AT4G31630

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001490
Physical Location & Seq
Reverse (-)
15325343 .. 15327220
1878 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31630.1

Sequence Viewer

Length: 1539 bp
ATGGCGGATCCACTGATTCCCTCCCCAACCAACAAAGCTTTCTTCATCATAGATCTATCTGGACAAAAATCCAATCCTATCATTCCTACTGAGTTCATTTGGAATCACTTCAATGGTAAGATTCAGTCAACGAATATGAAACTAACTTCTGACGCTTCGGACAGAAACTGGGACGTGAAATTGGACGGCGCGAGATTCGCCGGCGGATGGAAAGATTTCTCCGTCTCCCACTCTGTTAGAGACGACGACCTTTTGAGTTTCCGGCACGACGGAGGCATGGTCTTCCACGTCTCACCCTTCGGACGTAGTTTCTCTCAGATACAGTTAATCTCTTCTTCTACCTCTGACGATGATGATGATGAACGCACTGTTTTTGATGATGATGAAGACGACGATGTAGGAGATGATGATGACAATTCGATATCCGAAGATGATTTTTGTTCAAAGAAAATTTCGTCGAAGAAGAGAGCAAGAAAAGAAACAGAGTCTTCATCAGACAAATCTTATCTTGTTGCACACGTCACACCTTCAAGCCTACTCCGAGATAATATGTGTGTTTTAAGCAAATTTGCAAGGTCAAATGGTCTGGACAGGAGAGAGTGTGAGATTGATCTGAGGGATGAACATGAAAAATCTTGGACTCTGCTTTTGAGACACAACAAAAAAACTGGTCAGGCTTTTATGCGTGGAGGATGGAGAAGTTTTTGTCGTAATAACGGGATCAAAGCTGGATCTATCTGTAGGTTTAAGCTTGTCCAAAGCGGAATAAAACCTGTGCTACAGCTGTGTCCCAACGCCTCTAGCATTCCAGAAGGAAACTCTTCCAAAGCAAGAAAAAAACGAAATGTTTCTGAAATTGAAGGTGATGAGATAGAATCTGAGAATTGCTCAGAGACTATTCCATTGAATCAGAACAAGATTCTGACGTTTGATCTTAAACCTTACGTGTTCAGGTCATGTCAATTTTTTCTTCCTGCATCGTTCGCAAGAGAGAACGGGATCGTGGAAGCAGGAGAGGTAACTGTATTGAACAAAGATGGCATAGAGTGGAAGTCGCATCTAGTTAACATCAAGGGACGCGATCAGTTTTACAATAGAGGTTGTCAAGATTTCTTTGTAGCTAATGGCGTAAAGAATGTTGGTGATCCCTTCACATTGGAGGTTATTCGAGGAGGACCATCTCCAATTCTCAAGATCTGTTCCAAGGTGAAACAAGCAGCATCATCTGATGGCCATAAGACTGCGGATAGGAAACCCCGAATGACGGATCAAGCACCACTGGCTGAAGAACAAACTGATAATCGGGTCGAAAAGAGAGCTCAAGTTACTGAGGAAGGAGGGCCATCTCGCAGCACTAGGGCTGATCCGGGAAACTTGCAGCAAAAGCAACCTTGCTCAATATCTGATCACGTTAAAAAGGTGAAACAGAGTATTGTGGATACTTTAACCGATGTAAGGCGGTTTCAGTCGGAGCTCAAGGTAAAAGAACAGAATCTAGAAGCTTCATTGCAAGAAATTGATGCGTTAGGTATGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

512

Amino Acids

57.35

Weight (kDa)

6.02

Isoelectric Point (pI)

45.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 24 - 97 1e-05 B3 DNA binding domain
B3 PF02362 172 - 265 1.2e-23 B3 DNA binding domain
B3 PF02362 311 - 402 8.9e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 191, 1080
AciI CCGC 5 cut(s) 5, 204, 762, 1244, 1459
AclWI GGATC 8 cut(s) 2, 15, 728, 739, 1007, 1139, 1275, 1358
AcoI YGGCCR 1 cut(s) 1231
AcsI RAATTY 2 cut(s) 450, 566
AcuI CTGAAG 1 cut(s) 1305
AfiI CCNNNNNNNGG 3 cut(s) 207, 1264, 1455
AflIII ACRYGT 2 cut(s) 517, 945
AgsI TTSAA 6 cut(s) 112, 444, 531, 860, 907, 1030
AjiI CACGTC 3 cut(s) 175, 289, 520
AjuI GAANNNNNNNTTGG 2 cut(s) 23, 55
AluBI AGCT 8 cut(s) 38, 728, 751, 784, 1121, 1319, 1474, 1502
AluI AGCT 8 cut(s) 38, 728, 751, 784, 1121, 1319, 1474, 1502
Alw21I GWGCWC 2 cut(s) 1321, 1476
Alw26I GTCTC 5 cut(s) 229, 234, 295, 646, 887
AlwI GGATC 8 cut(s) 2, 15, 728, 739, 1007, 1139, 1275, 1358
AlwNI CAGNNNCTG 1 cut(s) 168
AoxI GGCC 2 cut(s) 1231, 1340
ApeKI GCWGC 3 cut(s) 1217, 1350, 1378
ApoI RAATTY 2 cut(s) 450, 566
ArsI GACNNNNNNTTYG 2 cut(s) 631, 663
Asp700I GAANNNNTTC 4 cut(s) 107, 215, 820, 847
AspLEI GCGC 1 cut(s) 191
AspS9I GGNCC 2 cut(s) 1175, 1340
AsuC2I CCSGG 1 cut(s) 1368
AsuHPI GGTGA 5 cut(s) 285, 875, 1154, 1219, 1432
AvaII GGWCC 1 cut(s) 1175
BalI TGGCCA 1 cut(s) 1233
BamHI GGATCC 1 cut(s) 7
BanII GRGCYC 2 cut(s) 1321, 1476
BbsI GAAGAC 3 cut(s) 274, 393, 480
Bbv12I GWGCWC 2 cut(s) 1321, 1476
BbvI GCAGC 3 cut(s) 1229, 1362, 1390
BccI CCATC 6 cut(s) 201, 687, 1031, 1186, 1223, 1351
BceAI ACGGC 1 cut(s) 202
BciVI GTATCC 1 cut(s) 1432
BclI TGATCA 1 cut(s) 1405
BcnI CCSGG 1 cut(s) 1368
BcoDI GTCTC 5 cut(s) 229, 234, 295, 646, 887
BfaI CTAG 4 cut(s) 801, 1061, 1356, 1496
BfmI CTRYAG 2 cut(s) 739, 779
BfuI GTATCC 1 cut(s) 1432
BglII AGATCT 2 cut(s) 52, 1194
BisI GCNGC 3 cut(s) 1218, 1351, 1379
BlsI GCNGC 3 cut(s) 1219, 1352, 1380
Bme1390I CCNGG 1 cut(s) 1368
Bme18I GGWCC 1 cut(s) 1175
BmgBI CACGTC 3 cut(s) 175, 289, 520
BmgT120I GGNCC 2 cut(s) 1175, 1340
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 1 cut(s) 1368
BmrI ACTGGG 1 cut(s) 178
BmsI GCATC 4 cut(s) 986, 1066, 1229, 1510
BmuI ACTGGG 1 cut(s) 178
BpiI GAAGAC 3 cut(s) 274, 393, 480
BplI GAGNNNNNCTC 2 cut(s) 872, 904
BpuEI CTTGAG 3 cut(s) 1175, 1305, 1460
BpuMI CCSGG 1 cut(s) 1368
BsaAI YACGTR 1 cut(s) 946
BsaJI CCNNGG 1 cut(s) 1203
BsaXI ACNNNNNCTCC 4 cut(s) 264, 294, 586, 616
Bsc4I CCNNNNNNNGG 3 cut(s) 207, 1264, 1455
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 3 cut(s) 173, 673, 1284
Bse3DI GCAATG 1 cut(s) 1505
BseDI CCNNGG 1 cut(s) 1203
BseGI GGATG 3 cut(s) 212, 625, 698
BseLI CCNNNNNNNGG 3 cut(s) 207, 1264, 1455
BseMI GCAATG 1 cut(s) 1505
BseMII CTCAG 6 cut(s) 81, 329, 605, 870, 903, 1320
BseNI ACTGG 3 cut(s) 173, 673, 1284
BseRI GAGGAG 1 cut(s) 1185
BseXI GCAGC 3 cut(s) 1229, 1362, 1390
Bsh1236I CGCG 2 cut(s) 191, 1080
BshFI GGCC 2 cut(s) 1233, 1342
BsiHKAI GWGCWC 2 cut(s) 1321, 1476
BsiSI CCGG 3 cut(s) 201, 262, 1367
BslFI GGGAC 3 cut(s) 185, 774, 1089
BslI CCNNNNNNNGG 3 cut(s) 207, 1264, 1455
BsmAI GTCTC 5 cut(s) 229, 234, 295, 646, 887
BsmBI CGTCTC 3 cut(s) 229, 234, 295
BsmFI GGGAC 3 cut(s) 185, 774, 1089
BsmI GAATGC 1 cut(s) 804
BsnI GGCC 2 cut(s) 1233, 1342
Bsp1286I GDGCHC 2 cut(s) 1321, 1476
BspACI CCGC 5 cut(s) 5, 204, 762, 1244, 1459
BspANI GGCC 2 cut(s) 1233, 1342
BspCNI CTCAG 6 cut(s) 82, 328, 606, 871, 902, 1321
BspFNI CGCG 2 cut(s) 191, 1080
BspLI GGNNCC 1 cut(s) 9
BspPI GGATC 8 cut(s) 2, 15, 728, 739, 1007, 1139, 1275, 1358
BsrDI GCAATG 1 cut(s) 1505
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 3 cut(s) 173, 673, 1284
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 1 cut(s) 1203
BssT1I CCWWGG 1 cut(s) 1203
Bst4CI ACNGT 3 cut(s) 324, 370, 1024
Bst6I CTCTTC 3 cut(s) 337, 458, 826
BstBAI YACGTR 1 cut(s) 946
BstC8I GCNNGC 1 cut(s) 202
BstDEI CTNAG 6 cut(s) 90, 315, 614, 879, 889, 1329
BstF5I GGATG 3 cut(s) 212, 625, 698
BstFNI CGCG 2 cut(s) 191, 1080
BstHHI GCGC 1 cut(s) 191
BstMAI GTCTC 5 cut(s) 229, 234, 295, 646, 887
BstMWI GCNNNNNNNGC 4 cut(s) 197, 983, 1280, 1384
BstSCI CCNGG 1 cut(s) 1366
BstSFI CTRYAG 2 cut(s) 739, 779
BstUI CGCG 2 cut(s) 191, 1080
BstV1I GCAGC 3 cut(s) 1229, 1362, 1390
BstV2I GAAGAC 3 cut(s) 274, 393, 480
BstX2I RGATCY 4 cut(s) 7, 52, 731, 1194
BstYI RGATCY 4 cut(s) 7, 52, 731, 1194
BsuI GTATCC 1 cut(s) 1432
BsuRI GGCC 2 cut(s) 1233, 1342
BtrI CACGTC 3 cut(s) 175, 289, 520
BtsCI GGATG 3 cut(s) 212, 625, 698
BtsIMutI CAGTG 3 cut(s) 11, 366, 1277
Cac8I GCNNGC 1 cut(s) 202
CaiI CAGNNNCTG 1 cut(s) 168
CfoI GCGC 1 cut(s) 191
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 2 cut(s) 1175, 1340
CseI GACGC 2 cut(s) 161, 1086
CviAII CATG 3 cut(s) 277, 626, 957
DdeI CTNAG 6 cut(s) 90, 315, 614, 879, 889, 1329
EaeI YGGCCR 1 cut(s) 1231
Eam1104I CTCTTC 3 cut(s) 337, 458, 826
EarI CTCTTC 3 cut(s) 337, 458, 826
EciI GGCGGA 2 cut(s) 20, 219
Ecl136II GAGCTC 2 cut(s) 1319, 1474
Eco130I CCWWGG 1 cut(s) 1203
Eco24I GRGCYC 2 cut(s) 1321, 1476
Eco32I GATATC 1 cut(s) 423
Eco47I GGWCC 1 cut(s) 1175
Eco53kI GAGCTC 2 cut(s) 1319, 1474
Eco57I CTGAAG 1 cut(s) 1305
EcoICRI GAGCTC 2 cut(s) 1319, 1474
EcoRV GATATC 1 cut(s) 423
EcoT14I CCWWGG 1 cut(s) 1203
EcoT38I GRGCYC 2 cut(s) 1321, 1476
ErhI CCWWGG 1 cut(s) 1203
Esp3I CGTCTC 3 cut(s) 229, 234, 295
FaeI CATG 3 cut(s) 280, 629, 960
FaqI GGGAC 3 cut(s) 185, 774, 1089
FatI CATG 3 cut(s) 276, 625, 956
FbaI TGATCA 1 cut(s) 1405
Fnu4HI GCNGC 3 cut(s) 1218, 1351, 1379
FokI GGATG 3 cut(s) 219, 632, 705
FriOI GRGCYC 2 cut(s) 1321, 1476
Fsp4HI GCNGC 3 cut(s) 1218, 1351, 1379
FspBI CTAG 4 cut(s) 801, 1061, 1356, 1496
GlaI GCGC 1 cut(s) 190
GluI GCNGC 3 cut(s) 1218, 1351, 1379
HaeIII GGCC 2 cut(s) 1233, 1342
HapII CCGG 3 cut(s) 201, 262, 1367
HgaI GACGC 2 cut(s) 161, 1086
HhaI GCGC 1 cut(s) 191
Hin1II CATG 3 cut(s) 280, 629, 960
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HincII GTYRAC 2 cut(s) 129, 1066
HindII GTYRAC 2 cut(s) 129, 1066
HindIII AAGCTT 3 cut(s) 36, 749, 1500
HpaI GTTAAC 1 cut(s) 1066
HpaII CCGG 3 cut(s) 201, 262, 1367
HphI GGTGA 5 cut(s) 285, 875, 1154, 1219, 1432
Hpy166II GTNNAC 2 cut(s) 129, 1066
Hpy188III TCNNGA 6 cut(s) 60, 587, 809, 1106, 1192, 1496
Hpy8I GTNNAC 2 cut(s) 129, 1066
Hpy99I CGWCG 4 cut(s) 248, 272, 395, 460
HpyAV CCTTC 6 cut(s) 307, 537, 806, 854, 1159, 1328
HpyCH4III ACNGT 3 cut(s) 324, 370, 1024
HpyCH4IV ACGT 7 cut(s) 174, 288, 304, 519, 926, 945, 1410
HpyCH4V TGCA 5 cut(s) 515, 572, 977, 1378, 1510
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 983, 1280, 1384
HpyF3I CTNAG 6 cut(s) 90, 315, 614, 879, 889, 1329
HpySE526I ACGT 7 cut(s) 174, 288, 304, 519, 926, 945, 1410
Hsp92II CATG 3 cut(s) 280, 629, 960
HspAI GCGC 1 cut(s) 189
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Ksp22I TGATCA 1 cut(s) 1405
KspAI GTTAAC 1 cut(s) 1066
LmnI GCTCC 1 cut(s) 1471
Lsp1109I GCAGC 3 cut(s) 1229, 1362, 1390
LweI GCATC 4 cut(s) 986, 1066, 1229, 1510
MaeI CTAG 4 cut(s) 801, 1061, 1356, 1496
MaeII ACGT 7 cut(s) 174, 288, 304, 519, 926, 945, 1410
MaeIII GTNAC 3 cut(s) 520, 1018, 1324
MflI RGATCY 4 cut(s) 7, 52, 731, 1194
MhlI GDGCHC 2 cut(s) 1321, 1476
MlsI TGGCCA 1 cut(s) 1233
MluCI AATT 9 cut(s) 179, 415, 450, 566, 855, 883, 962, 1185, 1515
MluNI TGGCCA 1 cut(s) 1233
MlyI GAGTC 2 cut(s) 494, 634
MmeI TCCRAC 1 cut(s) 1449
Mox20I TGGCCA 1 cut(s) 1233
MreI CGCCGGCG 1 cut(s) 200
MroNI GCCGGC 1 cut(s) 200
MroXI GAANNNNTTC 4 cut(s) 107, 215, 820, 847
MscI TGGCCA 1 cut(s) 1233
MseI TTAA 7 cut(s) 326, 560, 747, 936, 1065, 1413, 1445
MslI CAYNNNNRTG 1 cut(s) 111
Msp20I TGGCCA 1 cut(s) 1233
MspA1I CMGCKG 1 cut(s) 784
MspI CCGG 3 cut(s) 201, 262, 1367
MspR9I CCNGG 1 cut(s) 1368
Mva1269I GAATGC 1 cut(s) 804
MvnI CGCG 2 cut(s) 191, 1080
MwoI GCNNNNNNNGC 4 cut(s) 197, 983, 1280, 1384
NaeI GCCGGC 1 cut(s) 202
NciI CCSGG 1 cut(s) 1368
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 3 cut(s) 280, 629, 960
NlaIV GGNNCC 1 cut(s) 9
NmuCI GTSAC 1 cut(s) 520
PctI GAATGC 1 cut(s) 804
PdiI GCCGGC 1 cut(s) 202
PdmI GAANNNNTTC 4 cut(s) 107, 215, 820, 847
PfeI GAWTC 8 cut(s) 16, 103, 121, 195, 875, 907, 919, 1492
PfoI TCCNGGA 1 cut(s) 1366
PkrI GCNGC 3 cut(s) 1219, 1352, 1380
PleI GAGTC 2 cut(s) 493, 634
PpsI GAGTC 2 cut(s) 493, 634
Ppu21I YACGTR 1 cut(s) 946
Psp124BI GAGCTC 2 cut(s) 1321, 1476
PspN4I GGNNCC 1 cut(s) 9
PspPI GGNCC 2 cut(s) 1175, 1340
PstNI CAGNNNCTG 1 cut(s) 168
PsuI RGATCY 4 cut(s) 7, 52, 731, 1194
PvuII CAGCTG 1 cut(s) 784
RseI CAYNNNNRTG 1 cut(s) 111
SacI GAGCTC 2 cut(s) 1321, 1476
SaqAI TTAA 7 cut(s) 326, 560, 747, 936, 1065, 1413, 1445
SatI GCNGC 3 cut(s) 1218, 1351, 1379
Sau96I GGNCC 2 cut(s) 1175, 1340
SchI GAGTC 2 cut(s) 494, 634
ScrFI CCNGG 1 cut(s) 1368
SduI GDGCHC 2 cut(s) 1321, 1476
SfaNI GCATC 4 cut(s) 986, 1066, 1229, 1510
SfcI CTRYAG 2 cut(s) 739, 779
SgrAI CRCCGGYG 1 cut(s) 200
SinI GGWCC 1 cut(s) 1175
SmiMI CAYNNNNRTG 1 cut(s) 111
SmlI CTYRAG 3 cut(s) 1190, 1320, 1475
SmoI CTYRAG 3 cut(s) 1190, 1320, 1475
Sse9I AATT 9 cut(s) 179, 415, 450, 566, 855, 883, 962, 1185, 1515
SsiI CCGC 5 cut(s) 5, 204, 762, 1244, 1459
SspMI CTAG 4 cut(s) 801, 1061, 1356, 1496
SstI GAGCTC 2 cut(s) 1321, 1476
StyD4I CCNGG 1 cut(s) 1366
StyI CCWWGG 1 cut(s) 1203
TaaI ACNGT 3 cut(s) 324, 370, 1024
TaiI ACGT 7 cut(s) 177, 291, 307, 522, 929, 948, 1413
TaqI TCGA 4 cut(s) 419, 458, 1168, 1308
TasI AATT 9 cut(s) 179, 415, 450, 566, 855, 883, 962, 1185, 1515
TfiI GAWTC 8 cut(s) 16, 103, 121, 195, 875, 907, 919, 1492
Tru1I TTAA 7 cut(s) 326, 560, 747, 936, 1065, 1413, 1445
Tru9I TTAA 7 cut(s) 326, 560, 747, 936, 1065, 1413, 1445
TscAI CASTG 3 cut(s) 18, 373, 1284
TseFI GTSAC 1 cut(s) 520
TseI GCWGC 3 cut(s) 1217, 1350, 1378
Tsp45I GTSAC 1 cut(s) 520
TspDTI ATGAA 9 cut(s) 34, 85, 152, 375, 399, 480, 636, 642, 1494
TspGWI ACGGA 3 cut(s) 211, 285, 1280
TspRI CASTG 3 cut(s) 18, 373, 1284
VpaK11BI GGWCC 1 cut(s) 1175
XapI RAATTY 2 cut(s) 450, 566
XbaI TCTAGA 1 cut(s) 1495
XmnI GAANNNNTTC 4 cut(s) 107, 215, 820, 847
XspI CTAG 4 cut(s) 801, 1061, 1356, 1496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.