AT5G32460

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001490
Physical Location & Seq
Reverse (-)
12081803 .. 12084161
2359 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G32460.1

Sequence Viewer

Length: 1593 bp
ATGACTGATGCAATGTTCTCTTCTTCGGCCAAGCCACACTTCATCAAGCCTATGCTTCCAGGCTTCGAAACCTATATTGTTATTCCTAAAGCTTTTTACTCGAACTACTTAGAGGGAAGACAAGAGGGAAACGCTGCAGAACTGAGATCAGACGCCACGGAGATCACTTGGAAAATAAAGATAGACGGCCGAAGAATGACCAAAGGGTGGGAAGAATTTGCGGTGGCTCACAATCTTCAGGTCGACGACATTCTTGTTTTCAGACACGAAGGAAATCTCTTGTTTCATGTCACACCTTTTGGACTGAGTTTTTGTGAGATACTTTACTCACAGAGAGACGAGAAAGATGTCAAAGACACGACGGGAAAGGTTACAAGATCTAGAACGGTGAAGAAAAACGGAAAAAATGAGTGTTCATCAGTGGACACTGATTTTGTGGTCCCTGTTACAGCCTCAAACCAACGAGTTGATTCATTTTATCTTCCAAGAGGTTTTACCACATCAAGTGGTTCGAGTAAACTGTGCAATGAGATAATTCTAATAGATGAAAAAGACAGGCCATCGACGCTAAAACTGCGTTACAATAAATCAAGTAATCGGTTTTGTGTTAGCCGAGGTTGGAGAGCTTTCTGCTGCAGGAATGGTTATAGAACTGGTTGTTTCTTGAGAATCATATTGGTCCGAAAAGGAAAAACACCCGTGCTCCGGATTTTTCCTTTGGAGAGGGATGAGGACAACATTGAAAAACACTCAAAGAAGGTTAAGCAAGAAGTAGAGCATGAGGAGTCTGTGAAAGAAGAGACGAATGTGGAATCAGGAAAGCTTAAAAGAGATAGATTACTGCAGAAAGATCCCAAAAATTTGTGTTCTACATCACAAGACACTAATTTTGTGGTCCCTGTCACAGCTTCAAACCAACGACATGATTCATTTCATCTTCCAAAAGGTTTAACCACCTCAAGTGGTCTGAGTAAACTGTGCAAGAAGATTATTTTTATGGATCAAAAAGGTAGGTCATCGATACTAGACTTGAGCTACAGTATATCAGATGATCGGTTTACTGTCAGGCGAGGCTGGAAAGCTTTCTGCTGCAGGAATGAGCATAAAACTGGTTGTTTCTTGAGGCTCATATTGGTCCAAAATGGAAAAACACCCGTACTCCGGATTTTTCCTTTGGAGAGATATGAGAACAGCATTGCGAAGAACTCTAAGAAGATTAAGCAAGAAGTAGAATATGAGTCTGCGAAAGAAGAGAAGAACCTGGAATCATTGTCTTTGTCAGATAATTCTAGCTTTGTGGTATCTGTCACAGTTTCTAATCTAAGTGAAGATATACTGCATCTTCCAATAAGGCTTTCAAGATCAAACTTACTGGACAAAAAAATTCACGAGATAGTTTTGATGAACAAAGAGGGAAGAACATGGATATTAAGTTTAAAATACAGCAAGTACTCCGGTAGGTTTAGAATCACACGCGGCTGGAAAAGTTTCTGCGAGGCGAACGGTCAAAAACCTGGATGTACCTTCTTACTCAAGCTGCATCTTTCGATGACTCGGTTTGACCAAGACGGAGTAGCATGGGACAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

530

Amino Acids

60.89

Weight (kDa)

9.44

Isoelectric Point (pI)

36.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 25 - 92 3.5e-09 B3 DNA binding domain
B3 PF02362 147 - 239 3e-14 B3 DNA binding domain
B3 PF02362 299 - 391 5.7e-13 B3 DNA binding domain
B3 PF02362 432 - 514 8.3e-16 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 207
AccI GTMKAC 1 cut(s) 243
AccII CGCG 1 cut(s) 1476
AccIII TCCGGA 2 cut(s) 705, 1161
AciI CCGC 2 cut(s) 221, 1476
AclWI GGATC 2 cut(s) 845, 1008
AcoI YGGCCR 2 cut(s) 27, 187
AcsI RAATTY 3 cut(s) 215, 859, 1383
AcuI CTGAAG 1 cut(s) 221
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 3 cut(s) 1158, 1451, 1522
AfiI CCNNNNNNNGG 3 cut(s) 207, 705, 1161
AgsI TTSAA 3 cut(s) 743, 912, 1359
AjnI CCWGG 3 cut(s) 58, 1260, 1513
AjuI GAANNNNNNNTTGG 4 cut(s) 23, 55, 1339, 1371
AluBI AGCT 8 cut(s) 92, 626, 823, 908, 1035, 1082, 1293, 1537
AluI AGCT 8 cut(s) 92, 626, 823, 908, 1035, 1082, 1293, 1537
Alw21I GWGCWC 1 cut(s) 705
Alw26I GTCTC 2 cut(s) 330, 794
AlwI GGATC 2 cut(s) 845, 1008
Aor13HI TCCGGA 2 cut(s) 705, 1161
AoxI GGCC 3 cut(s) 27, 187, 557
ApeKI GCWGC 4 cut(s) 134, 633, 1089, 1537
ApoI RAATTY 3 cut(s) 215, 859, 1383
ArsI GACNNNNNNTTYG 6 cut(s) 294, 326, 416, 448, 872, 904
Asp700I GAANNNNTTC 2 cut(s) 1082, 1487
AspS9I GGNCC 4 cut(s) 439, 679, 895, 1135
AsuHPI GGTGA 1 cut(s) 400
AsuII TTCGAA 1 cut(s) 66
AvaII GGWCC 4 cut(s) 439, 679, 895, 1135
BauI CACGAG 1 cut(s) 1388
BbsI GAAGAC 1 cut(s) 124
Bbv12I GWGCWC 1 cut(s) 705
BbvI GCAGC 4 cut(s) 121, 620, 1076, 1524
BccI CCATC 1 cut(s) 568
BceAI ACGGC 1 cut(s) 202
BciT130I CCWGG 3 cut(s) 60, 1262, 1515
BcoDI GTCTC 2 cut(s) 330, 794
BfaI CTAG 3 cut(s) 381, 1025, 1290
BfmI CTRYAG 5 cut(s) 135, 634, 842, 1036, 1090
BglII AGATCT 1 cut(s) 377
BisI GCNGC 5 cut(s) 135, 634, 1090, 1477, 1538
BlsI GCNGC 5 cut(s) 136, 635, 1091, 1478, 1539
BmcAI AGTACT 1 cut(s) 1451
Bme1390I CCNGG 3 cut(s) 60, 1262, 1515
Bme18I GGWCC 4 cut(s) 439, 679, 895, 1135
BmgT120I GGNCC 4 cut(s) 439, 679, 895, 1135
BmiI GGNNCC 2 cut(s) 441, 897
BmrFI CCNGG 3 cut(s) 60, 1262, 1515
BmsI GCATC 2 cut(s) 1348, 1549
BpiI GAAGAC 1 cut(s) 124
Bpu14I TTCGAA 1 cut(s) 66
BpuEI CTTGAG 5 cut(s) 685, 943, 1051, 1141, 1517
Bsa29I ATCGAT 1 cut(s) 1019
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 2 cut(s) 156, 613
BsaWI WCCGGW 3 cut(s) 705, 1161, 1454
BsaXI ACNNNNNCTCC 4 cut(s) 687, 717, 1143, 1173
Bsc4I CCNNNNNNNGG 3 cut(s) 207, 705, 1161
Bse1I ACTGG 3 cut(s) 658, 1114, 1377
Bse3DI GCAATG 3 cut(s) 18, 532, 1194
BseAI TCCGGA 2 cut(s) 705, 1161
BseBI CCWGG 3 cut(s) 60, 1262, 1515
BseCI ATCGAT 1 cut(s) 1019
BseDI CCNNGG 2 cut(s) 156, 613
BseGI GGATG 2 cut(s) 733, 1523
BseLI CCNNNNNNNGG 3 cut(s) 207, 705, 1161
BseMI GCAATG 3 cut(s) 18, 532, 1194
BseMII CTCAG 3 cut(s) 134, 296, 959
BseNI ACTGG 3 cut(s) 658, 1114, 1377
BseRI GAGGAG 1 cut(s) 797
BseX3I CGGCCG 1 cut(s) 187
BseXI GCAGC 4 cut(s) 121, 620, 1076, 1524
Bsh1236I CGCG 1 cut(s) 1476
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 3 cut(s) 29, 189, 559
BshVI ATCGAT 1 cut(s) 1019
BsiEI CGRYCG 1 cut(s) 190
BsiHKAI GWGCWC 1 cut(s) 705
BsiSI CCGG 3 cut(s) 706, 1162, 1455
BslFI GGGAC 2 cut(s) 425, 881
BslI CCNNNNNNNGG 3 cut(s) 207, 705, 1161
BsmAI GTCTC 2 cut(s) 330, 794
BsmBI CGTCTC 2 cut(s) 330, 794
BsmFI GGGAC 2 cut(s) 425, 881
BsnI GGCC 3 cut(s) 29, 189, 559
Bsp119I TTCGAA 1 cut(s) 66
Bsp1286I GDGCHC 1 cut(s) 705
Bsp13I TCCGGA 2 cut(s) 705, 1161
Bsp143I GATC 7 cut(s) 146, 162, 377, 850, 1000, 1051, 1361
BspACI CCGC 2 cut(s) 221, 1476
BspANI GGCC 3 cut(s) 29, 189, 559
BspCNI CTCAG 3 cut(s) 135, 297, 960
BspDI ATCGAT 1 cut(s) 1019
BspEI TCCGGA 2 cut(s) 705, 1161
BspFNI CGCG 1 cut(s) 1476
BspLI GGNNCC 2 cut(s) 441, 897
BspMAI CTGCAG 4 cut(s) 139, 638, 846, 1094
BspPI GGATC 2 cut(s) 845, 1008
BspT104I TTCGAA 1 cut(s) 66
BsrDI GCAATG 3 cut(s) 18, 532, 1194
BsrI ACTGG 3 cut(s) 658, 1114, 1377
BssECI CCNNGG 2 cut(s) 156, 613
BssMI GATC 7 cut(s) 146, 162, 377, 850, 1000, 1051, 1361
BssNI GRCGYC 1 cut(s) 153
BssSI CACGAG 1 cut(s) 1388
Bst2BI CACGAG 1 cut(s) 1388
Bst2UI CCWGG 3 cut(s) 60, 1262, 1515
Bst4CI ACNGT 7 cut(s) 388, 522, 978, 1040, 1063, 1312, 1505
Bst6I CTCTTC 3 cut(s) 25, 792, 1245
BstACI GRCGYC 1 cut(s) 153
BstBI TTCGAA 1 cut(s) 66
BstDEI CTNAG 6 cut(s) 109, 143, 305, 968, 1209, 1322
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 2 cut(s) 733, 1523
BstFNI CGCG 1 cut(s) 1476
BstKTI GATC 7 cut(s) 149, 165, 380, 853, 1003, 1054, 1364
BstMAI GTCTC 2 cut(s) 330, 794
BstMBI GATC 7 cut(s) 146, 162, 377, 850, 1000, 1051, 1361
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 2 cut(s) 565, 574
BstNI CCWGG 3 cut(s) 60, 1262, 1515
BstSCI CCNGG 3 cut(s) 58, 1260, 1513
BstSFI CTRYAG 5 cut(s) 135, 634, 842, 1036, 1090
BstUI CGCG 1 cut(s) 1476
BstV1I GCAGC 4 cut(s) 121, 620, 1076, 1524
BstV2I GAAGAC 1 cut(s) 124
BstX2I RGATCY 2 cut(s) 377, 850
BstYI RGATCY 2 cut(s) 377, 850
BstZI CGGCCG 1 cut(s) 187
Bsu15I ATCGAT 1 cut(s) 1019
BsuRI GGCC 3 cut(s) 29, 189, 559
BsuTUI ATCGAT 1 cut(s) 1019
BtgI CCRYGG 1 cut(s) 156
BtsCI GGATG 2 cut(s) 733, 1523
BtsIMutI CAGTG 2 cut(s) 426, 426
Cfr13I GGNCC 4 cut(s) 439, 679, 895, 1135
ClaI ATCGAT 1 cut(s) 1019
CseI GACGC 2 cut(s) 161, 574
Csp6I GTAC 3 cut(s) 1157, 1450, 1521
CviAII CATG 5 cut(s) 287, 779, 923, 1422, 1578
CviQI GTAC 3 cut(s) 1157, 1450, 1521
DdeI CTNAG 6 cut(s) 109, 143, 305, 968, 1209, 1322
DpnI GATC 7 cut(s) 148, 164, 379, 852, 1002, 1053, 1363
DpnII GATC 7 cut(s) 146, 162, 377, 850, 1000, 1051, 1361
DraI TTTAAA 1 cut(s) 1437
EaeI YGGCCR 2 cut(s) 27, 187
EagI CGGCCG 1 cut(s) 187
Eam1104I CTCTTC 3 cut(s) 25, 792, 1245
EarI CTCTTC 3 cut(s) 25, 792, 1245
EclXI CGGCCG 1 cut(s) 187
Eco47I GGWCC 4 cut(s) 439, 679, 895, 1135
Eco52I CGGCCG 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 221
EcoRII CCWGG 3 cut(s) 58, 1260, 1513
Esp3I CGTCTC 2 cut(s) 330, 794
FaeI CATG 5 cut(s) 290, 782, 926, 1425, 1581
FalI AAGNNNNNCTT 2 cut(s) 23, 55
FaqI GGGAC 2 cut(s) 425, 881
FatI CATG 5 cut(s) 286, 778, 922, 1421, 1577
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 5 cut(s) 135, 634, 1090, 1477, 1538
FokI GGATG 2 cut(s) 740, 1530
Fsp4HI GCNGC 5 cut(s) 135, 634, 1090, 1477, 1538
FspBI CTAG 3 cut(s) 381, 1025, 1290
GluI GCNGC 5 cut(s) 135, 634, 1090, 1477, 1538
HaeIII GGCC 3 cut(s) 29, 189, 559
HapII CCGG 3 cut(s) 706, 1162, 1455
HgaI GACGC 2 cut(s) 161, 574
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 5 cut(s) 290, 782, 926, 1425, 1581
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HindIII AAGCTT 3 cut(s) 90, 821, 1080
HinfI GANTC 9 cut(s) 470, 669, 785, 812, 926, 1238, 1265, 1467, 1552
HpaII CCGG 3 cut(s) 706, 1162, 1455
HphI GGTGA 1 cut(s) 400
Hpy166II GTNNAC 5 cut(s) 244, 424, 518, 974, 1059
Hpy188I TCNGA 6 cut(s) 151, 263, 683, 969, 1048, 1282
Hpy188III TCNNGA 8 cut(s) 381, 664, 706, 816, 1120, 1162, 1359, 1388
Hpy8I GTNNAC 5 cut(s) 244, 424, 518, 974, 1059
Hpy99I CGWCG 3 cut(s) 248, 364, 568
HpyAV CCTTC 3 cut(s) 263, 751, 1534
HpyCH4III ACNGT 7 cut(s) 388, 522, 978, 1040, 1063, 1312, 1505
HpyCH4V TGCA 9 cut(s) 11, 137, 525, 636, 844, 981, 1092, 1339, 1540
HpyF10VI GCNNNNNNNGC 2 cut(s) 565, 574
HpyF3I CTNAG 6 cut(s) 109, 143, 305, 968, 1209, 1322
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 5 cut(s) 290, 782, 926, 1425, 1581
Kpn2I TCCGGA 2 cut(s) 705, 1161
Kzo9I GATC 7 cut(s) 146, 162, 377, 850, 1000, 1051, 1361
LmnI GCTCC 1 cut(s) 708
Lsp1109I GCAGC 4 cut(s) 121, 620, 1076, 1524
LweI GCATC 2 cut(s) 1348, 1549
MaeI CTAG 3 cut(s) 381, 1025, 1290
MaeIII GTNAC 6 cut(s) 289, 370, 445, 578, 901, 1306
MalI GATC 7 cut(s) 148, 164, 379, 852, 1002, 1053, 1363
MboI GATC 7 cut(s) 146, 162, 377, 850, 1000, 1051, 1361
MflI RGATCY 2 cut(s) 377, 850
MhlI GDGCHC 1 cut(s) 705
MluCI AATT 6 cut(s) 215, 534, 859, 886, 1285, 1383
MlyI GAGTC 3 cut(s) 794, 1247, 1546
MmeI TCCRAC 1 cut(s) 599
MroI TCCGGA 2 cut(s) 705, 1161
MroXI GAANNNNTTC 2 cut(s) 1082, 1487
MseI TTAA 6 cut(s) 762, 825, 950, 1218, 1430, 1436
MspI CCGG 3 cut(s) 706, 1162, 1455
MspR9I CCNGG 3 cut(s) 60, 1262, 1515
MvaI CCWGG 3 cut(s) 60, 1262, 1515
MvnI CGCG 1 cut(s) 1476
MwoI GCNNNNNNNGC 2 cut(s) 565, 574
NdeII GATC 7 cut(s) 146, 162, 377, 850, 1000, 1051, 1361
NlaIII CATG 5 cut(s) 290, 782, 926, 1425, 1581
NlaIV GGNNCC 2 cut(s) 441, 897
NmeAIII GCCGAG 1 cut(s) 638
NmuCI GTSAC 3 cut(s) 289, 901, 1306
NspV TTCGAA 1 cut(s) 66
PdmI GAANNNNTTC 2 cut(s) 1082, 1487
PfeI GAWTC 6 cut(s) 470, 669, 812, 926, 1265, 1467
PflMI CCANNNNNTGG 1 cut(s) 207
PkrI GCNGC 5 cut(s) 136, 635, 1091, 1478, 1539
PleI GAGTC 3 cut(s) 793, 1246, 1546
PpsI GAGTC 3 cut(s) 793, 1246, 1546
Psp6I CCWGG 3 cut(s) 58, 1260, 1513
PspGI CCWGG 3 cut(s) 58, 1260, 1513
PspN4I GGNNCC 2 cut(s) 441, 897
PspPI GGNCC 4 cut(s) 439, 679, 895, 1135
PstI CTGCAG 4 cut(s) 139, 638, 846, 1094
PsuI RGATCY 2 cut(s) 377, 850
RsaI GTAC 3 cut(s) 1158, 1451, 1522
RsaNI GTAC 3 cut(s) 1157, 1450, 1521
SalI GTCGAC 1 cut(s) 242
SaqAI TTAA 6 cut(s) 762, 825, 950, 1218, 1430, 1436
SatI GCNGC 5 cut(s) 135, 634, 1090, 1477, 1538
Sau3AI GATC 7 cut(s) 146, 162, 377, 850, 1000, 1051, 1361
Sau96I GGNCC 4 cut(s) 439, 679, 895, 1135
ScaI AGTACT 1 cut(s) 1451
SchI GAGTC 3 cut(s) 794, 1247, 1546
ScrFI CCNGG 3 cut(s) 60, 1262, 1515
SduI GDGCHC 1 cut(s) 705
SfaNI GCATC 2 cut(s) 1348, 1549
SfcI CTRYAG 5 cut(s) 135, 634, 842, 1036, 1090
SfuI TTCGAA 1 cut(s) 66
SinI GGWCC 4 cut(s) 439, 679, 895, 1135
SmlI CTYRAG 5 cut(s) 664, 958, 1030, 1120, 1532
SmoI CTYRAG 5 cut(s) 664, 958, 1030, 1120, 1532
Sse9I AATT 6 cut(s) 215, 534, 859, 886, 1285, 1383
SsiI CCGC 2 cut(s) 221, 1476
SspMI CTAG 3 cut(s) 381, 1025, 1290
StyD4I CCNGG 3 cut(s) 58, 1260, 1513
TaaI ACNGT 7 cut(s) 388, 522, 978, 1040, 1063, 1312, 1505
TaqI TCGA 7 cut(s) 66, 101, 243, 512, 563, 1019, 1547
TasI AATT 6 cut(s) 215, 534, 859, 886, 1285, 1383
TatI WGTACW 1 cut(s) 1449
TauI GCSGC 1 cut(s) 1479
TfiI GAWTC 6 cut(s) 470, 669, 812, 926, 1265, 1467
Tru1I TTAA 6 cut(s) 762, 825, 950, 1218, 1430, 1436
Tru9I TTAA 6 cut(s) 762, 825, 950, 1218, 1430, 1436
TscAI CASTG 2 cut(s) 426, 433
TseFI GTSAC 3 cut(s) 289, 901, 1306
TseI GCWGC 4 cut(s) 134, 633, 1089, 1537
Tsp45I GTSAC 3 cut(s) 289, 901, 1306
TspDTI ATGAA 8 cut(s) 31, 275, 405, 462, 561, 918, 923, 1418
TspGWI ACGGA 3 cut(s) 173, 414, 1584
TspRI CASTG 2 cut(s) 426, 433
Van91I CCANNNNNTGG 1 cut(s) 207
VpaK11BI GGWCC 4 cut(s) 439, 679, 895, 1135
XapI RAATTY 3 cut(s) 215, 859, 1383
XbaI TCTAGA 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 243
XmnI GAANNNNTTC 2 cut(s) 1082, 1487
XspI CTAG 3 cut(s) 381, 1025, 1290
ZrmI AGTACT 1 cut(s) 1451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.