AT4G31615

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001490
Physical Location & Seq
Reverse (-)
15320026 .. 15322311
2286 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31615.1

Sequence Viewer

Length: 1464 bp
ATGGATGATCCAGCAATTTCCTCTCCAACCAACAAGCATTTCTTCGTCATAGTTCTTCATGGACACAAATCCAGTCCCATGATTCCTGCTGAGTTTTTTTCGACTTACGTGGAAGGTAAAAACCACCAGTCTACAAAACTGAAACTGACCTCGGACGCTTTCGATAGAACCTGGGAAGTGAAATTGAACGGCCGCAGATTCGCCGGCGGCTGGGAAAATTTCTCCACCGTACACTCTCTCCAAGATGACGACGTTGTGATTTTCAGGGAAATCGGGGACATGACCTTTCACGTCACAGCCTCCGGTCGCAGTTTCTGTAAGTTACAGTACATATCCTCTGACGAAGATAGGGCTGATGAAGATGATGCATGGAACGTGTATTCAGATTCCAAGAACATTGTGTCGAAGAAGAAGCCAAGAACAGAAGCAGAGTCTTCATCCAAAAACTATTATCTTGTTGCGCATGTCACGCCTTCGAACCTAAGCCGAGATAGATTGTATCTTATAAAGGCTTTTGCGAGGTCAAATGGCCTGAACGAGAGATGCTGTGAGATTGATCTAATGAATAAACATGGAAAATCATGGACACTTGGTCTGAGATACAGTACAGTCAATGATCAGGCTAGTATCAGTGGAGGCTGGAGAGACTTCTGCTTAGTAAATGGGCTAGAAGCTGGATCATTCTACAGGTTTAAGCTTGTCCAAAACGGGGAAAGGCCTTTACTACGGTTGTGTTACGACACTATTCCAGAAGAAGACTGTTACAAAGGAAACAGAAAAGTTAATCTTTCAGAGAGTGCTTCAATGAAGCAGGGCAAGTTTCTGACAATGACCTTTAAGCCTTACATGCTCAAGTCAGGTCAACTTCGTCTCTCGAGACCCTTTGCAATAGAGAATGAAATAAAGGAGGCAGAGGATATAACACTAGTGGACAAAAATGGAGGAAACTGGCCATCCTATGTAGCTTCTGGCGATGGTCAAGGAGGAAAGTATTATTTGGCAAAAGGTTGGAGAAGTTTTTGTGCAGCCAATAGGTTAAAGACGGGAGAGACCTTCACGTTGGAGTTTGTTCGAGGAGAAGGCACAACTCCAATGCTAAAGTTCTGCTCCAAGTCCAAGGACAAGATAGAACAAGTACCATTTGATGAAGGAGGGGAGACTCAAGAAGAAACAGAGACTAGGGTCAAAAAGAGAGCTCGAGTTTCTGCAGAAAGAGGATCTTCTCGCCGCAATCAGGCATCAAATAAACCCAATGCTGATCCACAAAACTTGCAGCCTGGGCAACCACATTGCTCAATCTCTGATCAAGTGCAAAAGGTGAAACAGATTATTGTTGACACTTTAACCGGGATAAAACGGATTCGGTCAGAGCTAGAAAGAAAGGGACAAAAGTTAGAAGCTTCGATACTGGAAATTGATGCTTTAGGGGAGAAAGTGTCGGAAATCAACAAAATCCTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

487

Amino Acids

55.04

Weight (kDa)

8.82

Isoelectric Point (pI)

37.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 24 - 98 3.7e-08 B3 DNA binding domain
B3 PF02362 153 - 245 7.9e-22 B3 DNA binding domain
B3 PF02362 274 - 372 4.8e-22 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 506
Acc16I TGCGCA 1 cut(s) 462
AccI GTMKAC 1 cut(s) 131
AciI CCGC 3 cut(s) 193, 207, 1228
AclWI GGATC 4 cut(s) 2, 685, 1225, 1253
AcoI YGGCCR 2 cut(s) 190, 950
AcsI RAATTY 1 cut(s) 217
AfaI GTAC 4 cut(s) 231, 329, 607, 1137
AfiI CCNNNNNNNGG 3 cut(s) 210, 709, 1234
AflIII ACRYGT 1 cut(s) 375
AgsI TTSAA 2 cut(s) 187, 804
AhdI GACNNNNNGTC 1 cut(s) 591
AhlI ACTAGT 1 cut(s) 925
AjiI CACGTC 1 cut(s) 292
AjnI CCWGG 2 cut(s) 170, 1276
AjuI GAANNNNNNNTTGG 2 cut(s) 23, 55
AluBI AGCT 6 cut(s) 674, 697, 965, 1196, 1372, 1400
AluI AGCT 6 cut(s) 674, 697, 965, 1196, 1372, 1400
Alw21I GWGCWC 1 cut(s) 1198
Alw26I GTCTC 6 cut(s) 639, 871, 875, 1043, 1151, 1169
AlwI GGATC 4 cut(s) 2, 685, 1225, 1253
AlwNI CAGNNNCTG 1 cut(s) 315
Ama87I CYCGRG 2 cut(s) 874, 1197
AoxI GGCC 4 cut(s) 190, 529, 716, 950
ApeKI GCWGC 2 cut(s) 1025, 1273
ApoI RAATTY 1 cut(s) 217
AspLEI GCGC 1 cut(s) 463
AsuC2I CCSGG 1 cut(s) 1348
AsuHPI GGTGA 1 cut(s) 1330
AsuII TTCGAA 1 cut(s) 476
AvaI CYCGRG 2 cut(s) 874, 1197
BalI TGGCCA 1 cut(s) 952
BanII GRGCYC 1 cut(s) 1198
BarI GAAGNNNNNNTAC 2 cut(s) 1389, 1421
BbsI GAAGAC 2 cut(s) 426, 762
Bbv12I GWGCWC 1 cut(s) 1198
BbvI GCAGC 2 cut(s) 1037, 1285
BccI CCATC 2 cut(s) 961, 968
BceAI ACGGC 1 cut(s) 205
BciT130I CCWGG 2 cut(s) 172, 1278
BclI TGATCA 2 cut(s) 616, 1303
BcnI CCSGG 1 cut(s) 1348
BcoDI GTCTC 6 cut(s) 639, 871, 875, 1043, 1151, 1169
BcuI ACTAGT 1 cut(s) 925
BfaI CTAG 5 cut(s) 624, 668, 926, 1179, 1373
BfmI CTRYAG 2 cut(s) 685, 1206
BisI GCNGC 5 cut(s) 193, 208, 1026, 1228, 1274
BlsI GCNGC 5 cut(s) 194, 209, 1027, 1229, 1275
Bme1390I CCNGG 3 cut(s) 172, 1278, 1348
BmeRI GACNNNNNGTC 1 cut(s) 591
BmeT110I CYCGRG 2 cut(s) 874, 1197
BmgBI CACGTC 1 cut(s) 292
BmrFI CCNGG 3 cut(s) 172, 1278, 1348
BmsI GCATC 4 cut(s) 355, 533, 1247, 1408
BoxI GACNNNNGTC 1 cut(s) 1181
BpiI GAAGAC 2 cut(s) 426, 762
BpmI CTGGAG 1 cut(s) 661
Bpu10I CCTNAGC 1 cut(s) 482
Bpu14I TTCGAA 1 cut(s) 476
BpuEI CTTGAG 3 cut(s) 836, 1146, 1442
BpuMI CCSGG 1 cut(s) 1348
BsaAI YACGTR 1 cut(s) 109
BsaI GGTCTC 2 cut(s) 871, 1043
BsaJI CCNNGG 4 cut(s) 150, 171, 1116, 1277
BsaWI WCCGGW 1 cut(s) 302
BsaXI ACNNNNNCTCC 4 cut(s) 222, 252, 1421, 1451
Bsc4I CCNNNNNNNGG 3 cut(s) 210, 709, 1234
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 4 cut(s) 72, 127, 953, 1413
Bse3DI GCAATG 1 cut(s) 1288
BseBI CCWGG 2 cut(s) 172, 1278
BseDI CCNNGG 4 cut(s) 150, 171, 1116, 1277
BseGI GGATG 3 cut(s) 10, 437, 953
BseLI CCNNNNNNNGG 3 cut(s) 210, 709, 1234
BseMI GCAATG 1 cut(s) 1288
BseMII CTCAG 2 cut(s) 81, 587
BseNI ACTGG 4 cut(s) 72, 127, 953, 1413
BseRI GAGGAG 1 cut(s) 1089
BseX3I CGGCCG 1 cut(s) 190
BseXI GCAGC 2 cut(s) 1037, 1285
BseYI CCCAGC 1 cut(s) 210
BsgI GTGCAG 1 cut(s) 1044
Bsh1285I CGRYCG 2 cut(s) 193, 307
BshFI GGCC 4 cut(s) 192, 531, 718, 952
BsiEI CGRYCG 2 cut(s) 193, 307
BsiHKAI GWGCWC 1 cut(s) 1198
BsiHKCI CYCGRG 2 cut(s) 874, 1197
BsiSI CCGG 3 cut(s) 204, 303, 1347
BslFI GGGAC 3 cut(s) 60, 290, 1398
BslI CCNNNNNNNGG 3 cut(s) 210, 709, 1234
BsmAI GTCTC 6 cut(s) 639, 871, 875, 1043, 1151, 1169
BsmBI CGTCTC 1 cut(s) 875
BsmFI GGGAC 3 cut(s) 60, 290, 1398
BsnI GGCC 4 cut(s) 192, 531, 718, 952
Bso31I GGTCTC 2 cut(s) 871, 1043
BsoBI CYCGRG 2 cut(s) 874, 1197
Bsp119I TTCGAA 1 cut(s) 476
Bsp1286I GDGCHC 1 cut(s) 1198
Bsp143I GATC 7 cut(s) 7, 556, 616, 677, 1217, 1258, 1303
BspACI CCGC 3 cut(s) 193, 207, 1228
BspANI GGCC 4 cut(s) 192, 531, 718, 952
BspCNI CTCAG 2 cut(s) 82, 588
BspMAI CTGCAG 1 cut(s) 1210
BspPI GGATC 4 cut(s) 2, 685, 1225, 1253
BspT104I TTCGAA 1 cut(s) 476
BspTNI GGTCTC 2 cut(s) 871, 1043
BsrDI GCAATG 1 cut(s) 1288
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 4 cut(s) 72, 127, 953, 1413
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 4 cut(s) 150, 171, 1116, 1277
BssMI GATC 7 cut(s) 7, 556, 616, 677, 1217, 1258, 1303
BssT1I CCWWGG 1 cut(s) 1116
Bst2UI CCWGG 2 cut(s) 172, 1278
Bst4CI ACNGT 6 cut(s) 229, 327, 605, 610, 729, 761
BstBAI YACGTR 1 cut(s) 109
BstBI TTCGAA 1 cut(s) 476
BstC8I GCNNGC 1 cut(s) 205
BstDEI CTNAG 4 cut(s) 90, 482, 596, 655
BstF5I GGATG 3 cut(s) 10, 437, 953
BstHHI GCGC 1 cut(s) 463
BstKTI GATC 7 cut(s) 10, 559, 619, 680, 1220, 1261, 1306
BstMAI GTCTC 6 cut(s) 639, 871, 875, 1043, 1151, 1169
BstMBI GATC 7 cut(s) 7, 556, 616, 677, 1217, 1258, 1303
BstMCI CGRYCG 2 cut(s) 193, 307
BstMWI GCNNNNNNNGC 3 cut(s) 469, 847, 1279
BstNI CCWGG 2 cut(s) 172, 1278
BstNSI RCATGY 2 cut(s) 467, 850
BstPAI GACNNNNGTC 1 cut(s) 1181
BstSCI CCNGG 3 cut(s) 170, 1276, 1346
BstSFI CTRYAG 2 cut(s) 685, 1206
BstV1I GCAGC 2 cut(s) 1037, 1285
BstV2I GAAGAC 2 cut(s) 426, 762
BstX2I RGATCY 1 cut(s) 1217
BstYI RGATCY 1 cut(s) 1217
BstZI CGGCCG 1 cut(s) 190
BsuRI GGCC 4 cut(s) 192, 531, 718, 952
BtgZI GCGATG 1 cut(s) 987
BtrI CACGTC 1 cut(s) 292
BtsCI GGATG 3 cut(s) 10, 437, 953
BtsIMutI CAGTG 1 cut(s) 637
Cac8I GCNNGC 1 cut(s) 205
CaiI CAGNNNCTG 1 cut(s) 315
CfoI GCGC 1 cut(s) 463
Cfr10I RCCGGY 1 cut(s) 203
CseI GACGC 1 cut(s) 164
Csp6I GTAC 4 cut(s) 230, 328, 606, 1136
CspCI CAANNNNNGTGG 2 cut(s) 1251, 1286
CviAII CATG 8 cut(s) 59, 79, 280, 369, 464, 572, 582, 847
CviQI GTAC 4 cut(s) 230, 328, 606, 1136
DdeI CTNAG 4 cut(s) 90, 482, 596, 655
DpnI GATC 7 cut(s) 9, 558, 618, 679, 1219, 1260, 1305
DpnII GATC 7 cut(s) 7, 556, 616, 677, 1217, 1258, 1303
DriI GACNNNNNGTC 1 cut(s) 591
EaeI YGGCCR 2 cut(s) 190, 950
EagI CGGCCG 1 cut(s) 190
Eam1105I GACNNNNNGTC 1 cut(s) 591
Ecl136II GAGCTC 1 cut(s) 1196
EclXI CGGCCG 1 cut(s) 190
Eco130I CCWWGG 1 cut(s) 1116
Eco147I AGGCCT 1 cut(s) 718
Eco24I GRGCYC 1 cut(s) 1198
Eco31I GGTCTC 2 cut(s) 871, 1043
Eco52I CGGCCG 1 cut(s) 190
Eco53kI GAGCTC 1 cut(s) 1196
Eco88I CYCGRG 2 cut(s) 874, 1197
EcoICRI GAGCTC 1 cut(s) 1196
EcoRII CCWGG 2 cut(s) 170, 1276
EcoT14I CCWWGG 1 cut(s) 1116
EcoT22I ATGCAT 1 cut(s) 370
EcoT38I GRGCYC 1 cut(s) 1198
ErhI CCWWGG 1 cut(s) 1116
Esp3I CGTCTC 1 cut(s) 875
FaeI CATG 8 cut(s) 62, 82, 283, 372, 467, 575, 585, 850
FalI AAGNNNNNCTT 6 cut(s) 26, 58, 771, 803, 1204, 1236
FaqI GGGAC 3 cut(s) 60, 290, 1398
FatI CATG 8 cut(s) 58, 78, 279, 368, 463, 571, 581, 846
FbaI TGATCA 2 cut(s) 616, 1303
FblI GTMKAC 1 cut(s) 131
Fnu4HI GCNGC 5 cut(s) 193, 208, 1026, 1228, 1274
FokI GGATG 3 cut(s) 17, 424, 940
FriOI GRGCYC 1 cut(s) 1198
Fsp4HI GCNGC 5 cut(s) 193, 208, 1026, 1228, 1274
FspBI CTAG 5 cut(s) 624, 668, 926, 1179, 1373
FspI TGCGCA 1 cut(s) 462
GlaI GCGC 1 cut(s) 462
GluI GCNGC 5 cut(s) 193, 208, 1026, 1228, 1274
GsaI CCCAGC 1 cut(s) 214
GsuI CTGGAG 1 cut(s) 661
HaeIII GGCC 4 cut(s) 192, 531, 718, 952
HapII CCGG 3 cut(s) 204, 303, 1347
HgaI GACGC 1 cut(s) 164
HhaI GCGC 1 cut(s) 463
Hin1II CATG 8 cut(s) 62, 82, 283, 372, 467, 575, 585, 850
Hin6I GCGC 1 cut(s) 461
HinP1I GCGC 1 cut(s) 461
HincII GTYRAC 2 cut(s) 863, 1336
HindII GTYRAC 2 cut(s) 863, 1336
HindIII AAGCTT 2 cut(s) 695, 1398
HinfI GANTC 6 cut(s) 82, 198, 386, 431, 1159, 1360
HpaII CCGG 3 cut(s) 204, 303, 1347
HphI GGTGA 1 cut(s) 1330
Hpy166II GTNNAC 5 cut(s) 132, 232, 863, 931, 1336
Hpy188I TCNGA 9 cut(s) 154, 340, 385, 597, 793, 825, 1303, 1369, 1441
Hpy188III TCNNGA 4 cut(s) 749, 874, 876, 1163
Hpy8I GTNNAC 5 cut(s) 132, 232, 863, 931, 1336
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 5 cut(s) 107, 483, 1063, 1073, 1142
HpyCH4III ACNGT 6 cut(s) 229, 327, 605, 610, 729, 761
HpyCH4IV ACGT 5 cut(s) 108, 252, 291, 375, 1058
HpyCH4V TGCA 6 cut(s) 368, 887, 1025, 1208, 1273, 1312
HpyF10VI GCNNNNNNNGC 3 cut(s) 469, 847, 1279
HpyF3I CTNAG 4 cut(s) 90, 482, 596, 655
HpySE526I ACGT 5 cut(s) 108, 252, 291, 375, 1058
Hsp92II CATG 8 cut(s) 62, 82, 283, 372, 467, 575, 585, 850
HspAI GCGC 1 cut(s) 461
KroI GCCGGC 1 cut(s) 203
KroNI GCCGGC 1 cut(s) 205
Ksp22I TGATCA 2 cut(s) 616, 1303
Kzo9I GATC 7 cut(s) 7, 556, 616, 677, 1217, 1258, 1303
LmnI GCTCC 1 cut(s) 1112
Lsp1109I GCAGC 2 cut(s) 1037, 1285
LweI GCATC 4 cut(s) 355, 533, 1247, 1408
MaeI CTAG 5 cut(s) 624, 668, 926, 1179, 1373
MaeII ACGT 5 cut(s) 108, 252, 291, 375, 1058
MaeIII GTNAC 5 cut(s) 292, 321, 466, 734, 761
MalI GATC 7 cut(s) 9, 558, 618, 679, 1219, 1260, 1305
MboI GATC 7 cut(s) 7, 556, 616, 677, 1217, 1258, 1303
MflI RGATCY 1 cut(s) 1217
MhlI GDGCHC 1 cut(s) 1198
MlsI TGGCCA 1 cut(s) 952
MluCI AATT 4 cut(s) 15, 182, 217, 1413
MluNI TGGCCA 1 cut(s) 952
MlyI GAGTC 2 cut(s) 440, 1153
MmeI TCCRAC 4 cut(s) 50, 989, 1041, 1419
Mox20I TGGCCA 1 cut(s) 952
Mph1103I ATGCAT 1 cut(s) 370
MreI CGCCGGCG 1 cut(s) 203
MroNI GCCGGC 1 cut(s) 203
MscI TGGCCA 1 cut(s) 952
MseI TTAA 5 cut(s) 693, 783, 837, 1037, 1343
Msp20I TGGCCA 1 cut(s) 952
MspI CCGG 3 cut(s) 204, 303, 1347
MspR9I CCNGG 3 cut(s) 172, 1278, 1348
MvaI CCWGG 2 cut(s) 172, 1278
MwoI GCNNNNNNNGC 3 cut(s) 469, 847, 1279
NaeI GCCGGC 1 cut(s) 205
NciI CCSGG 1 cut(s) 1348
NdeII GATC 7 cut(s) 7, 556, 616, 677, 1217, 1258, 1303
NgoMIV GCCGGC 1 cut(s) 203
NlaIII CATG 8 cut(s) 62, 82, 283, 372, 467, 575, 585, 850
NmeAIII GCCGAG 1 cut(s) 512
NmuCI GTSAC 2 cut(s) 292, 466
NsbI TGCGCA 1 cut(s) 462
NsiI ATGCAT 1 cut(s) 370
NspI RCATGY 2 cut(s) 467, 850
NspV TTCGAA 1 cut(s) 476
PaeR7I CTCGAG 2 cut(s) 874, 1197
PceI AGGCCT 1 cut(s) 718
PdiI GCCGGC 1 cut(s) 205
PfeI GAWTC 4 cut(s) 82, 198, 386, 1360
PkrI GCNGC 5 cut(s) 194, 209, 1027, 1229, 1275
PleI GAGTC 2 cut(s) 439, 1153
PpsI GAGTC 2 cut(s) 439, 1153
Ppu21I YACGTR 1 cut(s) 109
PshAI GACNNNNGTC 1 cut(s) 1181
PsiI TTATAA 1 cut(s) 506
Psp124BI GAGCTC 1 cut(s) 1198
Psp6I CCWGG 2 cut(s) 170, 1276
PspFI CCCAGC 1 cut(s) 210
PspGI CCWGG 2 cut(s) 170, 1276
PspXI VCTCGAGB 1 cut(s) 1197
PstI CTGCAG 1 cut(s) 1210
PstNI CAGNNNCTG 1 cut(s) 315
PsuI RGATCY 1 cut(s) 1217
RsaI GTAC 4 cut(s) 231, 329, 607, 1137
RsaNI GTAC 4 cut(s) 230, 328, 606, 1136
SacI GAGCTC 1 cut(s) 1198
SaqAI TTAA 5 cut(s) 693, 783, 837, 1037, 1343
SatI GCNGC 5 cut(s) 193, 208, 1026, 1228, 1274
Sau3AI GATC 7 cut(s) 7, 556, 616, 677, 1217, 1258, 1303
SchI GAGTC 2 cut(s) 440, 1153
ScrFI CCNGG 3 cut(s) 172, 1278, 1348
SduI GDGCHC 1 cut(s) 1198
SfaNI GCATC 4 cut(s) 355, 533, 1247, 1408
SfcI CTRYAG 2 cut(s) 685, 1206
Sfr274I CTCGAG 2 cut(s) 874, 1197
SfuI TTCGAA 1 cut(s) 476
SgrAI CRCCGGYG 1 cut(s) 203
SlaI CTCGAG 2 cut(s) 874, 1197
SmlI CTYRAG 5 cut(s) 851, 874, 1161, 1197, 1457
SmoI CTYRAG 5 cut(s) 851, 874, 1161, 1197, 1457
SpeI ACTAGT 1 cut(s) 925
Sse9I AATT 4 cut(s) 15, 182, 217, 1413
SseBI AGGCCT 1 cut(s) 718
SsiI CCGC 3 cut(s) 193, 207, 1228
SspMI CTAG 5 cut(s) 624, 668, 926, 1179, 1373
SstI GAGCTC 1 cut(s) 1198
StuI AGGCCT 1 cut(s) 718
StyD4I CCNGG 3 cut(s) 170, 1276, 1346
StyI CCWWGG 1 cut(s) 1116
TaaI ACNGT 6 cut(s) 229, 327, 605, 610, 729, 761
TaiI ACGT 5 cut(s) 111, 255, 294, 378, 1061
TaqI TCGA 8 cut(s) 101, 162, 404, 476, 875, 1072, 1198, 1403
TaqII GACCGA 1 cut(s) 1353
TasI AATT 4 cut(s) 15, 182, 217, 1413
TatI WGTACW 2 cut(s) 327, 605
TauI GCSGC 3 cut(s) 195, 210, 1230
TfiI GAWTC 4 cut(s) 82, 198, 386, 1360
Tru1I TTAA 5 cut(s) 693, 783, 837, 1037, 1343
Tru9I TTAA 5 cut(s) 693, 783, 837, 1037, 1343
TscAI CASTG 1 cut(s) 637
TseFI GTSAC 2 cut(s) 292, 466
TseI GCWGC 2 cut(s) 1025, 1273
Tsp45I GTSAC 2 cut(s) 292, 466
TspDTI ATGAA 7 cut(s) 47, 372, 426, 578, 821, 912, 1161
TspGWI ACGGA 1 cut(s) 1372
TspRI CASTG 1 cut(s) 637
XapI RAATTY 1 cut(s) 217
XceI RCATGY 2 cut(s) 467, 850
XhoI CTCGAG 2 cut(s) 874, 1197
XmiI GTMKAC 1 cut(s) 131
XspI CTAG 5 cut(s) 624, 668, 926, 1179, 1373
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.