) 677
CaiI CAGNNNCTG 1 cut(s) 312
Cfr13I GGNCC 3 cut(s) 187, 912, 1164
CseI GACGC 1 cut(s) 985
Csp6I GTAC 1 cut(s) 624
CviAII CATG 9 cut(s) 168, 230, 277, 390, 488, 522, 758, 878, 972
CviQI GTAC 1 cut(s) 624
DdeI CTNAG 1 cut(s) 885
DraI TTTAAA 2 cut(s) 631, 1084
EaeI YGGCCR 2 cut(s) 704, 1307
Eam1104I CTCTTC 6 cut(s) 57, 155, 897, 1296, 1425, 1449
EarI CTCTTC 6 cut(s) 57, 155, 897, 1296, 1425, 1449
Ecl136II GAGCTC 1 cut(s) 719
Eco130I CCWWGG 2 cut(s) 521, 896
Eco24I GRGCYC 1 cut(s) 721
Eco47I GGWCC 2 cut(s) 912, 1164
Eco53kI GAGCTC 1 cut(s) 719
Eco57I CTGAAG 2 cut(s) 801, 1007
Eco88I CYCGRG 1 cut(s) 720
EcoICRI GAGCTC 1 cut(s) 719
EcoO109I RGGNCCY 1 cut(s) 1164
EcoRI GAATTC 1 cut(s) 1370
EcoRII CCWGG 1 cut(s) 1063
EcoT14I CCWWGG 2 cut(s) 521, 896
EcoT38I GRGCYC 1 cut(s) 721
ErhI CCWWGG 2 cut(s) 521, 896
FaeI CATG 9 cut(s) 171, 233, 280, 393, 491, 525, 761, 881, 975
FatI CATG 9 cut(s) 167, 229, 276, 389, 487, 521, 757, 877, 971
FauNDI CATATG 1 cut(s) 1399
FbaI TGATCA 2 cut(s) 832, 874
FblI GTMKAC 1 cut(s) 824
Fnu4HI GCNGC 2 cut(s) 673, 848
FokI GGATG 2 cut(s) 1180, 1357
FriOI GRGCYC 1 cut(s) 721
Fsp4HI GCNGC 2 cut(s) 673, 848
GluI GCNGC 2 cut(s) 673, 848
GsaI CCCAGC 1 cut(s) 316
GsuI CTGGAG 5 cut(s) 591, 711, 1051, 1086, 1109
HaeIII GGCC 3 cut(s) 189, 706, 1309
HapII CCGG 1 cut(s) 60
HgaI GACGC 1 cut(s) 985
Hin1II CATG 9 cut(s) 171, 233, 280, 393, 491, 525, 761, 881, 975
HincII GTYRAC 1 cut(s) 1246
HindII GTYRAC 1 cut(s) 1246
HpaI GTTAAC 1 cut(s) 1246
HpaII CCGG 1 cut(s) 60
HphI GGTGA 2 cut(s) 190, 842
Hpy166II GTNNAC 3 cut(s) 35, 825, 1246
Hpy188I TCNGA 7 cut(s) 151, 382, 532, 916, 924, 987, 1176
Hpy188III TCNNGA 5 cut(s) 67, 324, 712, 1212, 1474
Hpy8I GTNNAC 3 cut(s) 35, 825, 1246
Hpy99I CGWCG 2 cut(s) 206, 804
HpyAV CCTTC 6 cut(s) 119, 477, 506, 779, 804, 953
HpyCH4III ACNGT 4 cut(s) 136, 206, 1019, 1186
HpyCH4IV ACGT 3 cut(s) 762, 1003, 1079
HpyCH4V TGCA 5 cut(s) 591, 672, 847, 1133, 1377
HpyF10VI GCNNNNNNNGC 1 cut(s) 853
HpyF3I CTNAG 1 cut(s) 885
HpySE526I ACGT 3 cut(s) 762, 1003, 1079
Hsp92II CATG 9 cut(s) 171, 233, 280, 393, 491, 525, 761, 881, 975
Ksp22I TGATCA 2 cut(s) 832, 874
KspAI GTTAAC 1 cut(s) 1246
LmnI GCTCC 5 cut(s) 684, 692, 735, 962, 1469
Lsp1109I GCAGC 2 cut(s) 684, 859
MaeII ACGT 3 cut(s) 762, 1003, 1079
MaeIII GTNAC 7 cut(s) 245, 289, 406, 935, 1075, 1186, 1312
MflI RGATCY 2 cut(s) 302, 382
MhlI GDGCHC 2 cut(s) 447, 721
MlsI TGGCCA 1 cut(s) 1309
MluCI AATT 6 cut(s) 140, 339, 602, 901, 1370, 1457
MluNI TGGCCA 1 cut(s) 1309
MlyI GAGTC 1 cut(s) 732
MmeI TCCRAC 4 cut(s) 188, 557, 592, 1395
MnlI CCTC 9 cut(s) 60, 178, 217, 337, 566, 691, 759, 1122, 1259
Mox20I TGGCCA 1 cut(s) 1309
MroXI GAANNNNTTC 1 cut(s) 95
MscI TGGCCA 1 cut(s) 1309
MseI TTAA 6 cut(s) 630, 927, 1059, 1083, 1245, 1392
MslI CAYNNNNRTG 1 cut(s) 86
Msp20I TGGCCA 1 cut(s) 1309
MspI CCGG 1 cut(s) 60
MspR9I CCNGG 2 cut(s) 60, 1065
MssI GTTTAAAC 1 cut(s) 1084
MvaI CCWGG 1 cut(s) 1065
MwoI GCNNNNNNNGC 1 cut(s) 853
NciI CCSGG 1 cut(s) 60
NcoI CCATGG 1 cut(s) 521
NdeI CATATG 1 cut(s) 1399
NlaIII CATG 9 cut(s) 171, 233, 280, 393, 491, 525, 761, 881, 975
NlaIV GGNNCC 3 cut(s) 304, 737, 1166
NmeAIII GCCGAG 1 cut(s) 682
NmuCI GTSAC 6 cut(s) 245, 289, 406, 1075, 1186, 1312
NspI RCATGY 2 cut(s) 280, 393
NspV TTCGAA 3 cut(s) 158, 238, 1226
PaeR7I CTCGAG 1 cut(s) 720
PciI ACATGT 2 cut(s) 276, 389
PdmI GAANNNNTTC 1 cut(s) 95
PflFI GACNNNGTC 1 cut(s) 251
PflMI CCANNNNNTGG 2 cut(s) 312, 689
PkrI GCNGC 2 cut(s) 674, 849
PleI GAGTC 1 cut(s) 731
PmeI GTTTAAAC 1 cut(s) 1084
PpsI GAGTC 1 cut(s) 731
Ppu21I YACGTR 1 cut(s) 1004
PpuMI RGGWCCY 1 cut(s) 1164
PscI ACATGT 2 cut(s) 276, 389
PsiI TTATAA 2 cut(s) 539, 546
Psp124BI GAGCTC 1 cut(s) 721
Psp5II RGGWCCY 1 cut(s) 1164
Psp6I CCWGG 1 cut(s) 1063
PspFI CCCAGC 1 cut(s) 312
PspGI CCWGG 1 cut(s) 1063
PspN4I GGNNCC 3 cut(s) 304, 737, 1166
PspPI GGNCC 3 cut(s) 187, 912, 1164
PspPPI RGGWCCY 1 cut(s) 1164
PspXI VCTCGAGB 1 cut(s) 720
PsrI GAACNNNNNNTAC 2 cut(s) 1192, 1224
PstI CTGCAG 1 cut(s) 674
PstNI CAGNNNCTG 1 cut(s) 312
PsuI RGATCY 2 cut(s) 302, 382
PsyI GACNNNGTC 1 cut(s) 251
RsaI GTAC 1 cut(s) 625
RsaNI GTAC 1 cut(s) 624
RseI CAYNNNNRTG 1 cut(s) 86
SacI GAGCTC 1 cut(s) 721
SaqAI TTAA 6 cut(s) 630, 927, 1059, 1083, 1245, 1392
SatI GCNGC 2 cut(s) 673, 848
Sau96I GGNCC 3 cut(s) 187, 912, 1164
SchI GAGTC 1 cut(s) 732
ScrFI CCNGG 2 cut(s) 60, 1065
SduI GDGCHC 2 cut(s) 447, 721
SfcI CTRYAG 1 cut(s) 670
Sfr274I CTCGAG 1 cut(s) 720
SfuI TTCGAA 3 cut(s) 158, 238, 1226
SinI GGWCC 2 cut(s) 912, 1164
SlaI CTCGAG 1 cut(s) 720
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 2 cut(s) 710, 720
SmoI CTYRAG 2 cut(s) 710, 720
Sse9I AATT 6 cut(s) 140, 339, 602, 901, 1370, 1457
SsiI CCGC 1 cut(s) 1204
SstI GAGCTC 1 cut(s) 721
StyD4I CCNGG 2 cut(s) 58, 1063
StyI CCWWGG 2 cut(s) 521, 896
TaaI ACNGT 4 cut(s) 136, 206, 1019, 1186
TaiI ACGT 3 cut(s) 765, 1006, 1082
TaqI TCGA 6 cut(s) 158, 238, 285, 721, 752, 1226
TasI AATT 6 cut(s) 140, 339, 602, 901, 1370, 1457
TatI WGTACW 1 cut(s) 623
Tru1I TTAA 6 cut(s) 630, 927, 1059, 1083, 1245, 1392
Tru9I TTAA 6 cut(s) 630, 927, 1059, 1083, 1245, 1392
TscAI CASTG 5 cut(s) 250, 298, 441, 598, 1317
TseFI GTSAC 6 cut(s) 245, 289, 406, 1075, 1186, 1312
TseI GCWGC 2 cut(s) 672, 847
Tsp45I GTSAC 6 cut(s) 245, 289, 406, 1075, 1186, 1312
TspDTI ATGAA 6 cut(s) 17, 366, 518, 968, 972, 1416
TspRI CASTG 5 cut(s) 250, 298, 441, 598, 1317
Tth111I GACNNNGTC 1 cut(s) 251
Van91I CCANNNNNTGG 2 cut(s) 312, 689
VpaK11BI GGWCC 2 cut(s) 912, 1164
XapI RAATTY 3 cut(s) 901, 1370, 1457
XceI RCATGY 2 cut(s) 280, 393
XhoI CTCGAG 1 cut(s) 720
XmiI GTMKAC 1 cut(s) 824
XmnI GAANNNNTTC 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.