AT4G00260

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001490
Physical Location & Seq
Forward (+)
114971 .. 117051
2081 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G00260.1

Sequence Viewer

Length: 1587 bp
ATGGCGCATCAACATTTCTTCAAGCCTCTTCTTCCTGGCTTCCACGCCTCCTTGACAATTCCTGTAGCCTTCTTCTTGAAGTATATAGAAGGAAGATATGAGCAGAAGACGGCGAAGCTGAGATCAGACGCGTCAAAGAGAACTTGGGAAGTGAAGATAGATGGCCAGAGACTCACCGACGGTTGGAAAGAGTTTGCTGTCTCACATGATCTTCGAATCGGTGACATTGTTGTTTTCAGACAAGAGAGTGACTTGGCTTTCCATGTAACACTGTTGGGACCTAGTTGTTGTGGGATTCAATATGGTTCGTGTTCAGTCGAAAAGAACAACCTCGGTGACGAGAAAAAGAAAGTGAAGGAGAATCCAAATGGAGAAGCAGAGTCTTCTTCACGAGATCCCTCTTGTTTTGTGGCTAATGTTGCGCCTTCGAGTCTACGTTATGACTTGATGAGATTTCCAAGGGGTTTTGTGAGGGATAATGGTGTAGTCGGATCTGGAGAGATTGTTCTGATGAATGAAAAGGGCAGATCATGGAATTTTAACTTGAGACAAAAGCCATCAAACGGAACAGTTTATGTTAGAGGAGGGTGGGTGAGTTTTTGTGATGCCAATGGGCTTAAAGCTGGAGATAACTACACTTTCAAACTGATCAAAAGAGCAGGAACTCTTGTTCTACGTTTGTTACCCAATGAGCCAAAAGAGGAAGCTAATGAAGTGTCTCTTCCCGAAGAACCGGAAAGCGATGCAGAGCGCAACCTTGAAAAGATTCAAAGGAAGGAGAAAGTGAAGAAGAATGTAACAAGAGAGGCAGAGTCTTCTTCACAAGATCCCTCTTGTTTTGTGGCTAATGTCTCCCCTTCGAGTCTACGCTATGACACACTGTATCTTCCAAAGCGTTTTATGAGGGAAAATGGTGTAGACAAAAGATGTGGAGAGATGATTCTGATTAATGAAAAGGGAAAATCATGGACTTTAGATTTGAAAGTAAAGAAATCATCCGGAACTTCTCTCATCAAACGAGGATGGAGAAGTTTCTGTAGTGCCAATGGACTAAGAGCTGGAAGTATCATAACTCTCAAACTGATAAAGAAAAGAGCAACTCTTGTTCTACGTTTGATCCCCAACGAGCCAGAAGAAGCTAATGAAGTAGTCTCTCTTTCGACAGAGCAAGAAAGCGATGAAGAGAGTATCCACGACGAGAAAATCTCAAGAAGAAAGTCTTTACTATCCGAAAACCGATTTGTGACATTAACTCTAACACCTTATACAATCCAAAGTTCTCTACTGAATGAGAATCTTTTGTGTGAATCTATGTTTCAGCGTCTTCCGGTTCCTTTCACGAGGATGAATGGTATCAATGAAGAAACTAAAATGACTCTGTTGGATAAACATGGTGTGAAGTGGTTAACGACTCTGCGGTTCGAGGACGACAAAAGAAAAAGACTACGAATGGTAGGAGGATGGCAAGGATTCATCCAAGCTAACGATGTGAAGGCAAACGAATCCATCATGTTGGAACTGATTTGGGAAGAAGAAACAAGTTGCGTCCTTAAGTTCTGCTCCAAGGTGAAGCTAGAAATCAAATGA

Protein Analysis

528

Amino Acids

60.03

Weight (kDa)

9.03

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 15 - 90 1.3e-09 B3 DNA binding domain
B3 PF02362 136 - 229 2.3e-23 B3 DNA binding domain
B3 PF02362 280 - 374 2.3e-23 B3 DNA binding domain
B3 PF02362 440 - 522 6.5e-17 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 433, 865, 918
AccII CGCG 1 cut(s) 131
AccIII TCCGGA 1 cut(s) 998
AciI CCGC 1 cut(s) 1417
AclWI GGATC 4 cut(s) 389, 499, 821, 1111
AcoI YGGCCR 1 cut(s) 163
AcsI RAATTY 1 cut(s) 535
AfiI CCNNNNNNNGG 2 cut(s) 183, 563
AflII CTTAAG 1 cut(s) 1550
AflIII ACRYGT 1 cut(s) 129
AgsI TTSAA 7 cut(s) 22, 79, 299, 643, 761, 770, 982
AjnI CCWGG 1 cut(s) 34
AloI GAACNNNNNNTCC 4 cut(s) 489, 521, 654, 686
AluBI AGCT 7 cut(s) 118, 623, 707, 1058, 1139, 1481, 1573
AluI AGCT 7 cut(s) 118, 623, 707, 1058, 1139, 1481, 1573
Alw26I GTCTC 6 cut(s) 163, 205, 541, 723, 856, 1156
AlwI GGATC 4 cut(s) 389, 499, 821, 1111
Aor13HI TCCGGA 1 cut(s) 998
AoxI GGCC 1 cut(s) 163
ApoI RAATTY 1 cut(s) 535
ArsI GACNNNNNNTTYG 2 cut(s) 961, 993
AseI ATTAAT 1 cut(s) 948
Asp700I GAANNNNTTC 1 cut(s) 765
AspLEI GCGC 3 cut(s) 7, 424, 753
AspS9I GGNCC 1 cut(s) 278
AsuHPI GGTGA 5 cut(s) 166, 233, 347, 604, 1579
AsuII TTCGAA 1 cut(s) 214
AvaII GGWCC 1 cut(s) 278
BalI TGGCCA 1 cut(s) 165
BauI CACGAG 2 cut(s) 390, 1339
BbsI GAAGAC 4 cut(s) 113, 375, 807, 1316
BccI CCATC 5 cut(s) 155, 565, 1017, 1455, 1514
BceAI ACGGC 1 cut(s) 126
BciT130I CCWGG 1 cut(s) 36
BciVI GTATCC 1 cut(s) 1199
BclI TGATCA 1 cut(s) 648
BcoDI GTCTC 6 cut(s) 163, 205, 541, 723, 856, 1156
BfaI CTAG 2 cut(s) 282, 1574
BfmI CTRYAG 2 cut(s) 63, 1036
BfrI CTTAAG 1 cut(s) 1550
BfuI GTATCC 1 cut(s) 1199
Bme1390I CCNGG 1 cut(s) 36
Bme18I GGWCC 1 cut(s) 278
BmgT120I GGNCC 1 cut(s) 278
BmiI GGNNCC 2 cut(s) 279, 1332
BmrFI CCNGG 1 cut(s) 36
BmsI GCATC 3 cut(s) 16, 595, 733
BpiI GAAGAC 4 cut(s) 113, 375, 807, 1316
BplI GAGNNNNNCTC 2 cut(s) 1190, 1222
BpmI CTGGAG 2 cut(s) 516, 645
Bpu14I TTCGAA 1 cut(s) 214
BpuEI CTTGAG 2 cut(s) 565, 1192
BsaJI CCNNGG 3 cut(s) 331, 458, 1563
BsaWI WCCGGW 3 cut(s) 733, 998, 1327
BsaXI ACNNNNNCTCC 4 cut(s) 489, 519, 576, 606
Bsc4I CCNNNNNNNGG 2 cut(s) 183, 563
BseAI TCCGGA 1 cut(s) 998
BseBI CCWGG 1 cut(s) 36
BseDI CCNNGG 3 cut(s) 331, 458, 1563
BseGI GGATG 5 cut(s) 995, 1028, 1350, 1466, 1473
BseLI CCNNNNNNNGG 2 cut(s) 183, 563
BseMII CTCAG 1 cut(s) 110
BseRI GAGGAG 1 cut(s) 597
Bsh1236I CGCG 1 cut(s) 131
BshFI GGCC 1 cut(s) 165
BsiSI CCGG 3 cut(s) 734, 999, 1328
BslFI GGGAC 1 cut(s) 291
BslI CCNNNNNNNGG 2 cut(s) 183, 563
BsmAI GTCTC 6 cut(s) 163, 205, 541, 723, 856, 1156
BsmFI GGGAC 1 cut(s) 291
BsnI GGCC 1 cut(s) 165
Bsp119I TTCGAA 1 cut(s) 214
Bsp13I TCCGGA 1 cut(s) 998
Bsp143I GATC 8 cut(s) 122, 208, 394, 491, 527, 648, 826, 1116
BspACI CCGC 1 cut(s) 1417
BspANI GGCC 1 cut(s) 165
BspCNI CTCAG 1 cut(s) 111
BspEI TCCGGA 1 cut(s) 998
BspFNI CGCG 1 cut(s) 131
BspLI GGNNCC 2 cut(s) 279, 1332
BspPI GGATC 4 cut(s) 389, 499, 821, 1111
BspT104I TTCGAA 1 cut(s) 214
BspTI CTTAAG 1 cut(s) 1550
BssECI CCNNGG 3 cut(s) 331, 458, 1563
BssMI GATC 8 cut(s) 122, 208, 394, 491, 527, 648, 826, 1116
BssSI CACGAG 2 cut(s) 390, 1339
BssT1I CCWWGG 2 cut(s) 458, 1563
Bst2BI CACGAG 2 cut(s) 390, 1339
Bst2UI CCWGG 1 cut(s) 36
Bst4CI ACNGT 4 cut(s) 182, 273, 571, 882
Bst6I CTCTTC 3 cut(s) 33, 726, 1176
BstAFI CTTAAG 1 cut(s) 1550
BstBI TTCGAA 1 cut(s) 214
BstDEI CTNAG 2 cut(s) 119, 1052
BstF5I GGATG 5 cut(s) 995, 1028, 1350, 1466, 1473
BstFNI CGCG 1 cut(s) 131
BstHHI GCGC 3 cut(s) 7, 424, 753
BstKTI GATC 8 cut(s) 125, 211, 397, 494, 530, 651, 829, 1119
BstMAI GTCTC 6 cut(s) 163, 205, 541, 723, 856, 1156
BstMBI GATC 8 cut(s) 122, 208, 394, 491, 527, 648, 826, 1116
BstMWI GCNNNNNNNGC 1 cut(s) 419
BstNI CCWGG 1 cut(s) 36
BstSCI CCNGG 1 cut(s) 34
BstSFI CTRYAG 2 cut(s) 63, 1036
BstUI CGCG 1 cut(s) 131
BstV2I GAAGAC 4 cut(s) 113, 375, 807, 1316
BstX2I RGATCY 3 cut(s) 394, 491, 826
BstXI CCANNNNNNTGG 1 cut(s) 1513
BstYI RGATCY 3 cut(s) 394, 491, 826
BsuI GTATCC 1 cut(s) 1199
BsuRI GGCC 1 cut(s) 165
BtgZI GCGATG 2 cut(s) 756, 1191
BtsCI GGATG 5 cut(s) 995, 1028, 1350, 1466, 1473
BtsIMutI CAGTG 2 cut(s) 269, 878
CfoI GCGC 3 cut(s) 7, 424, 753
Cfr13I GGNCC 1 cut(s) 278
CseI GACGC 4 cut(s) 120, 137, 1310, 1534
CspCI CAANNNNNGTGG 2 cut(s) 910, 945
CviAII CATG 6 cut(s) 206, 263, 531, 966, 1391, 1510
DdeI CTNAG 2 cut(s) 119, 1052
DpnI GATC 8 cut(s) 124, 210, 396, 493, 529, 650, 828, 1118
DpnII GATC 8 cut(s) 122, 208, 394, 491, 527, 648, 826, 1116
EaeI YGGCCR 1 cut(s) 163
Eam1104I CTCTTC 3 cut(s) 33, 726, 1176
EarI CTCTTC 3 cut(s) 33, 726, 1176
Eco130I CCWWGG 2 cut(s) 458, 1563
Eco47I GGWCC 1 cut(s) 278
EcoO109I RGGNCCY 1 cut(s) 278
EcoRII CCWGG 1 cut(s) 34
EcoT14I CCWWGG 2 cut(s) 458, 1563
ErhI CCWWGG 2 cut(s) 458, 1563
FaeI CATG 6 cut(s) 209, 266, 534, 969, 1394, 1513
FalI AAGNNNNNCTT 4 cut(s) 705, 737, 1204, 1236
FaqI GGGAC 1 cut(s) 291
FatI CATG 6 cut(s) 205, 262, 530, 965, 1390, 1509
FbaI TGATCA 1 cut(s) 648
FblI GTMKAC 3 cut(s) 433, 865, 918
FokI GGATG 5 cut(s) 982, 1035, 1357, 1460, 1473
FspBI CTAG 2 cut(s) 282, 1574
GlaI GCGC 3 cut(s) 6, 423, 752
GsuI CTGGAG 2 cut(s) 516, 645
HaeIII GGCC 1 cut(s) 165
HapII CCGG 3 cut(s) 734, 999, 1328
HgaI GACGC 4 cut(s) 120, 137, 1310, 1534
HhaI GCGC 3 cut(s) 7, 424, 753
Hin1II CATG 6 cut(s) 209, 266, 534, 969, 1394, 1513
Hin6I GCGC 3 cut(s) 5, 422, 751
HinP1I GCGC 3 cut(s) 5, 422, 751
HincII GTYRAC 1 cut(s) 1407
HindII GTYRAC 1 cut(s) 1407
HpaI GTTAAC 1 cut(s) 1407
HpaII CCGG 3 cut(s) 734, 999, 1328
HphI GGTGA 5 cut(s) 166, 233, 347, 604, 1579
Hpy166II GTNNAC 4 cut(s) 434, 866, 919, 1407
Hpy188I TCNGA 6 cut(s) 127, 239, 491, 510, 945, 1231
Hpy188III TCNNGA 7 cut(s) 76, 390, 495, 725, 999, 1209, 1339
Hpy8I GTNNAC 4 cut(s) 434, 866, 919, 1407
Hpy99I CGWCG 2 cut(s) 182, 1199
HpyAV CCTTC 7 cut(s) 79, 83, 349, 435, 769, 867, 1486
HpyCH4III ACNGT 4 cut(s) 182, 273, 571, 882
HpyCH4IV ACGT 3 cut(s) 436, 676, 1111
HpyCH4V TGCA 1 cut(s) 746
HpyF10VI GCNNNNNNNGC 1 cut(s) 419
HpyF3I CTNAG 2 cut(s) 119, 1052
HpySE526I ACGT 3 cut(s) 436, 676, 1111
Hsp92II CATG 6 cut(s) 209, 266, 534, 969, 1394, 1513
HspAI GCGC 3 cut(s) 5, 422, 751
Kpn2I TCCGGA 1 cut(s) 998
Ksp22I TGATCA 1 cut(s) 648
KspAI GTTAAC 1 cut(s) 1407
Kzo9I GATC 8 cut(s) 122, 208, 394, 491, 527, 648, 826, 1116
LmnI GCTCC 1 cut(s) 1565
LweI GCATC 3 cut(s) 16, 595, 733
MaeI CTAG 2 cut(s) 282, 1574
MaeII ACGT 3 cut(s) 436, 676, 1111
MaeIII GTNAC 7 cut(s) 221, 248, 265, 335, 681, 796, 1243
MalI GATC 8 cut(s) 124, 210, 396, 493, 529, 650, 828, 1118
MboI GATC 8 cut(s) 122, 208, 394, 491, 527, 648, 826, 1116
MflI RGATCY 3 cut(s) 394, 491, 826
MlsI TGGCCA 1 cut(s) 165
MluCI AATT 2 cut(s) 57, 535
MluI ACGCGT 1 cut(s) 129
MluNI TGGCCA 1 cut(s) 165
MlyI GAGTC 7 cut(s) 165, 389, 439, 821, 871, 1369, 1405
MmeI TCCRAC 4 cut(s) 164, 469, 1362, 1494
Mox20I TGGCCA 1 cut(s) 165
MroI TCCGGA 1 cut(s) 998
MroXI GAANNNNTTC 1 cut(s) 765
MscI TGGCCA 1 cut(s) 165
MseI TTAA 6 cut(s) 540, 618, 948, 1250, 1406, 1551
MslI CAYNNNNRTG 1 cut(s) 1343
Msp20I TGGCCA 1 cut(s) 165
MspCI CTTAAG 1 cut(s) 1550
MspI CCGG 3 cut(s) 734, 999, 1328
MspR9I CCNGG 1 cut(s) 36
MvaI CCWGG 1 cut(s) 36
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 1 cut(s) 419
NdeII GATC 8 cut(s) 122, 208, 394, 491, 527, 648, 826, 1116
NlaIII CATG 6 cut(s) 209, 266, 534, 969, 1394, 1513
NlaIV GGNNCC 2 cut(s) 279, 1332
NmuCI GTSAC 4 cut(s) 221, 248, 335, 1243
NspV TTCGAA 1 cut(s) 214
PdmI GAANNNNTTC 1 cut(s) 765
PfeI GAWTC 9 cut(s) 216, 295, 361, 766, 940, 1294, 1307, 1470, 1502
PleI GAGTC 7 cut(s) 165, 388, 438, 820, 870, 1369, 1405
PpsI GAGTC 7 cut(s) 165, 388, 438, 820, 870, 1369, 1405
PpuMI RGGWCCY 1 cut(s) 278
PshBI ATTAAT 1 cut(s) 948
Psp5II RGGWCCY 1 cut(s) 278
Psp6I CCWGG 1 cut(s) 34
PspGI CCWGG 1 cut(s) 34
PspN4I GGNNCC 2 cut(s) 279, 1332
PspPI GGNCC 1 cut(s) 278
PspPPI RGGWCCY 1 cut(s) 278
PsuI RGATCY 3 cut(s) 394, 491, 826
RseI CAYNNNNRTG 1 cut(s) 1343
SaqAI TTAA 6 cut(s) 540, 618, 948, 1250, 1406, 1551
Sau3AI GATC 8 cut(s) 122, 208, 394, 491, 527, 648, 826, 1116
Sau96I GGNCC 1 cut(s) 278
SchI GAGTC 7 cut(s) 165, 389, 439, 821, 871, 1369, 1405
ScrFI CCNGG 1 cut(s) 36
SfaNI GCATC 3 cut(s) 16, 595, 733
SfcI CTRYAG 2 cut(s) 63, 1036
SfuI TTCGAA 1 cut(s) 214
SinI GGWCC 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 1343
SmlI CTYRAG 3 cut(s) 544, 1207, 1550
SmoI CTYRAG 3 cut(s) 544, 1207, 1550
Sse9I AATT 2 cut(s) 57, 535
SsiI CCGC 1 cut(s) 1417
SspMI CTAG 2 cut(s) 282, 1574
StyD4I CCNGG 1 cut(s) 34
StyI CCWWGG 2 cut(s) 458, 1563
TaaI ACNGT 4 cut(s) 182, 273, 571, 882
TaiI ACGT 3 cut(s) 439, 679, 1114
TaqI TCGA 6 cut(s) 214, 318, 428, 860, 1160, 1422
TasI AATT 2 cut(s) 57, 535
TfiI GAWTC 9 cut(s) 216, 295, 361, 766, 940, 1294, 1307, 1470, 1502
Tru1I TTAA 6 cut(s) 540, 618, 948, 1250, 1406, 1551
Tru9I TTAA 6 cut(s) 540, 618, 948, 1250, 1406, 1551
TscAI CASTG 2 cut(s) 276, 885
TseFI GTSAC 4 cut(s) 221, 248, 335, 1243
Tsp45I GTSAC 4 cut(s) 221, 248, 335, 1243
TspDTI ATGAA 9 cut(s) 527, 531, 726, 966, 1158, 1194, 1361, 1374, 1462
TspGWI ACGGA 1 cut(s) 579
TspRI CASTG 2 cut(s) 276, 885
Vha464I CTTAAG 1 cut(s) 1550
VpaK11BI GGWCC 1 cut(s) 278
VspI ATTAAT 1 cut(s) 948
XapI RAATTY 1 cut(s) 535
XmiI GTMKAC 3 cut(s) 433, 865, 918
XmnI GAANNNNTTC 1 cut(s) 765
XspI CTAG 2 cut(s) 282, 1574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.