AT2G25260

hydroxyproline O-arabinosyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002578
Physical Location & Seq
Reverse (-)
10755524 .. 10757947
2424 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G25260.1

Sequence Viewer

Length: 1077 bp
ATGGGATTCCGTGGGAAGTACTTCTTCCCAATTCTCATGACTCTATCGTTATTTCTAATAATCAGATACAACTACATAGTCTCCGATGATCCTCCTCTCCGGCAAGAACTCCCCGGTCGCCGATCAGCTTCTTCCGGAGATGACATCACATATACCGTCAAGACTCCGTCAAAGAAGACAAAGAGACTGTTTCACACGGCGGTGACAGCCACGGATTCCGTGTACAGCACGTGGCAGTGTCGGGTCATGTATTATTGGTACAACAGGTTCAGGGACGAACCCGGGTCGGATATGGGCGGGTATACCCGGATCTTGCATTCGGGTCGACCCGATGGACTCATGGATGAGATCCCTACTTTCGTTGCTGACCCTCTTCCCTCCGGTGTTGACAAGGGATATGTTGTTCTAAATAGGCCTTGGGCCTTTGTTCAATGGCTTCAACAAGCACATATAGAGGAAGATTACATTCTCATGGCGGAACCTGATCATATCATAGTTAAACCGATACCAAACCTAGCTAGAGGCAATCTCGCGGCTGCGTTTCCCTTTTTCTACATTGAACCAAAGAAATATGAATCGGTTCTCCGCAAATTCTTCCCCAAAGAGAATGGACCAATATCAAGAATCGATCCCATCGGGAATTCTCCAGTGATCGTCACTAAGAATGCATTAATGAAAATTGCTCCAACATGGATGAATGTTTCTTTGGCTATGAAGAATGACCCACAAACAGATAAAGCTTTTGGTTGGGTCCTTGAAATGTATGCATATGCTGTTTCTTCTGCGTTACACGGCGTGAGCAACATTCTACACAAGGACTTCATGATTCAGCCTCCTTGGGACACTGAAACAAAGAAGACGTTCATAATACATTACACTTACGGCTGTGATTTCGACATGAAGGGGAAGATGATGGTAGGGAAGATTGGAGAATGGAGATTCGATAAACGGTCTTACGGGGATAAACCACCCCCAAGAAATCTCACACTGCCTCCCCGAGGTGTTCCCGAGAGCGTGGTGACATTGGTGACAATGATAAACGAAGCTACTGCTAATATTCCCAACTGGGAATCTTGA

Protein Analysis

358

Amino Acids

40.97

Weight (kDa)

8.91

Isoelectric Point (pI)

45.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HPAT PF23452 64 - 357 9e-129 Hydroxyproline O-arabinosyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 302, 325
AccII CGCG 1 cut(s) 533
AccIII TCCGGA 1 cut(s) 134
AciI CCGC 5 cut(s) 200, 297, 476, 533, 586
AclWI GGATC 4 cut(s) 83, 317, 343, 623
AcsI RAATTY 2 cut(s) 590, 640
AcvI CACGTG 1 cut(s) 231
AdeI CACNNNGTG 1 cut(s) 796
AfaI GTAC 3 cut(s) 20, 224, 260
AfiI CCNNNNNNNGG 1 cut(s) 998
AgsI TTSAA 4 cut(s) 431, 440, 560, 758
AjuI GAANNNNNNNTTGG 2 cut(s) 689, 721
AleI CACNNNNGTG 1 cut(s) 200
AloI GAACNNNNNNTCC 2 cut(s) 387, 419
AluBI AGCT 4 cut(s) 128, 518, 740, 1046
AluI AGCT 4 cut(s) 128, 518, 740, 1046
Alw26I GTCTC 2 cut(s) 85, 178
AlwI GGATC 4 cut(s) 83, 317, 343, 623
Ama87I CYCGRG 3 cut(s) 281, 996, 1007
Aor13HI TCCGGA 1 cut(s) 134
AoxI GGCC 2 cut(s) 413, 420
ApeKI GCWGC 1 cut(s) 536
ApoI RAATTY 2 cut(s) 590, 640
AseI ATTAAT 1 cut(s) 671
Asp700I GAANNNNTTC 3 cut(s) 20, 579, 860
AspS9I GGNCC 3 cut(s) 420, 611, 751
AsuC2I CCSGG 4 cut(s) 114, 282, 283, 307
AsuHPI GGTGA 3 cut(s) 214, 1030, 1039
AvaI CYCGRG 3 cut(s) 281, 996, 1007
AvaII GGWCC 2 cut(s) 611, 751
BbrPI CACGTG 1 cut(s) 231
BbsI GAAGAC 2 cut(s) 182, 863
BbvI GCAGC 1 cut(s) 523
BccI CCATC 3 cut(s) 326, 641, 907
BceAI ACGGC 3 cut(s) 213, 808, 898
BcgI CGANNNNNNTGC 4 cut(s) 305, 339, 1031, 1065
BclI TGATCA 1 cut(s) 484
BcnI CCSGG 4 cut(s) 114, 282, 283, 307
BcoDI GTCTC 2 cut(s) 85, 178
BfaI CTAG 2 cut(s) 515, 519
BisI GCNGC 2 cut(s) 534, 537
BlsI GCNGC 2 cut(s) 535, 538
BmcAI AGTACT 1 cut(s) 20
Bme1390I CCNGG 4 cut(s) 114, 282, 283, 307
Bme18I GGWCC 2 cut(s) 611, 751
BmeT110I CYCGRG 3 cut(s) 281, 996, 1007
BmgT120I GGNCC 3 cut(s) 420, 611, 751
BmiI GGNNCC 2 cut(s) 480, 752
BmrFI CCNGG 4 cut(s) 114, 282, 283, 307
BmrI ACTGGG 1 cut(s) 1075
BmuI ACTGGG 1 cut(s) 1075
BpiI GAAGAC 2 cut(s) 182, 863
BplI GAGNNNNNCTC 2 cut(s) 513, 545
BpmI CTGGAG 1 cut(s) 630
BpuMI CCSGG 4 cut(s) 114, 282, 283, 307
Bsa29I ATCGAT 1 cut(s) 627
BsaAI YACGTR 1 cut(s) 231
BsaJI CCNNGG 7 cut(s) 10, 112, 210, 281, 416, 836, 997
BsaWI WCCGGW 2 cut(s) 134, 380
BsaXI ACNNNNNCTCC 4 cut(s) 65, 95, 976, 1006
Bsc4I CCNNNNNNNGG 1 cut(s) 998
Bse1I ACTGG 2 cut(s) 647, 1070
BseAI TCCGGA 1 cut(s) 134
BseCI ATCGAT 1 cut(s) 627
BseDI CCNNGG 7 cut(s) 10, 112, 210, 281, 416, 836, 997
BseGI GGATG 2 cut(s) 349, 699
BseLI CCNNNNNNNGG 1 cut(s) 998
BseNI ACTGG 2 cut(s) 647, 1070
BseRI GAGGAG 1 cut(s) 84
BseXI GCAGC 1 cut(s) 523
Bsh1236I CGCG 1 cut(s) 533
Bsh1285I CGRYCG 1 cut(s) 118
BshFI GGCC 2 cut(s) 415, 422
BshVI ATCGAT 1 cut(s) 627
BsiEI CGRYCG 1 cut(s) 118
BsiHKCI CYCGRG 3 cut(s) 281, 996, 1007
BsiSI CCGG 6 cut(s) 100, 114, 135, 282, 307, 381
BslFI GGGAC 2 cut(s) 287, 854
BslI CCNNNNNNNGG 1 cut(s) 998
BsmAI GTCTC 2 cut(s) 85, 178
BsmFI GGGAC 2 cut(s) 287, 854
BsmI GAATGC 2 cut(s) 316, 670
BsnI GGCC 2 cut(s) 415, 422
BsoBI CYCGRG 3 cut(s) 281, 996, 1007
Bsp13I TCCGGA 1 cut(s) 134
Bsp1407I TGTACA 1 cut(s) 222
Bsp143I GATC 7 cut(s) 88, 122, 309, 348, 484, 628, 651
BspACI CCGC 5 cut(s) 200, 297, 476, 533, 586
BspANI GGCC 2 cut(s) 415, 422
BspDI ATCGAT 1 cut(s) 627
BspEI TCCGGA 1 cut(s) 134
BspFNI CGCG 1 cut(s) 533
BspHI TCATGA 2 cut(s) 36, 822
BspLI GGNNCC 2 cut(s) 480, 752
BspPI GGATC 4 cut(s) 83, 317, 343, 623
BsrGI TGTACA 1 cut(s) 222
BsrI ACTGG 2 cut(s) 647, 1070
BssECI CCNNGG 7 cut(s) 10, 112, 210, 281, 416, 836, 997
BssMI GATC 7 cut(s) 88, 122, 309, 348, 484, 628, 651
BssNAI GTATAC 1 cut(s) 303
BssT1I CCWWGG 2 cut(s) 416, 836
Bst1107I GTATAC 1 cut(s) 303
Bst4CI ACNGT 3 cut(s) 157, 189, 951
Bst6I CTCTTC 1 cut(s) 378
BstAUI TGTACA 1 cut(s) 222
BstBAI YACGTR 1 cut(s) 231
BstDEI CTNAG 1 cut(s) 660
BstDSI CCRYGG 2 cut(s) 10, 210
BstENI CCTNNNNNAGG 1 cut(s) 996
BstF5I GGATG 2 cut(s) 349, 699
BstFNI CGCG 1 cut(s) 533
BstKTI GATC 7 cut(s) 91, 125, 312, 351, 487, 631, 654
BstMAI GTCTC 2 cut(s) 85, 178
BstMBI GATC 7 cut(s) 88, 122, 309, 348, 484, 628, 651
BstMCI CGRYCG 1 cut(s) 118
BstMWI GCNNNNNNNGC 1 cut(s) 206
BstSCI CCNGG 4 cut(s) 112, 280, 281, 305
BstUI CGCG 1 cut(s) 533
BstV1I GCAGC 1 cut(s) 523
BstV2I GAAGAC 2 cut(s) 182, 863
BstX2I RGATCY 2 cut(s) 309, 348
BstYI RGATCY 2 cut(s) 309, 348
BstZ17I GTATAC 1 cut(s) 303
Bsu15I ATCGAT 1 cut(s) 627
BsuRI GGCC 2 cut(s) 415, 422
BsuTUI ATCGAT 1 cut(s) 627
BtgI CCRYGG 2 cut(s) 10, 210
BtsCI GGATG 2 cut(s) 349, 699
BtsI GCAGTG 2 cut(s) 242, 986
BtsIMutI CAGTG 4 cut(s) 242, 654, 843, 986
CciI TCATGA 2 cut(s) 36, 822
Cfr13I GGNCC 3 cut(s) 420, 611, 751
Cfr9I CCCGGG 1 cut(s) 281
ClaI ATCGAT 1 cut(s) 627
Csp6I GTAC 3 cut(s) 19, 223, 259
CviAII CATG 7 cut(s) 37, 247, 340, 472, 690, 823, 898
CviQI GTAC 3 cut(s) 19, 223, 259
DdeI CTNAG 1 cut(s) 660
DpnI GATC 7 cut(s) 90, 124, 311, 350, 486, 630, 653
DpnII GATC 7 cut(s) 88, 122, 309, 348, 484, 628, 651
DraIII CACNNNGTG 1 cut(s) 796
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
EciI GGCGGA 1 cut(s) 491
Eco130I CCWWGG 2 cut(s) 416, 836
Eco147I AGGCCT 1 cut(s) 415
Eco47I GGWCC 2 cut(s) 611, 751
Eco72I CACGTG 1 cut(s) 231
Eco88I CYCGRG 3 cut(s) 281, 996, 1007
EcoNI CCTNNNNNAGG 1 cut(s) 996
EcoO109I RGGNCCY 1 cut(s) 751
EcoRI GAATTC 1 cut(s) 640
EcoT14I CCWWGG 2 cut(s) 416, 836
EcoT22I ATGCAT 2 cut(s) 670, 769
ErhI CCWWGG 2 cut(s) 416, 836
FaeI CATG 7 cut(s) 40, 250, 343, 475, 693, 826, 901
FalI AAGNNNNNCTT 2 cut(s) 8, 40
FaqI GGGAC 2 cut(s) 287, 854
FatI CATG 7 cut(s) 36, 246, 339, 471, 689, 822, 897
FauI CCCGC 1 cut(s) 290
FauNDI CATATG 1 cut(s) 769
FbaI TGATCA 1 cut(s) 484
FblI GTMKAC 2 cut(s) 302, 325
Fnu4HI GCNGC 2 cut(s) 534, 537
FokI GGATG 2 cut(s) 356, 706
Fsp4HI GCNGC 2 cut(s) 534, 537
FspBI CTAG 2 cut(s) 515, 519
GluI GCNGC 2 cut(s) 534, 537
GsuI CTGGAG 1 cut(s) 630
HaeIII GGCC 2 cut(s) 415, 422
HapII CCGG 6 cut(s) 100, 114, 135, 282, 307, 381
Hin1II CATG 7 cut(s) 40, 250, 343, 475, 693, 826, 901
HincII GTYRAC 2 cut(s) 326, 388
HindII GTYRAC 2 cut(s) 326, 388
HindIII AAGCTT 1 cut(s) 738
HpaII CCGG 6 cut(s) 100, 114, 135, 282, 307, 381
HphI GGTGA 3 cut(s) 214, 1030, 1039
Hpy166II GTNNAC 4 cut(s) 223, 303, 326, 388
Hpy188I TCNGA 3 cut(s) 65, 85, 289
Hpy188III TCNNGA 8 cut(s) 37, 135, 160, 621, 637, 823, 1007, 1074
Hpy8I GTNNAC 4 cut(s) 223, 303, 326, 388
HpyAV CCTTC 1 cut(s) 895
HpyCH4III ACNGT 3 cut(s) 157, 189, 951
HpyCH4IV ACGT 2 cut(s) 230, 860
HpyCH4V TGCA 3 cut(s) 316, 668, 767
HpyF10VI GCNNNNNNNGC 1 cut(s) 206
HpyF3I CTNAG 1 cut(s) 660
HpySE526I ACGT 2 cut(s) 230, 860
Hsp92II CATG 7 cut(s) 40, 250, 343, 475, 693, 826, 901
Kpn2I TCCGGA 1 cut(s) 134
Ksp22I TGATCA 1 cut(s) 484
Kzo9I GATC 7 cut(s) 88, 122, 309, 348, 484, 628, 651
LmnI GCTCC 1 cut(s) 688
Lsp1109I GCAGC 1 cut(s) 523
MaeI CTAG 2 cut(s) 515, 519
MaeII ACGT 2 cut(s) 230, 860
MaeIII GTNAC 5 cut(s) 202, 655, 786, 1018, 1027
MalI GATC 7 cut(s) 90, 124, 311, 350, 486, 630, 653
MboI GATC 7 cut(s) 88, 122, 309, 348, 484, 628, 651
MflI RGATCY 2 cut(s) 309, 348
MluCI AATT 4 cut(s) 30, 590, 640, 678
MlyI GAGTC 3 cut(s) 34, 157, 330
MmeI TCCRAC 2 cut(s) 267, 710
MnlI CCTC 9 cut(s) 102, 105, 381, 388, 448, 515, 843, 992, 1002
Mph1103I ATGCAT 2 cut(s) 670, 769
MroI TCCGGA 1 cut(s) 134
MroXI GAANNNNTTC 3 cut(s) 20, 579, 860
MseI TTAA 2 cut(s) 498, 671
MslI CAYNNNNRTG 2 cut(s) 200, 470
MspI CCGG 6 cut(s) 100, 114, 135, 282, 307, 381
MspR9I CCNGG 4 cut(s) 114, 282, 283, 307
Mva1269I GAATGC 2 cut(s) 316, 670
MvnI CGCG 1 cut(s) 533
MwoI GCNNNNNNNGC 1 cut(s) 206
NciI CCSGG 4 cut(s) 114, 282, 283, 307
NdeI CATATG 1 cut(s) 769
NdeII GATC 7 cut(s) 88, 122, 309, 348, 484, 628, 651
NlaIII CATG 7 cut(s) 40, 250, 343, 475, 693, 826, 901
NlaIV GGNNCC 2 cut(s) 480, 752
NmuCI GTSAC 4 cut(s) 202, 655, 1018, 1027
NsiI ATGCAT 2 cut(s) 670, 769
OliI CACNNNNGTG 1 cut(s) 200
PagI TCATGA 2 cut(s) 36, 822
PceI AGGCCT 1 cut(s) 415
PctI GAATGC 2 cut(s) 316, 670
PdmI GAANNNNTTC 3 cut(s) 20, 579, 860
PfeI GAWTC 7 cut(s) 6, 215, 575, 624, 826, 939, 1070
PflFI GACNNNGTC 1 cut(s) 166
PkrI GCNGC 2 cut(s) 535, 538
PleI GAGTC 3 cut(s) 34, 157, 330
PmaCI CACGTG 1 cut(s) 231
PmlI CACGTG 1 cut(s) 231
PpsI GAGTC 3 cut(s) 34, 157, 330
Ppu21I YACGTR 1 cut(s) 231
PpuMI RGGWCCY 1 cut(s) 751
PshBI ATTAAT 1 cut(s) 671
Psp5II RGGWCCY 1 cut(s) 751
PspCI CACGTG 1 cut(s) 231
PspN4I GGNNCC 2 cut(s) 480, 752
PspPI GGNCC 3 cut(s) 420, 611, 751
PspPPI RGGWCCY 1 cut(s) 751
PsrI GAACNNNNNNTAC 2 cut(s) 251, 283
PsuI RGATCY 2 cut(s) 309, 348
PsyI GACNNNGTC 1 cut(s) 166
RsaI GTAC 3 cut(s) 20, 224, 260
RsaNI GTAC 3 cut(s) 19, 223, 259
RseI CAYNNNNRTG 2 cut(s) 200, 470
SalI GTCGAC 1 cut(s) 324
SaqAI TTAA 2 cut(s) 498, 671
SatI GCNGC 2 cut(s) 534, 537
Sau3AI GATC 7 cut(s) 88, 122, 309, 348, 484, 628, 651
Sau96I GGNCC 3 cut(s) 420, 611, 751
ScaI AGTACT 1 cut(s) 20
SchI GAGTC 3 cut(s) 34, 157, 330
ScrFI CCNGG 4 cut(s) 114, 282, 283, 307
SinI GGWCC 2 cut(s) 611, 751
SmaI CCCGGG 1 cut(s) 283
SmiMI CAYNNNNRTG 2 cut(s) 200, 470
Sse9I AATT 4 cut(s) 30, 590, 640, 678
SseBI AGGCCT 1 cut(s) 415
SsiI CCGC 5 cut(s) 200, 297, 476, 533, 586
SspI AATATT 1 cut(s) 1057
SspMI CTAG 2 cut(s) 515, 519
StuI AGGCCT 1 cut(s) 415
StyD4I CCNGG 4 cut(s) 112, 280, 281, 305
StyI CCWWGG 2 cut(s) 416, 836
TaaI ACNGT 3 cut(s) 157, 189, 951
TaiI ACGT 2 cut(s) 233, 863
TaqI TCGA 4 cut(s) 325, 627, 894, 942
TasI AATT 4 cut(s) 30, 590, 640, 678
TatI WGTACW 2 cut(s) 18, 222
TauI GCSGC 1 cut(s) 536
TfiI GAWTC 7 cut(s) 6, 215, 575, 624, 826, 939, 1070
Tru1I TTAA 2 cut(s) 498, 671
Tru9I TTAA 2 cut(s) 498, 671
TscAI CASTG 4 cut(s) 242, 654, 850, 993
TseFI GTSAC 4 cut(s) 202, 655, 1018, 1027
TseI GCWGC 1 cut(s) 536
Tsp45I GTSAC 4 cut(s) 202, 655, 1018, 1027
TspDTI ATGAA 7 cut(s) 588, 689, 710, 728, 811, 853, 914
TspGWI ACGGA 3 cut(s) 156, 208, 227
TspMI CCCGGG 1 cut(s) 281
TspRI CASTG 4 cut(s) 242, 654, 850, 993
Tth111I GACNNNGTC 1 cut(s) 166
VpaK11BI GGWCC 2 cut(s) 611, 751
VspI ATTAAT 1 cut(s) 671
XagI CCTNNNNNAGG 1 cut(s) 996
XapI RAATTY 2 cut(s) 590, 640
XmaI CCCGGG 1 cut(s) 281
XmiI GTMKAC 2 cut(s) 302, 325
XmnI GAANNNNTTC 3 cut(s) 20, 579, 860
XspI CTAG 2 cut(s) 515, 519
ZrmI AGTACT 1 cut(s) 20
Zsp2I ATGCAT 2 cut(s) 670, 769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.