MD03G1165800.v1.1

hydroxyproline O-arabinosyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002578
Physical Location & Seq
Reverse (-)
22016968 .. 22021128
4161 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1165800.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGATTAGGAGAAAAAGCATGGGACGTGCATCGCCAGTACTTTTGGTGCTTTTAGCACTTGGTTTTTTCTTTGCAACTTATAATTTGTTAACCATTATAATACACAACAAGGCCTCCTACTCGGGCATTTTGGGGGAATACCGGTTGGAAGATCCTGTGATTCAGCGGCCCTCGAAGGCGGAGAATCCAAAATTTCATGTTGTTGTTACAGCCACTGATGCTCCGTATAGCCAGTGGCAGTGTCGGATCATGTATTACTGGTACAACAAGGTAAAAGATATGCCTGGATCAGATATGGGAAAGTTCACTCGAGTTTTGCATTCTGGAAATGCTGACAATTTGACGGAGGAGATTCCAACAGTTGTGGTTGATCCTCTTCCGGAAGGCTTGGATCGGGGCTATATTGTCCTAAACAGACCATGGGCTTTTGTGCAATGGCTGGAGAAAGCAACAATCGAGGAAGAATACATTCTAATGGCTGAACCTGACCACATATTTGTAAATCCTTTGCCAAACTTAGCACGTGGAAACAATCCAGCAGGGTATCCATTTTTCTACATTAAACCAACCGAAAATGAGAAAATAATTAGAAAGTTTTATCCTGTGGAAAAAGGCCCTGTGACTGATGTTGATCCAATTGGCAATTCTCCTGTAATCATAAAGAAGTCCCTGCTGGAGGAAATCGCTCCCACATGGGTGAATGTGTCTTTGAAAATGAAAGATGACCTAGAGACTGATAAGGCTTTTGGATGGGTGCTTGAGATGTATGCATATGCTGTGGCATCTGCATTGCATGGTGTGCGGCATATTCTTCGTGAAGACTTTATGTTGCAGCCTCCATGGGATTTGAAAGTTGGGAAGAGGTTCATCATCCATTATACTTACGGATGCGACTATAATTTAAAAGGAGACCTGACTTATGGTAAGATTGGAGAATGGCGTTTTGACAAGAGAGCATATCTCAGTGGTCCTCCACCAAGAAACCTCTCTTTGCCCCCTCCAGGAGTTCCTGAAAGTGTGGTAAGGCTCGTGAAAATGGTTAACGAGGCTACTGCAAACATTCCTGGTTGGTACAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
No domains found.

Protein Analysis

360

Amino Acids

40.98

Weight (kDa)

7.7

Isoelectric Point (pI)

46.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HPAT PF23452 65 - 357 1.4e-125 Hydroxyproline O-arabinosyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 81, 98
AccIII TCCGGA 1 cut(s) 379
AciI CCGC 3 cut(s) 166, 179, 802
AclWI GGATC 6 cut(s) 146, 254, 295, 365, 399, 626
AcsI RAATTY 1 cut(s) 191
AcvI CACGTG 1 cut(s) 524
AfaI GTAC 3 cut(s) 39, 263, 1073
AfiI CCNNNNNNNGG 2 cut(s) 676, 1001
AgeI ACCGGT 1 cut(s) 141
AgsI TTSAA 2 cut(s) 712, 850
AjiI CACGTC 1 cut(s) 26
AjnI CCWGG 3 cut(s) 283, 1000, 1063
AjuI GAANNNNNNNTTGG 2 cut(s) 628, 660
AleI CACNNNNGTG 1 cut(s) 695
AluBI AGCT 1 cut(s) 1077
AluI AGCT 1 cut(s) 1077
Alw26I GTCTC 2 cut(s) 725, 903
AlwI GGATC 6 cut(s) 146, 254, 295, 365, 399, 626
AlwNI CAGNNNCTG 1 cut(s) 215
Ama87I CYCGRG 2 cut(s) 121, 309
Aor13HI TCCGGA 1 cut(s) 379
AoxI GGCC 3 cut(s) 111, 167, 613
ApeKI GCWGC 1 cut(s) 832
ApoI RAATTY 1 cut(s) 191
ArsI GACNNNNNNTTYG 2 cut(s) 479, 511
AsiGI ACCGGT 1 cut(s) 141
Asp700I GAANNNNTTC 2 cut(s) 468, 863
AspS9I GGNCC 3 cut(s) 168, 614, 968
AsuHPI GGTGA 1 cut(s) 709
AvaI CYCGRG 2 cut(s) 121, 309
AvaII GGWCC 1 cut(s) 968
BauI CACGAG 1 cut(s) 1028
BbrPI CACGTG 1 cut(s) 524
BbsI GAAGAC 1 cut(s) 825
BbvI GCAGC 1 cut(s) 844
BccI CCATC 1 cut(s) 744
BcgI CGANNNNNNTGC 4 cut(s) 794, 828, 1034, 1068
BciT130I CCWGG 3 cut(s) 285, 1002, 1065
BciVI GTATCC 1 cut(s) 555
BcoDI GTCTC 2 cut(s) 725, 903
BfaI CTAG 1 cut(s) 728
BfuI GTATCC 1 cut(s) 555
BisI GCNGC 3 cut(s) 167, 803, 833
BlsI GCNGC 3 cut(s) 168, 804, 834
BmcAI AGTACT 1 cut(s) 39
Bme1390I CCNGG 3 cut(s) 285, 1002, 1065
Bme18I GGWCC 1 cut(s) 968
BmeT110I CYCGRG 2 cut(s) 121, 309
BmgBI CACGTC 1 cut(s) 26
BmgT120I GGNCC 3 cut(s) 168, 614, 968
BmrFI CCNGG 3 cut(s) 285, 1002, 1065
BmsI GCATC 4 cut(s) 38, 208, 791, 878
BpiI GAAGAC 1 cut(s) 825
BplI GAGNNNNNCTC 2 cut(s) 945, 977
BpmI CTGGAG 3 cut(s) 461, 695, 984
BpuEI CTTGAG 1 cut(s) 779
BsaAI YACGTR 1 cut(s) 524
BsaBI GATNNNNATC 1 cut(s) 630
BsaI GGTCTC 1 cut(s) 903
BsaJI CCNNGG 2 cut(s) 419, 839
BsaWI WCCGGW 2 cut(s) 141, 379
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 205, 235
Bsc4I CCNNNNNNNGG 2 cut(s) 676, 1001
Bse118I RCCGGY 1 cut(s) 141
Bse1I ACTGG 3 cut(s) 35, 232, 263
Bse3DI GCAATG 2 cut(s) 440, 788
Bse8I GATNNNNATC 1 cut(s) 630
BseAI TCCGGA 1 cut(s) 379
BseBI CCWGG 3 cut(s) 285, 1002, 1065
BseDI CCNNGG 2 cut(s) 419, 839
BseGI GGATG 3 cut(s) 755, 870, 893
BseJI GATNNNNATC 1 cut(s) 630
BseLI CCNNNNNNNGG 2 cut(s) 676, 1001
BseMI GCAATG 2 cut(s) 440, 788
BseMII CTCAG 1 cut(s) 976
BseNI ACTGG 3 cut(s) 35, 232, 263
BseRI GAGGAG 1 cut(s) 362
BseXI GCAGC 1 cut(s) 844
BshFI GGCC 3 cut(s) 113, 169, 615
BshTI ACCGGT 1 cut(s) 141
BsiHKCI CYCGRG 2 cut(s) 121, 309
BsiSI CCGG 2 cut(s) 142, 380
BslFI GGGAC 2 cut(s) 36, 652
BslI CCNNNNNNNGG 2 cut(s) 676, 1001
BsmAI GTCTC 2 cut(s) 725, 903
BsmFI GGGAC 2 cut(s) 36, 652
BsmI GAATGC 1 cut(s) 319
BsnI GGCC 3 cut(s) 113, 169, 615
Bso31I GGTCTC 1 cut(s) 903
BsoBI CYCGRG 2 cut(s) 121, 309
Bsp13I TCCGGA 1 cut(s) 379
Bsp143I GATC 6 cut(s) 151, 246, 287, 370, 391, 631
Bsp19I CCATGG 2 cut(s) 419, 839
BspACI CCGC 3 cut(s) 166, 179, 802
BspANI GGCC 3 cut(s) 113, 169, 615
BspCNI CTCAG 1 cut(s) 975
BspEI TCCGGA 1 cut(s) 379
BspPI GGATC 6 cut(s) 146, 254, 295, 365, 399, 626
BspTNI GGTCTC 1 cut(s) 903
BsrDI GCAATG 2 cut(s) 440, 788
BsrFI RCCGGY 1 cut(s) 141
BsrI ACTGG 3 cut(s) 35, 232, 263
BssAI RCCGGY 1 cut(s) 141
BssECI CCNNGG 2 cut(s) 419, 839
BssMI GATC 6 cut(s) 151, 246, 287, 370, 391, 631
BssSI CACGAG 1 cut(s) 1028
BssT1I CCWWGG 2 cut(s) 419, 839
Bst2BI CACGAG 1 cut(s) 1028
Bst2UI CCWGG 3 cut(s) 285, 1002, 1065
Bst4CI ACNGT 1 cut(s) 361
Bst6I CTCTTC 2 cut(s) 381, 854
BstAPI GCANNNNNTGC 1 cut(s) 799
BstBAI YACGTR 1 cut(s) 524
BstDEI CTNAG 2 cut(s) 517, 962
BstDSI CCRYGG 2 cut(s) 419, 839
BstENI CCTNNNNNAGG 1 cut(s) 674
BstF5I GGATG 3 cut(s) 755, 870, 893
BstKTI GATC 6 cut(s) 154, 249, 290, 373, 394, 634
BstMAI GTCTC 2 cut(s) 725, 903
BstMBI GATC 6 cut(s) 151, 246, 287, 370, 391, 631
BstMWI GCNNNNNNNGC 2 cut(s) 218, 799
BstNI CCWGG 3 cut(s) 285, 1002, 1065
BstSCI CCNGG 3 cut(s) 283, 1000, 1063
BstV1I GCAGC 1 cut(s) 844
BstV2I GAAGAC 1 cut(s) 825
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BsuI GTATCC 1 cut(s) 555
BsuRI GGCC 3 cut(s) 113, 169, 615
BtgI CCRYGG 2 cut(s) 419, 839
BtgZI GCGATG 1 cut(s) 15
BtrI CACGTC 1 cut(s) 26
BtsCI GGATG 3 cut(s) 755, 870, 893
BtsI GCAGTG 1 cut(s) 245
BtsIMutI CAGTG 4 cut(s) 213, 239, 245, 970
CaiI CAGNNNCTG 1 cut(s) 215
Cfr10I RCCGGY 1 cut(s) 141
Cfr13I GGNCC 3 cut(s) 168, 614, 968
Csp6I GTAC 3 cut(s) 38, 262, 1072
CspAI ACCGGT 1 cut(s) 141
CspCI CAANNNNNGTGG 2 cut(s) 345, 380
CviAII CATG 7 cut(s) 19, 197, 250, 420, 693, 794, 840
CviQI GTAC 3 cut(s) 38, 262, 1072
DdeI CTNAG 2 cut(s) 517, 962
DpnI GATC 6 cut(s) 153, 248, 289, 372, 393, 633
DpnII GATC 6 cut(s) 151, 246, 287, 370, 391, 631
DraI TTTAAA 1 cut(s) 903
Eam1104I CTCTTC 2 cut(s) 381, 854
EarI CTCTTC 2 cut(s) 381, 854
EciI GGCGGA 1 cut(s) 194
Eco130I CCWWGG 2 cut(s) 419, 839
Eco147I AGGCCT 1 cut(s) 113
Eco31I GGTCTC 1 cut(s) 903
Eco47I GGWCC 1 cut(s) 968
Eco72I CACGTG 1 cut(s) 524
Eco88I CYCGRG 2 cut(s) 121, 309
EcoNI CCTNNNNNAGG 1 cut(s) 674
EcoO109I RGGNCCY 1 cut(s) 614
EcoRII CCWGG 3 cut(s) 283, 1000, 1063
EcoT14I CCWWGG 2 cut(s) 419, 839
EcoT22I ATGCAT 1 cut(s) 772
ErhI CCWWGG 2 cut(s) 419, 839
FaeI CATG 7 cut(s) 22, 200, 253, 423, 696, 797, 843
FaqI GGGAC 2 cut(s) 36, 652
FatI CATG 7 cut(s) 18, 196, 249, 419, 692, 793, 839
FauNDI CATATG 1 cut(s) 772
Fnu4HI GCNGC 3 cut(s) 167, 803, 833
FokI GGATG 3 cut(s) 762, 857, 900
Fsp4HI GCNGC 3 cut(s) 167, 803, 833
FspBI CTAG 1 cut(s) 728
GluI GCNGC 3 cut(s) 167, 803, 833
GsuI CTGGAG 3 cut(s) 461, 695, 984
HaeIII GGCC 3 cut(s) 113, 169, 615
HapII CCGG 2 cut(s) 142, 380
Hin1II CATG 7 cut(s) 22, 200, 253, 423, 696, 797, 843
HincII GTYRAC 2 cut(s) 90, 1042
HindII GTYRAC 2 cut(s) 90, 1042
HinfI GANTC 3 cut(s) 160, 184, 352
HpaI GTTAAC 2 cut(s) 90, 1042
HpaII CCGG 2 cut(s) 142, 380
HphI GGTGA 1 cut(s) 709
Hpy166II GTNNAC 3 cut(s) 90, 306, 1042
Hpy188I TCNGA 2 cut(s) 246, 292
Hpy188III TCNNGA 5 cut(s) 324, 380, 815, 1010, 1030
Hpy8I GTNNAC 3 cut(s) 90, 306, 1042
HpyAV CCTTC 2 cut(s) 169, 377
HpyCH4III ACNGT 1 cut(s) 361
HpyCH4IV ACGT 2 cut(s) 25, 523
HpyCH4V TGCA 9 cut(s) 29, 74, 319, 433, 770, 788, 793, 832, 1055
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 799
HpyF3I CTNAG 2 cut(s) 517, 962
HpySE526I ACGT 2 cut(s) 25, 523
Hsp92II CATG 7 cut(s) 22, 200, 253, 423, 696, 797, 843
Kpn2I TCCGGA 1 cut(s) 379
KspAI GTTAAC 2 cut(s) 90, 1042
Kzo9I GATC 6 cut(s) 151, 246, 287, 370, 391, 631
LmnI GCTCC 2 cut(s) 226, 691
Lsp1109I GCAGC 1 cut(s) 844
LweI GCATC 4 cut(s) 38, 208, 791, 878
MaeI CTAG 1 cut(s) 728
MaeII ACGT 2 cut(s) 25, 523
MaeIII GTNAC 2 cut(s) 205, 619
MalI GATC 6 cut(s) 153, 248, 289, 372, 393, 633
MboI GATC 6 cut(s) 151, 246, 287, 370, 391, 631
MboII GAAGA 6 cut(s) 161, 368, 473, 803, 830, 871
MfeI CAATTG 1 cut(s) 636
MflI RGATCY 1 cut(s) 151
MluCI AATT 7 cut(s) 82, 191, 337, 585, 636, 643, 898
MmeI TCCRAC 3 cut(s) 126, 224, 380
Mph1103I ATGCAT 1 cut(s) 772
MroI TCCGGA 1 cut(s) 379
MroXI GAANNNNTTC 2 cut(s) 468, 863
MseI TTAA 4 cut(s) 89, 561, 902, 1041
MslI CAYNNNNRTG 2 cut(s) 473, 695
MspA1I CMGCKG 1 cut(s) 166
MspI CCGG 2 cut(s) 142, 380
MspR9I CCNGG 3 cut(s) 285, 1002, 1065
MunI CAATTG 1 cut(s) 636
Mva1269I GAATGC 1 cut(s) 319
MvaI CCWGG 3 cut(s) 285, 1002, 1065
MwoI GCNNNNNNNGC 2 cut(s) 218, 799
NcoI CCATGG 2 cut(s) 419, 839
NdeI CATATG 1 cut(s) 772
NdeII GATC 6 cut(s) 151, 246, 287, 370, 391, 631
NlaIII CATG 7 cut(s) 22, 200, 253, 423, 696, 797, 843
NmuCI GTSAC 1 cut(s) 619
NsiI ATGCAT 1 cut(s) 772
OliI CACNNNNGTG 1 cut(s) 695
PaeR7I CTCGAG 1 cut(s) 309
PceI AGGCCT 1 cut(s) 113
PctI GAATGC 1 cut(s) 319
PdmI GAANNNNTTC 2 cut(s) 468, 863
PfeI GAWTC 3 cut(s) 160, 184, 352
PfoI TCCNGGA 1 cut(s) 1000
PinAI ACCGGT 1 cut(s) 141
PkrI GCNGC 3 cut(s) 168, 804, 834
PmaCI CACGTG 1 cut(s) 524
PmlI CACGTG 1 cut(s) 524
Ppu21I YACGTR 1 cut(s) 524
PsiI TTATAA 2 cut(s) 81, 98
Psp6I CCWGG 3 cut(s) 283, 1000, 1063
PspCI CACGTG 1 cut(s) 524
PspGI CCWGG 3 cut(s) 283, 1000, 1063
PspPI GGNCC 3 cut(s) 168, 614, 968
PspXI VCTCGAGB 1 cut(s) 309
PstNI CAGNNNCTG 1 cut(s) 215
PsuI RGATCY 1 cut(s) 151
RsaI GTAC 3 cut(s) 39, 263, 1073
RsaNI GTAC 3 cut(s) 38, 262, 1072
RseI CAYNNNNRTG 2 cut(s) 473, 695
SaqAI TTAA 4 cut(s) 89, 561, 902, 1041
SatI GCNGC 3 cut(s) 167, 803, 833
Sau3AI GATC 6 cut(s) 151, 246, 287, 370, 391, 631
Sau96I GGNCC 3 cut(s) 168, 614, 968
ScaI AGTACT 1 cut(s) 39
ScrFI CCNGG 3 cut(s) 285, 1002, 1065
SetI ASST 9 cut(s) 28, 273, 487, 526, 729, 866, 915, 987, 1079
SfaNI GCATC 4 cut(s) 38, 208, 791, 878
Sfr274I CTCGAG 1 cut(s) 309
SinI GGWCC 1 cut(s) 968
SlaI CTCGAG 1 cut(s) 309
SmiMI CAYNNNNRTG 2 cut(s) 473, 695
SmlI CTYRAG 2 cut(s) 309, 758
SmoI CTYRAG 2 cut(s) 309, 758
Sse9I AATT 7 cut(s) 82, 191, 337, 585, 636, 643, 898
SseBI AGGCCT 1 cut(s) 113
SsiI CCGC 3 cut(s) 166, 179, 802
SspMI CTAG 1 cut(s) 728
StuI AGGCCT 1 cut(s) 113
StyD4I CCNGG 3 cut(s) 283, 1000, 1063
StyI CCWWGG 2 cut(s) 419, 839
TaaI ACNGT 1 cut(s) 361
TaiI ACGT 2 cut(s) 28, 526
TaqI TCGA 3 cut(s) 173, 310, 456
TasI AATT 7 cut(s) 82, 191, 337, 585, 636, 643, 898
TatI WGTACW 1 cut(s) 37
TauI GCSGC 2 cut(s) 169, 805
TfiI GAWTC 3 cut(s) 160, 184, 352
Tru1I TTAA 4 cut(s) 89, 561, 902, 1041
Tru9I TTAA 4 cut(s) 89, 561, 902, 1041
TscAI CASTG 4 cut(s) 220, 239, 245, 970
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 1 cut(s) 832
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 3 cut(s) 185, 731, 856
TspGWI ACGGA 3 cut(s) 213, 359, 900
TspRI CASTG 4 cut(s) 220, 239, 245, 970
VpaK11BI GGWCC 1 cut(s) 968
XagI CCTNNNNNAGG 1 cut(s) 674
XapI RAATTY 1 cut(s) 191
XhoI CTCGAG 1 cut(s) 309
XmnI GAANNNNTTC 2 cut(s) 468, 863
XspI CTAG 1 cut(s) 728
ZrmI AGTACT 1 cut(s) 39
Zsp2I ATGCAT 1 cut(s) 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.